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P20814

- CP2CD_RAT

UniProt

P20814 - CP2CD_RAT

Protein

Cytochrome P450 2C13, male-specific

Gene

Cyp2c13

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi435 – 4351Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. monooxygenase activity Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2C13, male-specific (EC:1.14.14.1)
    Alternative name(s):
    CYPIIC13
    Cytochrome P-450g
    Cytochrome P450-G
    Cytochrome P450-UT-5
    Gene namesi
    Name:Cyp2c13
    Synonyms:Cyp2c-13
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620363. Cyp2c13.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Cytochrome P450 2C13, male-specificPRO_0000051703Add
    BLAST

    Proteomic databases

    PRIDEiP20814.

    PTM databases

    PhosphoSiteiP20814.

    Expressioni

    Tissue specificityi

    Liver, and to a lesser extent in prostate, kidney, heart and brain.

    Inductioni

    P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

    Gene expression databases

    GenevestigatoriP20814.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4564628.

    Structurei

    3D structure databases

    ProteinModelPortaliP20814.
    SMRiP20814. Positions 30-490.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    HOVERGENiHBG015789.
    KOiK07413.
    PhylomeDBiP20814.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20814-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPVVVLLLS LFFLLFLSLW RPSSGRGKLP PGPTPLPIIG NFFQVDMKDI    50
    RQSLTNFSKT YGPVYTLYVG SQPTVVLHGY EALKEALVDH GEEFSGRGRL 100
    PICEKVAKGQ GIAFSHGNVW KATRHFTVKT LRNLGMGKGT IEDKVQEEAK 150
    WLVKELKKTN GSPCDPQFIM GCAPGNVICS IILQNRFDYE DKDFLNLIEK 200
    VNEAVKIISS PGIQVFNIFP ILLDYCPGNH NIYFKNHTWL KSYLLEKIKE 250
    HEESLDVSNP RDFIDYFLIE RNQENANQWM NYTLEHLAIM VTDLFFAGIE 300
    TVSSTMRFAL LLLMKYPHVT AKVQEEIDHV IGRHRSPCMQ DRSHMPYTNA 350
    MVHEVQRYID IGPNGLLHEV TCDTKFRNYF IPKGTAVLTS LTSVLHDSKE 400
    FPNPEMFDPG HFLDENGNFK KSDYFIPFSA GKRMCLGESL ARMELFLFLT 450
    TILQNFKLKS LVDPKDINTT PICSSLSSVP PTFQMRFIPL 490
    Length:490
    Mass (Da):55,860
    Last modified:February 1, 1991 - v1
    Checksum:iF585891A7BB9F536
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti353 – 3531H → Q in AAA41059. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti22 – 221P → L in G- phenotype.
    Natural varianti180 – 1801S → C in G- phenotype.
    Natural varianti234 – 2341F → L in G- phenotype.
    Natural varianti237 – 2371H → Y in G- phenotype.
    Natural varianti240 – 2401L → V in G- phenotype.
    Natural varianti338 – 3381C → S in G- phenotype.
    Natural varianti369 – 3691E → D in G- phenotype.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02861 mRNA. Translation: AAA41785.1.
    M33994 mRNA. Translation: AAA41063.1.
    M82855
    , M82849, M82850, M82846, M82848, M82853, M82851, M82852 Genomic DNA. Translation: AAA41059.1.
    M32277 mRNA. Translation: AAA41031.1.
    PIRiA36122.
    I52410.
    RefSeqiNP_612523.1. NM_138514.1.
    UniGeneiRn.82715.

    Genome annotation databases

    GeneIDi171521.
    KEGGirno:171521.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02861 mRNA. Translation: AAA41785.1 .
    M33994 mRNA. Translation: AAA41063.1 .
    M82855
    , M82849 , M82850 , M82846 , M82848 , M82853 , M82851 , M82852 Genomic DNA. Translation: AAA41059.1 .
    M32277 mRNA. Translation: AAA41031.1 .
    PIRi A36122.
    I52410.
    RefSeqi NP_612523.1. NM_138514.1.
    UniGenei Rn.82715.

    3D structure databases

    ProteinModelPortali P20814.
    SMRi P20814. Positions 30-490.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4564628.

    PTM databases

    PhosphoSitei P20814.

    Proteomic databases

    PRIDEi P20814.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 171521.
    KEGGi rno:171521.

    Organism-specific databases

    CTDi 171521.
    RGDi 620363. Cyp2c13.

    Phylogenomic databases

    HOVERGENi HBG015789.
    KOi K07413.
    PhylomeDBi P20814.

    Miscellaneous databases

    NextBioi 622497.

    Gene expression databases

    Genevestigatori P20814.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a cDNA for rat P-450g, a highly polymorphic, male-specific cytochrome in the P-450IIC subfamily."
      McClellan-Green P.D., Negishi M., Goldstein J.A.
      Biochemistry 28:5832-5839(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (G+).
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "Structural and regulatory analysis of the male-specific rat liver cytochrome P-450 g: repression by continuous growth hormone administration."
      Zaphiropoulos P.G., Stroem A., Robertson J.A., Gustafsson J.-A.
      Mol. Endocrinol. 4:53-58(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (G-).
      Strain: Sprague-Dawley.
      Tissue: Liver.
    3. "Gene structure and expression of the rat cytochrome P450IIC13, a polymorphic male-specific cytochrome in the P450IIC subfamily."
      Eguchi H., Westin S., Stroem A., Gustafsson J.-A., Zaphiropolos P.G.
      Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Characterization of a cDNA for the unexpressed form of cytochrome P-450g from the (-g) rat and differentiation of its mRNA from that of the (+g) phenotype using specific oligoprobes."
      Yeowell H.N., McClellan-Green P.D., Negishi M., Goldstein J.A.
      Biochemistry 29:713-718(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-468 (G-).
      Strain: Sprague-Dawley.
      Tissue: Liver.
    5. "Purification and characterization of three male-specific and one female-specific forms of cytochrome P-450 from rat liver microsomes."
      Matsumoto T., Emi Y., Kawabata S., Omura T.
      J. Biochem. 100:1359-1371(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-30 (G+).

    Entry informationi

    Entry nameiCP2CD_RAT
    AccessioniPrimary (citable) accession number: P20814
    Secondary accession number(s): P22693, Q64587
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The G- form of this protein is thought to be unexpressed.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3