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P20805 (GLNA2_FRAAL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase 2

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase II
Glutamine synthetase II
Short name=GSII
Gene names
Name:glnII
OrganismFrankia alni
Taxonomic identifier1859 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Assimilates ammonia during nitrogen limitation. May also have a role in plant symbioses.

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Cytoplasm.

Induction

By nitrogen starvation.

Miscellaneous

Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Biological processNitrogen fixation
Stress response
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 352352Glutamine synthetase 2
PRO_0000153222

Sequences

Sequence LengthMass (Da)Tools
P20805 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 2BFCC8B8A87C3340

FASTA35238,666
        10         20         30         40         50         60 
MSYQAEYIWI DGTEPEPLMR SKTRIIKDGK EPEIWGFDGS STNQAPGSNS DCVLRPVFET 

        70         80         90        100        110        120 
PDPIRGGDNR LVLCEVQLTD FTPPTNTRAA ALGVAERYAD MSPMFGIEQE YTFFKDGRPY 

       130        140        150        160        170        180 
GWPEVGYPAP QGPYYCGVGG SKMPGRQIVE RHTQACLDAG LAIEGTNAEV MMGQWEFQIG 

       190        200        210        220        230        240 
VLPAPAIGDQ IWLGRWLLHR IAEDYGVEVS FAAKPIPGDW NGAGAHTNFS TKQTMEGWDA 

       250        260        270        280        290        300 
IVTCCEALGT RVTEHVTHYG KGIEDRLTGK HETAPWNKYS WGASDRGASV RIPWAVEKAK 

       310        320        330        340        350 
KGWLEDRRPN ANMDPYLVTA LMIDTCCSAL AGDKPTLFVP SQTTPAPAEA SV 

« Hide

References

[1]"Molecular cloning, sequencing, and expression of the glutamine synthetase II (glnII) gene from the actinomycete root nodule symbiont Frankia sp. strain CpI1."
Rochefort D.A., Benson D.R.
J. Bacteriol. 172:5335-5342(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CpI1.
[2]"Close linkage of genes encoding glutamine synthetases I and II in Frankia alni CpI1."
Hosted T.J., Rochefort D.A., Benson D.R.
J. Bacteriol. 175:3679-3684(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
Strain: CpI1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M58415 Genomic DNA. Translation: AAA62803.1.
L10632 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortalP20805.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA2_FRAAL
AccessionPrimary (citable) accession number: P20805
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: October 16, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families