P20772 (PUR2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional purine biosynthetic protein ADE1

Including the following 2 domains:

1. Phosphoribosylamine--glycine ligase
   EC=6.3.4.13
   Alternative name(s):
   Glycinamide ribonucleotide synthetase
   Short name=GARS
   Phosphoribosylglycinamide synthetase
2. Phosphoribosylformylglycinamidine cyclo-ligase
   EC=6.3.3.1
   Alternative name(s):
   AIR synthase
   Short name=AIRS
   Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	ade1
ORF Names:	SPBC405.01, SPBC4C3.02c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	788 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.
Sequence similarities	In the N-terminal section; belongs to the GARS family. In the C-terminal section; belongs to the AIR synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from mutant phenotype Ref.1. Source: PomBase purine nucleobase biosynthetic process Inferred from direct assay PubMed 967158. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from direct assay PubMed 967158. Source: PomBase phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from direct assay PubMed 967158. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 788	788	Bifunctional purine biosynthetic protein ADE1		PRO_0000074939
Regions
	Domain	115 – 321	207	ATP-grasp
	Nucleotide binding	141 – 202	62	ATP By similarity
	Region	1 – 430	430	GARS
	Region	440 – 750	311	AIRS
Sites
	Metal binding	291	1	Manganese By similarity
	Metal binding	293	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20772 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 0FDE64EEA5F9095D	FASTA	788	85,231
	10 20 30 40 50 60 MEPIIALLIG NGGREHTIAW KLCESPLISK VYVAPGNGGT ASNGAESKME NVNIGVCDFE 70 80 90 100 110 120 QLVKFALDKD VNLVIPGPEL PLVEGIEGHF RRVGIPCFGP SALAARMEGS KVFSKDFMHR 130 140 150 160 170 180 NNIPTAVYKS FSNYDHAKSF LDTCTFDVVI KADGLAAGKG VIIPKTKKEA FEALESIMLN 190 200 210 220 230 240 EEFGSAGKNV VIEELLEGEE LSILTFSDGY TCRSLPPAQD HKRAFDGDKG PNTGGMGCYA 250 260 270 280 290 300 PTPVASPKLL ETVQSTIIQP TIDGMRHEGY PLVGILFTGL MLTPSGPRVL EYNVRFGDPE 310 320 330 340 350 360 TQAVLPLLES DLAEIILACV NHRLDAIDIV ISRKFSCAVV CVAGGYPGPY NKGDIITFDA 370 380 390 400 410 420 LKDKNTRIFH AGTSIRDGNV VTNGGRVLAV EATGDSVEAA VRLAYEGVKT VHFDKMFYRK 430 440 450 460 470 480 DIAHHALNPK RKTREILTYE NSGVSVDNGN EFVQRIKDLV KSTRRPGADA DIGGFGGIFD 490 500 510 520 530 540 LKQAGWNDPL LVSATDGVGS KLLIALSLNK HDTVGIDLVA MNVNDLVVQG AEPLIFLDYF 550 560 570 580 590 600 ATGSLDLKVS TSFVEGVVKG CKQAGCALVG GETSEMPGLY HDGHYDANGT SVGAVSRDDI 610 620 630 640 650 660 LPKPESFSKG DILLGLASDG VHSNGYSLVR KIVEYSDLEY TSVCPWDKNV RLGDSLLIPT 670 680 690 700 710 720 RIYVKPLLHV IRKNIVKGMA HITGGGLVEN VPRMLPSHLN AIIDVDTWEV PEVFKWLKDA 730 740 750 760 770 780 GNVPISDMAR TFNMGIGMVV AVASEDAEET MKELTSVGET VYRIGQLVDK ESSSERCHLV NLNKWETF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the bifunctional ade1 gene in the purine biosynthetic pathway of the fission yeast Schizosaccharomyces pombe." McKenzie R., Schuchert P., Kilbey B. Curr. Genet. 12:591-597(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06601 Genomic DNA. Translation: CAA29820.1. CU329671 Genomic DNA. Translation: CAA16823.1.
PIR	S00652.
RefSeq	NP_596304.1. NM_001022225.2.
3D structure databases
ProteinModelPortal	P20772.
SMR	P20772. Positions 477-772.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC405.01-1.
Proteomic databases
PaxDb	P20772.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC405.01.1; SPBC405.01.1:pep; SPBC405.01.
GeneID	2541034.
KEGG	spo:SPBC405.01.
Organism-specific databases
PomBase	SPBC405.01.
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HOG000030315.
KO	K11788.
OMA	GETSEMP.
OrthoDB	EOG48PQTB.
Enzyme and pathway databases
UniPathway	UPA00074; UER00125. UPA00074; UER00129.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR004733. PurM_cligase. IPR016188. PurM_N-like. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF52440. PreATP-grasp-like. 1 hit. SSF55326. PurM_N-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit. TIGR00878. purM. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20802148.

Entry information

Entry name

PUR2_SCHPO

Accession

Primary (citable) accession number: P20772
Secondary accession number(s): Q9UUM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program