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P20792 (DAF1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell surface receptor daf-1

EC=2.7.11.30
Alternative name(s):
Abnormal dauer formation protein 1
Gene names
Name:daf-1
ORF Names:F29C4.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length669 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Controls the dauer/nondauer developmental decision in a range of environmental conditions. Ref.3

Catalytic activity

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Subunit structure

May interact with daf-4 to regulate dauer larva development. Ref.3

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Head and ventral nerve cord from embryos to adults. Expressed in many sensory neurons. Subset of head neurons show coexpression with daf-4 when dauer/nondauer decision is made. Also expressed in non-neuronal cells: membraneous sheath surrounding the distal end of the intestine and in the distal tip cell of the gonad. Ref.3

Developmental stage

All stages. Ref.3

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Contains 1 GS domain.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

daf-21Q186882EBI-360236,EBI-313329

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: P20792-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: P20792-2)

The sequence of this isoform differs from the canonical sequence as follows:
     467-467: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 669650Cell surface receptor daf-1
PRO_0000024430

Regions

Topological domain20 – 170151Extracellular Potential
Transmembrane171 – 19121Helical; Potential
Topological domain192 – 669478Cytoplasmic Potential
Domain262 – 29231GS
Domain293 – 593301Protein kinase
Nucleotide binding299 – 3079ATP By similarity

Sites

Active site4231Proton acceptor By similarity
Binding site3201ATP By similarity

Amino acid modifications

Glycosylation491N-linked (GlcNAc...) Potential
Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation1331N-linked (GlcNAc...) Potential
Glycosylation1541N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence4671Missing in isoform b.
VSP_007948

Experimental info

Mutagenesis701G → R in allele p168; forms many dauer larvae. Ref.3
Mutagenesis901C → Y in allele m138; forms many dauer larvae. Ref.3
Mutagenesis1051E → A in allele m122; forms many dauer larvae; when associated with E-110. Ref.3
Mutagenesis1101G → E in allele m122; forms many dauer larvae; when associated with A-105. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 8BDD86C67C8F2A6A

FASTA66975,006
        10         20         30         40         50         60 
MRIRHVVFCL LALVYGAETS DDDLDERTNI FIRDKLIPAL KLAEVTKVNF TRLHLCHCSR 

        70         80         90        100        110        120 
EVGCNARTTG WVPGIEFLNE TDRSFYENTC YTDGSCYQSA RPSPEISHFG CMDEKSVTDE 

       130        140        150        160        170        180 
TEFHDTAAKV CTNNTKDPHA TVWICCDKGN FCANETIIHL APGPQQSSTW LILTILALLT 

       190        200        210        220        230        240 
FIVLLGIAIF LTRKSWEAKF DWYIRFKPKP GDPLRETENN VPMVTMGDGA GSSVPEVAPI 

       250        260        270        280        290        300 
EQQGSTMSTS AGNSFPPGIM PNNMKDMLDV LEETSGSGMG PTTLHKLTIG GQIRLTGRVG 

       310        320        330        340        350        360 
SGRFGNVSRG DYRGEAVAVK VFNALDEPAF HKETEIFETR MLRHPNVLRY IGSDRVDTGF 

       370        380        390        400        410        420 
VTELWLVTEY HPSGSLHDFL LENTVNIETY YNLMRSTASG LAFLHNQIGG SKESNKPAMA 

       430        440        450        460        470        480 
HRDIKSKNIM VKNDLTCAIG DLGLSLSKPE DAASDIIANE NYKCGTVRYL APEILNSTMQ 

       490        500        510        520        530        540 
FTVFESYQCA DVYSFSLVMW ETLCRCEDGD VLPREAATVI PYIEWTDRDP QDAQMFDVVC 

       550        560        570        580        590        600 
TRRLRPTENP LWKDHPEMKH IMEIIKTCWN GNPSARFTSY ICRKRMDERQ QLLLDKKAKA 

       610        620        630        640        650        660 
VAQTAGVTVQ DRKILGPQKP KDESPANGAP RIVQKEIDRE DEQENWRETA KTPNGHISSN 


DDSSRPLLG 

« Hide

Isoform b [UniParc].

Checksum: EED85A548CE780B2
Show »

FASTA66874,907

References

« Hide 'large scale' references
[1]"daf-1, a C. elegans gene controlling dauer larva development, encodes a novel receptor protein kinase."
Georgi L.L., Albert P.S., Riddle D.L.
Cell 61:635-645(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"A Caenorhabditis elegans type I TGF beta receptor can function in the absence of type II kinase to promote larval development."
Gunther C.V., Georgi L.L., Riddle D.L.
Development 127:3337-3347(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF GLY-70; CYS-90; GLU-105 AND GLY-110.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M32877 Genomic DNA. Translation: AAA28001.1.
FO080227 Genomic DNA. Translation: CCD62175.1.
FO080227 Genomic DNA. Translation: CCD62176.1.
PIRA35103.
RefSeqNP_001023159.1. NM_001027988.4. [P20792-1]
NP_001023160.1. NM_001027989.3. [P20792-2]
UniGeneCel.5220.

3D structure databases

ProteinModelPortalP20792.
SMRP20792. Positions 256-591.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid41992. 2 interactions.
IntActP20792. 1 interaction.
STRING6239.F29C4.1a.

Proteomic databases

PaxDbP20792.
PRIDEP20792.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF29C4.1a; F29C4.1a; WBGene00000897. [P20792-1]
GeneID176829.
KEGGcel:CELE_F29C4.1.
UCSCF29C4.1a. c. elegans. [P20792-1]

Organism-specific databases

CTD176829.
WormBaseF29C4.1a; CE17719; WBGene00000897; daf-1.
F29C4.1b; CE31492; WBGene00000897; daf-1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00560000076906.
HOGENOMHOG000016460.
InParanoidP20792.
OMADLTCAIG.
OrthoDBEOG7JHM5B.
PhylomeDBP20792.

Enzyme and pathway databases

BRENDA2.7.10.2. 1045.
SignaLinkP20792.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR003605. TGF_beta_rcpt_GS.
IPR000333. TGFB_receptor.
[Graphical view]
PANTHERPTHR23255. PTHR23255. 1 hit.
PfamPF00069. Pkinase. 1 hit.
PF08515. TGF_beta_GS. 1 hit.
[Graphical view]
SMARTSM00467. GS. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS51256. GS. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio894194.

Entry information

Entry nameDAF1_CAEEL
AccessionPrimary (citable) accession number: P20792
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase