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Protein

Glycine receptor subunit beta

Gene

Glrb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycine receptors are ligand-gated chloride channels. GLRB does not form ligand-gated ion channels by itself, but is part of heteromeric ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:15574743, PubMed:14551753). Heteropentameric channels composed of GLRB and GLRA1 are activated by lower glycine levels than homopentameric GLRA1. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei307 – 3071Important for obstruction of the ion pore in the closed conformationBy similarity

GO - Molecular functioni

  • drug binding Source: Ensembl
  • extracellular-glycine-gated chloride channel activity Source: Ensembl
  • extracellular-glycine-gated ion channel activity Source: UniProtKB
  • glycine binding Source: RGD

GO - Biological processi

  • acrosome reaction Source: Ensembl
  • adult walking behavior Source: Ensembl
  • chloride transmembrane transport Source: UniProtKB
  • gamma-aminobutyric acid receptor clustering Source: Ensembl
  • ion transport Source: UniProtKB
  • nervous system development Source: UniProtKB
  • neuropeptide signaling pathway Source: UniProtKB
  • protein heterooligomerization Source: RGD
  • regulation of membrane potential Source: Ensembl
  • righting reflex Source: Ensembl
  • startle response Source: UniProtKB
  • synaptic transmission Source: UniProtKB
  • synaptic transmission, glycinergic Source: RGD
  • visual perception Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-RNO-975298. Ligand-gated ion channel transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit beta
Alternative name(s):
Glycine receptor 58 kDa subunit
Gene namesi
Name:Glrb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2706. Glrb.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 268246ExtracellularBy similarityAdd
BLAST
Transmembranei269 – 29022Helical; Name=1By similarityAdd
BLAST
Topological domaini291 – 2955CytoplasmicBy similarity
Transmembranei296 – 31621Helical; Name=2By similarityAdd
BLAST
Topological domaini317 – 32711ExtracellularBy similarityAdd
BLAST
Transmembranei328 – 34821Helical; Name=3By similarityAdd
BLAST
Topological domaini349 – 474126CytoplasmicBy similarityAdd
BLAST
Transmembranei475 – 49521Helical; Name=4By similarityAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB-SubCell
  • dendrite Source: UniProtKB-SubCell
  • glycine-gated chloride channel complex Source: UniProtKB
  • integral component of membrane Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • perikaryon Source: UniProtKB-SubCell
  • postsynaptic membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3392921.
GuidetoPHARMACOLOGYi427.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Add
BLAST
Chaini23 – 496474Glycine receptor subunit betaPRO_0000000425Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence analysis
Disulfide bondi183 ↔ 197By similarity
Glycosylationi242 – 2421N-linked (GlcNAc...)Sequence analysis
Disulfide bondi243 ↔ 255By similarity
Modified residuei391 – 3911PhosphothreonineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP20781.

PTM databases

iPTMnetiP20781.

Expressioni

Tissue specificityi

Detected in spinal cord and brain stem (at protein level) (PubMed:22592841). Detected in spinal cord, cerebellum and brain cortex (PubMed:2163264).2 Publications

Gene expression databases

GenevisibleiP20781. RN.

Interactioni

Subunit structurei

Heteropentamer composed of GLRB and GLRA1 (PubMed:15574743, PubMed:8581315, PubMed:14551753). Heteropentamer composed of GLRB and GLRA2 (PubMed:8581315). Heteropentamer composed of GLRB and GLRA3 (PubMed:8581315). Heteropentamer composed of two GLRA1 and three GLRB subunits. Heteropentamer composed of three GLRA1 and two GLRB subunits (By similarity). Interacts with GPHN (PubMed:8581315, PubMed:15201864, PubMed:16511563).By similarity4 Publications

Protein-protein interaction databases

BioGridi247490. 6 interactions.
IntActiP20781. 1 interaction.
STRINGi10116.ENSRNOP00000014170.

