P20816 (CP4A2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 4A2

Alternative name(s):
CYPIVA2
Cytochrome P-450 K-2
Cytochrome P450 K-5
Cytochrome P450-LA-omega 2
Lauric acid omega-hydroxylase
EC=1.14.15.3

Gene names

Name:	Cyp4a2
Synonyms:	Cyp4a-2, Cyp4a11

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	Octane + reduced rubredoxin + O2 = 1-octanol + oxidized rubredoxin + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By clofibrate.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	arachidonic acid metabolic process Inferred from mutant phenotype. Source: RGD icosanoid biosynthetic process Inferred from mutant phenotype. Source: RGD kidney development Inferred from expression pattern. Source: RGD lauric acid metabolic process Inferred from direct assay. Source: RGD linoleic acid metabolic process Inferred from direct assay. Source: RGD response to drug Inferred from expression pattern. Source: RGD response to hormone stimulus Inferred from expression pattern. Source: RGD
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from direct assay. Source: RGD
Molecular function	alkane 1-monooxygenase activity Inferred from mutant phenotype. Source: RGD arachidonic acid 11,12-epoxygenase activity Traceable author statement. Source: RGD arachidonic acid binding Inferred from direct assay. Source: RGD electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 4	4			PRO_0000003567
	Chain	5 – 504	500	Cytochrome P450 4A2		PRO_0000003568
Sites
	Metal binding	451	1	Iron (heme axial ligand)
	Binding site	315	1	Heme (covalent; via 1 link)

Sequences

	Sequence		Length	Mass (Da)
	P20816 [UniParc]. Last modified August 1, 1991. Version 2. Checksum: 8A795DD454125287	FASTA	504	57,969
	10 20 30 40 50 60 MGFSVFSPTR SLDGVSGFFQ GAFLLSLFLV LFKAVQFYLR RQWLLKALEK FPSTPSHWLW 70 80 90 100 110 120 GHNLKDREFQ QVLTWVEKFP GACLQWLSGS TARVLLYDPD YVKVVLGRSD PKPYQSLAPW 130 140 150 160 170 180 IGYGLLLLNG KKWFQHRRML TPAFHYDILK PYVKIMADSV SIMLDKWEKL DDQDHPLEIF 190 200 210 220 230 240 HYVSLMTLDT VMKCAFSHQG SVQLDVNSRS YTKAVEDLNN LIFFRVRSAF YGNSIIYNMS 250 260 270 280 290 300 SDGRLSRRAC QIAHEHTDGV IKTRKAQLQN EEELQKARKK RHLDFLDILL FAKMEDGKSL 310 320 330 340 350 360 SDEDLRAEVD TFMFEGHDTT ASGISWVFYA LATHPEHQER CREEVQSILG DGTSVTWDHL 370 380 390 400 410 420 DQMPYTTMCI KEALRLYSPV PSVSRELSSP VTFPDGRSIP KGIRVTILIY GLHHNPSYWP 430 440 450 460 470 480 NPKVFDPSRF SPDSPRHSHA YLPFSGGARN CIGKQFAMNE LKVAVALTLL RFELLPDPTR 490 500 IPVPMPRLVL KSKNGIHLRL KKLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The rat clofibrate-inducible CYP4A gene subfamily. I. Complete intron and exon sequence of the CYP4A1 and CYP4A2 genes, unique exon organization, and identification of a conserved 19-bp upstream element." Kimura S., Hanioka N., Matsunaga E., Gonzalez F.J. DNA 8:503-516(1989) [PubMed: 2766932] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Purification and NH2-terminal amino acid sequences of human and rat kidney fatty acid omega-hydroxylases." Kawashima H., Kusunose E., Kubota I., Maekawa M., Kusunose M. Biochim. Biophys. Acta 1123:156-162(1992) [PubMed: 1739747] [Abstract] Cited for: PROTEIN SEQUENCE OF 5-34. Tissue: Kidney.
[4]	"Characterization of three cytochrome P450s purified from renal microsomes of untreated male rats and comparison with human renal cytochrome P450." Imaoka S., Nagashima K., Funae Y. Arch. Biochem. Biophys. 276:473-480(1990) [PubMed: 2306108] [Abstract] Cited for: PROTEIN SEQUENCE OF 5-19. Strain: Sprague-Dawley. Tissue: Kidney.
[5]	"Covalently linked heme in cytochrome P4504A fatty acid hydroxylases." Hoch U., Ortiz de Montellano P.R. J. Biol. Chem. 276:11339-11346(2001) [PubMed: 11139583] [Abstract] Cited for: COVALENT HEME ATTACHMENT.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57719 Genomic DNA. Translation: AAA41039.1. BC078684 mRNA. Translation: AAH78684.1. BC098705 mRNA. Translation: AAH98705.1.
IPI	IPI00203317.
PIR	A32965. PC4350.
RefSeq	NP_001038235.1. NM_001044770.2.
UniGene	Rn.214991. Rn.33492.
3D structure databases
ProteinModelPortal	P20816.
ModBase	Search...
Protein-protein interaction databases
STRING	P20816.
Proteomic databases
PRIDE	P20816.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24306.
KEGG	rno:24306.
Organism-specific databases
CTD	24306.
RGD	2479. Cyp4a2.
Phylogenomic databases
eggNOG	maNOG05356.
HOVERGEN	HBG000182.
Gene expression databases
ArrayExpress	P20816.
Genevestigator	P20816.
GermOnline	ENSRNOG00000030154. Rattus norvegicus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
KO	K07425.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	602929.

Entry information

Entry name

CP4A2_RAT

Accession

Primary (citable) accession number: P20816
Secondary accession number(s): Q4G071

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	August 1, 1991
Last modified:	January 25, 2012
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program