ID   ACHE_MOUSE              Reviewed;         493 AA.
AC   P20782;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   27-MAR-2024, entry version 176.
DE   RecName: Full=Acetylcholine receptor subunit epsilon;
DE   Flags: Precursor;
GN   Name=Chrne; Synonyms=Acre;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2708381; DOI=10.1016/s0021-9258(18)83278-1;
RA   Buonanno A., Mudd J., Merlie J.P.;
RT   "Isolation and characterization of the beta and epsilon subunit genes of
RT   mouse muscle acetylcholine receptor.";
RL   J. Biol. Chem. 264:7611-7616(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2251144; DOI=10.1093/nar/18.22.6714;
RA   Gardner P.D.;
RT   "Nucleotide sequence of the epsilon-subunit of the mouse muscle nicotinic
RT   acetylcholine receptor.";
RL   Nucleic Acids Res. 18:6714-6714(1990).
CC   -!- FUNCTION: After binding acetylcholine, the AChR responds by an
CC       extensive change in conformation that affects all subunits and leads to
CC       opening of an ion-conducting channel across the plasma membrane.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=K(+)(in) = K(+)(out); Xref=Rhea:RHEA:29463, ChEBI:CHEBI:29103;
CC         Evidence={ECO:0000250|UniProtKB:P02715};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Na(+)(in) = Na(+)(out); Xref=Rhea:RHEA:34963,
CC         ChEBI:CHEBI:29101; Evidence={ECO:0000250|UniProtKB:P02715};
CC   -!- SUBUNIT: Pentamer of two alpha chains, and one each of the beta, delta,
CC       and gamma (in immature muscle) or epsilon (in mature muscle) chains.
CC       The muscle heteropentamer composed of alpha-1, beta-1, delta, epsilon
CC       subunits interacts with the alpha-conotoxin ImII (By similarity).
CC       {ECO:0000250|UniProtKB:Q04844}.
CC   -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane
CC       protein. Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC       Acetylcholine receptor (TC 1.A.9.1) subfamily. Epsilon/CHRNE sub-
CC       subfamily. {ECO:0000305}.
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DR   EMBL; J04698; AAA37153.1; -; Genomic_DNA.
DR   EMBL; X55718; CAA39251.1; -; mRNA.
DR   CCDS; CCDS24956.1; -.
DR   PIR; S13592; ACMSE.
DR   RefSeq; NP_033733.1; NM_009603.1.
DR   AlphaFoldDB; P20782; -.
DR   SMR; P20782; -.
DR   ComplexPortal; CPX-257; Muscle-type nicotinic acetylcholine receptor complex, alpha1-beta1-delta-epsilon.
DR   STRING; 10090.ENSMUSP00000099616; -.
DR   BindingDB; P20782; -.
DR   ChEMBL; CHEMBL3091265; -.
DR   GlyCosmos; P20782; 2 sites, No reported glycans.
DR   GlyGen; P20782; 2 sites.
DR   PhosphoSitePlus; P20782; -.
DR   SwissPalm; P20782; -.
DR   PaxDb; 10090-ENSMUSP00000099616; -.
DR   ProteomicsDB; 285976; -.
DR   Antibodypedia; 11366; 203 antibodies from 28 providers.
DR   DNASU; 11448; -.
DR   Ensembl; ENSMUST00000102556.10; ENSMUSP00000099616.4; ENSMUSG00000014609.17.
DR   GeneID; 11448; -.
DR   KEGG; mmu:11448; -.
DR   UCSC; uc007jvo.1; mouse.
DR   AGR; MGI:87894; -.
DR   CTD; 1145; -.
DR   MGI; MGI:87894; Chrne.
DR   VEuPathDB; HostDB:ENSMUSG00000014609; -.
DR   eggNOG; KOG3645; Eukaryota.
DR   GeneTree; ENSGT00940000160933; -.
DR   HOGENOM; CLU_018074_1_4_1; -.
DR   InParanoid; P20782; -.
DR   OMA; PFPPCIQ; -.
DR   OrthoDB; 5489962at2759; -.
DR   TreeFam; TF315605; -.
DR   Reactome; R-MMU-629587; Highly sodium permeable postsynaptic acetylcholine nicotinic receptors.
DR   BioGRID-ORCS; 11448; 2 hits in 77 CRISPR screens.
DR   PRO; PR:P20782; -.
DR   Proteomes; UP000000589; Chromosome 11.
DR   RNAct; P20782; Protein.
DR   Bgee; ENSMUSG00000014609; Expressed in spermatid and 37 other cell types or tissues.
DR   ExpressionAtlas; P20782; baseline and differential.
