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P20783 (NTF3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 150. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neurotrophin-3

Short name=NT-3
Alternative name(s):
HDNF
Nerve growth factor 2
Short name=NGF-2
Neurotrophic factor
Gene names
Name:NTF3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Seems to promote the survival of visceral and proprioceptive sensory neurons.

Subcellular location

Secreted.

Tissue specificity

Brain and peripheral tissues.

Polymorphism

Variant Glu-76 (frequently reported as Glu-63) was thought to be associated with severe forms of schizophrenia. This does not seem to be the case.

Sequence similarities

Belongs to the NGF-beta family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   Molecular functionGrowth factor
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of MAPK activity

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

activation of Ras GTPase activity

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

activation of protein kinase B activity

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

axon guidance

Inferred from electronic annotation. Source: Ensembl

brain development

Inferred from electronic annotation. Source: Ensembl

cell-cell signaling

Traceable author statement Ref.3. Source: ProtInc

enteric nervous system development

Inferred from electronic annotation. Source: Ensembl

epidermis development

Inferred from electronic annotation. Source: Ensembl

glial cell fate determination

Inferred from electronic annotation. Source: Ensembl

induction of positive chemotaxis

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

mechanoreceptor differentiation

Inferred from electronic annotation. Source: Ensembl

myelination

Inferred from electronic annotation. Source: Ensembl

negative regulation of neuron apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

nerve development

Inferred from electronic annotation. Source: Ensembl

nervous system development

Traceable author statement PubMed 1542658. Source: ProtInc

neuromuscular synaptic transmission

Inferred from electronic annotation. Source: Ensembl

peripheral nervous system development

Inferred from electronic annotation. Source: Ensembl

positive chemotaxis

Inferred from direct assay PubMed 23027130. Source: GOC

positive regulation of actin cytoskeleton reorganization

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

positive regulation of cell migration

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

positive regulation of cell proliferation

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

positive regulation of glial cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-serine phosphorylation

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

positive regulation of receptor internalization

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

regulation of synaptic transmission

Inferred from electronic annotation. Source: Ensembl

signal transduction

Traceable author statement Ref.3. Source: ProtInc

smooth muscle cell differentiation

Inferred from electronic annotation. Source: Ensembl

transmembrane receptor protein tyrosine kinase signaling pathway

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

   Cellular_componentcytoplasmic membrane-bounded vesicle

Inferred from electronic annotation. Source: Ensembl

extracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionchemoattractant activity

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

growth factor activity

Inferred from direct assay PubMed 23027130. Source: BHF-UCL

receptor binding

Traceable author statement Ref.3. Source: ProtInc

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BDNFP235601EBI-1025994,EBI-1026003

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P20783-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P20783-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVTFATILQVNKVM
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Propeptide19 – 138120
PRO_0000019659
Chain139 – 257119Neurotrophin-3
PRO_0000019660

Amino acid modifications

Glycosylation1311N-linked (GlcNAc...) Potential
Disulfide bond152 ↔ 217
Disulfide bond195 ↔ 246
Disulfide bond205 ↔ 248

Natural variations

Alternative sequence11M → MVTFATILQVNKVM in isoform 2.
VSP_043353
Natural variant761G → E. Ref.11 Ref.12
Corresponds to variant rs1805149 [ dbSNP | Ensembl ].
VAR_012084

Secondary structure

....................... 257
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 39A5BB3B28E25E03

FASTA25729,355
        10         20         30         40         50         60 
MSILFYVIFL AYLRGIQGNN MDQRSLPEDS LNSLIIKLIQ ADILKNKLSK QMVDVKENYQ 

        70         80         90        100        110        120 
STLPKAEAPR EPERGGPAKS AFQPVIAMDT ELLRQQRRYN SPRVLLSDST PLEPPPLYLM 

       130        140        150        160        170        180 
EDYVGSPVVA NRTSRRKRYA EHKSHRGEYS VCDSESLWVT DKSSAIDIRG HQVTVLGEIK 

       190        200        210        220        230        240 
TGNSPVKQYF YETRCKEARP VKNGCRGIDD KHWNSQCKTS QTYVRALTSE NNKLVGWRWI 

       250 
RIDTSCVCAL SRKIGRT 

« Hide

Isoform 2 [UniParc].

Checksum: 43B3342B32424E97
Show »

FASTA27030,800

References

« Hide 'large scale' references
[1]"Cloning and expression of a cDNA encoding a novel human neurotrophic factor."
Kaisho Y., Yoshimura K., Nakahama K.
FEBS Lett. 266:187-191(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Primary structure and biological activity of a novel human neurotrophic factor."
Rosenthal A., Goeddel D.V., Nguyen T., Lewis M., Shih A., Laramee G.R., Nikolics K., Winslow J.W.
Neuron 4:767-773(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
[3]"Molecular cloning of a human gene that is a member of the nerve growth factor family."
Jones K.R., Reichardt L.F.
Proc. Natl. Acad. Sci. U.S.A. 87:8060-8064(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Human and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions, and chromosomal localizations."
Maisonpierre P.C., le Beau M.M., Espinosa R. III, Ip N.Y., Belluscio L., de la Monte S.M., Squinto S., Furth M.E., Yancopoulos G.D.
Genomics 10:558-568(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Cerebellum.
[6]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[9]"Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary."
Hallboeoek F., Ibanez C.F., Persson H.
Neuron 6:845-858(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 194-236.
Tissue: Leukocyte.
[10]"Structure of the brain-derived neurotrophic factor/neurotrophin 3 heterodimer."
Robinson R.C., Radziejewski C., Stuart D.I., Jones E.Y.
Biochemistry 34:4139-4146(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[11]"Association of neurotrophin-3 gene variant with severe forms of schizophrenia."
Hattori M., Nanko S.
Biochem. Biophys. Res. Commun. 209:513-518(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT GLU-76.
[12]"Failure to find associations of the CA repeat polymorphism in the first intron and the Gly-63/Glu-63 polymorphism of the neurotrophin-3 gene with schizophrenia."
Arinami T., Takekoshi K., Itokawa M., Hamaguchi H., Toru M.
Psychiatr. Genet. 6:13-15(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT GLU-76.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53655 mRNA. Translation: CAA37703.1.
M37763 Genomic DNA. Translation: AAA59953.1.
M61180 Genomic DNA. Translation: AAA63231.1.
AK293895 mRNA. Translation: BAH11621.1.
CR541906 mRNA. Translation: CAG46704.1.
CH471116 Genomic DNA. Translation: EAW88824.1.
CH471116 Genomic DNA. Translation: EAW88825.1.
BC069773 mRNA. Translation: AAH69773.1.
BC107075 mRNA. Translation: AAI07076.1.
CCDSCCDS44806.1. [P20783-2]
CCDS8538.1. [P20783-1]
PIRC40304. A36208.
RefSeqNP_001096124.1. NM_001102654.1. [P20783-2]
NP_002518.1. NM_002527.4. [P20783-1]
UniGeneHs.99171.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B8KX-ray2.15A139-257[»]
1BNDX-ray2.30B139-257[»]
1NT3X-ray2.40A139-257[»]
3BUKX-ray2.60A/B139-257[»]
ProteinModelPortalP20783.
SMRP20783. Positions 146-253.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110963. 5 interactions.
DIPDIP-346N.
IntActP20783. 1 interaction.
MINTMINT-188565.
STRING9606.ENSP00000397297.

PTM databases

PhosphoSiteP20783.

Polymorphism databases

DMDM128581.

Proteomic databases

PaxDbP20783.
PRIDEP20783.

Protocols and materials databases

DNASU4908.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000331010; ENSP00000328738; ENSG00000185652. [P20783-1]
ENST00000423158; ENSP00000397297; ENSG00000185652. [P20783-2]
GeneID4908.
KEGGhsa:4908.
UCSCuc001qnk.4. human. [P20783-2]
uc001qnl.4. human. [P20783-1]

Organism-specific databases

CTD4908.
GeneCardsGC12P005541.
HGNCHGNC:8023. NTF3.
HPAHPA032000.
MIM162660. gene.
neXtProtNX_P20783.
PharmGKBPA31806.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG39844.
HOGENOMHOG000231516.
HOVERGENHBG006494.
InParanoidP20783.
KOK04356.
OMAYAEHKSH.
OrthoDBEOG7RBZ8Z.
PhylomeDBP20783.
TreeFamTF106463.

Enzyme and pathway databases

SignaLinkP20783.

Gene expression databases

BgeeP20783.
CleanExHS_NTF3.
GenevestigatorP20783.

Family and domain databases

Gene3D2.10.90.10. 1 hit.
InterProIPR029034. Cystine-knot_cytokine.
IPR020408. Nerve_growth_factor-like.
IPR002072. Nerve_growth_factor-rel.
IPR019846. Nerve_growth_factor_CS.
IPR015578. Neurotrophin-3.
[Graphical view]
PANTHERPTHR11589. PTHR11589. 1 hit.
PTHR11589:SF4. PTHR11589:SF4. 1 hit.
PfamPF00243. NGF. 1 hit.
[Graphical view]
PIRSFPIRSF001789. NGF. 1 hit.
PRINTSPR01914. NEUROTROPHN3.
PR00268. NGF.
ProDomPD002052. Nerve_growth_factor-rel. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00140. NGF. 1 hit.
[Graphical view]
SUPFAMSSF57501. SSF57501. 1 hit.
PROSITEPS00248. NGF_1. 1 hit.
PS50270. NGF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP20783.
GeneWikiNeurotrophin-3.
GenomeRNAi4908.
NextBio18887.
PMAP-CutDBP20783.
PROP20783.
SOURCESearch...

Entry information

Entry nameNTF3_HUMAN
AccessionPrimary (citable) accession number: P20783
Secondary accession number(s): B7Z1T5, Q6FH50
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM