P20800 (EDN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endothelin-2
Short name=ET-2

Alternative name(s):
Preproendothelin-2
Short name=PPET2

Gene names

Name:

EDN2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular location	Secreted.
Tissue specificity	Expressed in lung, but not in placental stem villi vessels or cultured placental villi smooth muscle cells. Ref.10
Sequence similarities	Belongs to the endothelin/sarafotoxin family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Vasoactive Vasoconstrictor
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	artery smooth muscle contraction Inferred from direct assay PubMed 8982507. Source: BHF-UCL calcium-mediated signaling Inferred from direct assay PubMed 1917960. Source: BHF-UCL cytokine-mediated signaling pathway Inferred from direct assay PubMed 12207323. Source: BHF-UCL elevation of cytosolic calcium ion concentration Inferred from direct assay PubMed 1917960. Source: BHF-UCL hormonal regulation of the force of heart contraction Traceable author statement PubMed 1652300. Source: BHF-UCL inositol phosphate-mediated signaling Inferred from direct assay PubMed 1917960. Source: BHF-UCL macrophage activation Inferred from direct assay PubMed 12207323. Source: BHF-UCL macrophage chemotaxis Inferred from direct assay PubMed 12207323. Source: BHF-UCL neutrophil chemotaxis Inferred from direct assay PubMed 9696419. Source: BHF-UCL positive regulation of cell proliferation Inferred from direct assay PubMed 1917960. Source: BHF-UCL positive regulation of heart rate Inferred from direct assay Ref.8. Source: BHF-UCL positive regulation of leukocyte chemotaxis Inferred from direct assay PubMed 9696419. Source: BHF-UCL positive regulation of prostaglandin-endoperoxide synthase activity Inferred from mutant phenotype PubMed 9492062. Source: BHF-UCL positive regulation of smooth muscle contraction Inferred from electronic annotation. Source: Compara positive regulation of the force of heart contraction by chemical signal Traceable author statement PubMed 1652300. Source: BHF-UCL prostaglandin biosynthetic process Inferred from direct assay PubMed 9492062. Source: BHF-UCL regulation of systemic arterial blood pressure by endothelin Inferred from direct assay Ref.8. Source: BHF-UCL regulation of vasoconstriction Inferred from electronic annotation. Source: InterPro vein smooth muscle contraction Inferred from direct assay PubMed 8982507PubMed 9422810. Source: BHF-UCL
Cellular_component	extracellular space Inferred from direct assay PubMed 15691296. Source: BHF-UCL
Molecular_function	hormone activity Inferred from direct assay Ref.8. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 46	22			PRO_0000008093
	Peptide	49 – 69	21	Endothelin-2		PRO_0000008094
	Propeptide	70 – 178	109			PRO_0000008095
Regions
	Region	96 – 111	16	Endothelin-like
Sites
	Site	69 – 70	2	Cleavage; by KEL
Amino acid modifications
	Disulfide bond	49 ↔ 63		By similarity
	Disulfide bond	51 ↔ 59		By similarity
Natural variations
	Natural variant	131	1	F → L. Corresponds to variant rs5798 [ dbSNP | Ensembl ].		VAR_033914
	Natural variant	168	1	P → L. Ref.4 Corresponds to variant rs11572371 [ dbSNP | Ensembl ].		VAR_018817

Sequences

	Sequence		Length	Mass (Da)
	P20800 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: 7711B2B6F075CFB4	FASTA	178	19,960
	10 20 30 40 50 60 MVSVPTTWCS VALALLVALH EGKGQAAATL EQPASSSHAQ GTHLRLRRCS CSSWLDKECV 70 80 90 100 110 120 YFCHLDIIWV NTPEQTAPYG LGNPPRRRRR SLPRRCQCSS ARDPACATFC LRRPWTEAGA 130 140 150 160 170 VPSRKSPADV FQTGKTGATT GELLQRLRDI STVKSLFAKR QQEAMREPRS THSRWRKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Specific expression of human endothelin-2 (ET-2) gene in a renal adenocarcinoma cell line. Molecular cloning of cDNA encoding the precursor of ET-2 and its characterization." Ohkubo S., Ogi K., Hosoya M., Matsumoto H., Suzuki N., Kimura C., Onda H., Fujino M. FEBS Lett. 274:136-140(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"cDNA cloning and chromosomal assignment of the endothelin 2 gene: vasoactive intestinal contractor peptide is rat endothelin 2." Bloch K.D., Hong C.C., Eddy R.L. Jr., Shows T.B., Quertermous T. Genomics 10:236-242(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Expression of endothelin-2 (ET-2) gene in a human renal adenocarcinoma cell line: purification and cDNA cloning of ET-2." Onda H., Ohkubo S., Kosaka T., Yasuhara T., Ogi K., Hosoya M., Matsumoto H., Suzuki N., Kitada C., Ishibashi Y., Kimura C., Kubo K., Fujino M. J. Cardiovasc. Pharmacol. 17:S39-S43(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	NIEHS SNPs program Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LEU-168.
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon, Kidney and Stomach.
[8]	"The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes." Inoue A., Yanagisawa M., Kimura S., Kasuya Y., Miyauchi T., Goto K., Masaki T. Proc. Natl. Acad. Sci. U.S.A. 86:2863-2867(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-73.
[9]	"Proteolytic processing of big endothelin-3 by the kell blood group protein." Lee S., Lin M., Mele A., Cao Y., Farmar J., Russo D., Redman C. Blood 94:1440-1450(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CLEAVAGE BY KELL, MASS SPECTROMETRY.
[10]	"Endothelin-1 and ETA receptor expression in vascular smooth muscle cells from human placenta: a new ETA receptor messenger ribonucleic acid is generated by alternative splicing of exon 3." Bourgeois C., Robert B., Rebourcet R., Mondon F., Mignot T.-M., Duc-Goiran P., Ferre F. J. Clin. Endocrinol. Metab. 82:3116-3123(1997) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55177 mRNA. Translation: CAA38962.1. M65199 mRNA. Translation: AAA52404.1. AY518541 Genomic DNA. Translation: AAR89914.1. AL445933 Genomic DNA. Translation: CAI14535.1. CH471059 Genomic DNA. Translation: EAX07175.1. BC034393 mRNA. Translation: AAH34393.1. M25550 Genomic DNA. Translation: AAA52340.1.
IPI	IPI00025816.
PIR	A39070.
RefSeq	NP_001947.1. NM_001956.3.
UniGene	Hs.1407.
3D structure databases
ProteinModelPortal	P20800.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000361668.
Polymorphism databases
DMDM	119615.
Proteomic databases
PaxDb	P20800.
PRIDE	P20800.
Protocols and materials databases
DNASU	1907.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000372587; ENSP00000361668; ENSG00000127129.
GeneID	1907.
KEGG	hsa:1907.
UCSC	uc001cgx.3. human.
Organism-specific databases
CTD	1907.
GeneCards	GC01M041944.
HGNC	HGNC:3177. EDN2.
HPA	HPA028459.
MIM	131241. gene.
neXtProt	NX_P20800.
PharmGKB	PA27615.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG43933.
HOGENOM	HOG000231110.
HOVERGEN	HBG051441.
InParanoid	P20800.
KO	K16367.
OMA	HLRLRRC.
OrthoDB	EOG4WH8N0.
PhylomeDB	P20800.
Enzyme and pathway databases
Pathway_Interaction_DB	endothelinpathway. Endothelins.
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P20800.
CleanEx	HS_EDN2.
Genevestigator	P20800.
GermOnline	ENSG00000127129. Homo sapiens.
Family and domain databases
InterPro	IPR020475. Bibrotoxin/Sarafotoxin-D. IPR019764. Endothelin_toxin_CS. IPR001928. Endothln-like_toxin. IPR015658. ET-2. [Graphical view]
PANTHER	PTHR13874:SF4. PTHR13874:SF4. 1 hit.
Pfam	PF00322. Endothelin. 1 hit. [Graphical view]
PRINTS	PR00365. ENDOTHELIN.
SMART	SM00272. END. 2 hits. [Graphical view]
PROSITE	PS00270. ENDOTHELIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	1907.
NextBio	7761.
PMAP-CutDB	P20800.
SOURCE	Search...

Entry information

Entry name

EDN2_HUMAN

Accession

Primary (citable) accession number: P20800
Secondary accession number(s): Q5T1R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 128 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.