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P20793

- MAK_RAT

UniProt

P20793 - MAK_RAT

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Protein

Serine/threonine-protein kinase MAK

Gene

Mak

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Could play an important function in spermatogenesis. Phosphorylates FZR1 in a cell cycle-dependent manner (By similarity). Plays a role in the transcriptional coactivation of AR (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei125 – 1251Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. transcription coactivator activity Source: UniProtKB

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. multicellular organismal development Source: UniProtKB-KW
  3. photoreceptor cell maintenance Source: UniProtKB
  4. protein autophosphorylation Source: UniProtKB
  5. protein phosphorylation Source: UniProtKB
  6. regulation of transcription, DNA-templated Source: UniProtKB-KW
  7. spermatogenesis Source: UniProtKB-KW
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase MAK (EC:2.7.11.22)
Alternative name(s):
Male germ cell-associated kinase
Gene namesi
Name:Mak
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 17

Organism-specific databases

RGDi3036. Mak.

Subcellular locationi

Nucleus By similarity. Cytoplasm 1 Publication. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle By similarity. Midbody By similarity. Cell projectionciliumphotoreceptor outer segment By similarity. Photoreceptor inner segment By similarity
Note: Localized in both the connecting cilia and the outer segment axonemes.By similarity

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
  3. midbody Source: UniProtKB
  4. mitotic spindle Source: UniProtKB
  5. nucleus Source: UniProtKB
  6. photoreceptor inner segment Source: UniProtKB
  7. photoreceptor outer segment Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Serine/threonine-protein kinase MAKPRO_0000086286Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571Phosphothreonine; by autocatalysisBy similarity
Modified residuei159 – 1591Phosphotyrosine; by autocatalysisBy similarity

Post-translational modificationi

Autophosphorylated (By similarity). Phosphorylated on serine and threonine residues.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP20793.
PRIDEiP20793.

Expressioni

Tissue specificityi

Expressed mainly in testicular cells at and after meiosis.2 Publications

Developmental stagei

Expression in testis is detected 16 days after birth and increases gradually to reach a plateau about 4 weeks after birth (at protein level).1 Publication

Gene expression databases

GenevestigatoriP20793.

Interactioni

Subunit structurei

Interacts with RP1. Interacts with AR and CDK20. Found in a complex containing MAK, AR and NCOA3. Interacts with FZR1 (via WD repeats) (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000059085.

Structurei

3D structure databases

ProteinModelPortaliP20793.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 284281Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi309 – 36860Glu/Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00650000093283.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiP20793.
KOiK08829.
OMAiYATYNQS.
OrthoDBiEOG7NCV37.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P20793-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRYTTMRQL GDGTYGSVLM GKSNESGELV AIKRMKRKFY SWDECMNLRE
60 70 80 90 100
VKSLKKLNHA NVIKLKEVIR ENDHLYFIFE YMKENLYQLM KDRNKLFPES
110 120 130 140 150
VIRNIMYQIL QGLAFIHKHG FFHRDMKPEN LLCMGPELVK IADFGLAREL
160 170 180 190 200
RSQPPYTDYV STRWYRAPEV LLRSSVYSSP IDVWAVGSIM AELYTFRPLF
210 220 230 240 250
PGTSEVDEIF KICQVLGTPK KSDWPEGYQL ASSMNFRFPQ CIPINLKTLI
260 270 280 290 300
PNASSEAIQL MTEMLNWDPK KRPTASQALK HPYFQVGQVL GPSAHHLDAK
310 320 330 340 350
QTLHKQLQPP EPKPSSSERD PKPLPNILDQ PAGQPQPKQG HQPLQAIQPP
360 370 380 390 400
QNTVVQPPPK QQGHHKQPQT MFPSIVKTIP TNPVSTVGHK GARRRWGQTV
410 420 430 440 450
FKSGDSCDNI EDCDLGASHS KKPSMDAFKE KKKKESPFRF PEAGLPVSNH
460 470 480 490 500
LKGENRNLHA SLKSDTNLST ASTAKQYYLK QSRYLPGVNP KNVSLVAGGK
510 520 530 540 550
DINSHSWNNQ LFPKSLGSMG ADLAFKRSNA AGNLGSYSAY SQTGCVPSFL
560 570 580 590 600
KKEVGSAGQR IHLAPLGASA ADYTWSTKTG QGQFSGRTYN PTAKNLNIVN
610 620
RTQPVPSVHG RTDWVAKYGG HR
Length:622
Mass (Da):69,897
Last modified:March 21, 2012 - v2
Checksum:i7C2056E1D116D3BF
GO
Isoform 2 (identifier: P20793-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     531-571: Missing.

Show »
Length:581
Mass (Da):65,849
Checksum:i8F4911C903FF3B3D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti581 – 5811Q → R in AAA41562. (PubMed:2183027)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei531 – 57141Missing in isoform 2. 1 PublicationVSP_042475Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35862 mRNA. Translation: AAA41562.1.
CH473977 Genomic DNA. Translation: EDL98222.1.
BC078887 mRNA. Translation: AAH78887.1.
PIRiA34711.
RefSeqiNP_037268.1. NM_013136.1.
UniGeneiRn.9670.

Genome annotation databases

EnsembliENSRNOT00000020672; ENSRNOP00000020672; ENSRNOG00000015101. [P20793-1]
ENSRNOT00000040707; ENSRNOP00000050970; ENSRNOG00000015101. [P20793-2]
GeneIDi25677.
KEGGirno:25677.
UCSCiRGD:3036. rat. [P20793-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35862 mRNA. Translation: AAA41562.1 .
CH473977 Genomic DNA. Translation: EDL98222.1 .
BC078887 mRNA. Translation: AAH78887.1 .
PIRi A34711.
RefSeqi NP_037268.1. NM_013136.1.
UniGenei Rn.9670.

3D structure databases

ProteinModelPortali P20793.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000059085.

Proteomic databases

PaxDbi P20793.
PRIDEi P20793.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000020672 ; ENSRNOP00000020672 ; ENSRNOG00000015101 . [P20793-1 ]
ENSRNOT00000040707 ; ENSRNOP00000050970 ; ENSRNOG00000015101 . [P20793-2 ]
GeneIDi 25677.
KEGGi rno:25677.
UCSCi RGD:3036. rat. [P20793-1 ]

Organism-specific databases

CTDi 4117.
RGDi 3036. Mak.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00650000093283.
HOGENOMi HOG000233024.
HOVERGENi HBG014652.
InParanoidi P20793.
KOi K08829.
OMAi YATYNQS.
OrthoDBi EOG7NCV37.

Enzyme and pathway databases

BRENDAi 2.7.11.22. 5301.

Miscellaneous databases

NextBioi 607631.
PROi P20793.

Gene expression databases

Genevestigatori P20793.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel mammalian protein kinase gene (mak) is highly expressed in testicular germ cells at and after meiosis."
    Matsushime H., Jinno A., Takagi N., Shibuya M.
    Mol. Cell. Biol. 10:2261-2268(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Brown Norway.
    Tissue: Testis.
  4. "Testis-specific mak protein kinase is expressed specifically in the meiotic phase in spermatogenesis and is associated with a 210-kilodalton cellular phosphoprotein."
    Jinno A., Tanaka K., Matsushime H., Haneji T., Shibuya M.
    Mol. Cell. Biol. 13:4146-4156(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION, ALTERNATIVE SPLICING.

Entry informationi

Entry nameiMAK_RAT
AccessioniPrimary (citable) accession number: P20793
Secondary accession number(s): G3V7Y4, Q6AYW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: March 21, 2012
Last modified: October 29, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3