Vacuolar protein sorting-associated protein 33 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P20795 (VPS33_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 33

Alternative name(s):
Protein SLP1
Vacuolar morphogenesis protein 5

Gene names

Name:	VPS33
Synonyms:	SLP1, VAM5
Ordered Locus Names:	YLR396C
ORF Names:	L8084.15

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for vacuolar biogenesis, maturation and function. Involved in the sorting of vacuolar proteins from the Golgi apparatus and their targeting to the vacuole. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion. Ref.7
Subunit structure	Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with IVY1 and VAM7. Ref.5 Ref.7
Subcellular location	Vacuole.
Miscellaneous	Present with 830 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the STXBP/unc-18/SEC1 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Vacuole
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to vacuole transport Inferred from mutant phenotype Ref.1. Source: SGD endocytosis Inferred from mutant phenotype. Source: SGD piecemeal microautophagy of nucleus Inferred from mutant phenotype. Source: SGD protein targeting to vacuole Inferred from mutant phenotype. Source: SGD regulation of SNARE complex assembly Inferred from direct assay. Source: SGD regulation of vacuole fusion, non-autophagic Inferred from direct assay. Source: SGD vacuole fusion, non-autophagic Inferred from mutant phenotype. Source: SGD vesicle docking involved in exocytosis Inferred from electronic annotation. Source: InterPro vesicle fusion with vacuole Inferred from genetic interaction. Source: SGD
Cellular component	CORVET complex Inferred from direct assay. Source: SGD cytosol Inferred from direct assay. Source: SGD fungal-type vacuole membrane Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction Ref.5. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IVY1	Q04934	3	EBI-20395,EBI-35255

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 691

691

Vacuolar protein sorting-associated protein 33

PRO_0000206311

Amino acid modifications

Modified residue

373

Phosphoserine Ref.8

Modified residue

626

Phosphoserine Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P20795 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: ED78DF55109C20E7
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FASTA

691

79,271

        10         20         30         40         50         60 
MNRFWNTKKF SLTNADGLCA TLNEISQNDE VLVVQPSVLP VLNSLLTFQD LTQSTPVRKI 

        70         80         90        100        110        120 
TLLDDQLSDD LPSALGSVPQ MDLIFLIDVR TSLRLPPQLL DAAQKHNLSS LHIIYCRWKP 

       130        140        150        160        170        180 
SFQNTLEDTE QWQKDGFDLN SKKTHFPNVI ESQLKELSNE YTLYPWDLLP FPQIDENVLL 

       190        200        210        220        230        240 
THSLYNMENV NMYYPNLRSL QSATESILVD DMVNSLQSLI FETNSIITNV VSIGNLSKRC 

       250        260        270        280        290        300 
SHLLKKRIDE HQTENDLFIK GTLYGERTNC GLEMDLIILE RNTDPITPLL TQLTYAGILD 

       310        320        330        340        350        360 
DLYEFNSGIK IKEKDMNFNY KEDKIWNDLK FLNFGSIGPQ LNKLAKELQT QYDTRHKAES 

       370        380        390        400        410        420 
VHEIKEFVDS LGSLQQRQAF LKNHTTLSSD VLKVVETEEY GSFNKILELE LEILMGNTLN 

       430        440        450        460        470        480 
NDIEDIILEL QYQYEVDQKK ILRLICLLSL CKNSLREKDY EYLRTFMIDS WGIEKCFQLE 

       490        500        510        520        530        540 
SLAELGFFTS KTGKTDLHIT TSKSTRLQKE YRYISQWFNT VPIEDEHAAD KITNENDDFS 

       550        560        570        580        590        600 
EATFAYSGVV PLTMRLVQML YDRSILFHNY SSQQPFILSR EPRVSQTEDL IEQLYGDSHA 

       610        620        630        640        650        660 
IEESIWVPGT ITKKINASIK SNNRRSIDGS NGTFHAAEDI ALVVFLGGVT MGEIAIMKHL 

       670        680        690 
QKILGKKGIN KRFIIIADGL INGTRIMNSI S

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The SLP1 gene of Saccharomyces cerevisiae is essential for vacuolar morphogenesis and function." Wada Y., Kitamoto K., Kanbe T., Tanaka K., Anraku Y. Mol. Cell. Biol. 10:2214-2223(1990) [PubMed: 2183024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of yeast Vps33p, a protein required for vacuolar protein sorting and vacuole biogenesis." Banta L.M., Vida T.A., Herman P.K., Emr S.D. Mol. Cell. Biol. 10:4638-4649(1990) [PubMed: 2201898] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"A novel phospholipid-binding protein from the yeast Saccharomyces cerevisiae with dual binding specificities for the transport GTPase Ypt7p and the Sec1-related Vps33p." Lazar T., Scheglmann D., Gallwitz D. Eur. J. Cell Biol. 81:635-646(2002) [PubMed: 12553664] [Abstract] Cited for: INTERACTION WITH IVY1.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p." Stroupe C., Collins K.M., Fratti R.A., Wickner W. EMBO J. 25:1579-1589(2006) [PubMed: 16601699] [Abstract] Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-373 AND SER-626, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34474 mRNA. Translation: AAA35052.1. M34638 Genomic DNA. Translation: AAA35217.1. U19729 Genomic DNA. Translation: AAB82354.1. BK006945 Genomic DNA. Translation: DAA09697.1.
PIR	A34708.
RefSeq	NP_013500.1. NM_001182284.1.
3D structure databases
ProteinModelPortal	P20795.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5910N.
IntAct	P20795. 13 interactions.
MINT	MINT-615203.
STRING	P20795.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR396C; YLR396C; YLR396C.
GeneID	851112.
KEGG	sce:YLR396C.
NMPDR	fig\|4932.3.peg.4525.
Organism-specific databases
CYGD	YLR396c.
SGD	S000004388. VPS33.
Phylogenomic databases
eggNOG	fuNOG07738.
GeneTree	EFGT00050000003490.
HOGENOM	HBG202575.
OMA	NFGSIGP.
OrthoDB	EOG4ZKNW2.
Gene expression databases
ArrayExpress	P20795.
Genevestigator	P20795.
GermOnline	YLR396C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001619. Sec1-like. [Graphical view]
PANTHER	PTHR11679. Sec1-like. 1 hit.
Pfam	PF00995. Sec1. 1 hit. [Graphical view]
SUPFAM	SSF56815. Sec1-like. 1 hit.
ProtoNet	Search...
Other
NextBio	967824.

Entry information

Entry name

VPS33_YEAST

Accession

Primary (citable) accession number: P20795
Secondary accession number(s): D6VZ31

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	January 25, 2012
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents