Reviewed, UniProtKB/Swiss-Prot P20788 (UCRI_RAT)

Names and origin

Protein names

Recommended name:
    Cytochrome b-c1 complex subunit Rieske, mitochondrial
    EC=1.10.2.2
Alternative name(s):
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    Rieske iron-sulfur protein
      Short name=RISP
    Complex III subunit 5
    Liver regeneration-related protein LRRGT00195
Cleaved into the following chain:
    1- Recommended name:
            Cytochrome b-c1 complex subunit 11
        Alternative name(s):
            Ubiquinol-cytochrome c reductase 8 kDa protein
            Complex III subunit IX

Gene names

Name:

Uqcrfs1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex By similarity.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) By similarity.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from expression pattern. Source: RGD response to drug Inferred from expression pattern. Source: RGD response to hormone stimulus Inferred from expression pattern. Source: RGD transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain complex III Inferred from direct assay. Source: RGD
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein complex binding Inferred from direct assay. Source: RGD ubiquinol-cytochrome-c reductase activity Ref.3 Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 78	78	Cytochrome b-c1 complex subunit 11 By similarity		PRO_0000307248
	Transit peptide	1 – 78	78	Mitochondrion By similarity
	Chain	79 – 274	196	Cytochrome b-c1 complex subunit Rieske, mitochondrial		PRO_0000030671
Regions
	Domain	187 – 272	86	Rieske
Sites
	Metal binding	217	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	219	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	236	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	239	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	222 ↔ 238		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20788-1 [UniParc]. Last modified September 13, 2004. Version 2. Checksum: 6A0E0A07E956340B	FASTA	274	29,446
	10 20 30 40 50 60 MLSVAARSGP FAPVLSATSR GVAGALRPLL QSAVPATSEP PVLDVKRPFL CRESLSGQAA 70 80 90 100 110 120 TRPLVATVGL NVPASVRYSH TDIKVPDFSD YRRAEVLDST KSSKESSEAR KGFSYLVTAT 130 140 150 160 170 180 TTVGVAYAAK NAVSQFVSSM SASADVLAMS KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT 190 200 210 220 230 240 KKEIDQEAAV EVSQLRDPQH DLERVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG 250 260 270 SHYDASGRIR KGPAPLNLEV PTYEFTSGDV VVVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Liver regeneration after PH." Xu C.S., Zhang L., Chang C.F., Han H.P., Wang G.P., Chai L.Q., Yuan J.Y., Yang K.J., Zhao L.F., Ma H., Wang L., Wang S.F., Xing X.K., Shen G.M., Shi J.B., Rahman S., Wang Q.N., Zhang J.B. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[3]	"Cloning and sequence analysis of a cDNA encoding the Rieske iron-sulfur protein of rat mitochondrial cytochrome bc1 complex." Nishikimi M., Hosokawa Y., Toda H., Suzuki H., Ozawa T. Biochem. Biophys. Res. Commun. 159:19-25(1989) [PubMed: 2538119] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-274.
[4]	Lubec G., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 85-92 AND 156-163, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY539946 mRNA. Translation: AAS66286.1. BC085339 mRNA. Translation: AAH85339.1. M24542 Genomic DNA. Translation: AAA42051.1.
IPI	IPI00362949.
PIR	A32296.
RefSeq	NP_001008888.1.
UniGene	Rn.2603
3D structure databases
HSSP	HSSP built from PDB template 1RIE based on UniProtKB P13272.
SMR	P20788. Positions 1-57, 79-274.
ModBase	Search...
2-D gel databases
Rat-heart-2DPAGE	P20788.
Proteomic databases
PRIDE	P20788.
Genome annotation databases
Ensembl	ENSRNOG00000018281. Rattus norvegicus. [Contig view]
GeneID	291103.
KEGG	rno:291103.
NMPDR	fig|10116.3.peg.13264.
Organism-specific databases
RGD	628838. Uqcrfs1.
Phylogenomic databases
HOVERGEN	P20788.
OMA	P20788. DYRRAEV.
Enzyme and pathway databases
BRENDA	1.10.2.2. 248.
Gene expression databases
ArrayExpress	P20788.
GermOnline	ENSRNOG00000018281. Rattus norvegicus.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR015248. Ubiqinol_cyt_c_Rdtase_N. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. IPR004192. Ubiquinol_cyt_Rdtase_TM. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF09165. Ubiq-Cytc-red_N. 1 hit. PF02921. UCR_TM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	632121.

Entry information

Entry name

UCRI_RAT

Accession

Primary (citable) accession number: P20788
Secondary accession number(s): Q6QI13

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	September 13, 2004
Last modified:	June 16, 2009
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)