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Protein

Glutamine synthetase

Gene

glnII

Organism
Frankia alni
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Miscellaneous

Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.1 Publication

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi108MagnesiumBy similarity1
Metal bindingi110MagnesiumBy similarity1
Binding sitei164ATPBy similarity1
Metal bindingi169MagnesiumBy similarity1
Metal bindingi176MagnesiumBy similarity1
Binding sitei272L-glutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation, Stress response
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase1 Publication (EC:6.3.1.2By similarity)
Short name:
GS1 Publication
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase II1 Publication
Short name:
GSII1 Publication
Gene namesi
Name:glnII1 Publication
OrganismiFrankia alni
Taxonomic identifieri1859 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532221 – 352Glutamine synthetaseAdd BLAST352

Expressioni

Inductioni

By nitrogen starvation.1 Publication

Interactioni

Subunit structurei

Homooctamer and homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP20805.
SMRiP20805.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 2 hits.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P20805-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYQAEYIWI DGTEPEPLMR SKTRIIKDGK EPEIWGFDGS STNQAPGSNS
60 70 80 90 100
DCVLRPVFET PDPIRGGDNR LVLCEVQLTD FTPPTNTRAA ALGVAERYAD
110 120 130 140 150
MSPMFGIEQE YTFFKDGRPY GWPEVGYPAP QGPYYCGVGG SKMPGRQIVE
160 170 180 190 200
RHTQACLDAG LAIEGTNAEV MMGQWEFQIG VLPAPAIGDQ IWLGRWLLHR
210 220 230 240 250
IAEDYGVEVS FAAKPIPGDW NGAGAHTNFS TKQTMEGWDA IVTCCEALGT
260 270 280 290 300
RVTEHVTHYG KGIEDRLTGK HETAPWNKYS WGASDRGASV RIPWAVEKAK
310 320 330 340 350
KGWLEDRRPN ANMDPYLVTA LMIDTCCSAL AGDKPTLFVP SQTTPAPAEA

SV
Length:352
Mass (Da):38,666
Last modified:February 1, 1991 - v1
Checksum:i2BFCC8B8A87C3340
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58415 Genomic DNA. Translation: AAA62803.1.
L10632 Genomic DNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58415 Genomic DNA. Translation: AAA62803.1.
L10632 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortaliP20805.
SMRiP20805.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 2 hits.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNA2_FRAAL
AccessioniPrimary (citable) accession number: P20805
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 12, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.