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P20805

- GLNA2_FRAAL

UniProt

P20805 - GLNA2_FRAAL

Protein

Glutamine synthetase 2

Gene

glnII

Organism
Frankia alni
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Assimilates ammonia during nitrogen limitation. May also have a role in plant symbioses.

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW
    3. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation, Stress response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase 2 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase II
    Glutamine synthetase II
    Short name:
    GSII
    Gene namesi
    Name:glnII
    OrganismiFrankia alni
    Taxonomic identifieri1859 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 352352Glutamine synthetase 2PRO_0000153222Add
    BLAST

    Expressioni

    Inductioni

    By nitrogen starvation.

    Interactioni

    Subunit structurei

    Homooctamer.

    Structurei

    3D structure databases

    ProteinModelPortaliP20805.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20805-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYQAEYIWI DGTEPEPLMR SKTRIIKDGK EPEIWGFDGS STNQAPGSNS    50
    DCVLRPVFET PDPIRGGDNR LVLCEVQLTD FTPPTNTRAA ALGVAERYAD 100
    MSPMFGIEQE YTFFKDGRPY GWPEVGYPAP QGPYYCGVGG SKMPGRQIVE 150
    RHTQACLDAG LAIEGTNAEV MMGQWEFQIG VLPAPAIGDQ IWLGRWLLHR 200
    IAEDYGVEVS FAAKPIPGDW NGAGAHTNFS TKQTMEGWDA IVTCCEALGT 250
    RVTEHVTHYG KGIEDRLTGK HETAPWNKYS WGASDRGASV RIPWAVEKAK 300
    KGWLEDRRPN ANMDPYLVTA LMIDTCCSAL AGDKPTLFVP SQTTPAPAEA 350
    SV 352
    Length:352
    Mass (Da):38,666
    Last modified:February 1, 1991 - v1
    Checksum:i2BFCC8B8A87C3340
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58415 Genomic DNA. Translation: AAA62803.1.
    L10632 Genomic DNA. No translation available.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58415 Genomic DNA. Translation: AAA62803.1 .
    L10632 Genomic DNA. No translation available.

    3D structure databases

    ProteinModelPortali P20805.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, sequencing, and expression of the glutamine synthetase II (glnII) gene from the actinomycete root nodule symbiont Frankia sp. strain CpI1."
      Rochefort D.A., Benson D.R.
      J. Bacteriol. 172:5335-5342(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: CpI1.
    2. "Close linkage of genes encoding glutamine synthetases I and II in Frankia alni CpI1."
      Hosted T.J., Rochefort D.A., Benson D.R.
      J. Bacteriol. 175:3679-3684(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
      Strain: CpI1.

    Entry informationi

    Entry nameiGLNA2_FRAAL
    AccessioniPrimary (citable) accession number: P20805
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3