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P20793

- MAK_RAT

UniProt

P20793 - MAK_RAT

Protein

Serine/threonine-protein kinase MAK

Gene

Mak

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (21 Mar 2012)
      Previous versions | rss
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    Functioni

    Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors By similarity. Could play an important function in spermatogenesis. Phosphorylates FZR1 in a cell cycle-dependent manner By similarity. Plays a role in the transcriptional coactivation of AR By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei125 – 1251Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    3. transcription coactivator activity Source: UniProtKB

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. multicellular organismal development Source: UniProtKB-KW
    3. photoreceptor cell maintenance Source: UniProtKB
    4. protein autophosphorylation Source: UniProtKB
    5. protein phosphorylation Source: UniProtKB
    6. regulation of transcription, DNA-templated Source: UniProtKB-KW
    7. spermatogenesis Source: UniProtKB-KW
    8. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Differentiation, Spermatogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase MAK (EC:2.7.11.22)
    Alternative name(s):
    Male germ cell-associated kinase
    Gene namesi
    Name:Mak
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 17

    Organism-specific databases

    RGDi3036. Mak.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm 1 Publication. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle By similarity. Midbody By similarity. Cell projectionciliumphotoreceptor outer segment By similarity. Photoreceptor inner segment By similarity
    Note: Localized in both the connecting cilia and the outer segment axonemes.By similarity

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. cytoplasm Source: UniProtKB-SubCell
    3. midbody Source: UniProtKB
    4. mitotic spindle Source: UniProtKB
    5. nucleus Source: UniProtKB
    6. photoreceptor inner segment Source: UniProtKB
    7. photoreceptor outer segment Source: UniProtKB

    Keywords - Cellular componenti

    Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Serine/threonine-protein kinase MAKPRO_0000086286Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei157 – 1571Phosphothreonine; by autocatalysisBy similarity
    Modified residuei159 – 1591Phosphotyrosine; by autocatalysisBy similarity

    Post-translational modificationi

    Autophosphorylated By similarity. Phosphorylated on serine and threonine residues.By similarity1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP20793.
    PRIDEiP20793.

    Expressioni

    Tissue specificityi

    Expressed mainly in testicular cells at and after meiosis.2 Publications

    Developmental stagei

    Expression in testis is detected 16 days after birth and increases gradually to reach a plateau about 4 weeks after birth (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriP20793.

    Interactioni

    Subunit structurei

    Interacts with RP1. Interacts with AR and CDK20. Found in a complex containing MAK, AR and NCOA3. Interacts with FZR1 (via WD repeats) By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000059085.

    Structurei

    3D structure databases

    ProteinModelPortaliP20793.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 284281Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi309 – 36860Glu/Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00650000093283.
    HOGENOMiHOG000233024.
    HOVERGENiHBG014652.
    InParanoidiP20793.
    KOiK08829.
    OMAiYATYNQS.
    OrthoDBiEOG7NCV37.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P20793-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNRYTTMRQL GDGTYGSVLM GKSNESGELV AIKRMKRKFY SWDECMNLRE    50
    VKSLKKLNHA NVIKLKEVIR ENDHLYFIFE YMKENLYQLM KDRNKLFPES 100
    VIRNIMYQIL QGLAFIHKHG FFHRDMKPEN LLCMGPELVK IADFGLAREL 150
    RSQPPYTDYV STRWYRAPEV LLRSSVYSSP IDVWAVGSIM AELYTFRPLF 200
    PGTSEVDEIF KICQVLGTPK KSDWPEGYQL ASSMNFRFPQ CIPINLKTLI 250
    PNASSEAIQL MTEMLNWDPK KRPTASQALK HPYFQVGQVL GPSAHHLDAK 300
    QTLHKQLQPP EPKPSSSERD PKPLPNILDQ PAGQPQPKQG HQPLQAIQPP 350
    QNTVVQPPPK QQGHHKQPQT MFPSIVKTIP TNPVSTVGHK GARRRWGQTV 400
    FKSGDSCDNI EDCDLGASHS KKPSMDAFKE KKKKESPFRF PEAGLPVSNH 450
    LKGENRNLHA SLKSDTNLST ASTAKQYYLK QSRYLPGVNP KNVSLVAGGK 500
    DINSHSWNNQ LFPKSLGSMG ADLAFKRSNA AGNLGSYSAY SQTGCVPSFL 550
    KKEVGSAGQR IHLAPLGASA ADYTWSTKTG QGQFSGRTYN PTAKNLNIVN 600
    RTQPVPSVHG RTDWVAKYGG HR 622
    Length:622
    Mass (Da):69,897
    Last modified:March 21, 2012 - v2
    Checksum:i7C2056E1D116D3BF
    GO
    Isoform 2 (identifier: P20793-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         531-571: Missing.

