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P20772

- PUR2_SCHPO

UniProt

P20772 - PUR2_SCHPO

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Protein

Bifunctional purine biosynthetic protein ADE1

Gene

ade1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi291 – 2911ManganeseBy similarity
Metal bindingi293 – 2931ManganeseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi141 – 20262ATPBy similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoribosylamine-glycine ligase activity Source: PomBase
  4. phosphoribosylformylglycinamidine cyclo-ligase activity Source: PomBase

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: PomBase
  2. purine nucleobase biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_207552. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00125.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthetic protein ADE1
Including the following 2 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:ade1
ORF Names:SPBC405.01, SPBC4C3.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC405.01.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 788788Bifunctional purine biosynthetic protein ADE1PRO_0000074939Add
BLAST

Proteomic databases

MaxQBiP20772.
PaxDbiP20772.

Interactioni

Protein-protein interaction databases

BioGridi277549. 2 interactions.
MINTiMINT-4687567.
STRINGi4896.SPBC405.01-1.

Structurei

3D structure databases

ProteinModelPortaliP20772.
SMRiP20772. Positions 477-772.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini115 – 321207ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 430430GARSAdd
BLAST
Regioni440 – 750311AIRSAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the C-terminal section; belongs to the AIR synthase family.Curated
Contains 1 ATP-grasp domain.Curated

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000030315.
InParanoidiP20772.
KOiK11788.
OMAiGMLFTGF.
OrthoDBiEOG7Q8CXX.
PhylomeDBiP20772.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00138. GARS.
MF_00741. AIRS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20772-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEPIIALLIG NGGREHTIAW KLCESPLISK VYVAPGNGGT ASNGAESKME
60 70 80 90 100
NVNIGVCDFE QLVKFALDKD VNLVIPGPEL PLVEGIEGHF RRVGIPCFGP
110 120 130 140 150
SALAARMEGS KVFSKDFMHR NNIPTAVYKS FSNYDHAKSF LDTCTFDVVI
160 170 180 190 200
KADGLAAGKG VIIPKTKKEA FEALESIMLN EEFGSAGKNV VIEELLEGEE
210 220 230 240 250
LSILTFSDGY TCRSLPPAQD HKRAFDGDKG PNTGGMGCYA PTPVASPKLL
260 270 280 290 300
ETVQSTIIQP TIDGMRHEGY PLVGILFTGL MLTPSGPRVL EYNVRFGDPE
310 320 330 340 350
TQAVLPLLES DLAEIILACV NHRLDAIDIV ISRKFSCAVV CVAGGYPGPY
360 370 380 390 400
NKGDIITFDA LKDKNTRIFH AGTSIRDGNV VTNGGRVLAV EATGDSVEAA
410 420 430 440 450
VRLAYEGVKT VHFDKMFYRK DIAHHALNPK RKTREILTYE NSGVSVDNGN
460 470 480 490 500
EFVQRIKDLV KSTRRPGADA DIGGFGGIFD LKQAGWNDPL LVSATDGVGS
510 520 530 540 550
KLLIALSLNK HDTVGIDLVA MNVNDLVVQG AEPLIFLDYF ATGSLDLKVS
560 570 580 590 600
TSFVEGVVKG CKQAGCALVG GETSEMPGLY HDGHYDANGT SVGAVSRDDI
610 620 630 640 650
LPKPESFSKG DILLGLASDG VHSNGYSLVR KIVEYSDLEY TSVCPWDKNV
660 670 680 690 700
RLGDSLLIPT RIYVKPLLHV IRKNIVKGMA HITGGGLVEN VPRMLPSHLN
710 720 730 740 750
AIIDVDTWEV PEVFKWLKDA GNVPISDMAR TFNMGIGMVV AVASEDAEET
760 770 780
MKELTSVGET VYRIGQLVDK ESSSERCHLV NLNKWETF
Length:788
Mass (Da):85,231
Last modified:February 1, 1991 - v1
Checksum:i0FDE64EEA5F9095D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06601 Genomic DNA. Translation: CAA29820.1.
CU329671 Genomic DNA. Translation: CAA16823.1.
PIRiS00652.
RefSeqiNP_596304.1. NM_001022225.2.

Genome annotation databases

EnsemblFungiiSPBC405.01.1; SPBC405.01.1:pep; SPBC405.01.
GeneIDi2541034.
KEGGispo:SPBC405.01.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06601 Genomic DNA. Translation: CAA29820.1 .
CU329671 Genomic DNA. Translation: CAA16823.1 .
PIRi S00652.
RefSeqi NP_596304.1. NM_001022225.2.

3D structure databases

ProteinModelPortali P20772.
SMRi P20772. Positions 477-772.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277549. 2 interactions.
MINTi MINT-4687567.
STRINGi 4896.SPBC405.01-1.

Proteomic databases

MaxQBi P20772.
PaxDbi P20772.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC405.01.1 ; SPBC405.01.1:pep ; SPBC405.01 .
GeneIDi 2541034.
KEGGi spo:SPBC405.01.

Organism-specific databases

PomBasei SPBC405.01.

Phylogenomic databases

eggNOGi COG0151.
HOGENOMi HOG000030315.
InParanoidi P20772.
KOi K11788.
OMAi GMLFTGF.
OrthoDBi EOG7Q8CXX.
PhylomeDBi P20772.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
UPA00074 ; UER00129 .
Reactomei REACT_207552. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

NextBioi 20802148.
PROi P20772.

Family and domain databases

Gene3Di 3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPi MF_00138. GARS.
MF_00741. AIRS.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the bifunctional ade1 gene in the purine biosynthetic pathway of the fission yeast Schizosaccharomyces pombe."
    McKenzie R., Schuchert P., Kilbey B.
    Curr. Genet. 12:591-597(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiPUR2_SCHPO
AccessioniPrimary (citable) accession number: P20772
Secondary accession number(s): Q9UUM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 26, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3