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Protein

Cytochrome P450 4A2

Gene

Cyp4a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate, myristate and palmitate. Has little activity toward prostaglandins A1 and E1. Oxidizes arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE).1 Publication

Catalytic activityi

A long-chain fatty acid + NADPH + O2 = an omega-hydroxy-long-chain fatty acid + NADP+ + H2O.1 Publication

Cofactori

heme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei315 – 3151Heme (covalent; via 1 link)1 Publication
Metal bindingi451 – 4511Iron (heme axial ligand)1 Publication

GO - Molecular functioni

  • alkane 1-monooxygenase activity Source: RGD
  • arachidonic acid 11,12-epoxygenase activity Source: RGD
  • arachidonic acid binding Source: RGD
  • arachidonic acid monooxygenase activity Source: RGD
  • fatty acid binding Source: RGD
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

  • arachidonic acid metabolic process Source: RGD
  • icosanoid biosynthetic process Source: RGD
  • kidney development Source: RGD
  • lauric acid metabolic process Source: RGD
  • linoleic acid metabolic process Source: RGD
  • response to drug Source: RGD
  • response to hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-RNO-211935. Fatty acids.
R-RNO-2142816. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Chemistry

SwissLipidsiSLP:000000743.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4A2
Alternative name(s):
CYPIVA2
Cytochrome P-450 K-2
Cytochrome P450 K-5
Cytochrome P450-LA-omega 2
Lauric acid omega-hydroxylase
Long-chain fatty acid omega-monooxygenase (EC:1.14.13.205)
Gene namesi
Name:Cyp4a2
Synonyms:Cyp4a-2, Cyp4a11
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2479. Cyp4a2.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 442 PublicationsPRO_0000003567
Chaini5 – 504500Cytochrome P450 4A2PRO_0000003568Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei434 – 4341PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP20816.
PRIDEiP20816.

Expressioni

Inductioni

By clofibrate.

Gene expression databases

GenevisibleiP20816. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4996520.
STRINGi10116.ENSRNOP00000042072.

Structurei

3D structure databases

ProteinModelPortaliP20816.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOVERGENiHBG000182.
InParanoidiP20816.
KOiK07425.
OMAiKCRREAQ.
OrthoDBiEOG7CNZFK.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFSVFSPTR SLDGVSGFFQ GAFLLSLFLV LFKAVQFYLR RQWLLKALEK
60 70 80 90 100
FPSTPSHWLW GHNLKDREFQ QVLTWVEKFP GACLQWLSGS TARVLLYDPD
110 120 130 140 150
YVKVVLGRSD PKPYQSLAPW IGYGLLLLNG KKWFQHRRML TPAFHYDILK
160 170 180 190 200
PYVKIMADSV SIMLDKWEKL DDQDHPLEIF HYVSLMTLDT VMKCAFSHQG
210 220 230 240 250
SVQLDVNSRS YTKAVEDLNN LIFFRVRSAF YGNSIIYNMS SDGRLSRRAC
260 270 280 290 300
QIAHEHTDGV IKTRKAQLQN EEELQKARKK RHLDFLDILL FAKMEDGKSL
310 320 330 340 350
SDEDLRAEVD TFMFEGHDTT ASGISWVFYA LATHPEHQER CREEVQSILG
360 370 380 390 400
DGTSVTWDHL DQMPYTTMCI KEALRLYSPV PSVSRELSSP VTFPDGRSIP
410 420 430 440 450
KGIRVTILIY GLHHNPSYWP NPKVFDPSRF SPDSPRHSHA YLPFSGGARN
460 470 480 490 500
CIGKQFAMNE LKVAVALTLL RFELLPDPTR IPVPMPRLVL KSKNGIHLRL

KKLR
Length:504
Mass (Da):57,969
Last modified:August 1, 1991 - v2
Checksum:i8A795DD454125287
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57719 Genomic DNA. Translation: AAA41039.1.
BC078684 mRNA. Translation: AAH78684.1.
BC098705 mRNA. Translation: AAH98705.1.
PIRiA32965.
PC4350.
RefSeqiNP_001038235.1. NM_001044770.2.
UniGeneiRn.214991.

Genome annotation databases

EnsembliENSRNOT00000051252; ENSRNOP00000042072; ENSRNOG00000030154.
ENSRNOT00000083686; ENSRNOP00000072485; ENSRNOG00000030154.
ENSRNOT00000088209; ENSRNOP00000074484; ENSRNOG00000030154.
GeneIDi24306.
KEGGirno:24306.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57719 Genomic DNA. Translation: AAA41039.1.
BC078684 mRNA. Translation: AAH78684.1.
BC098705 mRNA. Translation: AAH98705.1.
PIRiA32965.
PC4350.
RefSeqiNP_001038235.1. NM_001044770.2.
UniGeneiRn.214991.

3D structure databases

ProteinModelPortaliP20816.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4996520.
STRINGi10116.ENSRNOP00000042072.

Chemistry

SwissLipidsiSLP:000000743.

Proteomic databases

PaxDbiP20816.
PRIDEiP20816.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000051252; ENSRNOP00000042072; ENSRNOG00000030154.
ENSRNOT00000083686; ENSRNOP00000072485; ENSRNOG00000030154.
ENSRNOT00000088209; ENSRNOP00000074484; ENSRNOG00000030154.
GeneIDi24306.
KEGGirno:24306.

Organism-specific databases

CTDi24306.
RGDi2479. Cyp4a2.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOVERGENiHBG000182.
InParanoidiP20816.
KOiK07425.
OMAiKCRREAQ.
OrthoDBiEOG7CNZFK.

Enzyme and pathway databases

ReactomeiR-RNO-211935. Fatty acids.
R-RNO-2142816. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Miscellaneous databases

NextBioi602929.
PROiP20816.

Gene expression databases

GenevisibleiP20816. RN.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The rat clofibrate-inducible CYP4A gene subfamily. I. Complete intron and exon sequence of the CYP4A1 and CYP4A2 genes, unique exon organization, and identification of a conserved 19-bp upstream element."
    Kimura S., Hanioka N., Matsunaga E., Gonzalez F.J.
    DNA 8:503-516(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. "Purification and NH2-terminal amino acid sequences of human and rat kidney fatty acid omega-hydroxylases."
    Kawashima H., Kusunose E., Kubota I., Maekawa M., Kusunose M.
    Biochim. Biophys. Acta 1123:156-162(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 5-34, FUNCTION, CATALYTIC ACTIVITY.
    Tissue: Kidney.
  4. "Characterization of three cytochrome P450s purified from renal microsomes of untreated male rats and comparison with human renal cytochrome P450."
    Imaoka S., Nagashima K., Funae Y.
    Arch. Biochem. Biophys. 276:473-480(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 5-19.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  5. "Covalently linked heme in cytochrome P4504A fatty acid hydroxylases."
    Hoch U., Ortiz de Montellano P.R.
    J. Biol. Chem. 276:11339-11346(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: COVALENT HEME ATTACHMENT.
  6. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-434, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCP4A2_RAT
AccessioniPrimary (citable) accession number: P20816
Secondary accession number(s): Q4G071
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: August 1, 1991
Last modified: February 17, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.