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Protein

Serine/threonine-protein kinase MAK

Gene

Mak

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Could play an important function in spermatogenesis. Phosphorylates FZR1 in a cell cycle-dependent manner (By similarity). Plays a role in the transcriptional coactivation of AR (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei125Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase MAK (EC:2.7.11.22)
Alternative name(s):
Male germ cell-associated kinase
Gene namesi
Name:Mak
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi3036. Mak.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000862861 – 622Serine/threonine-protein kinase MAKAdd BLAST622

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157Phosphothreonine; by autocatalysisBy similarity1
Modified residuei159Phosphotyrosine; by autocatalysisBy similarity1

Post-translational modificationi

Autophosphorylated (By similarity). Phosphorylated on serine and threonine residues.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP20793.
PRIDEiP20793.

PTM databases

PhosphoSitePlusiP20793.

Expressioni

Tissue specificityi

Expressed mainly in testicular cells at and after meiosis.2 Publications

Developmental stagei

Expression in testis is detected 16 days after birth and increases gradually to reach a plateau about 4 weeks after birth (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000015101.
GenevisibleiP20793. RN.

Interactioni

Subunit structurei

Interacts with RP1. Interacts with AR and CDK20. Found in a complex containing MAK, AR and NCOA3. Interacts with FZR1 (via WD repeats) (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020672.

Structurei

3D structure databases

ProteinModelPortaliP20793.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 284Protein kinasePROSITE-ProRule annotationAdd BLAST281

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi309 – 368Glu/Pro-richAdd BLAST60

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0661. Eukaryota.
ENOG410XPBB. LUCA.
GeneTreeiENSGT00650000093283.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiP20793.
KOiK08829.
OMAiYATYNQS.
OrthoDBiEOG091G0DU3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P20793-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRYTTMRQL GDGTYGSVLM GKSNESGELV AIKRMKRKFY SWDECMNLRE
60 70 80 90 100
VKSLKKLNHA NVIKLKEVIR ENDHLYFIFE YMKENLYQLM KDRNKLFPES
110 120 130 140 150
VIRNIMYQIL QGLAFIHKHG FFHRDMKPEN LLCMGPELVK IADFGLAREL
160 170 180 190 200
RSQPPYTDYV STRWYRAPEV LLRSSVYSSP IDVWAVGSIM AELYTFRPLF
210 220 230 240 250
PGTSEVDEIF KICQVLGTPK KSDWPEGYQL ASSMNFRFPQ CIPINLKTLI
260 270 280 290 300
PNASSEAIQL MTEMLNWDPK KRPTASQALK HPYFQVGQVL GPSAHHLDAK
310 320 330 340 350
QTLHKQLQPP EPKPSSSERD PKPLPNILDQ PAGQPQPKQG HQPLQAIQPP
360 370 380 390 400
QNTVVQPPPK QQGHHKQPQT MFPSIVKTIP TNPVSTVGHK GARRRWGQTV
410 420 430 440 450
FKSGDSCDNI EDCDLGASHS KKPSMDAFKE KKKKESPFRF PEAGLPVSNH
460 470 480 490 500
LKGENRNLHA SLKSDTNLST ASTAKQYYLK QSRYLPGVNP KNVSLVAGGK
510 520 530 540 550
DINSHSWNNQ LFPKSLGSMG ADLAFKRSNA AGNLGSYSAY SQTGCVPSFL
560 570 580 590 600
KKEVGSAGQR IHLAPLGASA ADYTWSTKTG QGQFSGRTYN PTAKNLNIVN
610 620
RTQPVPSVHG RTDWVAKYGG HR
Length:622
Mass (Da):69,897
Last modified:March 21, 2012 - v2
Checksum:i7C2056E1D116D3BF
GO
Isoform 2 (identifier: P20793-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     531-571: Missing.

Show »
Length:581
Mass (Da):65,849
Checksum:i8F4911C903FF3B3D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti581Q → R in AAA41562 (PubMed:2183027).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042475531 – 571Missing in isoform 2. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35862 mRNA. Translation: AAA41562.1.
CH473977 Genomic DNA. Translation: EDL98222.1.
BC078887 mRNA. Translation: AAH78887.1.
PIRiA34711.
RefSeqiNP_037268.1. NM_013136.1.
UniGeneiRn.9670.

Genome annotation databases

EnsembliENSRNOT00000020672; ENSRNOP00000020672; ENSRNOG00000015101. [P20793-1]
GeneIDi25677.
KEGGirno:25677.
UCSCiRGD:3036. rat. [P20793-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35862 mRNA. Translation: AAA41562.1.
CH473977 Genomic DNA. Translation: EDL98222.1.
BC078887 mRNA. Translation: AAH78887.1.
PIRiA34711.
RefSeqiNP_037268.1. NM_013136.1.
UniGeneiRn.9670.

3D structure databases

ProteinModelPortaliP20793.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020672.

PTM databases

PhosphoSitePlusiP20793.

Proteomic databases

PaxDbiP20793.
PRIDEiP20793.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020672; ENSRNOP00000020672; ENSRNOG00000015101. [P20793-1]
GeneIDi25677.
KEGGirno:25677.
UCSCiRGD:3036. rat. [P20793-1]

Organism-specific databases

CTDi4117.
RGDi3036. Mak.

Phylogenomic databases

eggNOGiKOG0661. Eukaryota.
ENOG410XPBB. LUCA.
GeneTreeiENSGT00650000093283.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiP20793.
KOiK08829.
OMAiYATYNQS.
OrthoDBiEOG091G0DU3.

Enzyme and pathway databases

BRENDAi2.7.11.22. 5301.

Miscellaneous databases

PROiP20793.

Gene expression databases

BgeeiENSRNOG00000015101.
GenevisibleiP20793. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAK_RAT
AccessioniPrimary (citable) accession number: P20793
Secondary accession number(s): G3V7Y4, Q6AYW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: March 21, 2012
Last modified: November 30, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.