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Protein

Cytochrome b-c1 complex subunit Rieske, mitochondrial

Gene

Uqcrfs1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochrome b-c1 complex subunit Rieske, mitochondrial: Component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis (By similarity). Incorporation of UQCRFS1 is the penultimate step in complex III assembly (By similarity).By similarity
Cytochrome b-c1 complex subunit 9: Possible component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis (By similarity). UQCRFS1 undergoes proteolytic processing once it is incorporated in the complex III dimer, including this fragment, called subunit 9, which corresponds to the transit peptide (By similarity). The proteolytic processing is necessary for the correct insertion of UQCRFS1 in the complex III dimer, but the persistence of UQCRFS1-derived fragments may prevent newly imported UQCRFS1 to be processed and assembled into complex III and is detrimental for the complex III structure and function (By similarity). It is therefore unsure whether the UQCRFS1 fragments, including this fragment, are structural subunits (By similarity).By similarity

Miscellaneous

The Rieske protein is a high potential 2Fe-2S protein.

Caution

Several peptides are generated during UQCRFS1 insertion. According to some authors, the identification of the transit peptide as the subunit 9, does not necessary imply that it must be considered as a structural subunit of the complex III dimer as additional fragments from UQCRFS1 are also present.By similarity

Catalytic activityi

Quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+.

Cofactori

[2Fe-2S] clusterPROSITE-ProRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit. Fe-S cluster delivery to the Rieske protein is mediated by components of the iron sulfur (Fe-S) cluster assembly machinery that reside in the mitochondrial matrix (including HSC20 and LYRM7) (By similarity).PROSITE-ProRule annotationBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi217Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi219Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi236Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi239Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • ubiquinol-cytochrome-c reductase activity Source: RGD

GO - Biological processi

  • response to antibiotic Source: RGD
  • response to drug Source: RGD
  • response to hormone Source: RGD

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-611105 Respiratory electron transport

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:1.10.2.2)
Alternative name(s):
Complex III subunit 5
Cytochrome b-c1 complex subunit 5
Liver regeneration-related protein LRRGT00195
Rieske iron-sulfur protein
Short name:
RISP
Rieske protein UQCRFS1Curated
Ubiquinol-cytochrome c reductase iron-sulfur subunit
Cleaved into the following chain:
Cytochrome b-c1 complex subunit 9By similarity
Short name:
Su9By similarity
Short name:
Subunit 9By similarity
Alternative name(s):
8 kDa subunit 9By similarity
Complex III subunit IX
Cytochrome b-c1 complex subunit 11
Ubiquinol-cytochrome c reductase 8 kDa proteinBy similarity
Gene namesi
Name:Uqcrfs1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi628838 Uqcrfs1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei103 – 140HelicalBy similarityAdd BLAST38

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 78MitochondrionBy similarityAdd BLAST78
ChainiPRO_00003072481 – 78Cytochrome b-c1 complex subunit 9By similarityAdd BLAST78
ChainiPRO_000003067179 – 274Cytochrome b-c1 complex subunit Rieske, mitochondrialAdd BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi222 ↔ 238PROSITE-ProRule annotation

Post-translational modificationi

Proteolytic processing is necessary for the correct insertion of UQCRFS1 in the complex III dimer (By similarity). Several fragments are generated during UQCRFS1 insertion, most probably due to the endogenous matrix-processing peptidase (MPP) activity (By similarity). The action of the protease is also necessary for the clearance of the UQCRFS1 fragments (By similarity).By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP20788
PRIDEiP20788

PTM databases

iPTMnetiP20788
PhosphoSitePlusiP20788
SwissPalmiP20788

Expressioni

Gene expression databases

BgeeiENSRNOG00000018281
GenevisibleiP20788 RN

Interactioni

Subunit structurei

Binds to the mitochondrial respiratory complex III dimer. The monomeric module of the mitochondrial respiratory complex III contains 11 subunits: 3 respiratory subunits involved in its catalytic activity (cytochrome b, cytochrome c1 and Rieske protein UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1) (By similarity). Incorporation of the Rieske protein UQCRFS1 is the penultimate step in complex III assembly (By similarity). Interacts with TTC19, which is involved in the clearance of UQCRFS1 fragments (By similarity).By similarity

Protein-protein interaction databases

BioGridi253421, 1 interactor
CORUMiP20788
IntActiP20788, 1 interactor
STRINGi10116.ENSRNOP00000024609

Structurei

3D structure databases

ProteinModelPortaliP20788
SMRiP20788
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 272RieskePROSITE-ProRule annotationAdd BLAST86

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1671 Eukaryota
COG0723 LUCA
GeneTreeiENSGT00390000001014
HOGENOMiHOG000255193
HOVERGENiHBG001040
InParanoidiP20788
KOiK00411
OMAiERKAFSY
OrthoDBiEOG091G0MOP
PhylomeDBiP20788
TreeFamiTF105037

Family and domain databases

Gene3Di1.20.5.270, 1 hit
2.102.10.10, 1 hit
InterProiView protein in InterPro
IPR037008 bc1_Rieske_TM_sf
IPR011070 Globular_prot_asu/bsu
IPR017941 Rieske_2Fe-2S
IPR036922 Rieske_2Fe-2S_sf
IPR014349 Rieske_Fe-S_prot
IPR005805 Rieske_Fe-S_prot_C
IPR004192 Rieske_TM
IPR015248 Ubiqinol_cyt_c_Rdtase_N
IPR006317 Ubiquinol_cyt_c_Rdtase_Fe-S-su
PANTHERiPTHR10134 PTHR10134, 1 hit
PfamiView protein in Pfam
PF00355 Rieske, 1 hit
PF09165 Ubiq-Cytc-red_N, 1 hit
PF02921 UCR_TM, 1 hit
PRINTSiPR00162 RIESKE
SUPFAMiSSF50022 SSF50022, 1 hit
SSF56568 SSF56568, 1 hit
TIGRFAMsiTIGR01416 Rieske_proteo, 1 hit
PROSITEiView protein in PROSITE
PS51296 RIESKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSVAARSGP FAPVLSATSR GVAGALRPLL QSAVPATSEP PVLDVKRPFL
60 70 80 90 100
CRESLSGQAA TRPLVATVGL NVPASVRYSH TDIKVPDFSD YRRAEVLDST
110 120 130 140 150
KSSKESSEAR KGFSYLVTAT TTVGVAYAAK NAVSQFVSSM SASADVLAMS
160 170 180 190 200
KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT KKEIDQEAAV EVSQLRDPQH
210 220 230 240 250
DLERVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG SHYDASGRIR
260 270
KGPAPLNLEV PTYEFTSGDV VVVG
Length:274
Mass (Da):29,446
Last modified:September 13, 2004 - v2
Checksum:i6A0E0A07E956340B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY539946 mRNA Translation: AAS66286.1
BC085339 mRNA Translation: AAH85339.1
M24542 Genomic DNA Translation: AAA42051.1
PIRiA32296
RefSeqiNP_001008888.1, NM_001008888.1
UniGeneiRn.2603

Genome annotation databases

EnsembliENSRNOT00000024609; ENSRNOP00000024609; ENSRNOG00000018281
GeneIDi291103
KEGGirno:291103
UCSCiRGD:628838 rat

Similar proteinsi

Entry informationi

Entry nameiUCRI_RAT
AccessioniPrimary (citable) accession number: P20788
Secondary accession number(s): Q6QI13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: September 13, 2004
Last modified: April 25, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health