Cytochrome P450 2C13, male-specific - Rattus norvegicus (Rat)

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P20814 (CP2CD_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 106. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome P450 2C13, male-specific
EC=1.14.14.1

Alternative name(s):
CYPIIC13
Cytochrome P-450g
Cytochrome P450-G
Cytochrome P450-UT-5

Gene names

Name:	Cyp2c13
Synonyms:	Cyp2c-13

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Liver, and to a lesser extent in prostate, kidney, heart and brain.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Miscellaneous	The G^- form of this protein is thought to be unexpressed.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Traceable author statement Ref.2. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 490

490

Cytochrome P450 2C13, male-specific

PRO_0000051703

Sites

Metal binding

435

Iron (heme axial ligand)

Natural variations

Natural variant

P → L in G- phenotype.

Natural variant

180

S → C in G- phenotype.

Natural variant

234

F → L in G- phenotype.

Natural variant

237

H → Y in G- phenotype.

Natural variant

240

L → V in G- phenotype.

Natural variant

338

C → S in G- phenotype.

Natural variant

369

E → D in G- phenotype.

Experimental info

Sequence conflict

353

H → Q in AAA41059. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

P20814 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: F585891A7BB9F536
Show »

FASTA

490

55,860

        10         20         30         40         50         60 
MDPVVVLLLS LFFLLFLSLW RPSSGRGKLP PGPTPLPIIG NFFQVDMKDI RQSLTNFSKT 

        70         80         90        100        110        120 
YGPVYTLYVG SQPTVVLHGY EALKEALVDH GEEFSGRGRL PICEKVAKGQ GIAFSHGNVW 

       130        140        150        160        170        180 
KATRHFTVKT LRNLGMGKGT IEDKVQEEAK WLVKELKKTN GSPCDPQFIM GCAPGNVICS 

       190        200        210        220        230        240 
IILQNRFDYE DKDFLNLIEK VNEAVKIISS PGIQVFNIFP ILLDYCPGNH NIYFKNHTWL 

       250        260        270        280        290        300 
KSYLLEKIKE HEESLDVSNP RDFIDYFLIE RNQENANQWM NYTLEHLAIM VTDLFFAGIE 

       310        320        330        340        350        360 
TVSSTMRFAL LLLMKYPHVT AKVQEEIDHV IGRHRSPCMQ DRSHMPYTNA MVHEVQRYID 

       370        380        390        400        410        420 
IGPNGLLHEV TCDTKFRNYF IPKGTAVLTS LTSVLHDSKE FPNPEMFDPG HFLDENGNFK 

       430        440        450        460        470        480 
KSDYFIPFSA GKRMCLGESL ARMELFLFLT TILQNFKLKS LVDPKDINTT PICSSLSSVP 

       490 
PTFQMRFIPL

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References

[1]	"Characterization of a cDNA for rat P-450g, a highly polymorphic, male-specific cytochrome in the P-450IIC subfamily." McClellan-Green P.D., Negishi M., Goldstein J.A. Biochemistry 28:5832-5839(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (G+). Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Structural and regulatory analysis of the male-specific rat liver cytochrome P-450 g: repression by continuous growth hormone administration." Zaphiropoulos P.G., Stroem A., Robertson J.A., Gustafsson J.-A. Mol. Endocrinol. 4:53-58(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (G-). Strain: Sprague-Dawley. Tissue: Liver.
[3]	"Gene structure and expression of the rat cytochrome P450IIC13, a polymorphic male-specific cytochrome in the P450IIC subfamily." Eguchi H., Westin S., Stroem A., Gustafsson J.-A., Zaphiropolos P.G. Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Characterization of a cDNA for the unexpressed form of cytochrome P-450g from the (-g) rat and differentiation of its mRNA from that of the (+g) phenotype using specific oligoprobes." Yeowell H.N., McClellan-Green P.D., Negishi M., Goldstein J.A. Biochemistry 29:713-718(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-468 (G-). Strain: Sprague-Dawley. Tissue: Liver.
[5]	"Purification and characterization of three male-specific and one female-specific forms of cytochrome P-450 from rat liver microsomes." Matsumoto T., Emi Y., Kawabata S., Omura T. J. Biochem. 100:1359-1371(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-30 (G+).

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02861 mRNA. Translation: AAA41785.1. M33994 mRNA. Translation: AAA41063.1. M82855 M82852 Genomic DNA. Translation: AAA41059.1. M32277 mRNA. Translation: AAA41031.1.
IPI	IPI00325874.
PIR	A36122. I52410.
RefSeq	NP_612523.1. NM_138514.1.
UniGene	Rn.82715.
3D structure databases
ProteinModelPortal	P20814.
SMR	P20814. Positions 30-490.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4564628.
PTM databases
PhosphoSite	P20814.
Proteomic databases
PRIDE	P20814.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	171521.
KEGG	rno:171521.
Organism-specific databases
CTD	171521.
RGD	620363. Cyp2c13.
Phylogenomic databases
HOVERGEN	HBG015789.
KO	K07413.
Gene expression databases
Genevestigator	P20814.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	622497.

Entry information

Entry name

CP2CD_RAT

Accession

Primary (citable) accession number: P20814
Secondary accession number(s): P22693, Q64587

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 29, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents