P20810 (ICAL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calpastatin

Alternative name(s):
Calpain inhibitor
Sperm BS-17 component

Gene names

Name:

CAST

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	708 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Domain	Each of the four flexible inhibitory domains can inhibit one calcium-bound calpain molecule by occupying both sides of the active site By similarity.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the protease inhibitor I27 (calpastatin) family. [View classification]
Sequence caution	The sequence AAH16066.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Molecular function	Protease inhibitor Thiol protease inhibitor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	cysteine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW endopeptidase inhibitor activity Traceable author statement PubMed 7638179. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NCK1	P16333	2	EBI-1268770,EBI-389883

Alternative products

This entry describes 8 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P20810-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P20810-2)

The sequence of this isoform differs from the canonical sequence as follows:
     212-224: Missing.

Isoform 3 (identifier: P20810-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.
     212-224: Missing.

Isoform 4 (identifier: P20810-4)

The sequence of this isoform differs from the canonical sequence as follows:
     9-30: Missing.
     44-62: Missing.

Isoform 5 (identifier: P20810-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: MNPTETKAIPVSQQMEGPHLPNKKKHKKQ → MSQPGQKPAA...SSSMNPTETK
     212-224: Missing.

Note: Contains a phosphoserine at position 11.

Isoform 6 (identifier: P20810-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSQPGQKPAA...SGATSKSSSM

Note: Contains a phosphoserine at position 11.

Isoform 7 (identifier: P20810-7)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSQPGQKPAA...SGATSKSSSM
     44-62: Missing.

Note: Contains a phosphoserine at position 11.

Isoform 8 (identifier: P20810-8)

The sequence of this isoform differs from the canonical sequence as follows:
     9-30: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 708	708	Calpastatin		PRO_0000147632
Regions
	Repeat	170 – 222	53	Inhibitory domain 1
	Repeat	304 – 356	53	Inhibitory domain 2
	Repeat	446 – 499	54	Inhibitory domain 3
	Repeat	583 – 636	54	Inhibitory domain 4
Amino acid modifications
	Modified residue	87	1	Phosphoserine By similarity
	Modified residue	133	1	Phosphoserine Ref.19
	Modified residue	222	1	Phosphoserine By similarity
	Modified residue	243	1	Phosphoserine Ref.18
	Modified residue	364	1	Phosphoserine Ref.18
	Modified residue	366	1	Phosphoserine Ref.18
	Modified residue	373	1	Phosphoserine Ref.18
	Modified residue	575	1	Phosphoserine Ref.19
	Modified residue	577	1	Phosphoserine Ref.19
Natural variations
	Alternative sequence	1 – 105	105	Missing in isoform 3.		VSP_000741
	Alternative sequence	1 – 29	29	MNPTE…KHKKQ → MSQPGQKPAASPRPRRAAAA RRTHEHVSEKTSESPSKPGE KKGSDEKKAASLGSSQSSRT YAGGTASATKVSASSGATSK SSSMNPTETK in isoform 5.		VSP_038037
	Alternative sequence	1	1	M → MSQPGQKPAASPRPRRAAAA RRTHEHVSEKTSESPSKPGE KKGSDEKKAASLGSSQSSRT YAGGTASATKVSASSGATSK SSSM in isoform 6 and isoform 7.		VSP_038038
	Alternative sequence	9 – 30	22	Missing in isoform 4 and isoform 8.		VSP_000742
	Alternative sequence	44 – 62	19	Missing in isoform 4 and isoform 7.		VSP_000743
	Alternative sequence	212 – 224	13	Missing in isoform 2, isoform 3 and isoform 5.		VSP_000744
	Natural variant	380	1	E → K. Corresponds to variant rs1643702 [ dbSNP | Ensembl ].		VAR_030741
	Natural variant	408	1	C → S. Ref.1 Ref.7 Ref.11 Ref.14 Corresponds to variant rs754615 [ dbSNP | Ensembl ].		VAR_022686
	Natural variant	537	1	A → V. Corresponds to variant rs4948 [ dbSNP | Ensembl ].		VAR_030742
	Natural variant	592	1	E → G. Ref.1		VAR_005298
Experimental info
	Sequence conflict	467	1	R → L in AAC50136. Ref.11
	Sequence conflict	486 – 488	3	VKD → GKE in AAC50136. Ref.11
	Sequence conflict	543	1	V → L in AAC50136. Ref.11
	Sequence conflict	562	1	Missing in AAB60371. Ref.14
	Sequence conflict	651	1	I → V in AAD09102. Ref.15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 20, 2007. Version 4. Checksum: 2DB0C14B860E89C7	FASTA	708	76,573
	10 20 30 40 50 60 MNPTETKAIP VSQQMEGPHL PNKKKHKKQA VKTEPEKKSQ STKLSVVHEK KSQEGKPKEH 70 80 90 100 110 120 TEPKSLPKQA SDTGSNDAHN KKAVSRSAEQ QPSEKSTEPK TKPQDMISAG GESVAGITAI 130 140 150 160 170 180 SGKPGDKKKE KKSLTPAVPV ESKPDKPSGK SGMDAALDDL IDTLGGPEET EEENTTYTGP 190 200 210 220 230 240 EVSDPMSSTY IEELGKREVT IPPKYRELLA KKEGITGPPA DSSKPIGPDD AIDALSSDFT 250 260 270 280 290 300 CGSPTAAGKK TEKEESTEVL KAQSAGTVRS AAPPQEKKRK VEKDTMSDQA LEALSASLGT 310 320 330 340 350 360 RQAEPELDLR SIKEVDEAKA KEEKLEKCGE DDETIPSEYR LKPATDKDGK PLLPEPEEKP 370 380 390 400 410 420 KPRSESELID ELSEDFDRSE CKEKPSKPTE KTEESKAAAP APVSEAVCRT SMCSIQSAPP 430 440 450 460 470 480 EPATLKGTVP DDAVEALADS LGKKEADPED GKPVMDKVKE KAKEEDREKL GEKEETIPPD 490 500 510 520 530 540 YRLEEVKDKD GKPLLPKESK EQLPPMSEDF LLDALSEDFS GPQNASSLKF EDAKLAAAIS 550 560 570 580 590 600 EVVSQTPAST TQAGAPPRDT SQSDKDLDDA LDKLSDSLGQ RQPDPDENKP MEDKVKEKAK 610 620 630 640 650 660 AEHRDKLGER DDTIPPEYRH LLDDNGQDKP VKPPTKKSED SKKPADDQDP IDALSGDLDS 670 680 690 700 CPSTTETSQN TAKDKCKKAA SSSKAPKNGG KAKDSAKTTE ETSKPKDD
	Isoform 2 [UniParc]. Checksum: 9DBFF022F785D117	FASTA	695	75,304
	10 20 30 40 50 60 MNPTETKAIP VSQQMEGPHL PNKKKHKKQA VKTEPEKKSQ STKLSVVHEK KSQEGKPKEH 70 80 90 100 110 120 TEPKSLPKQA SDTGSNDAHN KKAVSRSAEQ QPSEKSTEPK TKPQDMISAG GESVAGITAI 130 140 150 160 170 180 SGKPGDKKKE KKSLTPAVPV ESKPDKPSGK SGMDAALDDL IDTLGGPEET EEENTTYTGP 190 200 210 220 230 240 EVSDPMSSTY IEELGKREVT IPPKYRELLA KPIGPDDAID ALSSDFTCGS PTAAGKKTEK 250 260 270 280 290 300 EESTEVLKAQ SAGTVRSAAP PQEKKRKVEK DTMSDQALEA LSASLGTRQA EPELDLRSIK 310 320 330 340 350 360 EVDEAKAKEE KLEKCGEDDE TIPSEYRLKP ATDKDGKPLL PEPEEKPKPR SESELIDELS 370 380 390 400 410 420 EDFDRSECKE KPSKPTEKTE ESKAAAPAPV SEAVCRTSMC SIQSAPPEPA TLKGTVPDDA 430 440 450 460 470 480 VEALADSLGK KEADPEDGKP VMDKVKEKAK EEDREKLGEK EETIPPDYRL EEVKDKDGKP 490 500 510 520 530 540 LLPKESKEQL PPMSEDFLLD ALSEDFSGPQ NASSLKFEDA KLAAAISEVV SQTPASTTQA 550 560 570 580 590 600 GAPPRDTSQS DKDLDDALDK LSDSLGQRQP DPDENKPMED KVKEKAKAEH RDKLGERDDT 610 620 630 640 650 660 IPPEYRHLLD DNGQDKPVKP PTKKSEDSKK PADDQDPIDA LSGDLDSCPS TTETSQNTAK 670 680 690 DKCKKAASSS KAPKNGGKAK DSAKTTEETS KPKDD
	Isoform 3 [UniParc]. Checksum: DB776EDB8EC13005	FASTA	590	63,665
	10 20 30 40 50 60 MISAGGESVA GITAISGKPG DKKKEKKSLT PAVPVESKPD KPSGKSGMDA ALDDLIDTLG 70 80 90 100 110 120 GPEETEEENT TYTGPEVSDP MSSTYIEELG KREVTIPPKY RELLAKPIGP DDAIDALSSD 130 140 150 160 170 180 FTCGSPTAAG KKTEKEESTE VLKAQSAGTV RSAAPPQEKK RKVEKDTMSD QALEALSASL 190 200 210 220 230 240 GTRQAEPELD LRSIKEVDEA KAKEEKLEKC GEDDETIPSE YRLKPATDKD GKPLLPEPEE 250 260 270 280 290 300 KPKPRSESEL IDELSEDFDR SECKEKPSKP TEKTEESKAA APAPVSEAVC RTSMCSIQSA 310 320 330 340 350 360 PPEPATLKGT VPDDAVEALA DSLGKKEADP EDGKPVMDKV KEKAKEEDRE KLGEKEETIP 370 380 390 400 410 420 PDYRLEEVKD KDGKPLLPKE SKEQLPPMSE DFLLDALSED FSGPQNASSL KFEDAKLAAA 430 440 450 460 470 480 ISEVVSQTPA STTQAGAPPR DTSQSDKDLD DALDKLSDSL GQRQPDPDEN KPMEDKVKEK 490 500 510 520 530 540 AKAEHRDKLG ERDDTIPPEY RHLLDDNGQD KPVKPPTKKS EDSKKPADDQ DPIDALSGDL 550 560 570 580 590 DSCPSTTETS QNTAKDKCKK AASSSKAPKN GGKAKDSAKT TEETSKPKDD
	Isoform 4 [UniParc]. Checksum: 47831647A417E024	FASTA	667	71,894
	10 20 30 40 50 60 MNPTETKAVK TEPEKKSQST KPKSLPKQAS DTGSNDAHNK KAVSRSAEQQ PSEKSTEPKT 70 80 90 100 110 120 KPQDMISAGG ESVAGITAIS GKPGDKKKEK KSLTPAVPVE SKPDKPSGKS GMDAALDDLI 130 140 150 160 170 180 DTLGGPEETE EENTTYTGPE VSDPMSSTYI EELGKREVTI PPKYRELLAK KEGITGPPAD 190 200 210 220 230 240 SSKPIGPDDA IDALSSDFTC GSPTAAGKKT EKEESTEVLK AQSAGTVRSA APPQEKKRKV 250 260 270 280 290 300 EKDTMSDQAL EALSASLGTR QAEPELDLRS IKEVDEAKAK EEKLEKCGED DETIPSEYRL 310 320 330 340 350 360 KPATDKDGKP LLPEPEEKPK PRSESELIDE LSEDFDRSEC KEKPSKPTEK TEESKAAAPA 370 380 390 400 410 420 PVSEAVCRTS MCSIQSAPPE PATLKGTVPD DAVEALADSL GKKEADPEDG KPVMDKVKEK 430 440 450 460 470 480 AKEEDREKLG EKEETIPPDY RLEEVKDKDG KPLLPKESKE QLPPMSEDFL LDALSEDFSG 490 500 510 520 530 540 PQNASSLKFE DAKLAAAISE VVSQTPASTT QAGAPPRDTS QSDKDLDDAL DKLSDSLGQR 550 560 570 580 590 600 QPDPDENKPM EDKVKEKAKA EHRDKLGERD DTIPPEYRHL LDDNGQDKPV KPPTKKSEDS 610 620 630 640 650 660 KKPADDQDPI DALSGDLDSC PSTTETSQNT AKDKCKKAAS SSKAPKNGGK AKDSAKTTEE TSKPKDD
	Isoform 5 [UniParc]. Checksum: 18BA3EAB0A9989D3	FASTA	756	81,169
	10 20 30 40 50 60 MSQPGQKPAA SPRPRRAAAA RRTHEHVSEK TSESPSKPGE KKGSDEKKAA SLGSSQSSRT 70 80 90 100 110 120 YAGGTASATK VSASSGATSK SSSMNPTETK AVKTEPEKKS QSTKLSVVHE KKSQEGKPKE 130 140 150 160 170 180 HTEPKSLPKQ ASDTGSNDAH NKKAVSRSAE QQPSEKSTEP KTKPQDMISA GGESVAGITA 190 200 210 220 230 240 ISGKPGDKKK EKKSLTPAVP VESKPDKPSG KSGMDAALDD LIDTLGGPEE TEEENTTYTG 250 260 270 280 290 300 PEVSDPMSST YIEELGKREV TIPPKYRELL AKPIGPDDAI DALSSDFTCG SPTAAGKKTE 310 320 330 340 350 360 KEESTEVLKA QSAGTVRSAA PPQEKKRKVE KDTMSDQALE ALSASLGTRQ AEPELDLRSI 370 380 390 400 410 420 KEVDEAKAKE EKLEKCGEDD ETIPSEYRLK PATDKDGKPL LPEPEEKPKP RSESELIDEL 430 440 450 460 470 480 SEDFDRSECK EKPSKPTEKT EESKAAAPAP VSEAVCRTSM CSIQSAPPEP ATLKGTVPDD 490 500 510 520 530 540 AVEALADSLG KKEADPEDGK PVMDKVKEKA KEEDREKLGE KEETIPPDYR LEEVKDKDGK 550 560 570 580 590 600 PLLPKESKEQ LPPMSEDFLL DALSEDFSGP QNASSLKFED AKLAAAISEV VSQTPASTTQ 610 620 630 640 650 660 AGAPPRDTSQ SDKDLDDALD KLSDSLGQRQ PDPDENKPME DKVKEKAKAE HRDKLGERDD 670 680 690 700 710 720 TIPPEYRHLL DDNGQDKPVK PPTKKSEDSK KPADDQDPID ALSGDLDSCP STTETSQNTA 730 740 750 KDKCKKAASS SKAPKNGGKA KDSAKTTEET SKPKDD
	Isoform 6 [UniParc]. Checksum: DC3DC0A4FC0F0825	FASTA	791	84,943
	10 20 30 40 50 60 MSQPGQKPAA SPRPRRAAAA RRTHEHVSEK TSESPSKPGE KKGSDEKKAA SLGSSQSSRT 70 80 90 100 110 120 YAGGTASATK VSASSGATSK SSSMNPTETK AIPVSQQMEG PHLPNKKKHK KQAVKTEPEK 130 140 150 160 170 180 KSQSTKLSVV HEKKSQEGKP KEHTEPKSLP KQASDTGSND AHNKKAVSRS AEQQPSEKST 190 200 210 220 230 240 EPKTKPQDMI SAGGESVAGI TAISGKPGDK KKEKKSLTPA VPVESKPDKP SGKSGMDAAL 250 260 270 280 290 300 DDLIDTLGGP EETEEENTTY TGPEVSDPMS STYIEELGKR EVTIPPKYRE LLAKKEGITG 310 320 330 340 350 360 PPADSSKPIG PDDAIDALSS DFTCGSPTAA GKKTEKEEST EVLKAQSAGT VRSAAPPQEK 370 380 390 400 410 420 KRKVEKDTMS DQALEALSAS LGTRQAEPEL DLRSIKEVDE AKAKEEKLEK CGEDDETIPS 430 440 450 460 470 480 EYRLKPATDK DGKPLLPEPE EKPKPRSESE LIDELSEDFD RSECKEKPSK PTEKTEESKA 490 500 510 520 530 540 AAPAPVSEAV CRTSMCSIQS APPEPATLKG TVPDDAVEAL ADSLGKKEAD PEDGKPVMDK 550 560 570 580 590 600 VKEKAKEEDR EKLGEKEETI PPDYRLEEVK DKDGKPLLPK ESKEQLPPMS EDFLLDALSE 610 620 630 640 650 660 DFSGPQNASS LKFEDAKLAA AISEVVSQTP ASTTQAGAPP RDTSQSDKDL DDALDKLSDS 670 680 690 700 710 720 LGQRQPDPDE NKPMEDKVKE KAKAEHRDKL GERDDTIPPE YRHLLDDNGQ DKPVKPPTKK 730 740 750 760 770 780 SEDSKKPADD QDPIDALSGD LDSCPSTTET SQNTAKDKCK KAASSSKAPK NGGKAKDSAK 790 TTEETSKPKD D
	Isoform 7 [UniParc]. Checksum: 1B147E0CE4CD15E9	FASTA	772	82,771
	10 20 30 40 50 60 MSQPGQKPAA SPRPRRAAAA RRTHEHVSEK TSESPSKPGE KKGSDEKKAA SLGSSQSSRT 70 80 90 100 110 120 YAGGTASATK VSASSGATSK SSSMNPTETK AIPVSQQMEG PHLPNKKKHK KQAVKTEPEK 130 140 150 160 170 180 KSQSTKPKSL PKQASDTGSN DAHNKKAVSR SAEQQPSEKS TEPKTKPQDM ISAGGESVAG 190 200 210 220 230 240 ITAISGKPGD KKKEKKSLTP AVPVESKPDK PSGKSGMDAA LDDLIDTLGG PEETEEENTT 250 260 270 280 290 300 YTGPEVSDPM SSTYIEELGK REVTIPPKYR ELLAKKEGIT GPPADSSKPI GPDDAIDALS 310 320 330 340 350 360 SDFTCGSPTA AGKKTEKEES TEVLKAQSAG TVRSAAPPQE KKRKVEKDTM SDQALEALSA 370 380 390 400 410 420 SLGTRQAEPE LDLRSIKEVD EAKAKEEKLE KCGEDDETIP SEYRLKPATD KDGKPLLPEP 430 440 450 460 470 480 EEKPKPRSES ELIDELSEDF DRSECKEKPS KPTEKTEESK AAAPAPVSEA VCRTSMCSIQ 490 500 510 520 530 540 SAPPEPATLK GTVPDDAVEA LADSLGKKEA DPEDGKPVMD KVKEKAKEED REKLGEKEET 550 560 570 580 590 600 IPPDYRLEEV KDKDGKPLLP KESKEQLPPM SEDFLLDALS EDFSGPQNAS SLKFEDAKLA 610 620 630 640 650 660 AAISEVVSQT PASTTQAGAP PRDTSQSDKD LDDALDKLSD SLGQRQPDPD ENKPMEDKVK 670 680 690 700 710 720 EKAKAEHRDK LGERDDTIPP EYRHLLDDNG QDKPVKPPTK KSEDSKKPAD DQDPIDALSG 730 740 750 760 770 DLDSCPSTTE TSQNTAKDKC KKAASSSKAP KNGGKAKDSA KTTEETSKPK DD
	Isoform 8 [UniParc]. Checksum: 3B383857507798D3	FASTA	686	74,067
	10 20 30 40 50 60 MNPTETKAVK TEPEKKSQST KLSVVHEKKS QEGKPKEHTE PKSLPKQASD TGSNDAHNKK 70 80 90 100 110 120 AVSRSAEQQP SEKSTEPKTK PQDMISAGGE SVAGITAISG KPGDKKKEKK SLTPAVPVES 130 140 150 160 170 180 KPDKPSGKSG MDAALDDLID TLGGPEETEE ENTTYTGPEV SDPMSSTYIE ELGKREVTIP 190 200 210 220 230 240 PKYRELLAKK EGITGPPADS SKPIGPDDAI DALSSDFTCG SPTAAGKKTE KEESTEVLKA 250 260 270 280 290 300 QSAGTVRSAA PPQEKKRKVE KDTMSDQALE ALSASLGTRQ AEPELDLRSI KEVDEAKAKE 310 320 330 340 350 360 EKLEKCGEDD ETIPSEYRLK PATDKDGKPL LPEPEEKPKP RSESELIDEL SEDFDRSECK 370 380 390 400 410 420 EKPSKPTEKT EESKAAAPAP VSEAVCRTSM CSIQSAPPEP ATLKGTVPDD AVEALADSLG 430 440 450 460 470 480 KKEADPEDGK PVMDKVKEKA KEEDREKLGE KEETIPPDYR LEEVKDKDGK PLLPKESKEQ 490 500 510 520 530 540 LPPMSEDFLL DALSEDFSGP QNASSLKFED AKLAAAISEV VSQTPASTTQ AGAPPRDTSQ 550 560 570 580 590 600 SDKDLDDALD KLSDSLGQRQ PDPDENKPME DKVKEKAKAE HRDKLGERDD TIPPEYRHLL 610 620 630 640 650 660 DDNGQDKPVK PPTKKSEDSK KPADDQDPID ALSGDLDSCP STTETSQNTA KDKCKKAASS 670 680 SKAPKNGGKA KDSAKTTEET SKPKDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of human calpastatin: sequence homology among human, pig, and rabbit calpastatins." Asada K., Ishino Y., Shimada M., Shimojo T., Endo M., Kimizuka F., Kato I., Maki M., Hatanaka M., Murachi T. J. Enzym. Inhib. 3:49-56(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS SER-408 AND GLY-592.
[2]	"Cloning and characterization of a novel isoform of calpastatin in human adult testis." Zhu H., Zhou Z.-M., Li J.-M., Zhu H., Cheng L.-J., Shan Y.-X., Yin L.-L., Sha J.H. Acta Pharmacol. Sin. 23:450-454(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Testis.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
[4]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 6 AND 7).
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-319 (ISOFORM 5). Tissue: Lung and Skin.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-479 (ISOFORM 8), VARIANT SER-408.
[8]	"Molecular diversity in amino-terminal domains of human calpastatin by exon skipping." Lee W.J., Ma H., Takano E., Yang H.Q., Hatanaka M., Maki M. J. Biol. Chem. 267:8437-8442(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-283 (ISOFORM 1), ALTERNATIVE SPLICING. Tissue: Lung.
[9]	"Inhibition of calpain by a synthetic oligopeptide corresponding to an exon of the human calpastatin gene." Maki M., Bagci H., Hamaguchi K., Ueda M., Murachi T., Hatanaka M. J. Biol. Chem. 264:18866-18869(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 101-317.
[10]	"Characterization of a functional domain of human calpastatin." Uemori T., Shimojo T., Asada K., Asano T., Kimizuka F., Kato I., Maki M., Hatanaka M., Murachi T., Hanzawa H., Arata Y. Biochem. Biophys. Res. Commun. 166:1485-1493(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 125-283 (ISOFORMS 2/3).
[11]	Wang L.F. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 153-708 (ISOFORMS 2/3), SEQUENCE REVISION, VARIANT SER-408.
[12]	"Calpastatin gene in human testis." Wang L.F., Wei S.G., Miao S.Y., Liu Q.Y., Koide S.S. Biochem. Mol. Biol. Int. 33:245-252(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 523-708 (ISOFORMS 1/2/3/4). Tissue: Testis.
[13]	"Detection and expression of a cDNA clone that encodes a polypeptide containing two inhibitory domains of human calpastatin and its recognition by rheumatoid arthritis sera." Despres N., Talbot G., Plouffe B., Boire G., Menard H.A. J. Clin. Invest. 95:1891-1896(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 425-708 (ISOFORMS 1/2/3/4). Tissue: Placenta.
[14]	El-Amine M., Talbot G., Despres N., Asselin C., Boire G., Menard H.A. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 244-708 (ISOFORMS 1/2/3/4), VARIANT SER-408. Tissue: Placenta.
[15]	"New calpastatin 3' UTR." Rochdi M.D., El-Amine M., Menard H.A. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 636-708 (ISOFORMS 1/2/3/4).
[16]	"Phosphorylation and subcellular distribution of calpastatin in human hematopoietic system cells." Adachi Y., Ishida-Takahashi A., Takahashi C., Takano E., Murachi T., Hatanaka M. J. Biol. Chem. 266:3968-3972(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION.
[17]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[18]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-243; SER-364; SER-366 AND SER-373, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[19]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133; SER-575 AND SER-577, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11 (ISOFORMS 5; 6 AND 7), MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[20]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16217 mRNA. Translation: BAA03747.1. D50827 mRNA. Translation: BAA09438.1. AF327443 mRNA. Translation: AAG48151.1. BT009783 mRNA. Translation: AAP88785.1. AC008906 Genomic DNA. No translation available. AC020900 Genomic DNA. No translation available. AC104125 Genomic DNA. No translation available. CH471084 Genomic DNA. Translation: EAW96068.1. CH471084 Genomic DNA. Translation: EAW96075.1. BC013579 mRNA. Translation: AAH13579.1. BC016066 mRNA. Translation: AAH16066.1. Sequence problems. AL832349 mRNA. No translation available. M86258 mRNA. Translation: AAB59398.1. M28230 , M28227, M28228, M28229 Genomic DNA. Translation: AAA52066.1. M33328 mRNA. Translation: AAA52296.1. U26724 mRNA. Translation: AAC50136.2. S73329 mRNA. Translation: AAB32311.1. U38525 mRNA. Translation: AAA80684.1. U31345 mRNA. Translation: AAB60371.1. U31346 mRNA. Translation: AAB60372.1. AF095891 mRNA. Translation: AAD09102.1.
IPI	IPI00220857. IPI00305750. IPI00413492. IPI00760715. IPI00761035. IPI00761160. IPI00979393.
PIR	A38091.
RefSeq	NP_001035905.1. NM_001042440.2. NP_001177371.1. NM_001190442.1. NP_775083.1. NM_173060.3.
UniGene	Hs.436186.
3D structure databases
DisProt	DP00196.
ProteinModelPortal	P20810.
ModBase	Search...
Protein-protein interaction databases
IntAct	P20810. 8 interactions.
STRING	9606.ENSP00000379158.
Protein family/group databases
MEROPS	I27.001.
PTM databases
PhosphoSite	P20810.
Polymorphism databases
DMDM	126302556.
Proteomic databases
PaxDb	P20810.
PRIDE	P20810.
Protocols and materials databases
DNASU	831.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000309190; ENSP00000312523; ENSG00000153113. ENST00000325674; ENSP00000320319; ENSG00000153113. ENST00000338252; ENSP00000343421; ENSG00000153113. ENST00000341926; ENSP00000339914; ENSG00000153113. ENST00000359176; ENSP00000352098; ENSG00000153113. ENST00000395813; ENSP00000379158; ENSG00000153113. ENST00000508830; ENSP00000425721; ENSG00000153113. ENST00000510098; ENSP00000427195; ENSG00000153113.
GeneID	831.
KEGG	hsa:831.
UCSC	uc003klt.3. human. uc003klz.1. human. uc003kma.2. human.
Organism-specific databases
CTD	831.
GeneCards	GC05P095865.
HGNC	HGNC:1515. CAST.
HPA	CAB009491.
MIM	114090. gene.
neXtProt	NX_P20810.
PharmGKB	PA26098.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG25116.
HOGENOM	HOG000082405.
HOVERGEN	HBG000183.
InParanoid	P20810.
KO	K04281.
OMA	GPDDAID.
Gene expression databases
ArrayExpress	P20810.
Bgee	P20810.
CleanEx	HS_CAST.
Genevestigator	P20810.
GermOnline	ENSG00000153113. Homo sapiens.
Family and domain databases
InterPro	IPR026998. Calpastatin. IPR001259. Prot_inh_calpain. [Graphical view]
PANTHER	PTHR10077. PTHR10077. 1 hit.
Pfam	PF00748. Calpain_inhib. 4 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CAST. human.
GenomeRNAi	831.
NextBio	3422.
SOURCE	Search...

Entry information

Entry name

ICAL_HUMAN

Accession

Primary (citable) accession number: P20810
Secondary accession number(s): G5E946 , O95360, Q05DE8, Q7Z4K0, Q96D08, Q9H1Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 20, 2007
Last modified:	May 1, 2013
This is version 127 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.