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Protein

Acetylcholine receptor subunit epsilon

Gene

Chrne

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

GO - Biological processi

  • cation transmembrane transport Source: GOC
  • cation transport Source: GOC
  • regulation of membrane potential Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_305614. Highly sodium permeable acetylcholine nicotinic receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholine receptor subunit epsilon
Gene namesi
Name:Chrne
Synonyms:Acre
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:87894. Chrne.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 239219ExtracellularAdd
BLAST
Transmembranei240 – 26425HelicalSequence AnalysisAdd
BLAST
Topological domaini265 – 2728CytoplasmicSequence Analysis
Transmembranei273 – 29119HelicalSequence AnalysisAdd
BLAST
Topological domaini292 – 30615ExtracellularSequence AnalysisAdd
BLAST
Transmembranei307 – 32822HelicalSequence AnalysisAdd
BLAST
Topological domaini329 – 456128CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei457 – 48024HelicalSequence AnalysisAdd
BLAST
Topological domaini481 – 49313ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • integral component of membrane Source: UniProtKB-KW
  • postsynaptic membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Add
BLAST
Chaini21 – 493473Acetylcholine receptor subunit epsilonPRO_0000000330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi86 – 861N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi148 ↔ 162By similarity
Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP20782.
PRIDEiP20782.

Expressioni

Gene expression databases

BgeeiP20782.
ExpressionAtlasiP20782. baseline and differential.
GenevisibleiP20782. MM.

Interactioni

Subunit structurei

Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099616.

Structurei

3D structure databases

ProteinModelPortaliP20782.
SMRiP20782. Positions 22-337, 448-485.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG291810.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiP20782.
KOiK04817.
OrthoDBiEOG7H7925.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20782-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGALLGALL LLTLFGRSQG KNEELSLYHH LFDNYDPECR PVRRPEDTVT
60 70 80 90 100
ITLKVTLTNL ISLNEKEETL TTSVWIGIDW HDYRLNYSKD DFAGVGILRV
110 120 130 140 150
PSEHVWLPEI VLENNIDGQF GVAYDSNVLV YEGGYVSWLP PAIYRSTCAV
160 170 180 190 200
EVTYFPFDWQ NCSLIFRSQT YNAEEVEFIF AVDDDGNTIN KIDIDTAAFT
210 220 230 240 250
ENGEWAIDYC PGMIRRYEGG STEGPGETDV IYTLIIRRKP LFYVINIIVP
260 270 280 290 300
CVLISGLVLL AYFLPAQAGG QKCTVSINVL LAQTVFLFLI AQKIPETSLS
310 320 330 340 350
VPLLGRYLIF VMVVATLIVM NCVIVLNVSL RTPTTHATSP RLRQILLELL
360 370 380 390 400
PRLLGSSPPP EDPRTASPAR RASSVGILLR AEELILKKPR SELVFEGQRH
410 420 430 440 450
RHGTWTAALC QNLGAAAPEI RCCVDAVNFV AESTRDQEAT GEELSDWVRM
460 470 480 490
GKALDNVCFW AALVLFSVGS TLIFLGGYFN QVPDLPYPPC IQP
Length:493
Mass (Da):54,914
Last modified:February 1, 1991 - v1
Checksum:iF3E468644F3FB7DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04698 Genomic DNA. Translation: AAA37153.1.
X55718 mRNA. Translation: CAA39251.1.
CCDSiCCDS24956.1.
PIRiS13592. ACMSE.
RefSeqiNP_033733.1. NM_009603.1.
UniGeneiMm.4980.

Genome annotation databases

EnsembliENSMUST00000102556; ENSMUSP00000099616; ENSMUSG00000014609.
GeneIDi11448.
KEGGimmu:11448.
UCSCiuc007jvo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04698 Genomic DNA. Translation: AAA37153.1.
X55718 mRNA. Translation: CAA39251.1.
CCDSiCCDS24956.1.
PIRiS13592. ACMSE.
RefSeqiNP_033733.1. NM_009603.1.
UniGeneiMm.4980.

3D structure databases

ProteinModelPortaliP20782.
SMRiP20782. Positions 22-337, 448-485.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099616.

Chemistry

BindingDBiP20782.
ChEMBLiCHEMBL3137264.
GuidetoPHARMACOLOGYi477.

Proteomic databases

PaxDbiP20782.
PRIDEiP20782.

Protocols and materials databases

DNASUi11448.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102556; ENSMUSP00000099616; ENSMUSG00000014609.
GeneIDi11448.
KEGGimmu:11448.
UCSCiuc007jvo.1. mouse.

Organism-specific databases

CTDi1145.
MGIiMGI:87894. Chrne.

Phylogenomic databases

eggNOGiNOG291810.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiP20782.
KOiK04817.
OrthoDBiEOG7H7925.
TreeFamiTF315605.

Enzyme and pathway databases

ReactomeiREACT_305614. Highly sodium permeable acetylcholine nicotinic receptors.

Miscellaneous databases

NextBioi278760.
PROiP20782.
SOURCEiSearch...

Gene expression databases

BgeeiP20782.
ExpressionAtlasiP20782. baseline and differential.
GenevisibleiP20782. MM.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of the beta and epsilon subunit genes of mouse muscle acetylcholine receptor."
    Buonanno A., Mudd J., Merlie J.P.
    J. Biol. Chem. 264:7611-7616(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of the epsilon-subunit of the mouse muscle nicotinic acetylcholine receptor."
    Gardner P.D.
    Nucleic Acids Res. 18:6714-6714(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiACHE_MOUSE
AccessioniPrimary (citable) accession number: P20782
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 24, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.