Glycine receptor subunit beta precursor

Contribute

Send feedback

Read comments (?) or add your own

P20781 (GLRB_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glycine receptor subunit beta

Alternative name(s):
Glycine receptor 58 kDa subunit

Gene names

Name:

Glrb

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
Subunit structure	Pentamer composed of alpha and beta subunits. Interacts with GPHN.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRB sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ionic channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	nervous system development Inferred from sequence or structural similarity. Source: UniProtKB neuropeptide signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB protein heterooligomerization Inferred from direct assay Ref.2. Source: RGD startle response Inferred from sequence or structural similarity. Source: UniProtKB synaptic transmission, glycinergic Inferred from direct assay Ref.2. Source: RGD
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Traceable author statement. Source: RGD
Molecular function	glycine binding Inferred from direct assay Ref.2. Source: RGD protein binding Inferred from physical interaction. Source: RGD receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

Chain

23 – 496

474

Glycine receptor subunit beta

PRO_0000000425

Regions

Topological domain

23 – 265

243

Extracellular Probable

Transmembrane

266 – 290

Helical; Probable

Transmembrane

299 – 316

Helical; Probable

Transmembrane

331 – 354

Helical; Probable

Topological domain

355 – 477

123

Cytoplasmic Probable

Transmembrane

478 – 495

Helical; Probable

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

242

N-linked (GlcNAc...) Potential

Disulfide bond

183 ↔ 197

By similarity

Secondary structure

496

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P20781 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: F77CE96105EF8ACF
Show »

FASTA

496

55,927

        10         20         30         40         50         60 
MKFSLAVSFF ILMSLLFEDA CSKEKSSKKG KGKKKQYLCP SQQSAEDLAR VPPNSTSNIL 

        70         80         90        100        110        120 
NRLLVSYDPR IRPNFKGIPV DVVVNIFINS FGSIQETTMD YRVNIFLRQK WNDPRLKLPS 

       130        140        150        160        170        180 
DFRGSDALTV DPTMYKCLWK PDLFFANEKS ANFHDVTQEN ILLFIFRDGD VLVSMRLSIT 

       190        200        210        220        230        240 
LSCPLDLTLF PMDTQRCKMQ LESFGYTTDD LRFIWQSGDP VQLEKIALPQ FDIKKEDIEY 

       250        260        270        280        290        300 
GNCTKYYKGT GYYTCVEVIF TLRRQVGFYM MGVYAPTLLI VVLSWLSFWI NPDASAARVP 

       310        320        330        340        350        360 
LGIFSVLSLA SECTTLAAEL PKVSYVKALD VWLIACLLFG FASLVEYAVV QVMLNNPKRV 

       370        380        390        400        410        420 
EAEKARIAKA EQADGKGGNA AKKNTVNGTG TPVHISTLQV GETRCKKVCT SKSDLRSNDF 

       430        440        450        460        470        480 
SIVGSLPRDF ELSNYDCYGK PIEVNNGLGK PQAKNKKPPP AKPVIPTAAK RIDLYARALF 

       490 
PFCFLFFNVI YWSIYL

« Hide

References

[1]	"Cloning and expression of the 58 kd beta subunit of the inhibitory glycine receptor." Grenningloh G., Pribilla I., Prior P., Multhaup G., Beyreuther K., Taleb O., Betz H. Neuron 4:963-970(1990) [PubMed: 2163264] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Single-channel behavior of heteromeric alpha1beta glycine receptors: an attempt to detect a conformational change before the channel opens." Burzomato V., Beato M., Groot Kormelink P.J., Colquhoun D., Sivilotti L.G. J. Neurosci. 24:10924-10940(2004) [PubMed: 15574743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[3]	"Structural basis of dynamic glycine receptor clustering by gephyrin." Sola M., Bavro V.N., Timmins J., Franz T., Ricard-Blum S., Schoehn G., Ruigrok R.W.H., Paarmann I., Saiyed T., O'Sullivan G.A., Schmitt B., Betz H., Weissenhorn W. EMBO J. 23:2510-2519(2004) [PubMed: 15201864] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF 378-426 IN COMPLEX WITH GPHN.
[4]	"Deciphering the structural framework of glycine receptor anchoring by gephyrin." Kim E.Y., Schrader N., Smolinsky B., Bedet C., Vannier C., Schwarz G., Schindelin H. EMBO J. 25:1385-1395(2006) [PubMed: 16511563] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.41 ANGSTROMS) OF 420-432 IN COMPLEX WITH GPHN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ310839 mRNA. Translation: CAC35983.1.

IPI

IPI00202491.

PIR

JH0165.

RefSeq

NP_445748.1. NM_053296.1.

UniGene

Rn.94903.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1T3E	X-ray	3.25	P	378-426	[»]
2FTS	X-ray	2.41	P	420-432	[»]

ProteinModelPortal

P20781.

ModBase

Search...

Protein-protein interaction databases

IntAct

P20781. 1 interaction.

STRING

P20781.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000014170; ENSRNOP00000014170; ENSRNOG00000010199.

GeneID

25456.

KEGG

rno:25456.

Organism-specific databases

CTD

2743.

RGD

2706. Glrb.

Phylogenomic databases

eggNOG

roNOG09626.

GeneTree

ENSGT00550000074453.

HOVERGEN

HBG051707.

InParanoid

P20781.

OMA

PTMFKCL.

OrthoDB

EOG4HMJ97.

PhylomeDB

P20781.

Gene expression databases

ArrayExpress

P20781.

Genevestigator

P20781.

GermOnline

ENSRNOG00000010199. Rattus norvegicus.

Family and domain databases

InterPro

IPR008060. Glycine_rcpt_B.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]

Gene3D

G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.

K05196.

PANTHER

PTHR18945:SF29. Glycine_rcpt_B. 1 hit.
PTHR18945. Neur_channel. 1 hit.

Pfam

PF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]

PRINTS

PR01677. GLYRBETA.
PR00252. NRIONCHANNEL.

SUPFAM

SSF90112. Neu_channel_TM. 1 hit.
SSF63712. Neur_chan_LBD. 1 hit.

TIGRFAMs

TIGR00860. LIC. 1 hit.

PROSITE

PS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

606717.

Entry information

Entry name

GLRB_RAT

Accession

Primary (citable) accession number: P20781

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	November 16, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents