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P20812 (CP2A3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2A3

EC=1.14.14.1
Alternative name(s):
CYPIIA3
Coumarin 7-hydroxylase
Gene names
Name:Cyp2a3
Synonyms:Cyp2a-3, Cyp2a3a
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Lung.

Induction

By 3-methylcholanthrene (3MC).

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Cytochrome P450 2A3
PRO_0000051665

Sites

Metal binding4391Iron (heme axial ligand)

Experimental info

Sequence conflict1091W → G in AAA88511. Ref.2
Sequence conflict3351I → N in AAA88511. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P20812 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: F71855CBE602672F

FASTA49456,510
        10         20         30         40         50         60 
MLASGLLLVA SVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFIGNYLQLN TEKMYSSLMK 

        70         80         90        100        110        120 
ISQRYGPVFT IHLGPRRVVV LCGQEAVKEA LVDQAEEFSG RGEQATFDWL FKGYGVAFSS 

       130        140        150        160        170        180 
GERAKQLRRF SIATLRDFGV GKRGIEERIQ EEAGFLIESF RKTNGALIDP TFYLSRTVSN 

       190        200        210        220        230        240 
VISSIVFGDR FDYEDKEFLS LLRMMLGSFQ FTATSTGQLY EMFSSVMKHL PGPQQQAFKE 

       250        260        270        280        290        300 
LQGLEDFITK KVEQNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF 

       310        320        330        340        350        360 
AGTETVSTTL RYGFLLLMKH PDIEAKVHEE IDRVIGRNRQ AKYEDRMKMP YTEAVIHEIQ 

       370        380        390        400        410        420 
RFADMIPMGL ARRVTKDTKF REFLLPKGTE VFPMLGSVLK DPKFFSNPND FNPKHFLDDK 

       430        440        450        460        470        480 
GQFKKSDAFV PFSIGKRYCF GEGLARMELF LFLTNIMQNF CFKSPQAPQD IDVSPRLVGF 

       490 
ATIPPNYTMS FLSR 

« Hide

References

[1]"Complete sequence of the rat CYP2A3 gene specifically transcribed in lung."
Ueno T., Gonzalez F.
Nucleic Acids Res. 18:4623-4624(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
Tissue: Lung.
[2]"Identification of a novel P450 expressed in rat lung: cDNA cloning and sequence, chromosome mapping, and induction by 3-methylcholanthrene."
Kimura S., Kozak C.A., Gonzalez F.J.
Biochemistry 28:3798-3803(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-494.
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M33190 Genomic DNA. Translation: AAA41022.1.
J02852 mRNA. Translation: AAA88511.1.
PIRA32030. S15056.
RefSeqNP_036674.1. NM_012542.2.
UniGeneRn.2063.

3D structure databases

ProteinModelPortalP20812.
SMRP20812. Positions 31-494.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000054077.

PTM databases

PhosphoSiteP20812.

Proteomic databases

PaxDbP20812.
PRIDEP20812.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24299.
KEGGrno:24299.
UCSCRGD:2465. rat.

Organism-specific databases

CTD24299.
RGD2465. Cyp2a3.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036992.
HOVERGENHBG015789.
KOK07411.

Gene expression databases

GenevestigatorP20812.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio602916.

Entry information

Entry nameCP2A3_RAT
AccessionPrimary (citable) accession number: P20812
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: January 22, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families