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Protein

Cytochrome P450 2A3

Gene

Cyp2a3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi439Iron (heme axial ligand)1

GO - Molecular functioni

  • arachidonic acid epoxygenase activity Source: GO_Central
  • aromatase activity Source: UniProtKB-EC
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • monooxygenase activity Source: RGD
  • oxygen binding Source: RGD
  • steroid hydroxylase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2A3 (EC:1.14.14.1)
Alternative name(s):
CYPIIA3
Coumarin 7-hydroxylase
Gene namesi
Name:Cyp2a3
Synonyms:Cyp2a-3, Cyp2a3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2465. Cyp2a3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000516651 – 494Cytochrome P450 2A3Add BLAST494

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei131PhosphoserineBy similarity1
Modified residuei379N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP20812.

PTM databases

PhosphoSitePlusiP20812.

Expressioni

Tissue specificityi

Lung.

Inductioni

By 3-methylcholanthrene (3MC).

Structurei

3D structure databases

ProteinModelPortaliP20812.
SMRiP20812.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP20812.
KOiK07411.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
IPR036396. Cyt_P450_sf.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

P20812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLASGLLLVA SVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFIGNYLQLN
60 70 80 90 100
TEKMYSSLMK ISQRYGPVFT IHLGPRRVVV LCGQEAVKEA LVDQAEEFSG
110 120 130 140 150
RGEQATFDWL FKGYGVAFSS GERAKQLRRF SIATLRDFGV GKRGIEERIQ
160 170 180 190 200
EEAGFLIESF RKTNGALIDP TFYLSRTVSN VISSIVFGDR FDYEDKEFLS
210 220 230 240 250
LLRMMLGSFQ FTATSTGQLY EMFSSVMKHL PGPQQQAFKE LQGLEDFITK
260 270 280 290 300
KVEQNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF
310 320 330 340 350
AGTETVSTTL RYGFLLLMKH PDIEAKVHEE IDRVIGRNRQ AKYEDRMKMP
360 370 380 390 400
YTEAVIHEIQ RFADMIPMGL ARRVTKDTKF REFLLPKGTE VFPMLGSVLK
410 420 430 440 450
DPKFFSNPND FNPKHFLDDK GQFKKSDAFV PFSIGKRYCF GEGLARMELF
460 470 480 490
LFLTNIMQNF CFKSPQAPQD IDVSPRLVGF ATIPPNYTMS FLSR
Length:494
Mass (Da):56,510
Last modified:February 1, 1991 - v1
Checksum:iF71855CBE602672F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109W → G in AAA88511 (PubMed:2751996).Curated1
Sequence conflicti335I → N in AAA88511 (PubMed:2751996).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33190 Genomic DNA. Translation: AAA41022.1.
J02852 mRNA. Translation: AAA88511.1.
PIRiS15056. A32030.
RefSeqiNP_036674.1. NM_012542.2.
UniGeneiRn.2063.

Genome annotation databases

GeneIDi24299.
KEGGirno:24299.
UCSCiRGD:2465. rat.

Similar proteinsi

Entry informationi

Entry nameiCP2A3_RAT
AccessioniPrimary (citable) accession number: P20812
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 22, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families