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P20800 (EDN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endothelin-2

Short name=ET-2
Alternative name(s):
Preproendothelin-2
Short name=PPET2
Gene names
Name:EDN2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length178 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endothelins are endothelium-derived vasoconstrictor peptides.

Subcellular location

Secreted.

Tissue specificity

Expressed in lung, but not in placental stem villi vessels or cultured placental villi smooth muscle cells. Ref.10

Sequence similarities

Belongs to the endothelin/sarafotoxin family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionVasoactive
Vasoconstrictor
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processartery smooth muscle contraction

Inferred from direct assay PubMed 8982507. Source: BHF-UCL

calcium-mediated signaling

Inferred from direct assay PubMed 1917960. Source: BHF-UCL

cell surface receptor signaling pathway

Inferred by curator PubMed 1713452. Source: BHF-UCL

cytokine-mediated signaling pathway

Inferred from direct assay PubMed 12207323. Source: BHF-UCL

hormonal regulation of the force of heart contraction

Traceable author statement PubMed 1652300. Source: BHF-UCL

inositol phosphate-mediated signaling

Inferred from direct assay PubMed 1917960. Source: BHF-UCL

macrophage activation

Inferred from direct assay PubMed 12207323. Source: BHF-UCL

macrophage chemotaxis

Inferred from direct assay PubMed 12207323. Source: BHF-UCL

neutrophil chemotaxis

Inferred from direct assay PubMed 9696419. Source: BHF-UCL

positive regulation of cell proliferation

Inferred from direct assay PubMed 1917960. Source: BHF-UCL

positive regulation of cytosolic calcium ion concentration

Inferred from direct assay PubMed 1917960. Source: BHF-UCL

positive regulation of heart rate

Inferred from direct assay Ref.8. Source: BHF-UCL

positive regulation of leukocyte chemotaxis

Inferred from direct assay PubMed 9696419. Source: BHF-UCL

positive regulation of prostaglandin-endoperoxide synthase activity

Inferred from mutant phenotype PubMed 9492062. Source: BHF-UCL

positive regulation of smooth muscle contraction

Inferred from electronic annotation. Source: Ensembl

positive regulation of the force of heart contraction by chemical signal

Traceable author statement PubMed 1652300. Source: BHF-UCL

prostaglandin biosynthetic process

Inferred from direct assay PubMed 9492062. Source: BHF-UCL

regulation of systemic arterial blood pressure by endothelin

Inferred from direct assay Ref.8. Source: BHF-UCL

regulation of vasoconstriction

Inferred from electronic annotation. Source: InterPro

vasoconstriction

Inferred from direct assay Ref.8PubMed 8982507. Source: BHF-UCL

vein smooth muscle contraction

Inferred from direct assay PubMed 8982507PubMed 9422810. Source: BHF-UCL

   Cellular_componentextracellular region

Traceable author statement. Source: Reactome

extracellular space

Inferred from direct assay PubMed 15691296. Source: BHF-UCL

   Molecular_functionhormone activity

Inferred from direct assay Ref.8. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 4622
PRO_0000008093
Peptide49 – 6921Endothelin-2
PRO_0000008094
Propeptide70 – 178109
PRO_0000008095

Regions

Region96 – 11116Endothelin-like

Sites

Site69 – 702Cleavage; by KEL

Amino acid modifications

Disulfide bond49 ↔ 63 By similarity
Disulfide bond51 ↔ 59 By similarity

Natural variations

Natural variant1311F → L.
Corresponds to variant rs5798 [ dbSNP | Ensembl ].
VAR_033914
Natural variant1681P → L. Ref.4
Corresponds to variant rs11572371 [ dbSNP | Ensembl ].
VAR_018817

Sequences

Sequence LengthMass (Da)Tools
P20800 [UniParc].

Last modified November 1, 1991. Version 2.
Checksum: 7711B2B6F075CFB4

FASTA17819,960
        10         20         30         40         50         60 
MVSVPTTWCS VALALLVALH EGKGQAAATL EQPASSSHAQ GTHLRLRRCS CSSWLDKECV 

        70         80         90        100        110        120 
YFCHLDIIWV NTPEQTAPYG LGNPPRRRRR SLPRRCQCSS ARDPACATFC LRRPWTEAGA 

       130        140        150        160        170 
VPSRKSPADV FQTGKTGATT GELLQRLRDI STVKSLFAKR QQEAMREPRS THSRWRKR 

« Hide

References

« Hide 'large scale' references
[1]"Specific expression of human endothelin-2 (ET-2) gene in a renal adenocarcinoma cell line. Molecular cloning of cDNA encoding the precursor of ET-2 and its characterization."
Ohkubo S., Ogi K., Hosoya M., Matsumoto H., Suzuki N., Kimura C., Onda H., Fujino M.
FEBS Lett. 274:136-140(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"cDNA cloning and chromosomal assignment of the endothelin 2 gene: vasoactive intestinal contractor peptide is rat endothelin 2."
Bloch K.D., Hong C.C., Eddy R.L. Jr., Shows T.B., Quertermous T.
Genomics 10:236-242(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Expression of endothelin-2 (ET-2) gene in a human renal adenocarcinoma cell line: purification and cDNA cloning of ET-2."
Onda H., Ohkubo S., Kosaka T., Yasuhara T., Ogi K., Hosoya M., Matsumoto H., Suzuki N., Kitada C., Ishibashi Y., Kimura C., Kubo K., Fujino M.
J. Cardiovasc. Pharmacol. 17:S39-S43(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]NIEHS SNPs program
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LEU-168.
[5]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon, Kidney and Stomach.
[8]"The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes."
Inoue A., Yanagisawa M., Kimura S., Kasuya Y., Miyauchi T., Goto K., Masaki T.
Proc. Natl. Acad. Sci. U.S.A. 86:2863-2867(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-73.
[9]"Proteolytic processing of big endothelin-3 by the kell blood group protein."
Lee S., Lin M., Mele A., Cao Y., Farmar J., Russo D., Redman C.
Blood 94:1440-1450(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, CLEAVAGE BY KELL, IDENTIFICATION BY MASS SPECTROMETRY.
[10]"Endothelin-1 and ETA receptor expression in vascular smooth muscle cells from human placenta: a new ETA receptor messenger ribonucleic acid is generated by alternative splicing of exon 3."
Bourgeois C., Robert B., Rebourcet R., Mondon F., Mignot T.-M., Duc-Goiran P., Ferre F.
J. Clin. Endocrinol. Metab. 82:3116-3123(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55177 mRNA. Translation: CAA38962.1.
M65199 mRNA. Translation: AAA52404.1.
AY518541 Genomic DNA. Translation: AAR89914.1.
AL445933 Genomic DNA. Translation: CAI14535.1.
CH471059 Genomic DNA. Translation: EAX07175.1.
BC034393 mRNA. Translation: AAH34393.1.
M25550 Genomic DNA. Translation: AAA52340.1.
CCDSCCDS462.1.
PIRA39070.
RefSeqNP_001947.1. NM_001956.3.
UniGeneHs.1407.

3D structure databases

ProteinModelPortalP20800.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000361668.

Polymorphism databases

DMDM119615.

Proteomic databases

PaxDbP20800.
PRIDEP20800.

Protocols and materials databases

DNASU1907.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000372587; ENSP00000361668; ENSG00000127129.
GeneID1907.
KEGGhsa:1907.
UCSCuc001cgx.3. human.

Organism-specific databases

CTD1907.
GeneCardsGC01M041944.
HGNCHGNC:3177. EDN2.
HPAHPA028459.
MIM131241. gene.
neXtProtNX_P20800.
PharmGKBPA27615.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG43933.
HOGENOMHOG000231110.
HOVERGENHBG051441.
InParanoidP20800.
KOK16367.
OMAEQTAPYG.
OrthoDBEOG7F7WBX.
PhylomeDBP20800.
TreeFamTF333184.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeP20800.
CleanExHS_EDN2.
GenevestigatorP20800.

Family and domain databases

InterProIPR020475. Bibrotoxin/Sarafotoxin-D.
IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
IPR015658. ET-2.
[Graphical view]
PANTHERPTHR13874:SF8. PTHR13874:SF8. 1 hit.
PfamPF00322. Endothelin. 1 hit.
[Graphical view]
PRINTSPR00365. ENDOTHELIN.
SMARTSM00272. END. 2 hits.
[Graphical view]
PROSITEPS00270. ENDOTHELIN. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiEndothelin_2.
GenomeRNAi1907.
NextBio7761.
PMAP-CutDBP20800.
PROP20800.
SOURCESearch...

Entry information

Entry nameEDN2_HUMAN
AccessionPrimary (citable) accession number: P20800
Secondary accession number(s): Q5T1R3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 1, 1991
Last modified: July 9, 2014
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM