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P20782 (ACHE_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetylcholine receptor subunit epsilon
Gene names
Name:Chrne
Synonyms:Acre
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Subunit structure

Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Epsilon/CHRNE sub-subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020
Chain21 – 493473Acetylcholine receptor subunit epsilon
PRO_0000000330

Regions

Topological domain21 – 239219Extracellular
Transmembrane240 – 26425Helical; Potential
Topological domain265 – 2728Cytoplasmic Potential
Transmembrane273 – 29119Helical; Potential
Topological domain292 – 30615Extracellular Potential
Transmembrane307 – 32822Helical; Potential
Topological domain329 – 456128Cytoplasmic Potential
Transmembrane457 – 48024Helical; Potential
Topological domain481 – 49313Extracellular Potential

Amino acid modifications

Glycosylation861N-linked (GlcNAc...) Potential
Glycosylation1611N-linked (GlcNAc...) Potential
Disulfide bond148 ↔ 162 By similarity

Sequences

Sequence LengthMass (Da)Tools
P20782 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: F3E468644F3FB7DF

FASTA49354,914
        10         20         30         40         50         60 
MAGALLGALL LLTLFGRSQG KNEELSLYHH LFDNYDPECR PVRRPEDTVT ITLKVTLTNL 

        70         80         90        100        110        120 
ISLNEKEETL TTSVWIGIDW HDYRLNYSKD DFAGVGILRV PSEHVWLPEI VLENNIDGQF 

       130        140        150        160        170        180 
GVAYDSNVLV YEGGYVSWLP PAIYRSTCAV EVTYFPFDWQ NCSLIFRSQT YNAEEVEFIF 

       190        200        210        220        230        240 
AVDDDGNTIN KIDIDTAAFT ENGEWAIDYC PGMIRRYEGG STEGPGETDV IYTLIIRRKP 

       250        260        270        280        290        300 
LFYVINIIVP CVLISGLVLL AYFLPAQAGG QKCTVSINVL LAQTVFLFLI AQKIPETSLS 

       310        320        330        340        350        360 
VPLLGRYLIF VMVVATLIVM NCVIVLNVSL RTPTTHATSP RLRQILLELL PRLLGSSPPP 

       370        380        390        400        410        420 
EDPRTASPAR RASSVGILLR AEELILKKPR SELVFEGQRH RHGTWTAALC QNLGAAAPEI 

       430        440        450        460        470        480 
RCCVDAVNFV AESTRDQEAT GEELSDWVRM GKALDNVCFW AALVLFSVGS TLIFLGGYFN 

       490 
QVPDLPYPPC IQP 

« Hide

References

[1]"Isolation and characterization of the beta and epsilon subunit genes of mouse muscle acetylcholine receptor."
Buonanno A., Mudd J., Merlie J.P.
J. Biol. Chem. 264:7611-7616(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequence of the epsilon-subunit of the mouse muscle nicotinic acetylcholine receptor."
Gardner P.D.
Nucleic Acids Res. 18:6714-6714(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04698 Genomic DNA. Translation: AAA37153.1.
X55718 mRNA. Translation: CAA39251.1.
CCDSCCDS24956.1.
PIRACMSE. S13592.
RefSeqNP_033733.1. NM_009603.1.
UniGeneMm.4980.

3D structure databases

ProteinModelPortalP20782.
SMRP20782. Positions 22-337, 448-485.
ModBaseSearch...
MobiDBSearch...

Chemistry

GuidetoPHARMACOLOGY477.

Proteomic databases

PaxDbP20782.
PRIDEP20782.

Protocols and materials databases

DNASU11448.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102556; ENSMUSP00000099616; ENSMUSG00000014609.
GeneID11448.
KEGGmmu:11448.
UCSCuc007jvo.1. mouse.

Organism-specific databases

CTD1145.
MGIMGI:87894. Chrne.

Phylogenomic databases

eggNOGNOG291810.
GeneTreeENSGT00750000117375.
HOGENOMHOG000006757.
HOVERGENHBG003756.
KOK04817.
OrthoDBEOG7H7925.
TreeFamTF315605.

Gene expression databases

BgeeP20782.
GenevestigatorP20782.

Family and domain databases

Gene3D1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio278760.
PROP20782.
SOURCESearch...

Entry information

Entry nameACHE_MOUSE
AccessionPrimary (citable) accession number: P20782
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot