Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycine receptor subunit beta

Gene

Glrb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycine receptors are ligand-gated chloride channels. GLRB does not form ligand-gated ion channels by itself, but is part of heteromeric ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:15574743, PubMed:14551753). Heteropentameric channels composed of GLRB and GLRA1 are activated by lower glycine levels than homopentameric GLRA1. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei307Important for obstruction of the ion pore in the closed conformationBy similarity1

GO - Molecular functioni

GO - Biological processi

  • acrosome reaction Source: Ensembl
  • adult walking behavior Source: Ensembl
  • chemical synaptic transmission Source: UniProtKB
  • chloride transmembrane transport Source: UniProtKB
  • gamma-aminobutyric acid receptor clustering Source: Ensembl
  • ion transport Source: UniProtKB
  • nervous system development Source: UniProtKB
  • neuropeptide signaling pathway Source: UniProtKB
  • protein heterooligomerization Source: RGD
  • response to amino acid Source: GO_Central
  • righting reflex Source: Ensembl
  • startle response Source: UniProtKB
  • synaptic transmission, glycinergic Source: RGD
  • visual perception Source: Ensembl

Keywordsi

Molecular functionChloride channel, Ion channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport
LigandChloride

Enzyme and pathway databases

ReactomeiR-RNO-112314 Neurotransmitter receptors and postsynaptic signal transmission

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit beta
Alternative name(s):
Glycine receptor 58 kDa subunit
Gene namesi
Name:Glrb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2706 Glrb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 268ExtracellularBy similarityAdd BLAST246
Transmembranei269 – 290Helical; Name=1By similarityAdd BLAST22
Topological domaini291 – 295CytoplasmicBy similarity5
Transmembranei296 – 316Helical; Name=2By similarityAdd BLAST21
Topological domaini317 – 327ExtracellularBy similarityAdd BLAST11
Transmembranei328 – 348Helical; Name=3By similarityAdd BLAST21
Topological domaini349 – 474CytoplasmicBy similarityAdd BLAST126
Transmembranei475 – 495Helical; Name=4By similarityAdd BLAST21

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3392921
GuidetoPHARMACOLOGYi427

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000000042523 – 496Glycine receptor subunit betaAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi54N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi183 ↔ 197By similarity
Glycosylationi242N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi243 ↔ 255By similarity
Modified residuei391PhosphothreonineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP20781
PRIDEiP20781

PTM databases

iPTMnetiP20781
PhosphoSitePlusiP20781

Expressioni

Tissue specificityi

Detected in spinal cord and brain stem (at protein level) (PubMed:22592841). Detected in spinal cord, cerebellum and brain cortex (PubMed:2163264).2 Publications

Gene expression databases

BgeeiENSRNOG00000010199
GenevisibleiP20781 RN

Interactioni

Subunit structurei

Heteropentamer composed of GLRB and GLRA1 (PubMed:15574743, PubMed:8581315, PubMed:14551753). Heteropentamer composed of GLRB and GLRA2 (PubMed:8581315). Heteropentamer composed of GLRB and GLRA3 (PubMed:8581315). Heteropentamer composed of two GLRA1 and three GLRB subunits. Heteropentamer composed of three GLRA1 and two GLRB subunits (By similarity). Interacts with GPHN (PubMed:8581315, PubMed:15201864, PubMed:16511563).By similarity4 Publications

Protein-protein interaction databases

BioGridi247490, 6 interactors
IntActiP20781, 1 interactor
STRINGi10116.ENSRNOP00000014170

Structurei

Secondary structure

1496
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi429 – 431Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T3EX-ray3.25P378-426[»]
2FTSX-ray2.41P420-432[»]
4PD1X-ray1.98C419-433[»]
ProteinModelPortaliP20781
SMRiP20781
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20781

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni247 – 253Strychnine-bindingBy similarity7

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3644 Eukaryota
ENOG410XP43 LUCA
GeneTreeiENSGT00760000118821
HOGENOMiHOG000231336
HOVERGENiHBG051707
InParanoidiP20781
KOiK05196
OMAiPRLKLPN
OrthoDBiEOG091G0805
PhylomeDBiP20781
TreeFamiTF315453

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR008060 Glycine_rcpt_B
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERiPTHR18945 PTHR18945, 1 hit
PTHR18945:SF29 PTHR18945:SF29, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR01677 GLYRBETA
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20781-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFSLAVSFF ILMSLLFEDA CSKEKSSKKG KGKKKQYLCP SQQSAEDLAR
60 70 80 90 100
VPPNSTSNIL NRLLVSYDPR IRPNFKGIPV DVVVNIFINS FGSIQETTMD
110 120 130 140 150
YRVNIFLRQK WNDPRLKLPS DFRGSDALTV DPTMYKCLWK PDLFFANEKS
160 170 180 190 200
ANFHDVTQEN ILLFIFRDGD VLVSMRLSIT LSCPLDLTLF PMDTQRCKMQ
210 220 230 240 250
LESFGYTTDD LRFIWQSGDP VQLEKIALPQ FDIKKEDIEY GNCTKYYKGT
260 270 280 290 300
GYYTCVEVIF TLRRQVGFYM MGVYAPTLLI VVLSWLSFWI NPDASAARVP
310 320 330 340 350
LGIFSVLSLA SECTTLAAEL PKVSYVKALD VWLIACLLFG FASLVEYAVV
360 370 380 390 400
QVMLNNPKRV EAEKARIAKA EQADGKGGNA AKKNTVNGTG TPVHISTLQV
410 420 430 440 450
GETRCKKVCT SKSDLRSNDF SIVGSLPRDF ELSNYDCYGK PIEVNNGLGK
460 470 480 490
PQAKNKKPPP AKPVIPTAAK RIDLYARALF PFCFLFFNVI YWSIYL
Length:496
Mass (Da):55,927
Last modified:February 1, 1991 - v1
Checksum:iF77CE96105EF8ACF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ310839 mRNA Translation: CAC35983.1
PIRiJH0165
RefSeqiNP_445748.1, NM_053296.1
XP_006232568.1, XM_006232506.2
UniGeneiRn.94903

Genome annotation databases

EnsembliENSRNOT00000014170; ENSRNOP00000014170; ENSRNOG00000010199
GeneIDi25456
KEGGirno:25456
UCSCiRGD:2706 rat

Similar proteinsi

Entry informationi

Entry nameiGLRB_RAT
AccessioniPrimary (citable) accession number: P20781
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health