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P20789

- NTR1_RAT

UniProt

P20789 - NTR1_RAT

Protein

Neurotensin receptor type 1

Gene

Ntsr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for the tridecapeptide neurotensin (NTS). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis.1 Publication

    GO - Molecular functioni

    1. G-protein coupled neurotensin receptor activity Source: UniProtKB

    GO - Biological processi

    1. adult locomotory behavior Source: Ensembl
    2. negative regulation of apoptotic process Source: UniProtKB
    3. neuropeptide signaling pathway Source: GOC

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neurotensin receptor type 1
    Short name:
    NT-R-1
    Short name:
    NTR1
    Alternative name(s):
    High-affinity levocabastine-insensitive neurotensin receptor
    NTRH
    Gene namesi
    Name:Ntsr1
    Synonyms:Ntsr
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi1306076. Ntsr1.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Membrane raft By similarity
    Note: Palmitoylation is required for localization at CAV1-enriched membrane rafts.By similarity

    GO - Cellular componenti

    1. integral component of plasma membrane Source: UniProtKB
    2. membrane raft Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 424424Neurotensin receptor type 1PRO_0000069948Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi4 – 41N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi42 – 421N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi142 ↔ 2252 PublicationsPROSITE-ProRule annotation
    Lipidationi386 – 3861S-palmitoyl cysteineBy similarity
    Lipidationi388 – 3881S-palmitoyl cysteineBy similarity

    Post-translational modificationi

    N-gycosylated.By similarity
    Palmitoylated; this is required for normal localization at membrane rafts and normal GNA11-mediated activation of down-stream signaling cascades. The palmitoylation level increases in response to neurotensin treatment By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    PTM databases

    PhosphoSiteiP20789.

    Expressioni

    Gene expression databases

    GenevestigatoriP20789.

    Interactioni

    Subunit structurei

    Interacts (palmitoylated form) with GNA11.By similarity

    Protein-protein interaction databases

    DIPiDIP-59956N.
    STRINGi10116.ENSRNOP00000035997.

    Structurei

    Secondary structure

    1
    424
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi53 – 553
    Helixi61 – 9030
    Helixi99 – 11820
    Helixi120 – 12910
    Helixi138 – 17235
    Helixi174 – 1785
    Helixi183 – 20119
    Helixi204 – 2074
    Beta strandi208 – 2125
    Beta strandi214 – 2174
    Helixi220 – 2223
    Beta strandi223 – 2275
    Helixi231 – 24515
    Helixi247 – 26923
    Helixi298 – 33336
    Turni336 – 3383
    Helixi341 – 37232
    Helixi374 – 38310

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ZEVX-ray3.00A/B50-390[»]
    4BUOX-ray2.75A/B50-390[»]
    4BV0X-ray3.10A/B50-390[»]
    4BWBX-ray3.57A/B50-390[»]
    4GRVX-ray2.80A43-268[»]
    A300-396[»]
    ProteinModelPortaliP20789.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6868Extracellular1 PublicationAdd
    BLAST
    Topological domaini90 – 10314Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini124 – 14320Extracellular1 PublicationAdd
    BLAST
    Topological domaini166 – 18823Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini211 – 23525Extracellular1 PublicationAdd
    BLAST
    Topological domaini261 – 30848Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini331 – 34818Extracellular1 PublicationAdd
    BLAST
    Topological domaini370 – 42455Cytoplasmic1 PublicationAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei69 – 8921Helical; Name=1Add
    BLAST
    Transmembranei104 – 12320Helical; Name=2Add
    BLAST
    Transmembranei144 – 16522Helical; Name=3Add
    BLAST
    Transmembranei189 – 21022Helical; Name=4Add
    BLAST
    Transmembranei236 – 26025Helical; Name=5Add
    BLAST
    Transmembranei309 – 33022Helical; Name=6Add
    BLAST
    Transmembranei349 – 36921Helical; Name=7Add
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni326 – 34924Neurotensin bindingAdd
    BLAST

    Domaini

    The ligand binding pocket consists mainly of extracellular loops ECL2 and ECL3, as well as transmembranes TM6 and TM7.

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR1 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG303702.
    GeneTreeiENSGT00700000104470.
    HOGENOMiHOG000231271.
    HOVERGENiHBG098285.
    InParanoidiP20789.
    KOiK04211.
    OMAiTVMVRQA.
    OrthoDBiEOG75F4DR.
    PhylomeDBiP20789.
    TreeFamiTF337167.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR003985. NT1_rcpt.
    IPR003984. NT_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR01479. NEUROTENSINR.
    PR01480. NEUROTENSN1R.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20789-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHLNSSVPQG TPGEPDAQPF SGPQSEMEAT FLALSLSNGS GNTSESDTAG    50
    PNSDLDVNTD IYSKVLVTAI YLALFVVGTV GNSVTAFTLA RKKSLQSLQS 100
    TVHYHLGSLA LSDLLILLLA MPVELYNFIW VHHPWAFGDA GCRGYYFLRD 150
    ACTYATALNV ASLSVERYLA ICHPFKAKTL MSRSRTKKFI SAIWLASALL 200
    AIPMLFTMGL QNRSGDGTHP GGLVCTPIVD TATVKVVIQV NTFMSFLFPM 250
    LVISILNTVI ANKLTVMVHQ AAEQGRVCTV GTHNGLEHST FNMTIEPGRV 300
    QALRHGVLVL RAVVIAFVVC WLPYHVRRLM FCYISDEQWT TFLFDFYHYF 350
    YMLTNALFYV SSAINPILYN LVSANFRQVF LSTLACLCPG WRHRRKKRPT 400
    FSRKPNSMSS NHAFSTSATR ETLY 424
    Length:424
    Mass (Da):47,055
    Last modified:February 1, 1991 - v1
    Checksum:iA9C2F7EAF8D9BCD3
    GO

    Sequence databases

    PIRiJH0164.
    RefSeqiNP_001102437.1. NM_001108967.1.
    XP_006235850.1. XM_006235788.1.
    UniGeneiRn.200149.

    Genome annotation databases

    EnsembliENSRNOT00000039780; ENSRNOP00000035997; ENSRNOG00000028708.
    GeneIDi366274.
    KEGGirno:366274.
    UCSCiRGD:1306076. rat.

    Cross-referencesi

    Sequence databases

    PIRi JH0164.
    RefSeqi NP_001102437.1. NM_001108967.1.
    XP_006235850.1. XM_006235788.1.
    UniGenei Rn.200149.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ZEV X-ray 3.00 A/B 50-390 [» ]
    4BUO X-ray 2.75 A/B 50-390 [» ]
    4BV0 X-ray 3.10 A/B 50-390 [» ]
    4BWB X-ray 3.57 A/B 50-390 [» ]
    4GRV X-ray 2.80 A 43-268 [» ]
    A 300-396 [» ]
    ProteinModelPortali P20789.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59956N.
    STRINGi 10116.ENSRNOP00000035997.

    Chemistry

    BindingDBi P20789.
    ChEMBLi CHEMBL3027.
    GuidetoPHARMACOLOGYi 309.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P20789.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000039780 ; ENSRNOP00000035997 ; ENSRNOG00000028708 .
    GeneIDi 366274.
    KEGGi rno:366274.
    UCSCi RGD:1306076. rat.

    Organism-specific databases

    CTDi 4923.
    RGDi 1306076. Ntsr1.

    Phylogenomic databases

    eggNOGi NOG303702.
    GeneTreei ENSGT00700000104470.
    HOGENOMi HOG000231271.
    HOVERGENi HBG098285.
    InParanoidi P20789.
    KOi K04211.
    OMAi TVMVRQA.
    OrthoDBi EOG75F4DR.
    PhylomeDBi P20789.
    TreeFami TF337167.

    Miscellaneous databases

    NextBioi 689103.
    PROi P20789.

    Gene expression databases

    Genevestigatori P20789.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR003985. NT1_rcpt.
    IPR003984. NT_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR01479. NEUROTENSINR.
    PR01480. NEUROTENSN1R.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and functional expression of the cloned rat neurotensin receptor."
      Tanaka K., Masu M., Nakanishi S.
      Neuron 4:847-854(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: Brain.
    2. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 43-396 IN COMPLEX WITH NEUROTENSIN FRAGMENT, LIGAND BINDING DOMAIN, DISULFIDE BOND.
    3. "Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli."
      Egloff P., Hillenbrand M., Klenk C., Batyuk A., Heine P., Balada S., Schlinkmann K.M., Scott D.J., Schutz M., Pluckthun A.
      Proc. Natl. Acad. Sci. U.S.A. 111:E655-E662(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF 50-390 IN COMPLEX WITH NEUROTENSIN FRAGMENT, DISULFIDE BOND, SUBCELLULAR LOCATION, TOPOLOGY, FUNCTION.

    Entry informationi

    Entry nameiNTR1_RAT
    AccessioniPrimary (citable) accession number: P20789
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3