Reviewed, UniProtKB/Swiss-Prot P20783 (NTF3_HUMAN)

Names and origin

Protein names

Recommended name:
    Neurotrophin-3
      Short name=NT-3
Alternative name(s):
    Neurotrophic factor
    HDNF
    Nerve growth factor 2
      Short name=NGF-2

Gene names

Name:

NTF3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Seems to promotes the survival of visceral and proprioceptive sensory neurons.
Subcellular location	Secreted.
Tissue specificity	Brain and peripheral tissues.
Polymorphism	Variant Glu-76 (frequently reported as Glu-63) was thought to be associated with severe forms of schizophrenia. This does not seem to be the case.
Sequence similarities	Belongs to the NGF-beta family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Growth factor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	signal transduction Ref.3 Traceable author statement. Source: ProtInc
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW neurotrophin receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BDNF	P23560	1	EBI-1025994,EBI-1026003

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Potential

Propeptide

19 – 138

120

PRO_0000019659

Chain

139 – 257

119

Neurotrophin-3

PRO_0000019660

Amino acid modifications

Glycosylation

131

1

N-linked (GlcNAc...) Potential

Disulfide bond

152 ↔ 217

Disulfide bond

195 ↔ 246

Disulfide bond

205 ↔ 248

Natural variations

Natural variant

76

1

G → E: dbSNP rs1805149. Ref.10 Ref.11

VAR_012084

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

257

Helix Strand Turn

Details...

Beta strand

150 – 152

3

Beta strand

154 – 159

6

Beta strand

164 – 167

4

Beta strand

172 – 175

4

Beta strand

190 – 195

6

Beta strand

216 – 233

18

Beta strand

236 – 249

14

Sequences

	Sequence		Length	Mass (Da)
	P20783-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 39A5BB3B28E25E03	FASTA	257	29,355
	10 20 30 40 50 60 MSILFYVIFL AYLRGIQGNN MDQRSLPEDS LNSLIIKLIQ ADILKNKLSK QMVDVKENYQ 70 80 90 100 110 120 STLPKAEAPR EPERGGPAKS AFQPVIAMDT ELLRQQRRYN SPRVLLSDST PLEPPPLYLM 130 140 150 160 170 180 EDYVGSPVVA NRTSRRKRYA EHKSHRGEYS VCDSESLWVT DKSSAIDIRG HQVTVLGEIK 190 200 210 220 230 240 TGNSPVKQYF YETRCKEARP VKNGCRGIDD KHWNSQCKTS QTYVRALTSE NNKLVGWRWI 250 RIDTSCVCAL SRKIGRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a cDNA encoding a novel human neurotrophic factor." Kaisho Y., Yoshimura K., Nakahama K. FEBS Lett. 266:187-191(1990) [PubMed: 2365067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Primary structure and biological activity of a novel human neurotrophic factor." Rosenthal A., Goeddel D.V., Nguyen T., Lewis M., Shih A., Laramee G.R., Nikolics K., Winslow J.W. Neuron 4:767-773(1990) [PubMed: 2344409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Molecular cloning of a human gene that is a member of the nerve growth factor family." Jones K.R., Reichardt L.F. Proc. Natl. Acad. Sci. U.S.A. 87:8060-8064(1990) [PubMed: 2236018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Human and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions, and chromosomal localizations." Maisonpierre P.C., le Beau M.M., Espinosa R. III, Ip N.Y., Belluscio L., de la Monte S.M., Squinto S., Furth M.E., Yancopoulos G.D. Genomics 10:558-568(1991) [PubMed: 1889806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary." Hallboeoek F., Ibanez C.F., Persson H. Neuron 6:845-858(1991) [PubMed: 2025430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 194-236. Tissue: Leukocyte.
[9]	"Structure of the brain-derived neurotrophic factor/neurotrophin 3 heterodimer." Robinson R.C., Radziejewski C., Stuart D.I., Jones E.Y. Biochemistry 34:4139-4146(1995) [PubMed: 7703225] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[10]	"Association of neurotrophin-3 gene variant with severe forms of schizophrenia." Hattori M., Nanko S. Biochem. Biophys. Res. Commun. 209:513-518(1995) [PubMed: 7733919] [Abstract] Cited for: VARIANT GLU-76.
[11]	"Failure to find associations of the CA repeat polymorphism in the first intron and the Gly-63/Glu-63 polymorphism of the neurotrophin-3 gene with schizophrenia." Arinami T., Takekoshi K., Itokawa M., Hamaguchi H., Toru M. Psychiatr. Genet. 6:13-15(1996) [PubMed: 8925252] [Abstract] Cited for: VARIANT GLU-76.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X53655 mRNA. Translation: CAA37703.1.
M37763 Genomic DNA. Translation: AAA59953.1.
M61180 Genomic DNA. Translation: AAA63231.1.
CR541906 mRNA. Translation: CAG46704.1.
CH471116 Genomic DNA. Translation: EAW88824.1.
BC069773 mRNA. Translation: AAH69773.1.
BC107075 mRNA. Translation: AAI07076.1.

IPI

IPI00025474.

PIR

C40304. A36208.

RefSeq

NP_001096124.1.
NP_002518.1.

UniGene

Hs.99171

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B8K	X-ray	2.15	A	139-257	[»]
1BND	X-ray	2.30	B	139-257	[»]
1NT3	X-ray	2.40	A	139-257	[»]
3BUK	X-ray	2.60	A/B	139-257	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:346N.

IntAct

P20783. 1 interaction.

Proteomic databases

PRIDE

P20783.

Genome annotation databases

Ensembl

ENSG00000185652. Homo sapiens. [Contig view]

GeneID

4908.

KEGG

hsa:4908.

Organism-specific databases

GeneCards

GC12P005473.

HGNC

HGNC:8023. NTF3.

MIM

162660. gene.

PharmGKB

PA31806.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P20783.

HOVERGEN

P20783.

OMA

P20783. QRRYSSP.

Enzyme and pathway databases

Pathway_Interaction_DB

trkrpathway. Neurotrophic factor-mediated Trk receptor signaling.
p75ntrpathway. p75(NTR)-mediated signaling.
mapktrkpathway. Trk receptor signaling mediated by the MAPK pathway.

Gene expression databases

ArrayExpress

P20783.

Bgee

P20783.

CleanEx

HS_NTF3.

GermOnline

ENSG00000185652. Homo sapiens.

Family and domain databases

InterPro

IPR002072. Nerve_growth_factor-rel.
IPR019846. Nerve_growth_factor_CS.
IPR015578. Neurotrophin-3.
[Graphical view]

PANTHER

PTHR11589:SF4. Neurotrophin-3. 1 hit.
PTHR11589. NGF. 1 hit.

Pfam

PF00243. NGF. 1 hit.
[Graphical view]

PIRSF

PIRSF001789. NGF. 1 hit.

PRINTS

PR00268. NGF.

ProDom

PD002052. NGF. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00140. NGF. 1 hit.
[Graphical view]

PROSITE

PS00248. NGF_1. 1 hit.
PS50270. NGF_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

18887.

PMAP-CutDB

P20783.

SOURCE

Search...

Entry information

Entry name

NTF3_HUMAN

Accession

Primary (citable) accession number: P20783
Secondary accession number(s): Q6FH50

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)