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P20814

- CP2CD_RAT

UniProt

P20814 - CP2CD_RAT

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Protein

Cytochrome P450 2C13, male-specific

Gene
Cyp2c13, Cyp2c-13
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi435 – 4351Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. monooxygenase activity Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2C13, male-specific (EC:1.14.14.1)
Alternative name(s):
CYPIIC13
Cytochrome P-450g
Cytochrome P450-G
Cytochrome P450-UT-5
Gene namesi
Name:Cyp2c13
Synonyms:Cyp2c-13
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620363. Cyp2c13.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Cytochrome P450 2C13, male-specificPRO_0000051703Add
BLAST

Proteomic databases

PRIDEiP20814.

PTM databases

PhosphoSiteiP20814.

Expressioni

Tissue specificityi

Liver, and to a lesser extent in prostate, kidney, heart and brain.

Inductioni

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Gene expression databases

GenevestigatoriP20814.

Interactioni

Protein-protein interaction databases

MINTiMINT-4564628.

Structurei

3D structure databases

ProteinModelPortaliP20814.
SMRiP20814. Positions 30-490.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Phylogenomic databases

HOVERGENiHBG015789.
KOiK07413.
PhylomeDBiP20814.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20814-1 [UniParc]FASTAAdd to Basket

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MDPVVVLLLS LFFLLFLSLW RPSSGRGKLP PGPTPLPIIG NFFQVDMKDI    50
RQSLTNFSKT YGPVYTLYVG SQPTVVLHGY EALKEALVDH GEEFSGRGRL 100
PICEKVAKGQ GIAFSHGNVW KATRHFTVKT LRNLGMGKGT IEDKVQEEAK 150
WLVKELKKTN GSPCDPQFIM GCAPGNVICS IILQNRFDYE DKDFLNLIEK 200
VNEAVKIISS PGIQVFNIFP ILLDYCPGNH NIYFKNHTWL KSYLLEKIKE 250
HEESLDVSNP RDFIDYFLIE RNQENANQWM NYTLEHLAIM VTDLFFAGIE 300
TVSSTMRFAL LLLMKYPHVT AKVQEEIDHV IGRHRSPCMQ DRSHMPYTNA 350
MVHEVQRYID IGPNGLLHEV TCDTKFRNYF IPKGTAVLTS LTSVLHDSKE 400
FPNPEMFDPG HFLDENGNFK KSDYFIPFSA GKRMCLGESL ARMELFLFLT 450
TILQNFKLKS LVDPKDINTT PICSSLSSVP PTFQMRFIPL 490
Length:490
Mass (Da):55,860
Last modified:February 1, 1991 - v1
Checksum:iF585891A7BB9F536
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti22 – 221P → L in G- phenotype.
Natural varianti180 – 1801S → C in G- phenotype.
Natural varianti234 – 2341F → L in G- phenotype.
Natural varianti237 – 2371H → Y in G- phenotype.
Natural varianti240 – 2401L → V in G- phenotype.
Natural varianti338 – 3381C → S in G- phenotype.
Natural varianti369 – 3691E → D in G- phenotype.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti353 – 3531H → Q in AAA41059. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02861 mRNA. Translation: AAA41785.1.
M33994 mRNA. Translation: AAA41063.1.
M82855
, M82849, M82850, M82846, M82848, M82853, M82851, M82852 Genomic DNA. Translation: AAA41059.1.
M32277 mRNA. Translation: AAA41031.1.
PIRiA36122.
I52410.
RefSeqiNP_612523.1. NM_138514.1.
UniGeneiRn.82715.

Genome annotation databases

GeneIDi171521.
KEGGirno:171521.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02861 mRNA. Translation: AAA41785.1 .
M33994 mRNA. Translation: AAA41063.1 .
M82855
, M82849 , M82850 , M82846 , M82848 , M82853 , M82851 , M82852 Genomic DNA. Translation: AAA41059.1 .
M32277 mRNA. Translation: AAA41031.1 .
PIRi A36122.
I52410.
RefSeqi NP_612523.1. NM_138514.1.
UniGenei Rn.82715.

3D structure databases

ProteinModelPortali P20814.
SMRi P20814. Positions 30-490.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4564628.

PTM databases

PhosphoSitei P20814.

Proteomic databases

PRIDEi P20814.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 171521.
KEGGi rno:171521.

Organism-specific databases

CTDi 171521.
RGDi 620363. Cyp2c13.

Phylogenomic databases

HOVERGENi HBG015789.
KOi K07413.
PhylomeDBi P20814.

Miscellaneous databases

NextBioi 622497.

Gene expression databases

Genevestigatori P20814.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of a cDNA for rat P-450g, a highly polymorphic, male-specific cytochrome in the P-450IIC subfamily."
    McClellan-Green P.D., Negishi M., Goldstein J.A.
    Biochemistry 28:5832-5839(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (G+).
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Structural and regulatory analysis of the male-specific rat liver cytochrome P-450 g: repression by continuous growth hormone administration."
    Zaphiropoulos P.G., Stroem A., Robertson J.A., Gustafsson J.-A.
    Mol. Endocrinol. 4:53-58(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (G-).
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "Gene structure and expression of the rat cytochrome P450IIC13, a polymorphic male-specific cytochrome in the P450IIC subfamily."
    Eguchi H., Westin S., Stroem A., Gustafsson J.-A., Zaphiropolos P.G.
    Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Characterization of a cDNA for the unexpressed form of cytochrome P-450g from the (-g) rat and differentiation of its mRNA from that of the (+g) phenotype using specific oligoprobes."
    Yeowell H.N., McClellan-Green P.D., Negishi M., Goldstein J.A.
    Biochemistry 29:713-718(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-468 (G-).
    Strain: Sprague-Dawley.
    Tissue: Liver.
  5. "Purification and characterization of three male-specific and one female-specific forms of cytochrome P-450 from rat liver microsomes."
    Matsumoto T., Emi Y., Kawabata S., Omura T.
    J. Biochem. 100:1359-1371(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-30 (G+).

Entry informationi

Entry nameiCP2CD_RAT
AccessioniPrimary (citable) accession number: P20814
Secondary accession number(s): P22693, Q64587
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The G- form of this protein is thought to be unexpressed.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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