P20774 (MIME_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mimecan

Alternative name(s):
Osteoglycin
Osteoinductive factor
Short name=OIF

Gene names

Name:	OGN
Synonyms:	OIF, SLRR3A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Bone.
Post-translational modification	N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Sequence similarities	Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily. Contains 7 LRR (leucine-rich) repeats.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Leucine-rich repeat Repeat Signal
Molecular function	Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Traceable author statement. Source: Reactome keratan sulfate biosynthetic process Traceable author statement. Source: Reactome keratan sulfate catabolic process Traceable author statement. Source: Reactome negative regulation of smooth muscle cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Golgi lumen Traceable author statement. Source: Reactome extracellular space Inferred from direct assay PubMed 20551380. Source: BHF-UCL lysosomal lumen Traceable author statement. Source: Reactome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CRK	P46108	2	EBI-1753690,EBI-886

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.10
	Chain	21 – 298	278	Mimecan		PRO_0000032760
Regions
	Repeat	112 – 131	20	LRR 1
	Repeat	132 – 155	24	LRR 2
	Repeat	156 – 179	24	LRR 3
	Repeat	180 – 199	20	LRR 4
	Repeat	200 – 225	26	LRR 5
	Repeat	226 – 246	21	LRR 6
	Repeat	247 – 277	31	LRR 7
	Compositional bias	94 – 106	13	Cys-rich
Amino acid modifications
	Glycosylation	80	1	O-linked (GalNAc...) Ref.13
	Glycosylation	214	1	N-linked (GlcNAc...) Potential
	Glycosylation	258	1	N-linked (GlcNAc...) Ref.12
	Disulfide bond	255 ↔ 288		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20774 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: F4590785C3849F83	FASTA	298	33,922
	10 20 30 40 50 60 MKTLQSTLLL LLLVPLIKPA PPTQQDSRII YDYGTDNFEE SIFSQDYEDK YLDGKNIKEK 70 80 90 100 110 120 ETVIIPNEKS LQLQKDEAIT PLPPKKENDE MPTCLLCVCL SGSVYCEEVD IDAVPPLPKE 130 140 150 160 170 180 SAYLYARFNK IKKLTAKDFA DIPNLRRLDF TGNLIEDIED GTFSKLSLLE ELSLAENQLL 190 200 210 220 230 240 KLPVLPPKLT LFNAKYNKIK SRGIKANAFK KLNNLTFLYL DHNALESVPL NLPESLRVIH 250 260 270 280 290 LQFNNIASIT DDTFCKANDT SYIRDRIEEI RLEGNPIVLG KHPNSFICLK RLPIGSYF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel bone-forming compound: osteoinductive factor." Madisen L., Neubauer M., Plowman G., Rosen D.M., Segarini P.R., Dasch J.R., Thompson A.Y., Ziman J., Bentz H., Purchio A.F. DNA Cell Biol. 9:303-309(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Grover J., Rodriguez E., Roughley P.J. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Mutations in KERA, encoding keratocan, cause cornea plana." Pellegata N.S., Dieguez-Lucena J.L., Joensuu T., Lau S., Montgomery K.T., Krahe R., Kivelae T., Kucherlapati R., Forsius H., de la Chapelle A. Nat. Genet. 25:91-95(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[5]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[6]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[9]	"The bovine mimecan gene. Molecular cloning and characterization of two major RNA transcripts generated by alternative use of two splice acceptor sites in the third exon." Tasheva E.S., Funderburgh M.L., McReynolds J., Funderburgh J.L., Conrad G.W. J. Biol. Chem. 274:18693-18701(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54.
[10]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-35.
[11]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 156-298. Tissue: Uterus.
[12]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-258, MASS SPECTROMETRY. Tissue: Liver.
[13]	"Enrichment of glycopeptides for glycan structure and attachment site identification." Nilsson J., Rueetschi U., Halim A., Hesse C., Carlsohn E., Brinkmalm G., Larson G. Nat. Methods 6:809-811(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-80, STRUCTURE OF CARBOHYDRATES, MASS SPECTROMETRY. Tissue: Cerebrospinal fluid.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF192483 , AF192478, AF192479, AF192480, AF192481, AF192482 Genomic DNA. Translation: AAF19364.1. AF202167 mRNA. Translation: AAF69109.1. AF100758 mRNA. Translation: AAD43022.1. AL110267 mRNA. Translation: CAB53706.1. CR533516 mRNA. Translation: CAG38547.1. AL137848 Genomic DNA. Translation: CAI16694.1. BC037273 mRNA. Translation: AAH37273.1. BC095443 mRNA. Translation: AAH95443.1. AF112465 Genomic DNA. Translation: AAD40453.1. AL133114 mRNA. Translation: CAB61417.2.
IPI	IPI00025465.
PIR	B35272.
RefSeq	NP_054776.1. NM_014057.3. NP_148935.1. NM_033014.2.
UniGene	Hs.109439.
3D structure databases
ProteinModelPortal	P20774.
ModBase	Search...
Protein-protein interaction databases
IntAct	P20774. 3 interactions.
MINT	MINT-4529844.
STRING	9606.ENSP00000262551.
PTM databases
PhosphoSite	P20774.
Polymorphism databases
DMDM	129078.
2D gel databases
REPRODUCTION-2DPAGE	IPI00025465.
Proteomic databases
PaxDb	P20774.
PeptideAtlas	P20774.
PRIDE	P20774.
Protocols and materials databases
DNASU	4969.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000262551; ENSP00000262551; ENSG00000106809. ENST00000375561; ENSP00000364711; ENSG00000106809.
GeneID	4969.
KEGG	hsa:4969.
UCSC	uc004asa.3. human.
Organism-specific databases
CTD	4969.
GeneCards	GC09M095146.
HGNC	HGNC:8126. OGN.
HPA	HPA013132.
MIM	602383. gene.
neXtProt	NX_P20774.
PharmGKB	PA31913.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG318805.
HOVERGEN	HBG006850.
InParanoid	P20774.
KO	K08126.
OMA	DTRYIRD.
OrthoDB	EOG454906.
PhylomeDB	P20774.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_116125. Disease.
Gene expression databases
ArrayExpress	P20774.
Bgee	P20774.
CleanEx	HS_OGN.
Genevestigator	P20774.
GermOnline	ENSG00000106809. Homo sapiens.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. IPR000372. LRR-contain_N. IPR027211. Mimecan. [Graphical view]
PANTHER	PTHR24371:SF5. PTHR24371:SF5. 1 hit.
SMART	SM00013. LRRNT. 1 hit. [Graphical view]
PROSITE	PS51450. LRR. 4 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	4969.
NextBio	19134.
PMAP-CutDB	P20774.
SOURCE	Search...

Entry information

Entry name

MIME_HUMAN

Accession

Primary (citable) accession number: P20774
Secondary accession number(s): Q6FIB0, Q9UF90, Q9UNK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 1, 2013
This is version 132 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.