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Protein

Cell surface receptor daf-1

Gene

daf-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Controls the dauer/nondauer developmental decision in a range of environmental conditions.1 Publication

Catalytic activityi

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei320 – 3201ATPPROSITE-ProRule annotation
Active sitei423 – 4231Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi299 – 3079ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. receptor signaling protein serine/threonine kinase activity Source: InterPro
  3. transforming growth factor beta-activated receptor activity Source: WormBase

GO - Biological processi

  1. dauer larval development Source: WormBase
  2. defense response to Gram-negative bacterium Source: WormBase
  3. positive regulation of transcription from RNA polymerase II promoter Source: WormBase
  4. regulation of pharyngeal pumping Source: WormBase
  5. transforming growth factor beta receptor signaling pathway Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 1045.
ReactomeiREACT_107985. Signaling by BMP.
REACT_183486. Signaling by NODAL.
SignaLinkiP20792.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell surface receptor daf-1 (EC:2.7.11.30)
Alternative name(s):
Abnormal dauer formation protein 1
Gene namesi
Name:daf-1
ORF Names:F29C4.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome IV

Organism-specific databases

WormBaseiF29C4.1a; CE17719; WBGene00000897; daf-1.
F29C4.1b; CE31492; WBGene00000897; daf-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 170151ExtracellularSequence AnalysisAdd
BLAST
Transmembranei171 – 19121HelicalSequence AnalysisAdd
BLAST
Topological domaini192 – 669478CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi70 – 701G → R in allele p168; forms many dauer larvae. 1 Publication
Mutagenesisi90 – 901C → Y in allele m138; forms many dauer larvae. 1 Publication
Mutagenesisi105 – 1051E → A in allele m122; forms many dauer larvae; when associated with E-110. 1 Publication
Mutagenesisi110 – 1101G → E in allele m122; forms many dauer larvae; when associated with A-105. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 669650Cell surface receptor daf-1PRO_0000024430Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi49 – 491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP20792.
PRIDEiP20792.

Expressioni

Tissue specificityi

Head and ventral nerve cord from embryos to adults. Expressed in many sensory neurons. Subset of head neurons show coexpression with daf-4 when dauer/nondauer decision is made. Also expressed in non-neuronal cells: membraneous sheath surrounding the distal end of the intestine and in the distal tip cell of the gonad.1 Publication

Developmental stagei

All stages.1 Publication

Interactioni

Subunit structurei

May interact with daf-4 to regulate dauer larva development.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
daf-21Q186882EBI-360236,EBI-313329

Protein-protein interaction databases

BioGridi41992. 2 interactions.
IntActiP20792. 1 interaction.
STRINGi6239.F29C4.1a.

Structurei

3D structure databases

ProteinModelPortaliP20792.
SMRiP20792. Positions 262-591.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini262 – 29231GSPROSITE-ProRule annotationAdd
BLAST
Domaini293 – 593301Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GS domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118876.
HOGENOMiHOG000016460.
InParanoidiP20792.
OMAiDLTCAIG.
OrthoDBiEOG7JHM5B.
PhylomeDBiP20792.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR003605. TGF_beta_rcpt_GS.
IPR000333. TGFB_receptor.
[Graphical view]
PANTHERiPTHR23255. PTHR23255. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
PF08515. TGF_beta_GS. 1 hit.
[Graphical view]
SMARTiSM00467. GS. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51256. GS. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: P20792-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRIRHVVFCL LALVYGAETS DDDLDERTNI FIRDKLIPAL KLAEVTKVNF
60 70 80 90 100
TRLHLCHCSR EVGCNARTTG WVPGIEFLNE TDRSFYENTC YTDGSCYQSA
110 120 130 140 150
RPSPEISHFG CMDEKSVTDE TEFHDTAAKV CTNNTKDPHA TVWICCDKGN
160 170 180 190 200
FCANETIIHL APGPQQSSTW LILTILALLT FIVLLGIAIF LTRKSWEAKF
210 220 230 240 250
DWYIRFKPKP GDPLRETENN VPMVTMGDGA GSSVPEVAPI EQQGSTMSTS
260 270 280 290 300
AGNSFPPGIM PNNMKDMLDV LEETSGSGMG PTTLHKLTIG GQIRLTGRVG
310 320 330 340 350
SGRFGNVSRG DYRGEAVAVK VFNALDEPAF HKETEIFETR MLRHPNVLRY
360 370 380 390 400
IGSDRVDTGF VTELWLVTEY HPSGSLHDFL LENTVNIETY YNLMRSTASG
410 420 430 440 450
LAFLHNQIGG SKESNKPAMA HRDIKSKNIM VKNDLTCAIG DLGLSLSKPE
460 470 480 490 500
DAASDIIANE NYKCGTVRYL APEILNSTMQ FTVFESYQCA DVYSFSLVMW
510 520 530 540 550
ETLCRCEDGD VLPREAATVI PYIEWTDRDP QDAQMFDVVC TRRLRPTENP
560 570 580 590 600
LWKDHPEMKH IMEIIKTCWN GNPSARFTSY ICRKRMDERQ QLLLDKKAKA
610 620 630 640 650
VAQTAGVTVQ DRKILGPQKP KDESPANGAP RIVQKEIDRE DEQENWRETA
660
KTPNGHISSN DDSSRPLLG
Length:669
Mass (Da):75,006
Last modified:February 1, 1991 - v1
Checksum:i8BDD86C67C8F2A6A
GO
Isoform b (identifier: P20792-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     467-467: Missing.

