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Protein

Neurotensin receptor type 1

Gene

Ntsr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for the tridecapeptide neurotensin (NTS) (PubMed:23051748, PubMed:24453215, PubMed:26205105). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis (By similarity).By similarity4 Publications

GO - Molecular functioni

  • G-protein coupled neurotensin receptor activity Source: UniProtKB
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD
  • protein N-terminus binding Source: RGD

GO - Biological processi

  • adult locomotory behavior Source: Ensembl
  • D-aspartate import Source: RGD
  • detection of temperature stimulus involved in sensory perception of pain Source: RGD
  • inositol phosphate catabolic process Source: RGD
  • learning Source: RGD
  • L-glutamate import into cell Source: RGD
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of release of sequestered calcium ion into cytosol Source: RGD
  • negative regulation of systemic arterial blood pressure Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of arachidonic acid secretion Source: RGD
  • positive regulation of cation channel activity Source: RGD
  • positive regulation of gamma-aminobutyric acid secretion Source: RGD
  • positive regulation of glutamate secretion Source: RGD
  • positive regulation of inhibitory postsynaptic potential Source: RGD
  • positive regulation of inositol phosphate biosynthetic process Source: RGD
  • positive regulation of release of sequestered calcium ion into cytosol Source: RGD
  • regulation of action potential Source: RGD
  • regulation of membrane depolarization Source: RGD
  • regulation of respiratory gaseous exchange Source: RGD
  • regulation of sensory perception of pain Source: RGD
  • response to lipid Source: RGD
  • temperature homeostasis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurotensin receptor type 1
Short name:
NT-R-1
Short name:
NTR1
Alternative name(s):
High-affinity levocabastine-insensitive neurotensin receptor
NTRH
Gene namesi
Name:Ntsr1
Synonyms:Ntsr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1306076. Ntsr1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 68Extracellular1 PublicationAdd BLAST68
Transmembranei69 – 89Helical; Name=1Add BLAST21
Topological domaini90 – 103Cytoplasmic1 PublicationAdd BLAST14
Transmembranei104 – 123Helical; Name=2Add BLAST20
Topological domaini124 – 143Extracellular1 PublicationAdd BLAST20
Transmembranei144 – 165Helical; Name=3Add BLAST22
Topological domaini166 – 185Cytoplasmic1 PublicationAdd BLAST20
Transmembranei186 – 206Helical; Name=4Add BLAST21
Topological domaini207 – 235Extracellular1 PublicationAdd BLAST29
Transmembranei236 – 260Helical; Name=5Add BLAST25
Topological domaini261 – 308Cytoplasmic1 PublicationAdd BLAST48
Transmembranei309 – 330Helical; Name=6Add BLAST22
Topological domaini331 – 348Extracellular1 PublicationAdd BLAST18
Transmembranei349 – 369Helical; Name=7Add BLAST21
Topological domaini370 – 424Cytoplasmic1 PublicationAdd BLAST55

GO - Cellular componenti

  • axon Source: RGD
  • axon terminus Source: RGD
  • cell surface Source: RGD
  • cytoplasm Source: RGD
  • cytoplasmic side of plasma membrane Source: RGD
  • dendrite Source: RGD
  • dendritic shaft Source: RGD
  • dendritic spine Source: RGD
  • endoplasmic reticulum Source: RGD
  • Golgi apparatus Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • membrane raft Source: UniProtKB
  • mitochondrion Source: RGD
  • neuronal cell body Source: RGD
  • neuron spine Source: RGD
  • perikaryon Source: RGD
  • plasma membrane Source: RGD
  • symmetric synapse Source: RGD
  • synapse Source: RGD
  • terminal bouton Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi166E → A: Abolishes signaling via G-proteins; when associated with A-310 and A-358. 1 Publication1
Mutagenesisi310L → A: Abolishes signaling via G-proteins; when associated with A-166 and A-358. 1 Publication1
Mutagenesisi358F → A: Abolishes signaling via G-proteins; when associated with A-166 and A-310. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3027.
GuidetoPHARMACOLOGYi309.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699481 – 424Neurotensin receptor type 1Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi4N-linked (GlcNAc...)Sequence analysis1
Glycosylationi38N-linked (GlcNAc...)Sequence analysis1
Glycosylationi42N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi142 ↔ 225PROSITE-ProRule annotation2 Publications
Lipidationi386S-palmitoyl cysteineBy similarity1
Lipidationi388S-palmitoyl cysteineBy similarity1

Post-translational modificationi

N-gycosylated.By similarity
Palmitoylated; this is required for normal localization at membrane rafts and normal GNA11-mediated activation of down-stream signaling cascades. The palmitoylation level increases in response to neurotensin treatment.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP20789.

PTM databases

iPTMnetiP20789.
PhosphoSitePlusiP20789.
SwissPalmiP20789.

Expressioni

Tissue specificityi

Detected in brain and small intestine.1 Publication

Gene expression databases

BgeeiENSRNOG00000028708.
GenevisibleiP20789. RN.

Interactioni

Subunit structurei

Interacts (palmitoylated form) with GNA11.By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD
  • protein N-terminus binding Source: RGD

Protein-protein interaction databases

DIPiDIP-59956N.
STRINGi10116.ENSRNOP00000035997.

Chemistry databases

BindingDBiP20789.

