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P20772

- PUR2_SCHPO

UniProt

P20772 - PUR2_SCHPO

Protein

Bifunctional purine biosynthetic protein ADE1

Gene

ade1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
    ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi291 – 2911ManganeseBy similarity
    Metal bindingi293 – 2931ManganeseBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi141 – 20262ATPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. phosphoribosylamine-glycine ligase activity Source: PomBase
    4. phosphoribosylformylglycinamidine cyclo-ligase activity Source: PomBase

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: PomBase
    2. purine nucleobase biosynthetic process Source: PomBase

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_207552. Purine ribonucleoside monophosphate biosynthesis.
    UniPathwayiUPA00074; UER00125.
    UPA00074; UER00129.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional purine biosynthetic protein ADE1
    Including the following 2 domains:
    Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name:
    GARS
    Phosphoribosylglycinamide synthetase
    Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
    Alternative name(s):
    AIR synthase
    Short name:
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    Gene namesi
    Name:ade1
    ORF Names:SPBC405.01, SPBC4C3.02c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC405.01.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 788788Bifunctional purine biosynthetic protein ADE1PRO_0000074939Add
    BLAST

    Proteomic databases

    MaxQBiP20772.
    PaxDbiP20772.

    Interactioni

    Protein-protein interaction databases

    BioGridi277549. 3 interactions.
    MINTiMINT-4687567.
    STRINGi4896.SPBC405.01-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP20772.
    SMRiP20772. Positions 477-772.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini115 – 321207ATP-graspAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 430430GARSAdd
    BLAST
    Regioni440 – 750311AIRSAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the GARS family.Curated
    In the C-terminal section; belongs to the AIR synthase family.Curated
    Contains 1 ATP-grasp domain.Curated

    Phylogenomic databases

    eggNOGiCOG0151.
    HOGENOMiHOG000030315.
    KOiK11788.
    OMAiGMLFTGF.
    OrthoDBiEOG7Q8CXX.
    PhylomeDBiP20772.

    Family and domain databases

    Gene3Di3.30.1330.10. 1 hit.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    3.90.650.10. 1 hit.
    HAMAPiMF_00138. GARS.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 1 hit.
    PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    SSF55326. SSF55326. 1 hit.
    SSF56042. SSF56042. 1 hit.
    TIGRFAMsiTIGR00877. purD. 1 hit.
    TIGR00878. purM. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20772-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPIIALLIG NGGREHTIAW KLCESPLISK VYVAPGNGGT ASNGAESKME    50
    NVNIGVCDFE QLVKFALDKD VNLVIPGPEL PLVEGIEGHF RRVGIPCFGP 100
    SALAARMEGS KVFSKDFMHR NNIPTAVYKS FSNYDHAKSF LDTCTFDVVI 150
    KADGLAAGKG VIIPKTKKEA FEALESIMLN EEFGSAGKNV VIEELLEGEE 200
    LSILTFSDGY TCRSLPPAQD HKRAFDGDKG PNTGGMGCYA PTPVASPKLL 250
    ETVQSTIIQP TIDGMRHEGY PLVGILFTGL MLTPSGPRVL EYNVRFGDPE 300
    TQAVLPLLES DLAEIILACV NHRLDAIDIV ISRKFSCAVV CVAGGYPGPY 350
    NKGDIITFDA LKDKNTRIFH AGTSIRDGNV VTNGGRVLAV EATGDSVEAA 400
    VRLAYEGVKT VHFDKMFYRK DIAHHALNPK RKTREILTYE NSGVSVDNGN 450
    EFVQRIKDLV KSTRRPGADA DIGGFGGIFD LKQAGWNDPL LVSATDGVGS 500
    KLLIALSLNK HDTVGIDLVA MNVNDLVVQG AEPLIFLDYF ATGSLDLKVS 550
    TSFVEGVVKG CKQAGCALVG GETSEMPGLY HDGHYDANGT SVGAVSRDDI 600
    LPKPESFSKG DILLGLASDG VHSNGYSLVR KIVEYSDLEY TSVCPWDKNV 650
    RLGDSLLIPT RIYVKPLLHV IRKNIVKGMA HITGGGLVEN VPRMLPSHLN 700
    AIIDVDTWEV PEVFKWLKDA GNVPISDMAR TFNMGIGMVV AVASEDAEET 750
    MKELTSVGET VYRIGQLVDK ESSSERCHLV NLNKWETF 788
    Length:788
    Mass (Da):85,231
    Last modified:February 1, 1991 - v1
    Checksum:i0FDE64EEA5F9095D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06601 Genomic DNA. Translation: CAA29820.1.
    CU329671 Genomic DNA. Translation: CAA16823.1.
    PIRiS00652.
    RefSeqiNP_596304.1. NM_001022225.2.

    Genome annotation databases

    EnsemblFungiiSPBC405.01.1; SPBC405.01.1:pep; SPBC405.01.
    GeneIDi2541034.
    KEGGispo:SPBC405.01.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06601 Genomic DNA. Translation: CAA29820.1 .
    CU329671 Genomic DNA. Translation: CAA16823.1 .
    PIRi S00652.
    RefSeqi NP_596304.1. NM_001022225.2.

    3D structure databases

    ProteinModelPortali P20772.
    SMRi P20772. Positions 477-772.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277549. 3 interactions.
    MINTi MINT-4687567.
    STRINGi 4896.SPBC405.01-1.

    Proteomic databases

    MaxQBi P20772.
    PaxDbi P20772.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC405.01.1 ; SPBC405.01.1:pep ; SPBC405.01 .
    GeneIDi 2541034.
    KEGGi spo:SPBC405.01.

    Organism-specific databases

    PomBasei SPBC405.01.

    Phylogenomic databases

    eggNOGi COG0151.
    HOGENOMi HOG000030315.
    KOi K11788.
    OMAi GMLFTGF.
    OrthoDBi EOG7Q8CXX.
    PhylomeDBi P20772.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00125 .
    UPA00074 ; UER00129 .
    Reactomei REACT_207552. Purine ribonucleoside monophosphate biosynthesis.

    Miscellaneous databases

    NextBioi 20802148.
    PROi P20772.

    Family and domain databases

    Gene3Di 3.30.1330.10. 1 hit.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    3.90.650.10. 1 hit.
    HAMAPi MF_00138. GARS.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view ]
    Pfami PF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 1 hit.
    PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    SSF55326. SSF55326. 1 hit.
    SSF56042. SSF56042. 1 hit.
    TIGRFAMsi TIGR00877. purD. 1 hit.
    TIGR00878. purM. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the bifunctional ade1 gene in the purine biosynthetic pathway of the fission yeast Schizosaccharomyces pombe."
      McKenzie R., Schuchert P., Kilbey B.
      Curr. Genet. 12:591-597(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.

    Entry informationi

    Entry nameiPUR2_SCHPO
    AccessioniPrimary (citable) accession number: P20772
    Secondary accession number(s): Q9UUM5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3