Structurei

Secondary structure

1
496
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi429 – 4313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T3EX-ray3.25P378-426[»]
2FTSX-ray2.41P420-432[»]
4PD1X-ray1.98C419-433[»]
ProteinModelPortaliP20781.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20781.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni247 – 2537Strychnine-bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3644. Eukaryota.
ENOG410XP43. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiP20781.
KOiK05196.
OMAiPRLKLPN.
OrthoDBiEOG712TVZ.
PhylomeDBiP20781.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR008060. Glycine_rcpt_B.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PTHR18945:SF29. PTHR18945:SF29. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01677. GLYRBETA.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20781-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFSLAVSFF ILMSLLFEDA CSKEKSSKKG KGKKKQYLCP SQQSAEDLAR
60 70 80 90 100
VPPNSTSNIL NRLLVSYDPR IRPNFKGIPV DVVVNIFINS FGSIQETTMD
110 120 130 140 150
YRVNIFLRQK WNDPRLKLPS DFRGSDALTV DPTMYKCLWK PDLFFANEKS
160 170 180 190 200
ANFHDVTQEN ILLFIFRDGD VLVSMRLSIT LSCPLDLTLF PMDTQRCKMQ
210 220 230 240 250
LESFGYTTDD LRFIWQSGDP VQLEKIALPQ FDIKKEDIEY GNCTKYYKGT
260 270 280 290 300
GYYTCVEVIF TLRRQVGFYM MGVYAPTLLI VVLSWLSFWI NPDASAARVP
310 320 330 340 350
LGIFSVLSLA SECTTLAAEL PKVSYVKALD VWLIACLLFG FASLVEYAVV
360 370 380 390 400
QVMLNNPKRV EAEKARIAKA EQADGKGGNA AKKNTVNGTG TPVHISTLQV
410 420 430 440 450
GETRCKKVCT SKSDLRSNDF SIVGSLPRDF ELSNYDCYGK PIEVNNGLGK
460 470 480 490
PQAKNKKPPP AKPVIPTAAK RIDLYARALF PFCFLFFNVI YWSIYL
Length:496
Mass (Da):55,927
Last modified:February 1, 1991 - v1
Checksum:iF77CE96105EF8ACF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ310839 mRNA. Translation: CAC35983.1.
PIRiJH0165.
RefSeqiNP_445748.1. NM_053296.1.
XP_006232568.1. XM_006232506.2.
UniGeneiRn.94903.

Genome annotation databases

EnsembliENSRNOT00000014170; ENSRNOP00000014170; ENSRNOG00000010199.
GeneIDi25456.
KEGGirno:25456.
UCSCiRGD:2706. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ310839 mRNA. Translation: CAC35983.1.
PIRiJH0165.
RefSeqiNP_445748.1. NM_053296.1.
XP_006232568.1. XM_006232506.2.
UniGeneiRn.94903.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T3EX-ray3.25P378-426[»]
2FTSX-ray2.41P420-432[»]
4PD1X-ray1.98C419-433[»]
ProteinModelPortaliP20781.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247490. 6 interactions.
IntActiP20781. 1 interaction.
STRINGi10116.ENSRNOP00000014170.

Chemistry

ChEMBLiCHEMBL3392921.
GuidetoPHARMACOLOGYi427.

PTM databases

iPTMnetiP20781.

Proteomic databases

PaxDbiP20781.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014170; ENSRNOP00000014170; ENSRNOG00000010199.
GeneIDi25456.
KEGGirno:25456.
UCSCiRGD:2706. rat.

Organism-specific databases

CTDi2743.
RGDi2706. Glrb.

Phylogenomic databases

eggNOGiKOG3644. Eukaryota.
ENOG410XP43. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiP20781.
KOiK05196.
OMAiPRLKLPN.
OrthoDBiEOG712TVZ.
PhylomeDBiP20781.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiR-RNO-975298. Ligand-gated ion channel transport.

Miscellaneous databases

EvolutionaryTraceiP20781.
PROiP20781.

Gene expression databases

GenevisibleiP20781. RN.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR008060. Glycine_rcpt_B.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PTHR18945:SF29. PTHR18945:SF29. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01677. GLYRBETA.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the 58 kd beta subunit of the inhibitory glycine receptor."
    Grenningloh G., Pribilla I., Prior P., Multhaup G., Beyreuther K., Taleb O., Betz H.
    Neuron 4:963-970(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY.
  2. "Single-channel behavior of heteromeric alpha1beta glycine receptors: an attempt to detect a conformational change before the channel opens."
    Burzomato V., Beato M., Groot Kormelink P.J., Colquhoun D., Sivilotti L.G.
    J. Neurosci. 24:10924-10940(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH GLRA1, SUBCELLULAR LOCATION, SUBUNIT.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "Targeting of glycine receptor subunits to gephyrin-rich domains in transfected human embryonic kidney cells."
    Kirsch J., Kuhse J., Betz H.
    Mol. Cell. Neurosci. 6:450-461(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH GPHN; GLRA1; GLRA2 AND GLRA3.
  4. "Kinetic analysis of recombinant mammalian alpha(1) and alpha(1)beta glycine receptor channels."
    Mohammadi B., Krampfl K., Cetinkaya C., Moschref H., Grosskreutz J., Dengler R., Bufler J.
    Eur. Biophys. J. 32:529-536(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GLRA1, FUNCTION, SUBCELLULAR LOCATION.
  5. "Distribution of the glycine receptor beta-subunit in the mouse CNS as revealed by a novel monoclonal antibody."
    Weltzien F., Puller C., O'Sullivan G.A., Paarmann I., Betz H.
    J. Comp. Neurol. 520:3962-3981(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  6. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-391, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF 378-426 IN COMPLEX WITH GPHN.
  8. "Deciphering the structural framework of glycine receptor anchoring by gephyrin."
    Kim E.Y., Schrader N., Smolinsky B., Bedet C., Vannier C., Schwarz G., Schindelin H.
    EMBO J. 25:1385-1395(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.41 ANGSTROMS) OF 420-432 IN COMPLEX WITH GPHN.

Entry informationi

Entry nameiGLRB_RAT
AccessioniPrimary (citable) accession number: P20781
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 8, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.