Reviewed, UniProtKB/Swiss-Prot P20812 (CP2A3_RAT)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 2A3
    EC=1.14.14.1
Alternative name(s):
    CYPIIA3
    Coumarin 7-hydroxylase

Gene names

Name:	Cyp2a3
Synonyms:	Cyp2a-3, Cyp2a3a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Lung.
Induction	By 3-methylcholanthrene (3MC).
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro oxygen binding Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Cytochrome P450 2A3		PRO_0000051665
Sites
	Metal binding	439	1	Iron (heme axial ligand)
Experimental info
	Sequence conflict	109	1	W → G in AAA88511. Ref.2
	Sequence conflict	335	1	I → N in AAA88511. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P20812-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: F71855CBE602672F	FASTA	494	56,510
	10 20 30 40 50 60 MLASGLLLVA SVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFIGNYLQLN TEKMYSSLMK 70 80 90 100 110 120 ISQRYGPVFT IHLGPRRVVV LCGQEAVKEA LVDQAEEFSG RGEQATFDWL FKGYGVAFSS 130 140 150 160 170 180 GERAKQLRRF SIATLRDFGV GKRGIEERIQ EEAGFLIESF RKTNGALIDP TFYLSRTVSN 190 200 210 220 230 240 VISSIVFGDR FDYEDKEFLS LLRMMLGSFQ FTATSTGQLY EMFSSVMKHL PGPQQQAFKE 250 260 270 280 290 300 LQGLEDFITK KVEQNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF 310 320 330 340 350 360 AGTETVSTTL RYGFLLLMKH PDIEAKVHEE IDRVIGRNRQ AKYEDRMKMP YTEAVIHEIQ 370 380 390 400 410 420 RFADMIPMGL ARRVTKDTKF REFLLPKGTE VFPMLGSVLK DPKFFSNPND FNPKHFLDDK 430 440 450 460 470 480 GQFKKSDAFV PFSIGKRYCF GEGLARMELF LFLTNIMQNF CFKSPQAPQD IDVSPRLVGF 490 ATIPPNYTMS FLSR

References

[1]	"Complete sequence of the rat CYP2A3 gene specifically transcribed in lung." Ueno T., Gonzalez F. Nucleic Acids Res. 18:4623-4624(1990) [PubMed: 2388852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Lung.
[2]	"Identification of a novel P450 expressed in rat lung: cDNA cloning and sequence, chromosome mapping, and induction by 3-methylcholanthrene." Kimura S., Kozak C.A., Gonzalez F.J. Biochemistry 28:3798-3803(1989) [PubMed: 2751996] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-494. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M33190 Genomic DNA. Translation: AAA41022.1. J02852 mRNA. Translation: AAA88511.1.
IPI	IPI00203312.
PIR	A32030. S15056.
RefSeq	NP_036674.1.
UniGene	Rn.2063
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
SMR	P20812. Positions 31-494.
ModBase	Search...
Protein-protein interaction databases
STRING	P20812.
Proteomic databases
PRIDE	P20812.
Genome annotation databases
GeneID	24299.
KEGG	rno:24299.
Organism-specific databases
CTD	24299.
RGD	2465. Cyp2a3a.
Phylogenomic databases
HOVERGEN	P20812.
Enzyme and pathway databases
BRENDA	1.14.14.1. 248.
Gene expression databases
Genevestigator	P20812.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008067. Cyt_P450_E_grp-I_CYP2A-like. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01684. EP450ICYP2A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	602916.

Entry information

Entry name

CP2A3_RAT

Accession

Primary (citable) accession number: P20812

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	October 13, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)