DR   GO; GO:0005892; C:acetylcholine-gated channel complex; ISO:MGI.
DR   GO; GO:0031594; C:neuromuscular junction; IDA:SynGO.
DR   GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR   GO; GO:0045211; C:postsynaptic membrane; IDA:MGI.
DR   GO; GO:0099634; C:postsynaptic specialization membrane; IDA:SynGO.
DR   GO; GO:0045202; C:synapse; IBA:GO_Central.
DR   GO; GO:0022848; F:acetylcholine-gated monoatomic cation-selective channel activity; ISO:MGI.
DR   GO; GO:0005231; F:excitatory extracellular ligand-gated monoatomic ion channel activity; IBA:GO_Central.
DR   GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR   GO; GO:1904315; F:transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential; ISO:MGI.
DR   GO; GO:0006812; P:monoatomic cation transport; ISO:MGI.
DR   GO; GO:0042391; P:regulation of membrane potential; IGI:MGI.
DR   GO; GO:0003009; P:skeletal muscle contraction; ISO:MGI.
DR   CDD; cd19030; LGIC_ECD_nAChR_E; 1.
DR   CDD; cd19064; LGIC_TM_nAChR; 1.
DR   Gene3D; 2.70.170.10; Neurotransmitter-gated ion-channel ligand-binding domain; 1.
DR   Gene3D; 1.20.58.390; Neurotransmitter-gated ion-channel transmembrane domain; 2.
DR   InterPro; IPR006202; Neur_chan_lig-bd.
DR   InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR   InterPro; IPR006201; Neur_channel.
DR   InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR   InterPro; IPR038050; Neuro_actylchol_rec.
DR   InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR   InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR   InterPro; IPR002394; Nicotinic_acetylcholine_rcpt.
DR   PANTHER; PTHR18945:SF59; ACETYLCHOLINE RECEPTOR SUBUNIT EPSILON; 1.
DR   PANTHER; PTHR18945; NEUROTRANSMITTER GATED ION CHANNEL; 1.
DR   Pfam; PF02931; Neur_chan_LBD; 1.
DR   Pfam; PF02932; Neur_chan_memb; 1.
DR   PRINTS; PR00254; NICOTINICR.
DR   PRINTS; PR00252; NRIONCHANNEL.
DR   SUPFAM; SSF90112; Neurotransmitter-gated ion-channel transmembrane pore; 1.
DR   SUPFAM; SSF63712; Nicotinic receptor ligand binding domain-like; 1.
DR   PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
DR   Genevisible; P20782; MM.
PE   2: Evidence at transcript level;
KW   Cell membrane; Disulfide bond; Glycoprotein; Ion channel; Ion transport;
KW   Ligand-gated ion channel; Membrane; Postsynaptic cell membrane; Receptor;
KW   Reference proteome; Signal; Synapse; Transmembrane; Transmembrane helix;
KW   Transport.
FT   SIGNAL          1..20
FT   CHAIN           21..493
FT                   /note="Acetylcholine receptor subunit epsilon"
FT                   /id="PRO_0000000330"
FT   TOPO_DOM        21..239
FT                   /note="Extracellular"
FT   TRANSMEM        240..264
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        265..272
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        273..291
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        292..306
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        307..328
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        329..456
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        457..480
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        481..493
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        86
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        161
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        148..162
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   493 AA;  54914 MW;  F3E468644F3FB7DF CRC64;
     MAGALLGALL LLTLFGRSQG KNEELSLYHH LFDNYDPECR PVRRPEDTVT ITLKVTLTNL
     ISLNEKEETL TTSVWIGIDW HDYRLNYSKD DFAGVGILRV PSEHVWLPEI VLENNIDGQF
     GVAYDSNVLV YEGGYVSWLP PAIYRSTCAV EVTYFPFDWQ NCSLIFRSQT YNAEEVEFIF
     AVDDDGNTIN KIDIDTAAFT ENGEWAIDYC PGMIRRYEGG STEGPGETDV IYTLIIRRKP
     LFYVINIIVP CVLISGLVLL AYFLPAQAGG QKCTVSINVL LAQTVFLFLI AQKIPETSLS
     VPLLGRYLIF VMVVATLIVM NCVIVLNVSL RTPTTHATSP RLRQILLELL PRLLGSSPPP
     EDPRTASPAR RASSVGILLR AEELILKKPR SELVFEGQRH RHGTWTAALC QNLGAAAPEI
     RCCVDAVNFV AESTRDQEAT GEELSDWVRM GKALDNVCFW AALVLFSVGS TLIFLGGYFN
     QVPDLPYPPC IQP
//