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P20814 (CP2CD_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2C13, male-specific

EC=1.14.14.1
Alternative name(s):
CYPIIC13
Cytochrome P-450g
Cytochrome P450-G
Cytochrome P450-UT-5
Gene names
Name:Cyp2c13
Synonyms:Cyp2c-13
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Liver, and to a lesser extent in prostate, kidney, heart and brain.

Induction

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Miscellaneous

The G- form of this protein is thought to be unexpressed.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Cytochrome P450 2C13, male-specific
PRO_0000051703

Sites

Metal binding4351Iron (heme axial ligand)

Natural variations

Natural variant221P → L in G- phenotype.
Natural variant1801S → C in G- phenotype.
Natural variant2341F → L in G- phenotype.
Natural variant2371H → Y in G- phenotype.
Natural variant2401L → V in G- phenotype.
Natural variant3381C → S in G- phenotype.
Natural variant3691E → D in G- phenotype.

Experimental info

Sequence conflict3531H → Q in AAA41059. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P20814 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: F585891A7BB9F536

FASTA49055,860
        10         20         30         40         50         60 
MDPVVVLLLS LFFLLFLSLW RPSSGRGKLP PGPTPLPIIG NFFQVDMKDI RQSLTNFSKT 

        70         80         90        100        110        120 
YGPVYTLYVG SQPTVVLHGY EALKEALVDH GEEFSGRGRL PICEKVAKGQ GIAFSHGNVW 

       130        140        150        160        170        180 
KATRHFTVKT LRNLGMGKGT IEDKVQEEAK WLVKELKKTN GSPCDPQFIM GCAPGNVICS 

       190        200        210        220        230        240 
IILQNRFDYE DKDFLNLIEK VNEAVKIISS PGIQVFNIFP ILLDYCPGNH NIYFKNHTWL 

       250        260        270        280        290        300 
KSYLLEKIKE HEESLDVSNP RDFIDYFLIE RNQENANQWM NYTLEHLAIM VTDLFFAGIE 

       310        320        330        340        350        360 
TVSSTMRFAL LLLMKYPHVT AKVQEEIDHV IGRHRSPCMQ DRSHMPYTNA MVHEVQRYID 

       370        380        390        400        410        420 
IGPNGLLHEV TCDTKFRNYF IPKGTAVLTS LTSVLHDSKE FPNPEMFDPG HFLDENGNFK 

       430        440        450        460        470        480 
KSDYFIPFSA GKRMCLGESL ARMELFLFLT TILQNFKLKS LVDPKDINTT PICSSLSSVP 

       490 
PTFQMRFIPL 

« Hide

References

[1]"Characterization of a cDNA for rat P-450g, a highly polymorphic, male-specific cytochrome in the P-450IIC subfamily."
McClellan-Green P.D., Negishi M., Goldstein J.A.
Biochemistry 28:5832-5839(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (G+).
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"Structural and regulatory analysis of the male-specific rat liver cytochrome P-450 g: repression by continuous growth hormone administration."
Zaphiropoulos P.G., Stroem A., Robertson J.A., Gustafsson J.-A.
Mol. Endocrinol. 4:53-58(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (G-).
Strain: Sprague-Dawley.
Tissue: Liver.
[3]"Gene structure and expression of the rat cytochrome P450IIC13, a polymorphic male-specific cytochrome in the P450IIC subfamily."
Eguchi H., Westin S., Stroem A., Gustafsson J.-A., Zaphiropolos P.G.
Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Characterization of a cDNA for the unexpressed form of cytochrome P-450g from the (-g) rat and differentiation of its mRNA from that of the (+g) phenotype using specific oligoprobes."
Yeowell H.N., McClellan-Green P.D., Negishi M., Goldstein J.A.
Biochemistry 29:713-718(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-468 (G-).
Strain: Sprague-Dawley.
Tissue: Liver.
[5]"Purification and characterization of three male-specific and one female-specific forms of cytochrome P-450 from rat liver microsomes."
Matsumoto T., Emi Y., Kawabata S., Omura T.
J. Biochem. 100:1359-1371(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-30 (G+).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02861 mRNA. Translation: AAA41785.1.
M33994 mRNA. Translation: AAA41063.1.
M82855 expand/collapse EMBL AC list , M82849, M82850, M82846, M82848, M82853, M82851, M82852 Genomic DNA. Translation: AAA41059.1.
M32277 mRNA. Translation: AAA41031.1.
PIRA36122.
I52410.
RefSeqNP_612523.1. NM_138514.1.
UniGeneRn.82715.

3D structure databases

ProteinModelPortalP20814.
SMRP20814. Positions 30-490.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4564628.

PTM databases

PhosphoSiteP20814.

Proteomic databases

PRIDEP20814.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID171521.
KEGGrno:171521.

Organism-specific databases

CTD171521.
RGD620363. Cyp2c13.

Phylogenomic databases

HOVERGENHBG015789.
KOK07413.
PhylomeDBP20814.

Gene expression databases

GenevestigatorP20814.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio622497.

Entry information

Entry nameCP2CD_RAT
AccessionPrimary (citable) accession number: P20814
Secondary accession number(s): P22693, Q64587
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families