Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar protein sorting-associated protein 33

Gene

VPS33

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for vacuolar biogenesis, maturation and function. Involved in the sorting of vacuolar proteins from the Golgi apparatus and their targeting to the vacuole. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion.1 Publication

Miscellaneous

Present with 830 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • ATP binding Source: SGD

GO - Biological processi

  • endocytosis Source: SGD
  • Golgi to vacuole transport Source: SGD
  • piecemeal microautophagy of the nucleus Source: SGD
  • protein targeting to vacuole Source: SGD
  • regulation of SNARE complex assembly Source: SGD
  • regulation of vacuole fusion, non-autophagic Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
  • vacuole organization Source: SGD
  • vesicle docking Source: SGD
  • vesicle docking involved in exocytosis Source: InterPro
  • vesicle fusion with vacuole Source: SGD
  • vesicle tethering Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32460-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 33
Alternative name(s):
Protein SLP1
Vacuolar morphogenesis protein 5
Gene namesi
Name:VPS33
Synonyms:SLP1, VAM5
Ordered Locus Names:YLR396C
ORF Names:L8084.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR396C
SGDiS000004388 VPS33

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002063111 – 691Vacuolar protein sorting-associated protein 33Add BLAST691

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei626PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP20795
PaxDbiP20795
PRIDEiP20795

PTM databases

iPTMnetiP20795

Interactioni

Subunit structurei

Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with IVY1 and VAM7.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31655, 78 interactors
DIPiDIP-5910N
IntActiP20795, 19 interactors
MINTiP20795
STRINGi4932.YLR396C

Structurei

3D structure databases

ProteinModelPortaliP20795
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the STXBP/unc-18/SEC1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063488
HOGENOMiHOG000001143
InParanoidiP20795
KOiK20182
OMAiKFWNLHP
OrthoDBiEOG092C1RCH

Family and domain databases

Gene3Di3.40.50.1910, 3 hits
InterProiView protein in InterPro
IPR027482 Sec-1-like_dom2
IPR001619 Sec1-like
IPR036045 Sec1-like_sf
IPR027121 VPS33
PANTHERiPTHR11679 PTHR11679, 1 hit
PTHR11679:SF1 PTHR11679:SF1, 1 hit
PfamiView protein in Pfam
PF00995 Sec1, 1 hit
SUPFAMiSSF56815 SSF56815, 3 hits

Sequencei

Sequence statusi: Complete.

P20795-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRFWNTKKF SLTNADGLCA TLNEISQNDE VLVVQPSVLP VLNSLLTFQD
60 70 80 90 100
LTQSTPVRKI TLLDDQLSDD LPSALGSVPQ MDLIFLIDVR TSLRLPPQLL
110 120 130 140 150
DAAQKHNLSS LHIIYCRWKP SFQNTLEDTE QWQKDGFDLN SKKTHFPNVI
160 170 180 190 200
ESQLKELSNE YTLYPWDLLP FPQIDENVLL THSLYNMENV NMYYPNLRSL
210 220 230 240 250
QSATESILVD DMVNSLQSLI FETNSIITNV VSIGNLSKRC SHLLKKRIDE
260 270 280 290 300
HQTENDLFIK GTLYGERTNC GLEMDLIILE RNTDPITPLL TQLTYAGILD
310 320 330 340 350
DLYEFNSGIK IKEKDMNFNY KEDKIWNDLK FLNFGSIGPQ LNKLAKELQT
360 370 380 390 400
QYDTRHKAES VHEIKEFVDS LGSLQQRQAF LKNHTTLSSD VLKVVETEEY
410 420 430 440 450
GSFNKILELE LEILMGNTLN NDIEDIILEL QYQYEVDQKK ILRLICLLSL
460 470 480 490 500
CKNSLREKDY EYLRTFMIDS WGIEKCFQLE SLAELGFFTS KTGKTDLHIT
510 520 530 540 550
TSKSTRLQKE YRYISQWFNT VPIEDEHAAD KITNENDDFS EATFAYSGVV
560 570 580 590 600
PLTMRLVQML YDRSILFHNY SSQQPFILSR EPRVSQTEDL IEQLYGDSHA
610 620 630 640 650
IEESIWVPGT ITKKINASIK SNNRRSIDGS NGTFHAAEDI ALVVFLGGVT
660 670 680 690
MGEIAIMKHL QKILGKKGIN KRFIIIADGL INGTRIMNSI S
Length:691
Mass (Da):79,271
Last modified:February 1, 1991 - v1
Checksum:iED78DF55109C20E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34474 mRNA Translation: AAA35052.1
M34638 Genomic DNA Translation: AAA35217.1
U19729 Genomic DNA Translation: AAB82354.1
BK006945 Genomic DNA Translation: DAA09697.1
PIRiA34708
RefSeqiNP_013500.3, NM_001182284.3

Genome annotation databases

EnsemblFungiiYLR396C; YLR396C; YLR396C
GeneIDi851112
KEGGisce:YLR396C

Similar proteinsi

Entry informationi

Entry nameiVPS33_YEAST
AccessioniPrimary (citable) accession number: P20795
Secondary accession number(s): D6VZ31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 23, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health