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P20795 (VPS33_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein sorting-associated protein 33
Alternative name(s):
Protein SLP1
Vacuolar morphogenesis protein 5
Gene names
Name:VPS33
Synonyms:SLP1, VAM5
Ordered Locus Names:YLR396C
ORF Names:L8084.15
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length691 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for vacuolar biogenesis, maturation and function. Involved in the sorting of vacuolar proteins from the Golgi apparatus and their targeting to the vacuole. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion. Ref.7

Subunit structure

Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with IVY1 and VAM7. Ref.5 Ref.7

Subcellular location

Vacuole.

Miscellaneous

Present with 830 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the STXBP/unc-18/SEC1 family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentVacuole
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGolgi to vacuole transport

Inferred from mutant phenotype PubMed 15047864Ref.1. Source: SGD

endocytosis

Inferred from mutant phenotype PubMed 15047864. Source: SGD

piecemeal microautophagy of nucleus

Inferred from mutant phenotype PubMed 18701704. Source: SGD

protein targeting to vacuole

Inferred from mutant phenotype PubMed 3062374. Source: SGD

regulation of SNARE complex assembly

Inferred from direct assay PubMed 18385512. Source: SGD

regulation of vacuole fusion, non-autophagic

Inferred from direct assay PubMed 18385512. Source: SGD

vacuole fusion, non-autophagic

Inferred from mutant phenotype PubMed 10944212. Source: SGD

vacuole organization

Inferred from mutant phenotype Ref.1. Source: SGD

vesicle docking

Inferred from genetic interaction PubMed 9245783. Source: SGD

vesicle docking involved in exocytosis

Inferred from electronic annotation. Source: InterPro

vesicle fusion with vacuole

Inferred from genetic interaction PubMed 9245783. Source: SGD

   Cellular_componentCORVET complex

Inferred from direct assay PubMed 17488625. Source: SGD

HOPS complex

Inferred from physical interaction PubMed 10944212. Source: SGD

cytosol

Inferred from direct assay PubMed 9624182. Source: SGD

fungal-type vacuole

Inferred from direct assay PubMed 11853670. Source: SGD

fungal-type vacuole membrane

Inferred from direct assay PubMed 9624182. Source: SGD

   Molecular_functionATP binding

Inferred from direct assay PubMed 9624182. Source: SGD

protein binding

Inferred from physical interaction PubMed 10944212PubMed 11805826Ref.5PubMed 16429126Ref.7. Source: IntAct

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 691691Vacuolar protein sorting-associated protein 33
PRO_0000206311

Amino acid modifications

Modified residue6261Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P20795 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: ED78DF55109C20E7

FASTA69179,271
        10         20         30         40         50         60 
MNRFWNTKKF SLTNADGLCA TLNEISQNDE VLVVQPSVLP VLNSLLTFQD LTQSTPVRKI 

        70         80         90        100        110        120 
TLLDDQLSDD LPSALGSVPQ MDLIFLIDVR TSLRLPPQLL DAAQKHNLSS LHIIYCRWKP 

       130        140        150        160        170        180 
SFQNTLEDTE QWQKDGFDLN SKKTHFPNVI ESQLKELSNE YTLYPWDLLP FPQIDENVLL 

       190        200        210        220        230        240 
THSLYNMENV NMYYPNLRSL QSATESILVD DMVNSLQSLI FETNSIITNV VSIGNLSKRC 

       250        260        270        280        290        300 
SHLLKKRIDE HQTENDLFIK GTLYGERTNC GLEMDLIILE RNTDPITPLL TQLTYAGILD 

       310        320        330        340        350        360 
DLYEFNSGIK IKEKDMNFNY KEDKIWNDLK FLNFGSIGPQ LNKLAKELQT QYDTRHKAES 

       370        380        390        400        410        420 
VHEIKEFVDS LGSLQQRQAF LKNHTTLSSD VLKVVETEEY GSFNKILELE LEILMGNTLN 

       430        440        450        460        470        480 
NDIEDIILEL QYQYEVDQKK ILRLICLLSL CKNSLREKDY EYLRTFMIDS WGIEKCFQLE 

       490        500        510        520        530        540 
SLAELGFFTS KTGKTDLHIT TSKSTRLQKE YRYISQWFNT VPIEDEHAAD KITNENDDFS 

       550        560        570        580        590        600 
EATFAYSGVV PLTMRLVQML YDRSILFHNY SSQQPFILSR EPRVSQTEDL IEQLYGDSHA 

       610        620        630        640        650        660 
IEESIWVPGT ITKKINASIK SNNRRSIDGS NGTFHAAEDI ALVVFLGGVT MGEIAIMKHL 

       670        680        690 
QKILGKKGIN KRFIIIADGL INGTRIMNSI S 

« Hide

References

« Hide 'large scale' references
[1]"The SLP1 gene of Saccharomyces cerevisiae is essential for vacuolar morphogenesis and function."
Wada Y., Kitamoto K., Kanbe T., Tanaka K., Anraku Y.
Mol. Cell. Biol. 10:2214-2223(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Characterization of yeast Vps33p, a protein required for vacuolar protein sorting and vacuole biogenesis."
Banta L.M., Vida T.A., Herman P.K., Emr S.D.
Mol. Cell. Biol. 10:4638-4649(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"A novel phospholipid-binding protein from the yeast Saccharomyces cerevisiae with dual binding specificities for the transport GTPase Ypt7p and the Sec1-related Vps33p."
Lazar T., Scheglmann D., Gallwitz D.
Eur. J. Cell Biol. 81:635-646(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IVY1.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p."
Stroupe C., Collins K.M., Fratti R.A., Wickner W.
EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-626, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34474 mRNA. Translation: AAA35052.1.
M34638 Genomic DNA. Translation: AAA35217.1.
U19729 Genomic DNA. Translation: AAB82354.1.
BK006945 Genomic DNA. Translation: DAA09697.1.
PIRA34708.
RefSeqNP_013500.3. NM_001182284.3.

3D structure databases

ProteinModelPortalP20795.
SMRP20795. Positions 220-690.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31655. 70 interactions.
DIPDIP-5910N.
IntActP20795. 14 interactions.
MINTMINT-615203.
STRING4932.YLR396C.

Proteomic databases

MaxQBP20795.
PaxDbP20795.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR396C; YLR396C; YLR396C.
GeneID851112.
KEGGsce:YLR396C.

Organism-specific databases

CYGDYLR396c.
SGDS000004388. VPS33.

Phylogenomic databases

eggNOGCOG5158.
GeneTreeENSGT00530000063488.
HOGENOMHOG000001143.
OMAAIEESIW.
OrthoDBEOG7N37PR.

Enzyme and pathway databases

BioCycYEAST:G3O-32460-MONOMER.

Gene expression databases

GenevestigatorP20795.

Family and domain databases

Gene3D3.40.50.1910. 3 hits.
InterProIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
IPR027121. VPS33.
[Graphical view]
PANTHERPTHR11679. PTHR11679. 1 hit.
PTHR11679:SF1. PTHR11679:SF1. 1 hit.
PfamPF00995. Sec1. 1 hit.
[Graphical view]
SUPFAMSSF56815. SSF56815. 3 hits.
ProtoNetSearch...

Other

NextBio967824.
PROP20795.

Entry information

Entry nameVPS33_YEAST
AccessionPrimary (citable) accession number: P20795
Secondary accession number(s): D6VZ31
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 11, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families