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Protein

Cytochrome b-c1 complex subunit Rieske, mitochondrial

Gene

Uqcrfs1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.By similarity

Catalytic activityi

Quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+.

Cofactori

[2Fe-2S] clusterPROSITE-ProRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi217 – 2171Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi219 – 2191Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
Metal bindingi236 – 2361Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi239 – 2391Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein complex binding Source: RGD
  • ubiquinol-cytochrome-c reductase activity Source: RGD

GO - Biological processi

  • response to antibiotic Source: RGD
  • response to drug Source: RGD
  • response to hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-611105. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:1.10.2.2)
Alternative name(s):
Complex III subunit 5
Cytochrome b-c1 complex subunit 5
Liver regeneration-related protein LRRGT00195
Rieske iron-sulfur protein
Short name:
RISP
Ubiquinol-cytochrome c reductase iron-sulfur subunit
Cleaved into the following chain:
Alternative name(s):
Complex III subunit IX
Ubiquinol-cytochrome c reductase 8 kDa protein
Gene namesi
Name:Uqcrfs1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi628838. Uqcrfs1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei103 – 14038HelicalBy similarityAdd
BLAST

GO - Cellular componenti

  • mitochondrial membrane Source: RGD
  • mitochondrial respiratory chain complex III Source: RGD
  • myelin sheath Source: Ensembl
  • respiratory chain complex III Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7878MitochondrionBy similarityAdd
BLAST
Chaini1 – 7878Cytochrome b-c1 complex subunit 11By similarityPRO_0000307248Add
BLAST
Chaini79 – 274196Cytochrome b-c1 complex subunit Rieske, mitochondrialPRO_0000030671Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi222 ↔ 238PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP20788.
PRIDEiP20788.

PTM databases

iPTMnetiP20788.
PhosphoSiteiP20788.
SwissPalmiP20788.

Expressioni

Gene expression databases

BgeeiENSRNOG00000018281.
GenevisibleiP20788. RN.

Interactioni

Subunit structurei

The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

IntActiP20788. 1 interaction.
STRINGi10116.ENSRNOP00000024609.

Structurei

3D structure databases

ProteinModelPortaliP20788.
SMRiP20788. Positions 1-57, 79-274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini187 – 27286RieskePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1671. Eukaryota.
COG0723. LUCA.
GeneTreeiENSGT00390000001014.
HOGENOMiHOG000255193.
HOVERGENiHBG001040.
InParanoidiP20788.
KOiK00411.
OMAiPFYEFPD.
OrthoDBiEOG091G0MOP.
PhylomeDBiP20788.
TreeFamiTF105037.

Family and domain databases

Gene3Di1.20.5.270. 1 hit.
2.102.10.10. 1 hit.
InterProiIPR011070. Globular_prot_asu/bsu.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
IPR015248. Ubiqinol_cyt_c_Rdtase_N.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
IPR004192. Ubiquinol_cyt_Rdtase_TM.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF00355. Rieske. 1 hit.
PF09165. Ubiq-Cytc-red_N. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF56568. SSF56568. 1 hit.
TIGRFAMsiTIGR01416. Rieske_proteo. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSVAARSGP FAPVLSATSR GVAGALRPLL QSAVPATSEP PVLDVKRPFL
60 70 80 90 100
CRESLSGQAA TRPLVATVGL NVPASVRYSH TDIKVPDFSD YRRAEVLDST
110 120 130 140 150
KSSKESSEAR KGFSYLVTAT TTVGVAYAAK NAVSQFVSSM SASADVLAMS
160 170 180 190 200
KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT KKEIDQEAAV EVSQLRDPQH
210 220 230 240 250
DLERVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG SHYDASGRIR
260 270
KGPAPLNLEV PTYEFTSGDV VVVG
Length:274
Mass (Da):29,446
Last modified:September 13, 2004 - v2
Checksum:i6A0E0A07E956340B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY539946 mRNA. Translation: AAS66286.1.
BC085339 mRNA. Translation: AAH85339.1.
M24542 Genomic DNA. Translation: AAA42051.1.
PIRiA32296.
RefSeqiNP_001008888.1. NM_001008888.1.
UniGeneiRn.2603.

Genome annotation databases

EnsembliENSRNOT00000024609; ENSRNOP00000024609; ENSRNOG00000018281.
GeneIDi291103.
KEGGirno:291103.
UCSCiRGD:628838. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY539946 mRNA. Translation: AAS66286.1.
BC085339 mRNA. Translation: AAH85339.1.
M24542 Genomic DNA. Translation: AAA42051.1.
PIRiA32296.
RefSeqiNP_001008888.1. NM_001008888.1.
UniGeneiRn.2603.

3D structure databases

ProteinModelPortaliP20788.
SMRiP20788. Positions 1-57, 79-274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20788. 1 interaction.
STRINGi10116.ENSRNOP00000024609.

PTM databases

iPTMnetiP20788.
PhosphoSiteiP20788.
SwissPalmiP20788.

Proteomic databases

PaxDbiP20788.
PRIDEiP20788.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024609; ENSRNOP00000024609; ENSRNOG00000018281.
GeneIDi291103.
KEGGirno:291103.
UCSCiRGD:628838. rat.

Organism-specific databases

CTDi7386.
RGDi628838. Uqcrfs1.

Phylogenomic databases

eggNOGiKOG1671. Eukaryota.
COG0723. LUCA.
GeneTreeiENSGT00390000001014.
HOGENOMiHOG000255193.
HOVERGENiHBG001040.
InParanoidiP20788.
KOiK00411.
OMAiPFYEFPD.
OrthoDBiEOG091G0MOP.
PhylomeDBiP20788.
TreeFamiTF105037.

Enzyme and pathway databases

ReactomeiR-RNO-611105. Respiratory electron transport.

Miscellaneous databases

PROiP20788.

Gene expression databases

BgeeiENSRNOG00000018281.
GenevisibleiP20788. RN.

Family and domain databases

Gene3Di1.20.5.270. 1 hit.
2.102.10.10. 1 hit.
InterProiIPR011070. Globular_prot_asu/bsu.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
IPR015248. Ubiqinol_cyt_c_Rdtase_N.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
IPR004192. Ubiquinol_cyt_Rdtase_TM.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF00355. Rieske. 1 hit.
PF09165. Ubiq-Cytc-red_N. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF56568. SSF56568. 1 hit.
TIGRFAMsiTIGR01416. Rieske_proteo. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUCRI_RAT
AccessioniPrimary (citable) accession number: P20788
Secondary accession number(s): Q6QI13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: September 13, 2004
Last modified: September 7, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The Rieske protein is a high potential 2Fe-2S protein.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.