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P20812

- CP2A3_RAT

UniProt

P20812 - CP2A3_RAT

Protein

Cytochrome P450 2A3

Gene

Cyp2a3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi439 – 4391Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. monooxygenase activity Source: RGD
    5. oxygen binding Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2A3 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIA3
    Coumarin 7-hydroxylase
    Gene namesi
    Name:Cyp2a3
    Synonyms:Cyp2a-3, Cyp2a3a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2465. Cyp2a3.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Cytochrome P450 2A3PRO_0000051665Add
    BLAST

    Proteomic databases

    PaxDbiP20812.
    PRIDEiP20812.

    PTM databases

    PhosphoSiteiP20812.

    Expressioni

    Tissue specificityi

    Lung.

    Inductioni

    By 3-methylcholanthrene (3MC).

    Gene expression databases

    GenevestigatoriP20812.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000054077.

    Structurei

    3D structure databases

    ProteinModelPortaliP20812.
    SMRiP20812. Positions 31-494.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.
    KOiK07411.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20812-1 [UniParc]FASTAAdd to Basket

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    MLASGLLLVA SVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFIGNYLQLN    50
    TEKMYSSLMK ISQRYGPVFT IHLGPRRVVV LCGQEAVKEA LVDQAEEFSG 100
    RGEQATFDWL FKGYGVAFSS GERAKQLRRF SIATLRDFGV GKRGIEERIQ 150
    EEAGFLIESF RKTNGALIDP TFYLSRTVSN VISSIVFGDR FDYEDKEFLS 200
    LLRMMLGSFQ FTATSTGQLY EMFSSVMKHL PGPQQQAFKE LQGLEDFITK 250
    KVEQNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF 300
    AGTETVSTTL RYGFLLLMKH PDIEAKVHEE IDRVIGRNRQ AKYEDRMKMP 350
    YTEAVIHEIQ RFADMIPMGL ARRVTKDTKF REFLLPKGTE VFPMLGSVLK 400
    DPKFFSNPND FNPKHFLDDK GQFKKSDAFV PFSIGKRYCF GEGLARMELF 450
    LFLTNIMQNF CFKSPQAPQD IDVSPRLVGF ATIPPNYTMS FLSR 494
    Length:494
    Mass (Da):56,510
    Last modified:February 1, 1991 - v1
    Checksum:iF71855CBE602672F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti109 – 1091W → G in AAA88511. (PubMed:2751996)Curated
    Sequence conflicti335 – 3351I → N in AAA88511. (PubMed:2751996)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33190 Genomic DNA. Translation: AAA41022.1.
    J02852 mRNA. Translation: AAA88511.1.
    PIRiS15056. A32030.
    RefSeqiNP_036674.1. NM_012542.2.
    UniGeneiRn.2063.

    Genome annotation databases

    GeneIDi24299.
    KEGGirno:24299.
    UCSCiRGD:2465. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33190 Genomic DNA. Translation: AAA41022.1 .
    J02852 mRNA. Translation: AAA88511.1 .
    PIRi S15056. A32030.
    RefSeqi NP_036674.1. NM_012542.2.
    UniGenei Rn.2063.

    3D structure databases

    ProteinModelPortali P20812.
    SMRi P20812. Positions 31-494.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000054077.

    PTM databases

    PhosphoSitei P20812.

    Proteomic databases

    PaxDbi P20812.
    PRIDEi P20812.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24299.
    KEGGi rno:24299.
    UCSCi RGD:2465. rat.

    Organism-specific databases

    CTDi 24299.
    RGDi 2465. Cyp2a3.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.
    KOi K07411.

    Miscellaneous databases

    NextBioi 602916.

    Gene expression databases

    Genevestigatori P20812.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of the rat CYP2A3 gene specifically transcribed in lung."
      Ueno T., Gonzalez F.
      Nucleic Acids Res. 18:4623-4624(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
      Tissue: Lung.
    2. "Identification of a novel P450 expressed in rat lung: cDNA cloning and sequence, chromosome mapping, and induction by 3-methylcholanthrene."
      Kimura S., Kozak C.A., Gonzalez F.J.
      Biochemistry 28:3798-3803(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-494.
      Tissue: Lung.

    Entry informationi

    Entry nameiCP2A3_RAT
    AccessioniPrimary (citable) accession number: P20812
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3