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P20795

- VPS33_YEAST

UniProt

P20795 - VPS33_YEAST

Protein

Vacuolar protein sorting-associated protein 33

Gene

VPS33

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Essential for vacuolar biogenesis, maturation and function. Involved in the sorting of vacuolar proteins from the Golgi apparatus and their targeting to the vacuole. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion.1 Publication

    GO - Molecular functioni

    1. ATP binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. endocytosis Source: SGD
    2. Golgi to vacuole transport Source: SGD
    3. piecemeal microautophagy of nucleus Source: SGD
    4. protein targeting to vacuole Source: SGD
    5. regulation of SNARE complex assembly Source: SGD
    6. regulation of vacuole fusion, non-autophagic Source: SGD
    7. vacuole fusion, non-autophagic Source: SGD
    8. vacuole organization Source: SGD
    9. vesicle docking Source: SGD
    10. vesicle docking involved in exocytosis Source: InterPro
    11. vesicle fusion with vacuole Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32460-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein sorting-associated protein 33
    Alternative name(s):
    Protein SLP1
    Vacuolar morphogenesis protein 5
    Gene namesi
    Name:VPS33
    Synonyms:SLP1, VAM5
    Ordered Locus Names:YLR396C
    ORF Names:L8084.15
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR396c.
    SGDiS000004388. VPS33.

    Subcellular locationi

    GO - Cellular componenti

    1. CORVET complex Source: SGD
    2. cytosol Source: SGD
    3. fungal-type vacuole Source: SGD
    4. fungal-type vacuole membrane Source: SGD
    5. HOPS complex Source: SGD

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 691691Vacuolar protein sorting-associated protein 33PRO_0000206311Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei626 – 6261Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP20795.
    PaxDbiP20795.

    Expressioni

    Gene expression databases

    GenevestigatoriP20795.

    Interactioni

    Subunit structurei

    Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with IVY1 and VAM7.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    IVY1Q049343EBI-20395,EBI-35255
    PEP3P278017EBI-20395,EBI-13130
    PEP5P128685EBI-20395,EBI-6450
    VAM6Q074684EBI-20395,EBI-20422
    VPS16Q033085EBI-20395,EBI-20355
    VPS41P389595EBI-20395,EBI-20432

    Protein-protein interaction databases

    BioGridi31655. 70 interactions.
    DIPiDIP-5910N.
    IntActiP20795. 14 interactions.
    MINTiMINT-615203.
    STRINGi4932.YLR396C.

    Structurei

    3D structure databases

    ProteinModelPortaliP20795.
    SMRiP20795. Positions 220-690.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the STXBP/unc-18/SEC1 family.Curated

    Phylogenomic databases

    eggNOGiCOG5158.
    GeneTreeiENSGT00530000063488.
    HOGENOMiHOG000001143.
    OMAiAIEESIW.
    OrthoDBiEOG7N37PR.

    Family and domain databases

    Gene3Di3.40.50.1910. 3 hits.
    InterProiIPR027482. Sec-1-like_dom2.
    IPR001619. Sec1-like.
    IPR027121. VPS33.
    [Graphical view]
    PANTHERiPTHR11679. PTHR11679. 1 hit.
    PTHR11679:SF1. PTHR11679:SF1. 1 hit.
    PfamiPF00995. Sec1. 1 hit.
    [Graphical view]
    SUPFAMiSSF56815. SSF56815. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    P20795-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRFWNTKKF SLTNADGLCA TLNEISQNDE VLVVQPSVLP VLNSLLTFQD    50
    LTQSTPVRKI TLLDDQLSDD LPSALGSVPQ MDLIFLIDVR TSLRLPPQLL 100
    DAAQKHNLSS LHIIYCRWKP SFQNTLEDTE QWQKDGFDLN SKKTHFPNVI 150
    ESQLKELSNE YTLYPWDLLP FPQIDENVLL THSLYNMENV NMYYPNLRSL 200
    QSATESILVD DMVNSLQSLI FETNSIITNV VSIGNLSKRC SHLLKKRIDE 250
    HQTENDLFIK GTLYGERTNC GLEMDLIILE RNTDPITPLL TQLTYAGILD 300
    DLYEFNSGIK IKEKDMNFNY KEDKIWNDLK FLNFGSIGPQ LNKLAKELQT 350
    QYDTRHKAES VHEIKEFVDS LGSLQQRQAF LKNHTTLSSD VLKVVETEEY 400
    GSFNKILELE LEILMGNTLN NDIEDIILEL QYQYEVDQKK ILRLICLLSL 450
    CKNSLREKDY EYLRTFMIDS WGIEKCFQLE SLAELGFFTS KTGKTDLHIT 500
    TSKSTRLQKE YRYISQWFNT VPIEDEHAAD KITNENDDFS EATFAYSGVV 550
    PLTMRLVQML YDRSILFHNY SSQQPFILSR EPRVSQTEDL IEQLYGDSHA 600
    IEESIWVPGT ITKKINASIK SNNRRSIDGS NGTFHAAEDI ALVVFLGGVT 650
    MGEIAIMKHL QKILGKKGIN KRFIIIADGL INGTRIMNSI S 691
    Length:691
    Mass (Da):79,271
    Last modified:February 1, 1991 - v1
    Checksum:iED78DF55109C20E7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34474 mRNA. Translation: AAA35052.1.
    M34638 Genomic DNA. Translation: AAA35217.1.
    U19729 Genomic DNA. Translation: AAB82354.1.
    BK006945 Genomic DNA. Translation: DAA09697.1.
    PIRiA34708.
    RefSeqiNP_013500.3. NM_001182284.3.

    Genome annotation databases

    EnsemblFungiiYLR396C; YLR396C; YLR396C.
    GeneIDi851112.
    KEGGisce:YLR396C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34474 mRNA. Translation: AAA35052.1 .
    M34638 Genomic DNA. Translation: AAA35217.1 .
    U19729 Genomic DNA. Translation: AAB82354.1 .
    BK006945 Genomic DNA. Translation: DAA09697.1 .
    PIRi A34708.
    RefSeqi NP_013500.3. NM_001182284.3.

    3D structure databases

    ProteinModelPortali P20795.
    SMRi P20795. Positions 220-690.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31655. 70 interactions.
    DIPi DIP-5910N.
    IntActi P20795. 14 interactions.
    MINTi MINT-615203.
    STRINGi 4932.YLR396C.

    Proteomic databases

    MaxQBi P20795.
    PaxDbi P20795.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR396C ; YLR396C ; YLR396C .
    GeneIDi 851112.
    KEGGi sce:YLR396C.

    Organism-specific databases

    CYGDi YLR396c.
    SGDi S000004388. VPS33.

    Phylogenomic databases

    eggNOGi COG5158.
    GeneTreei ENSGT00530000063488.
    HOGENOMi HOG000001143.
    OMAi AIEESIW.
    OrthoDBi EOG7N37PR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32460-MONOMER.

    Miscellaneous databases

    NextBioi 967824.
    PROi P20795.

    Gene expression databases

    Genevestigatori P20795.

    Family and domain databases

    Gene3Di 3.40.50.1910. 3 hits.
    InterProi IPR027482. Sec-1-like_dom2.
    IPR001619. Sec1-like.
    IPR027121. VPS33.
    [Graphical view ]
    PANTHERi PTHR11679. PTHR11679. 1 hit.
    PTHR11679:SF1. PTHR11679:SF1. 1 hit.
    Pfami PF00995. Sec1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56815. SSF56815. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The SLP1 gene of Saccharomyces cerevisiae is essential for vacuolar morphogenesis and function."
      Wada Y., Kitamoto K., Kanbe T., Tanaka K., Anraku Y.
      Mol. Cell. Biol. 10:2214-2223(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Characterization of yeast Vps33p, a protein required for vacuolar protein sorting and vacuole biogenesis."
      Banta L.M., Vida T.A., Herman P.K., Emr S.D.
      Mol. Cell. Biol. 10:4638-4649(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "A novel phospholipid-binding protein from the yeast Saccharomyces cerevisiae with dual binding specificities for the transport GTPase Ypt7p and the Sec1-related Vps33p."
      Lazar T., Scheglmann D., Gallwitz D.
      Eur. J. Cell Biol. 81:635-646(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH IVY1.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p."
      Stroupe C., Collins K.M., Fratti R.A., Wickner W.
      EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-626, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiVPS33_YEAST
    AccessioniPrimary (citable) accession number: P20795
    Secondary accession number(s): D6VZ31
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 830 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3