    Show »
    Length:581
    Mass (Da):65,849
    Checksum:i8F4911C903FF3B3D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti581 – 5811Q → R in AAA41562. (PubMed:2183027)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei531 – 57141Missing in isoform 2. 1 PublicationVSP_042475Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35862 mRNA. Translation: AAA41562.1.
    CH473977 Genomic DNA. Translation: EDL98222.1.
    BC078887 mRNA. Translation: AAH78887.1.
    PIRiA34711.
    RefSeqiNP_037268.1. NM_013136.1.
    UniGeneiRn.9670.

    Genome annotation databases

    EnsembliENSRNOT00000020672; ENSRNOP00000020672; ENSRNOG00000015101. [P20793-1]
    ENSRNOT00000040707; ENSRNOP00000050970; ENSRNOG00000015101. [P20793-2]
    GeneIDi25677.
    KEGGirno:25677.
    UCSCiRGD:3036. rat. [P20793-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35862 mRNA. Translation: AAA41562.1 .
    CH473977 Genomic DNA. Translation: EDL98222.1 .
    BC078887 mRNA. Translation: AAH78887.1 .
    PIRi A34711.
    RefSeqi NP_037268.1. NM_013136.1.
    UniGenei Rn.9670.

    3D structure databases

    ProteinModelPortali P20793.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000059085.

    Proteomic databases

    PaxDbi P20793.
    PRIDEi P20793.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000020672 ; ENSRNOP00000020672 ; ENSRNOG00000015101 . [P20793-1 ]
    ENSRNOT00000040707 ; ENSRNOP00000050970 ; ENSRNOG00000015101 . [P20793-2 ]
    GeneIDi 25677.
    KEGGi rno:25677.
    UCSCi RGD:3036. rat. [P20793-1 ]

    Organism-specific databases

    CTDi 4117.
    RGDi 3036. Mak.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00650000093283.
    HOGENOMi HOG000233024.
    HOVERGENi HBG014652.
    InParanoidi P20793.
    KOi K08829.
    OMAi YATYNQS.
    OrthoDBi EOG7NCV37.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 5301.

    Miscellaneous databases

    NextBioi 607631.
    PROi P20793.

    Gene expression databases

    Genevestigatori P20793.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel mammalian protein kinase gene (mak) is highly expressed in testicular germ cells at and after meiosis."
      Matsushime H., Jinno A., Takagi N., Shibuya M.
      Mol. Cell. Biol. 10:2261-2268(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Testis.
    2. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
      Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
      , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
      Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: Brown Norway.
      Tissue: Testis.
    4. "Testis-specific mak protein kinase is expressed specifically in the meiotic phase in spermatogenesis and is associated with a 210-kilodalton cellular phosphoprotein."
      Jinno A., Tanaka K., Matsushime H., Haneji T., Shibuya M.
      Mol. Cell. Biol. 13:4146-4156(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION, ALTERNATIVE SPLICING.

    Entry informationi

    Entry nameiMAK_RAT
    AccessioniPrimary (citable) accession number: P20793
    Secondary accession number(s): G3V7Y4, Q6AYW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: March 21, 2012
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3