ID   NTF3_HUMAN              Reviewed;         257 AA.
AC   P20783; Q6FH50;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   24-NOV-2009, entry version 110.
DE   RecName: Full=Neurotrophin-3;
DE            Short=NT-3;
DE   AltName: Full=Neurotrophic factor;
DE   AltName: Full=HDNF;
DE   AltName: Full=Nerve growth factor 2;
DE            Short=NGF-2;
DE   Flags: Precursor;
GN   Name=NTF3;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=90306351; PubMed=2365067; DOI=10.1016/0014-5793(90)81536-W;
RA   Kaisho Y., Yoshimura K., Nakahama K.;
RT   "Cloning and expression of a cDNA encoding a novel human neurotrophic
RT   factor.";
RL   FEBS Lett. 266:187-191(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX   MEDLINE=90262727; PubMed=2344409; DOI=10.1016/0896-6273(90)90203-R;
RA   Rosenthal A., Goeddel D.V., Nguyen T., Lewis M., Shih A.,
RA   Laramee G.R., Nikolics K., Winslow J.W.;
RT   "Primary structure and biological activity of a novel human
RT   neurotrophic factor.";
RL   Neuron 4:767-773(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=91045937; PubMed=2236018; DOI=10.1073/pnas.87.20.8060;
RA   Jones K.R., Reichardt L.F.;
RT   "Molecular cloning of a human gene that is a member of the nerve
RT   growth factor family.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:8060-8064(1990).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=91365361; PubMed=1889806; DOI=10.1016/0888-7543(91)90436-I;
RA   Maisonpierre P.C., le Beau M.M., Espinosa R. III, Ip N.Y.,
RA   Belluscio L., de la Monte S.M., Squinto S., Furth M.E.,
RA   Yancopoulos G.D.;
RT   "Human and rat brain-derived neurotrophic factor and neurotrophin-3:
RT   gene structures, distributions, and chromosomal localizations.";
RL   Genomics 10:558-568(1991).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
RA   Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
RA   Korn B., Zuo D., Hu Y., LaBaer J.;
RT   "Cloning of human full open reading frames in Gateway(TM) system entry
RT   vector (pDONR201).";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [8]
RP   NUCLEOTIDE SEQUENCE OF 194-236.
RC   TISSUE=Leukocyte;
RX   MEDLINE=91222573; PubMed=2025430; DOI=10.1016/0896-6273(91)90180-8;
RA   Hallboeoek F., Ibanez C.F., Persson H.;
RT   "Evolutionary studies of the nerve growth factor family reveal a novel
RT   member abundantly expressed in Xenopus ovary.";
RL   Neuron 6:845-858(1991).
RN   [9]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RX   MEDLINE=95217877; PubMed=7703225; DOI=10.1021/bi00013a001;
RA   Robinson R.C., Radziejewski C., Stuart D.I., Jones E.Y.;
RT   "Structure of the brain-derived neurotrophic factor/neurotrophin 3
RT   heterodimer.";
RL   Biochemistry 34:4139-4146(1995).
RN   [10]
RP   VARIANT GLU-76.
RX   MEDLINE=95251647; PubMed=7733919; DOI=10.1006/bbrc.1995.1531;
RA   Hattori M., Nanko S.;
RT   "Association of neurotrophin-3 gene variant with severe forms of
RT   schizophrenia.";
RL   Biochem. Biophys. Res. Commun. 209:513-518(1995).
RN   [11]
RP   VARIANT GLU-76.
RX   MEDLINE=96253892; PubMed=8925252;
RA   Arinami T., Takekoshi K., Itokawa M., Hamaguchi H., Toru M.;
RT   "Failure to find associations of the CA repeat polymorphism in the
RT   first intron and the Gly-63/Glu-63 polymorphism of the neurotrophin-3
RT   gene with schizophrenia.";
RL   Psychiatr. Genet. 6:13-15(1996).
CC   -!- FUNCTION: Seems to promotes the survival of visceral and
CC       proprioceptive sensory neurons.
CC   -!- INTERACTION:
CC       P23560:BDNF; NbExp=1; IntAct=EBI-1025994, EBI-1026003;
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Brain and peripheral tissues.
CC   -!- POLYMORPHISM: Variant Glu-76 (frequently reported as Glu-63) was
CC       thought to be associated with severe forms of schizophrenia. This
CC       does not seem to be the case.
CC   -!- SIMILARITY: Belongs to the NGF-beta family.
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DR   EMBL; X53655; CAA37703.1; -; mRNA.
DR   EMBL; M37763; AAA59953.1; -; Genomic_DNA.
DR   EMBL; M61180; AAA63231.1; -; Genomic_DNA.
DR   EMBL; CR541906; CAG46704.1; -; mRNA.
DR   EMBL; CH471116; EAW88824.1; -; Genomic_DNA.
DR   EMBL; BC069773; AAH69773.1; -; mRNA.
DR   EMBL; BC107075; AAI07076.1; -; mRNA.
DR   IPI; IPI00025474; -.
DR   PIR; A36208; C40304.
DR   RefSeq; NP_001096124.1; -.
DR   RefSeq; NP_002518.1; -.
DR   UniGene; Hs.99171; -.
DR   PDB; 1B8K; X-ray; 2.15 A; A=139-257.
DR   PDB; 1BND; X-ray; 2.30 A; B=139-257.
DR   PDB; 1NT3; X-ray; 2.40 A; A=139-257.
DR   PDB; 3BUK; X-ray; 2.60 A; A/B=139-257.
DR   PDBsum; 1B8K; -.
DR   PDBsum; 1BND; -.
DR   PDBsum; 1NT3; -.
DR   PDBsum; 3BUK; -.
DR   DIP; DIP:346N; -.
DR   IntAct; P20783; 1.
DR   STRING; P20783; -.
DR   PRIDE; P20783; -.
DR   Ensembl; ENST00000331010; ENSP00000328738; ENSG00000185652; Homo sapiens.
DR   Ensembl; ENST00000423158; ENSP00000397297; ENSG00000185652; Homo sapiens.
DR   GeneID; 4908; -.
DR   KEGG; hsa:4908; -.
DR   UCSC; uc001qnk.2; human.
DR   CTD; 4908; -.
DR   GeneCards; GC12P005473; -.
DR   HGNC; HGNC:8023; NTF3.
DR   MIM; 162660; gene.
DR   PharmGKB; PA31806; -.
DR   HOGENOM; P20783; -.
DR   HOVERGEN; P20783; -.
DR   Pathway_Interaction_DB; trkrpathway; Neurotrophic factor-mediated Trk receptor signaling.
DR   Pathway_Interaction_DB; p75ntrpathway; p75(NTR)-mediated signaling.
DR   Pathway_Interaction_DB; mapktrkpathway; Trk receptor signaling mediated by the MAPK pathway.
DR   NextBio; 18887; -.
DR   PMAP-CutDB; P20783; -.
DR   ArrayExpress; P20783; -.
DR   Bgee; P20783; -.
DR   CleanEx; HS_NTF3; -.
DR   Genevestigator; P20783; -.
DR   GermOnline; ENSG00000185652; Homo sapiens.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0005165; F:neurotrophin receptor binding; IEA:InterPro.
DR   GO; GO:0007165; P:signal transduction; TAS:ProtInc.
DR   InterPro; IPR020408; Nerve_growth_factor-like.
DR   InterPro; IPR002072; Nerve_growth_factor-rel.
DR   InterPro; IPR019846; Nerve_growth_factor_CS.
DR   InterPro; IPR015578; Neurotrophin-3.
DR   InterPro; IPR020409; Neurotrophin-3_N.
DR   PANTHER; PTHR11589:SF4; Neurotrophin-3; 1.
DR   PANTHER; PTHR11589; NGF; 1.
DR   Pfam; PF00243; NGF; 1.
DR   PIRSF; PIRSF001789; NGF; 1.
DR   PRINTS; PR01914; NEUROTROPHN3.
DR   PRINTS; PR00268; NGF.
DR   ProDom; PD002052; Nerve_growth_factor-rel; 1.
DR   SMART; SM00140; NGF; 1.
DR   PROSITE; PS00248; NGF_1; 1.
DR   PROSITE; PS50270; NGF_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cleavage on pair of basic residues; Complete proteome;
KW   Disulfide bond; Glycoprotein; Growth factor; Polymorphism; Secreted;
KW   Signal.
FT   SIGNAL        1     18       Potential.
FT   PROPEP       19    138
FT                                /FTId=PRO_0000019659.
FT   CHAIN       139    257       Neurotrophin-3.
FT                                /FTId=PRO_0000019660.
FT   CARBOHYD    131    131       N-linked (GlcNAc...) (Potential).
FT   DISULFID    152    217
FT   DISULFID    195    246
FT   DISULFID    205    248
FT   VARIANT      76     76       G -> E (in dbSNP:rs1805149).
FT                                /FTId=VAR_012084.
FT   STRAND      150    152
FT   STRAND      154    159
FT   STRAND      164    167
FT   STRAND      172    175
FT   STRAND      190    195
FT   STRAND      216    233
FT   STRAND      236    249
SQ   SEQUENCE   257 AA;  29355 MW;  39A5BB3B28E25E03 CRC64;
     MSILFYVIFL AYLRGIQGNN MDQRSLPEDS LNSLIIKLIQ ADILKNKLSK QMVDVKENYQ
     STLPKAEAPR EPERGGPAKS AFQPVIAMDT ELLRQQRRYN SPRVLLSDST PLEPPPLYLM
     EDYVGSPVVA NRTSRRKRYA EHKSHRGEYS VCDSESLWVT DKSSAIDIRG HQVTVLGEIK
     TGNSPVKQYF YETRCKEARP VKNGCRGIDD KHWNSQCKTS QTYVRALTSE NNKLVGWRWI
     RIDTSCVCAL SRKIGRT
//