Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P20800

- EDN2_HUMAN

UniProt

P20800 - EDN2_HUMAN

Protein

Endothelin-2

Gene

EDN2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Endothelins are endothelium-derived vasoconstrictor peptides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei69 – 702Cleavage; by KEL

    GO - Molecular functioni

    1. hormone activity Source: BHF-UCL

    GO - Biological processi

    1. artery smooth muscle contraction Source: BHF-UCL
    2. calcium-mediated signaling Source: BHF-UCL
    3. cell surface receptor signaling pathway Source: BHF-UCL
    4. cytokine-mediated signaling pathway Source: BHF-UCL
    5. hormonal regulation of the force of heart contraction Source: BHF-UCL
    6. inositol phosphate-mediated signaling Source: BHF-UCL
    7. macrophage activation Source: BHF-UCL
    8. macrophage chemotaxis Source: BHF-UCL
    9. neutrophil chemotaxis Source: BHF-UCL
    10. positive regulation of cell proliferation Source: BHF-UCL
    11. positive regulation of cytosolic calcium ion concentration Source: BHF-UCL
    12. positive regulation of heart rate Source: BHF-UCL
    13. positive regulation of leukocyte chemotaxis Source: BHF-UCL
    14. positive regulation of prostaglandin-endoperoxide synthase activity Source: BHF-UCL
    15. positive regulation of smooth muscle contraction Source: Ensembl
    16. positive regulation of the force of heart contraction by chemical signal Source: BHF-UCL
    17. prostaglandin biosynthetic process Source: BHF-UCL
    18. regulation of systemic arterial blood pressure by endothelin Source: BHF-UCL
    19. regulation of vasoconstriction Source: InterPro
    20. vasoconstriction Source: BHF-UCL
    21. vein smooth muscle contraction Source: BHF-UCL

    Keywords - Molecular functioni

    Vasoactive, Vasoconstrictor

    Enzyme and pathway databases

    ReactomeiREACT_14819. Peptide ligand-binding receptors.
    REACT_18283. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endothelin-2
    Short name:
    ET-2
    Alternative name(s):
    Preproendothelin-2
    Short name:
    PPET2
    Gene namesi
    Name:EDN2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:3177. EDN2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome
    2. extracellular space Source: BHF-UCL

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27615.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 4622PRO_0000008093Add
    BLAST
    Peptidei49 – 6921Endothelin-2PRO_0000008094Add
    BLAST
    Propeptidei70 – 178109PRO_0000008095Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi49 ↔ 63By similarity
    Disulfide bondi51 ↔ 59By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Proteomic databases

    PaxDbiP20800.
    PRIDEiP20800.

    Miscellaneous databases

    PMAP-CutDBP20800.

    Expressioni

    Tissue specificityi

    Expressed in lung, but not in placental stem villi vessels or cultured placental villi smooth muscle cells.1 Publication

    Gene expression databases

    BgeeiP20800.
    CleanExiHS_EDN2.
    GenevestigatoriP20800.

    Organism-specific databases

    HPAiHPA028459.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000361668.

    Structurei

    3D structure databases

    ProteinModelPortaliP20800.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni96 – 11116Endothelin-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the endothelin/sarafotoxin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG43933.
    HOGENOMiHOG000231110.
    HOVERGENiHBG051441.
    InParanoidiP20800.
    KOiK16367.
    OMAiEQTAPYG.
    OrthoDBiEOG7F7WBX.
    PhylomeDBiP20800.
    TreeFamiTF333184.

    Family and domain databases

    InterProiIPR020475. Bibrotoxin/Sarafotoxin-D.
    IPR019764. Endothelin_toxin_CS.
    IPR001928. Endothln-like_toxin.
    IPR015658. ET-2.
    [Graphical view]
    PANTHERiPTHR13874:SF8. PTHR13874:SF8. 1 hit.
    PfamiPF00322. Endothelin. 1 hit.
    [Graphical view]
    PRINTSiPR00365. ENDOTHELIN.
    SMARTiSM00272. END. 2 hits.
    [Graphical view]
    PROSITEiPS00270. ENDOTHELIN. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P20800-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSVPTTWCS VALALLVALH EGKGQAAATL EQPASSSHAQ GTHLRLRRCS    50
    CSSWLDKECV YFCHLDIIWV NTPEQTAPYG LGNPPRRRRR SLPRRCQCSS 100
    ARDPACATFC LRRPWTEAGA VPSRKSPADV FQTGKTGATT GELLQRLRDI 150
    STVKSLFAKR QQEAMREPRS THSRWRKR 178
    Length:178
    Mass (Da):19,960
    Last modified:November 1, 1991 - v2
    Checksum:i7711B2B6F075CFB4
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti131 – 1311F → L.
    Corresponds to variant rs5798 [ dbSNP | Ensembl ].
    VAR_033914
    Natural varianti168 – 1681P → L.1 Publication
    Corresponds to variant rs11572371 [ dbSNP | Ensembl ].
    VAR_018817

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55177 mRNA. Translation: CAA38962.1.
    M65199 mRNA. Translation: AAA52404.1.
    AY518541 Genomic DNA. Translation: AAR89914.1.
    AL445933 Genomic DNA. Translation: CAI14535.1.
    CH471059 Genomic DNA. Translation: EAX07175.1.
    BC034393 mRNA. Translation: AAH34393.1.
    M25550 Genomic DNA. Translation: AAA52340.1.
    CCDSiCCDS462.1.
    PIRiA39070.
    RefSeqiNP_001947.1. NM_001956.3.
    UniGeneiHs.1407.

    Genome annotation databases

    EnsembliENST00000372587; ENSP00000361668; ENSG00000127129.
    GeneIDi1907.
    KEGGihsa:1907.
    UCSCiuc001cgx.3. human.

    Polymorphism databases

    DMDMi119615.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55177 mRNA. Translation: CAA38962.1 .
    M65199 mRNA. Translation: AAA52404.1 .
    AY518541 Genomic DNA. Translation: AAR89914.1 .
    AL445933 Genomic DNA. Translation: CAI14535.1 .
    CH471059 Genomic DNA. Translation: EAX07175.1 .
    BC034393 mRNA. Translation: AAH34393.1 .
    M25550 Genomic DNA. Translation: AAA52340.1 .
    CCDSi CCDS462.1.
    PIRi A39070.
    RefSeqi NP_001947.1. NM_001956.3.
    UniGenei Hs.1407.

    3D structure databases

    ProteinModelPortali P20800.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000361668.

    Polymorphism databases

    DMDMi 119615.

    Proteomic databases

    PaxDbi P20800.
    PRIDEi P20800.

    Protocols and materials databases

    DNASUi 1907.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000372587 ; ENSP00000361668 ; ENSG00000127129 .
    GeneIDi 1907.
    KEGGi hsa:1907.
    UCSCi uc001cgx.3. human.

    Organism-specific databases

    CTDi 1907.
    GeneCardsi GC01M041944.
    HGNCi HGNC:3177. EDN2.
    HPAi HPA028459.
    MIMi 131241. gene.
    neXtProti NX_P20800.
    PharmGKBi PA27615.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG43933.
    HOGENOMi HOG000231110.
    HOVERGENi HBG051441.
    InParanoidi P20800.
    KOi K16367.
    OMAi EQTAPYG.
    OrthoDBi EOG7F7WBX.
    PhylomeDBi P20800.
    TreeFami TF333184.

    Enzyme and pathway databases

    Reactomei REACT_14819. Peptide ligand-binding receptors.
    REACT_18283. G alpha (q) signalling events.

    Miscellaneous databases

    GeneWikii Endothelin_2.
    GenomeRNAii 1907.
    NextBioi 7761.
    PMAP-CutDB P20800.
    PROi P20800.
    SOURCEi Search...

    Gene expression databases

    Bgeei P20800.
    CleanExi HS_EDN2.
    Genevestigatori P20800.

    Family and domain databases

    InterProi IPR020475. Bibrotoxin/Sarafotoxin-D.
    IPR019764. Endothelin_toxin_CS.
    IPR001928. Endothln-like_toxin.
    IPR015658. ET-2.
    [Graphical view ]
    PANTHERi PTHR13874:SF8. PTHR13874:SF8. 1 hit.
    Pfami PF00322. Endothelin. 1 hit.
    [Graphical view ]
    PRINTSi PR00365. ENDOTHELIN.
    SMARTi SM00272. END. 2 hits.
    [Graphical view ]
    PROSITEi PS00270. ENDOTHELIN. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Specific expression of human endothelin-2 (ET-2) gene in a renal adenocarcinoma cell line. Molecular cloning of cDNA encoding the precursor of ET-2 and its characterization."
      Ohkubo S., Ogi K., Hosoya M., Matsumoto H., Suzuki N., Kimura C., Onda H., Fujino M.
      FEBS Lett. 274:136-140(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "cDNA cloning and chromosomal assignment of the endothelin 2 gene: vasoactive intestinal contractor peptide is rat endothelin 2."
      Bloch K.D., Hong C.C., Eddy R.L. Jr., Shows T.B., Quertermous T.
      Genomics 10:236-242(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Expression of endothelin-2 (ET-2) gene in a human renal adenocarcinoma cell line: purification and cDNA cloning of ET-2."
      Onda H., Ohkubo S., Kosaka T., Yasuhara T., Ogi K., Hosoya M., Matsumoto H., Suzuki N., Kitada C., Ishibashi Y., Kimura C., Kubo K., Fujino M.
      J. Cardiovasc. Pharmacol. 17:S39-S43(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. NIEHS SNPs program
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LEU-168.
    5. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon, Kidney and Stomach.
    8. "The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes."
      Inoue A., Yanagisawa M., Kimura S., Kasuya Y., Miyauchi T., Goto K., Masaki T.
      Proc. Natl. Acad. Sci. U.S.A. 86:2863-2867(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-73.
    9. "Proteolytic processing of big endothelin-3 by the kell blood group protein."
      Lee S., Lin M., Mele A., Cao Y., Farmar J., Russo D., Redman C.
      Blood 94:1440-1450(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, CLEAVAGE BY KELL, IDENTIFICATION BY MASS SPECTROMETRY.
    10. "Endothelin-1 and ETA receptor expression in vascular smooth muscle cells from human placenta: a new ETA receptor messenger ribonucleic acid is generated by alternative splicing of exon 3."
      Bourgeois C., Robert B., Rebourcet R., Mondon F., Mignot T.-M., Duc-Goiran P., Ferre F.
      J. Clin. Endocrinol. Metab. 82:3116-3123(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiEDN2_HUMAN
    AccessioniPrimary (citable) accession number: P20800
    Secondary accession number(s): Q5T1R3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 140 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3