Reviewed,
UniProtKB/Swiss-Prot P20772 (PUR2_SCHPO)
Last modified
February 9, 2010.
Version 81.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Bifunctional purine biosynthetic protein ADE1 Including the following 2 domains: 1- Recommended name: Phosphoribosylamine--glycine ligase EC=6.3.4.13 Alternative name(s): Glycinamide ribonucleotide synthetase Short name=GARS Phosphoribosylglycinamide synthetase 2- Recommended name: Phosphoribosylformylglycinamidine cyclo-ligase EC=6.3.3.1 Alternative name(s): Phosphoribosyl-aminoimidazole synthetase AIR synthase Short name=AIRS | ||||
| Gene names |
| ||||
| Organism | Schizosaccharomyces pombe (Fission yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4896 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces |
Protein attributes
| Sequence length | 788 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. |
| Pathway | |
| Sequence similarities | In the N-terminal section; belongs to the GARS family. In the C-terminal section; belongs to the AIR synthase family. Contains 1 ATP-grasp domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Purine biosynthesis |
| Ligand | ATP-binding Manganese Metal-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | 'de novo' IMP biosynthetic process Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe purine base biosynthetic processInferred from electronic annotation. Source: InterPro |
| Cellular component | cytosol Inferred from direct assay. Source: GeneDB_SPombe |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Ref.1Inferred from mutant phenotype. Source: GeneDB_SPombe phosphoribosylformylglycinamidine cyclo-ligase activity Ref.1Inferred from mutant phenotype. Source: GeneDB_SPombe |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 788 | 788 | Bifunctional purine biosynthetic protein ADE1 | PRO_0000074939 | |||||
Regions | |||||||||
| Domain | 115 – 321 | 207 | ATP-grasp | ||||||
| Nucleotide binding | 141 – 202 | 62 | ATP By similarity | ||||||
| Region | 1 – 430 | 430 | GARS | ||||||
| Region | 440 – 750 | 311 | AIRS | ||||||
Sites | |||||||||
| Metal binding | 291 | 1 | Manganese By similarity | ||||||
| Metal binding | 293 | 1 | Manganese By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence of the bifunctional ade1 gene in the purine biosynthetic pathway of the fission yeast Schizosaccharomyces pombe." McKenzie R., Schuchert P., Kilbey B. Curr. Genet. 12:591-597(1987) [PubMed: 3502942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38366 / 972. |
| [2] | "The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P.Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X06601 Genomic DNA. Translation: CAA29820.1. CU329671 Genomic DNA. Translation: CAA16823.1. |
| PIR | S00652. |
| RefSeq | NP_596304.1. |
3D structure databases | |
| SMR | P20772. Positions 6-427, 436-772. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P20772. |
Genome annotation databases | |
| GeneID | 2541034. |
| GenomeReviews | Gene locus ade1 in contig CU329671_GR. |
| KEGG | spo:SPBC405.01. |
| NMPDR | fig|4896.1.peg.2170. |
Organism-specific databases | |
| GeneDB_Spombe | SPBC405.01. |
Phylogenomic databases | |
| eggNOG | fuNOG04381. |
| HOGENOM | HBG328156. |
| OMA | GETSEMP. |
| OrthoDB | EOG9869VQ. |
| PhylomeDB | P20772. |
Enzyme and pathway databases | |
| BRENDA | 6.3.3.1. 653. 6.3.4.13. 653. |
Gene expression databases | |
| ArrayExpress | P20772. |
Family and domain databases | |
| InterPro | IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. IPR011054. Rudment_hybrid_motif. [Graphical view] |
| Gene3D | G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit. |
| Pfam | PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00877. purD. 1 hit. TIGR00878. purM. 1 hit. |
| PROSITE | PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PUR2_SCHPO | ||||||||
| Accession | Primary (citable) accession number: P20772 Secondary accession number(s): Q9UUM5 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| Schizosaccharomyces pombe Schizosaccharomyces pombe: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

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