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Protein

Cytochrome P450 2A3

Gene

Cyp2a3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi439 – 4391Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2A3 (EC:1.14.14.1)
Alternative name(s):
CYPIIA3
Coumarin 7-hydroxylase
Gene namesi
Name:Cyp2a3
Synonyms:Cyp2a-3, Cyp2a3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2465. Cyp2a3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Cytochrome P450 2A3PRO_0000051665Add
BLAST

Proteomic databases

PaxDbiP20812.
PRIDEiP20812.

PTM databases

PhosphoSiteiP20812.

Expressioni

Tissue specificityi

Lung.

Inductioni

By 3-methylcholanthrene (3MC).

Gene expression databases

GenevisibleiP20812. RN.

Structurei

3D structure databases

ProteinModelPortaliP20812.
SMRiP20812. Positions 31-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP20812.
KOiK07411.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLASGLLLVA SVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFIGNYLQLN
60 70 80 90 100
TEKMYSSLMK ISQRYGPVFT IHLGPRRVVV LCGQEAVKEA LVDQAEEFSG
110 120 130 140 150
RGEQATFDWL FKGYGVAFSS GERAKQLRRF SIATLRDFGV GKRGIEERIQ
160 170 180 190 200
EEAGFLIESF RKTNGALIDP TFYLSRTVSN VISSIVFGDR FDYEDKEFLS
210 220 230 240 250
LLRMMLGSFQ FTATSTGQLY EMFSSVMKHL PGPQQQAFKE LQGLEDFITK
260 270 280 290 300
KVEQNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF
310 320 330 340 350
AGTETVSTTL RYGFLLLMKH PDIEAKVHEE IDRVIGRNRQ AKYEDRMKMP
360 370 380 390 400
YTEAVIHEIQ RFADMIPMGL ARRVTKDTKF REFLLPKGTE VFPMLGSVLK
410 420 430 440 450
DPKFFSNPND FNPKHFLDDK GQFKKSDAFV PFSIGKRYCF GEGLARMELF
460 470 480 490
LFLTNIMQNF CFKSPQAPQD IDVSPRLVGF ATIPPNYTMS FLSR
Length:494
Mass (Da):56,510
Last modified:February 1, 1991 - v1
Checksum:iF71855CBE602672F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091W → G in AAA88511 (PubMed:2751996).Curated
Sequence conflicti335 – 3351I → N in AAA88511 (PubMed:2751996).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33190 Genomic DNA. Translation: AAA41022.1.
J02852 mRNA. Translation: AAA88511.1.
PIRiS15056. A32030.
RefSeqiNP_036674.1. NM_012542.2.
UniGeneiRn.2063.

Genome annotation databases

GeneIDi24299.
KEGGirno:24299.
UCSCiRGD:2465. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33190 Genomic DNA. Translation: AAA41022.1.
J02852 mRNA. Translation: AAA88511.1.
PIRiS15056. A32030.
RefSeqiNP_036674.1. NM_012542.2.
UniGeneiRn.2063.

3D structure databases

ProteinModelPortaliP20812.
SMRiP20812. Positions 31-494.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP20812.

Proteomic databases

PaxDbiP20812.
PRIDEiP20812.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24299.
KEGGirno:24299.
UCSCiRGD:2465. rat.

Organism-specific databases

CTDi24299.
RGDi2465. Cyp2a3.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP20812.
KOiK07411.

Miscellaneous databases

NextBioi602916.
PROiP20812.

Gene expression databases

GenevisibleiP20812. RN.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of the rat CYP2A3 gene specifically transcribed in lung."
    Ueno T., Gonzalez F.
    Nucleic Acids Res. 18:4623-4624(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
    Tissue: Lung.
  2. "Identification of a novel P450 expressed in rat lung: cDNA cloning and sequence, chromosome mapping, and induction by 3-methylcholanthrene."
    Kimura S., Kozak C.A., Gonzalez F.J.
    Biochemistry 28:3798-3803(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-494.
    Tissue: Lung.

Entry informationi

Entry nameiCP2A3_RAT
AccessioniPrimary (citable) accession number: P20812
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 22, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.