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Protein

Bifunctional purine biosynthetic protein ADE1

Gene

ade1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable phosphoribosylformylglycinamidine synthase (ade3)
  2. Bifunctional purine biosynthetic protein ADE1 (ade1)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (ade4)
  2. Bifunctional purine biosynthetic protein ADE1 (ade1)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi291ManganeseBy similarity1
Metal bindingi293ManganeseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi141 – 202ATPBy similarityAdd BLAST62

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • phosphoribosylamine-glycine ligase activity Source: PomBase
  • phosphoribosylformylglycinamidine cyclo-ligase activity Source: PomBase

GO - Biological processi

  • 'de novo' IMP biosynthetic process Source: PomBase
  • purine nucleobase biosynthetic process Source: PomBase

Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processPurine biosynthesis
LigandATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00125
UPA00074; UER00129

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthetic protein ADE1
Including the following 2 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:ade1
ORF Names:SPBC405.01, SPBC4C3.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC405.01
PomBaseiSPBC405.01 ade1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749391 – 788Bifunctional purine biosynthetic protein ADE1Add BLAST788

Proteomic databases

MaxQBiP20772
PaxDbiP20772
PRIDEiP20772

PTM databases

iPTMnetiP20772

Interactioni

Protein-protein interaction databases

BioGridi277549, 1 interactor
STRINGi4896.SPBC405.01.1

Structurei

3D structure databases

ProteinModelPortaliP20772
SMRiP20772
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini115 – 321ATP-graspAdd BLAST207

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 430GARSAdd BLAST430
Regioni440 – 750AIRSAdd BLAST311

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the C-terminal section; belongs to the AIR synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000030315
InParanoidiP20772
KOiK11788
OMAiLLERHNC
OrthoDBiEOG092C3N9W
PhylomeDBiP20772

Family and domain databases

CDDicd02196 PurM, 1 hit
Gene3Di3.30.1330.10, 1 hit
3.30.1490.20, 1 hit
3.90.600.10, 1 hit
3.90.650.10, 1 hit
HAMAPiMF_00741 AIRS, 1 hit
MF_00138 GARS, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
IPR004733 PurM_cligase
IPR011054 Rudment_hybrid_motif
PANTHERiPTHR10520 PTHR10520, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 1 hit
PF02769 AIRS_C, 1 hit
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
SSF55326 SSF55326, 1 hit
SSF56042 SSF56042, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
TIGR00878 purM, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit

Sequencei

Sequence statusi: Complete.

P20772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPIIALLIG NGGREHTIAW KLCESPLISK VYVAPGNGGT ASNGAESKME
60 70 80 90 100
NVNIGVCDFE QLVKFALDKD VNLVIPGPEL PLVEGIEGHF RRVGIPCFGP
110 120 130 140 150
SALAARMEGS KVFSKDFMHR NNIPTAVYKS FSNYDHAKSF LDTCTFDVVI
160 170 180 190 200
KADGLAAGKG VIIPKTKKEA FEALESIMLN EEFGSAGKNV VIEELLEGEE
210 220 230 240 250
LSILTFSDGY TCRSLPPAQD HKRAFDGDKG PNTGGMGCYA PTPVASPKLL
260 270 280 290 300
ETVQSTIIQP TIDGMRHEGY PLVGILFTGL MLTPSGPRVL EYNVRFGDPE
310 320 330 340 350
TQAVLPLLES DLAEIILACV NHRLDAIDIV ISRKFSCAVV CVAGGYPGPY
360 370 380 390 400
NKGDIITFDA LKDKNTRIFH AGTSIRDGNV VTNGGRVLAV EATGDSVEAA
410 420 430 440 450
VRLAYEGVKT VHFDKMFYRK DIAHHALNPK RKTREILTYE NSGVSVDNGN
460 470 480 490 500
EFVQRIKDLV KSTRRPGADA DIGGFGGIFD LKQAGWNDPL LVSATDGVGS
510 520 530 540 550
KLLIALSLNK HDTVGIDLVA MNVNDLVVQG AEPLIFLDYF ATGSLDLKVS
560 570 580 590 600
TSFVEGVVKG CKQAGCALVG GETSEMPGLY HDGHYDANGT SVGAVSRDDI
610 620 630 640 650
LPKPESFSKG DILLGLASDG VHSNGYSLVR KIVEYSDLEY TSVCPWDKNV
660 670 680 690 700
RLGDSLLIPT RIYVKPLLHV IRKNIVKGMA HITGGGLVEN VPRMLPSHLN
710 720 730 740 750
AIIDVDTWEV PEVFKWLKDA GNVPISDMAR TFNMGIGMVV AVASEDAEET
760 770 780
MKELTSVGET VYRIGQLVDK ESSSERCHLV NLNKWETF
Length:788
Mass (Da):85,231
Last modified:February 1, 1991 - v1
Checksum:i0FDE64EEA5F9095D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06601 Genomic DNA Translation: CAA29820.1
CU329671 Genomic DNA Translation: CAA16823.1
PIRiS00652
RefSeqiNP_596304.1, NM_001022225.2

Genome annotation databases

EnsemblFungiiSPBC405.01.1; SPBC405.01.1:pep; SPBC405.01
KEGGispo:SPBC405.01

Similar proteinsi

Entry informationi

Entry nameiPUR2_SCHPO
AccessioniPrimary (citable) accession number: P20772
Secondary accession number(s): Q9UUM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health