Note: No experimental confirmation available.

Show »
Length:668
Mass (Da):74,907
Checksum:iEED85A548CE780B2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei467 – 4671Missing in isoform b. CuratedVSP_007948

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32877 Genomic DNA. Translation: AAA28001.1.
FO080227 Genomic DNA. Translation: CCD62175.1.
FO080227 Genomic DNA. Translation: CCD62176.1.
PIRiA35103.
RefSeqiNP_001023159.1. NM_001027988.4. [P20792-1]
NP_001023160.1. NM_001027989.3. [P20792-2]
UniGeneiCel.5220.

Genome annotation databases

EnsemblMetazoaiF29C4.1a; F29C4.1a; WBGene00000897. [P20792-1]
GeneIDi176829.
KEGGicel:CELE_F29C4.1.
UCSCiF29C4.1a. c. elegans. [P20792-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32877 Genomic DNA. Translation: AAA28001.1.
FO080227 Genomic DNA. Translation: CCD62175.1.
FO080227 Genomic DNA. Translation: CCD62176.1.
PIRiA35103.
RefSeqiNP_001023159.1. NM_001027988.4. [P20792-1]
NP_001023160.1. NM_001027989.3. [P20792-2]
UniGeneiCel.5220.

3D structure databases

ProteinModelPortaliP20792.
SMRiP20792. Positions 262-591.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41992. 2 interactions.
IntActiP20792. 1 interaction.
STRINGi6239.F29C4.1a.

Proteomic databases

PaxDbiP20792.
PRIDEiP20792.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF29C4.1a; F29C4.1a; WBGene00000897. [P20792-1]
GeneIDi176829.
KEGGicel:CELE_F29C4.1.
UCSCiF29C4.1a. c. elegans. [P20792-1]

Organism-specific databases

CTDi176829.
WormBaseiF29C4.1a; CE17719; WBGene00000897; daf-1.
F29C4.1b; CE31492; WBGene00000897; daf-1.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118876.
HOGENOMiHOG000016460.
InParanoidiP20792.
OMAiDLTCAIG.
OrthoDBiEOG7JHM5B.
PhylomeDBiP20792.

Enzyme and pathway databases

BRENDAi2.7.10.2. 1045.
ReactomeiREACT_107985. Signaling by BMP.
REACT_183486. Signaling by NODAL.
SignaLinkiP20792.

Miscellaneous databases

NextBioi894194.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR003605. TGF_beta_rcpt_GS.
IPR000333. TGFB_receptor.
[Graphical view]
PANTHERiPTHR23255. PTHR23255. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
PF08515. TGF_beta_GS. 1 hit.
[Graphical view]
SMARTiSM00467. GS. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51256. GS. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "daf-1, a C. elegans gene controlling dauer larva development, encodes a novel receptor protein kinase."
    Georgi L.L., Albert P.S., Riddle D.L.
    Cell 61:635-645(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "A Caenorhabditis elegans type I TGF beta receptor can function in the absence of type II kinase to promote larval development."
    Gunther C.V., Georgi L.L., Riddle D.L.
    Development 127:3337-3347(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF GLY-70; CYS-90; GLU-105 AND GLY-110.

Entry informationi

Entry nameiDAF1_CAEEL
AccessioniPrimary (citable) accession number: P20792
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: January 7, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.