Structurei

Secondary structure

1424
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni51 – 55Combined sources5
Helixi61 – 92Combined sources32
Helixi97 – 119Combined sources23
Helixi121 – 127Combined sources7
Turni128 – 130Combined sources3
Helixi138 – 172Combined sources35
Helixi174 – 180Combined sources7
Helixi183 – 200Combined sources18
Helixi203 – 207Combined sources5
Beta strandi208 – 212Combined sources5
Beta strandi215 – 217Combined sources3
Helixi220 – 222Combined sources3
Beta strandi223 – 227Combined sources5
Helixi231 – 245Combined sources15
Helixi247 – 267Combined sources21
Helixi298 – 300Combined sources3
Helixi301 – 333Combined sources33
Helixi336 – 338Combined sources3
Helixi341 – 368Combined sources28
Beta strandi371 – 373Combined sources3
Helixi376 – 381Combined sources6
Beta strandi413 – 418Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZEVX-ray3.00A/B50-390[»]
4BUOX-ray2.75A/B50-390[»]
4BV0X-ray3.10A/B50-390[»]
4BWBX-ray3.57A/B50-390[»]
4GRVX-ray2.80A43-268[»]
A300-396[»]
4XEEX-ray2.90A43-396[»]
4XESX-ray2.60A43-396[»]
ProteinModelPortaliP20789.
SMRiP20789.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni326 – 349Neurotensin bindingAdd BLAST24

Domaini

The ligand binding pocket consists mainly of extracellular loops ECL2 and ECL3, as well as transmembrane regions TM6 and TM7.2 Publications

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR1 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00700000104070.
HOGENOMiHOG000231271.
HOVERGENiHBG098285.
InParanoidiP20789.
KOiK04211.
OMAiTVMVRQA.
PhylomeDBiP20789.
TreeFamiTF337167.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003985. NT1_rcpt.
IPR003984. NT_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01479. NEUROTENSINR.
PR01480. NEUROTENSN1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLNSSVPQG TPGEPDAQPF SGPQSEMEAT FLALSLSNGS GNTSESDTAG
60 70 80 90 100
PNSDLDVNTD IYSKVLVTAI YLALFVVGTV GNSVTAFTLA RKKSLQSLQS
110 120 130 140 150
TVHYHLGSLA LSDLLILLLA MPVELYNFIW VHHPWAFGDA GCRGYYFLRD
160 170 180 190 200
ACTYATALNV ASLSVERYLA ICHPFKAKTL MSRSRTKKFI SAIWLASALL
210 220 230 240 250
AIPMLFTMGL QNRSGDGTHP GGLVCTPIVD TATVKVVIQV NTFMSFLFPM
260 270 280 290 300
LVISILNTVI ANKLTVMVHQ AAEQGRVCTV GTHNGLEHST FNMTIEPGRV
310 320 330 340 350
QALRHGVLVL RAVVIAFVVC WLPYHVRRLM FCYISDEQWT TFLFDFYHYF
360 370 380 390 400
YMLTNALFYV SSAINPILYN LVSANFRQVF LSTLACLCPG WRHRRKKRPT
410 420
FSRKPNSMSS NHAFSTSATR ETLY
Length:424
Mass (Da):47,055
Last modified:February 1, 1991 - v1
Checksum:iA9C2F7EAF8D9BCD3
GO

Sequence databases

PIRiJH0164.
RefSeqiNP_001102437.1. NM_001108967.1.
XP_006235850.1. XM_006235788.1.
UniGeneiRn.200149.

Genome annotation databases

EnsembliENSRNOT00000039780; ENSRNOP00000035997; ENSRNOG00000028708.
ENSRNOT00000082073; ENSRNOP00000072865; ENSRNOG00000028708.
GeneIDi366274.
KEGGirno:366274.
UCSCiRGD:1306076. rat.

Cross-referencesi

Sequence databases

PIRiJH0164.
RefSeqiNP_001102437.1. NM_001108967.1.
XP_006235850.1. XM_006235788.1.
UniGeneiRn.200149.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZEVX-ray3.00A/B50-390[»]
4BUOX-ray2.75A/B50-390[»]
4BV0X-ray3.10A/B50-390[»]
4BWBX-ray3.57A/B50-390[»]
4GRVX-ray2.80A43-268[»]
A300-396[»]
4XEEX-ray2.90A43-396[»]
4XESX-ray2.60A43-396[»]
ProteinModelPortaliP20789.
SMRiP20789.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59956N.
STRINGi10116.ENSRNOP00000035997.

Chemistry databases

BindingDBiP20789.
ChEMBLiCHEMBL3027.
GuidetoPHARMACOLOGYi309.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP20789.
PhosphoSitePlusiP20789.
SwissPalmiP20789.

Proteomic databases

PaxDbiP20789.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000039780; ENSRNOP00000035997; ENSRNOG00000028708.
ENSRNOT00000082073; ENSRNOP00000072865; ENSRNOG00000028708.
GeneIDi366274.
KEGGirno:366274.
UCSCiRGD:1306076. rat.

Organism-specific databases

CTDi4923.
RGDi1306076. Ntsr1.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00700000104070.
HOGENOMiHOG000231271.
HOVERGENiHBG098285.
InParanoidiP20789.
KOiK04211.
OMAiTVMVRQA.
PhylomeDBiP20789.
TreeFamiTF337167.

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-416476. G alpha (q) signalling events.

Miscellaneous databases

PROiP20789.

Gene expression databases

BgeeiENSRNOG00000028708.
GenevisibleiP20789. RN.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003985. NT1_rcpt.
IPR003984. NT_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01479. NEUROTENSINR.
PR01480. NEUROTENSN1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTR1_RAT
AccessioniPrimary (citable) accession number: P20789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.