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Protein

B-cell lymphoma 3 protein

Gene

BCL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity).By similarity

GO - Molecular functioni

  1. DNA binding Source: Ensembl
  2. protein binding, bridging Source: UniProtKB
  3. sequence-specific DNA binding transcription factor activity Source: Ensembl
  4. transcription factor binding Source: UniProtKB

GO - Biological processi

  1. antimicrobial humoral response Source: Ensembl
  2. cellular response to DNA damage stimulus Source: UniProtKB
  3. defense response to bacterium Source: Ensembl
  4. defense response to protozoan Source: Ensembl
  5. DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
  6. extracellular matrix organization Source: Ensembl
  7. follicular dendritic cell differentiation Source: Ensembl
  8. germinal center formation Source: Ensembl
  9. humoral immune response mediated by circulating immunoglobulin Source: Ensembl
  10. I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  11. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: MGI
  12. maintenance of protein location in nucleus Source: UniProtKB
  13. marginal zone B cell differentiation Source: Ensembl
  14. negative regulation of apoptotic process Source: UniProtKB
  15. negative regulation of interleukin-8 biosynthetic process Source: UniProtKB
  16. negative regulation of transcription, DNA-templated Source: UniProtKB
  17. negative regulation of tumor necrosis factor biosynthetic process Source: Ensembl
  18. positive regulation of interferon-gamma production Source: Ensembl
  19. positive regulation of interleukin-10 biosynthetic process Source: Ensembl
  20. positive regulation of transcription, DNA-templated Source: UniProtKB
  21. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  22. positive regulation of translation Source: UniProtKB
  23. protein import into nucleus, translocation Source: UniProtKB
  24. regulation of apoptotic process Source: UniProtKB
  25. regulation of DNA binding Source: UniProtKB
  26. regulation of NF-kappaB import into nucleus Source: UniProtKB
  27. response to UV-C Source: UniProtKB
  28. response to virus Source: UniProtKB
  29. spleen development Source: Ensembl
  30. T-helper 1 type immune response Source: Ensembl
  31. T-helper 2 cell differentiation Source: Ensembl
  32. transcription, DNA-templated Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
B-cell lymphoma 3 protein
Short name:
BCL-3
Alternative name(s):
Proto-oncogene BCL3
Gene namesi
Name:BCL3
Synonyms:BCL4, D19S37
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:998. BCL3.

Subcellular locationi

Nucleus. Cytoplasm By similarity. Cytoplasmperinuclear region By similarity
Note: Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.By similarity

GO - Cellular componenti

  1. Bcl3/NF-kappaB2 complex Source: UniProtKB
  2. Bcl3-Bcl10 complex Source: UniProtKB
  3. cytoplasm Source: UniProtKB
  4. intercellular bridge Source: HPA
  5. nucleoplasm Source: HPA
  6. nucleus Source: UniProtKB
  7. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  8. protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

PharmGKBiPA25310.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454B-cell lymphoma 3 proteinPRO_0000066976Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei402 – 4021Phosphoserine; by GSK31 Publication
Modified residuei406 – 4061Phosphoserine; by GSK31 Publication

Post-translational modificationi

Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation (By similarity).By similarity
Activated by phosphorylation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP20749.
PaxDbiP20749.
PRIDEiP20749.

PTM databases

PhosphoSiteiP20749.

Expressioni

Gene expression databases

BgeeiP20749.
CleanExiHS_BCL3.
GenevestigatoriP20749.

Organism-specific databases

HPAiCAB002051.
HPA047514.

Interactioni

Subunit structurei

Component of a complex consisting of the NF-kappa-B p52-p52 homodimer and BCL3. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Interacts with N4BP2, COPS5 and PIR. Interacts with CYLD (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BCL10O959993EBI-958997,EBI-958922
CTBP2P565452EBI-958997,EBI-741533
LCKP062393EBI-958997,EBI-1348

Protein-protein interaction databases

BioGridi107074. 43 interactions.
IntActiP20749. 10 interactions.
MINTiMINT-105740.
STRINGi9606.ENSP00000164227.

Structurei

Secondary structure

1
454
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi138 – 1447Combined sources
Helixi148 – 16013Combined sources
Helixi175 – 1817Combined sources
Helixi185 – 1939Combined sources
Helixi208 – 2147Combined sources
Helixi218 – 22710Combined sources
Helixi245 – 2528Combined sources
Helixi255 – 2639Combined sources
Turni273 – 2753Combined sources
Helixi279 – 2857Combined sources
Helixi289 – 2979Combined sources
Helixi312 – 3198Combined sources
Helixi322 – 3309Combined sources
Turni345 – 3484Combined sources
Helixi352 – 3587Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K1AX-ray1.86A127-367[»]
1K1BX-ray1.90A127-367[»]
ProteinModelPortaliP20749.
SMRiP20749. Positions 133-432.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20749.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati134 – 16330ANK 1Add
BLAST
Repeati171 – 20030ANK 2Add
BLAST
Repeati204 – 23532ANK 3Add
BLAST
Repeati241 – 27030ANK 4Add
BLAST
Repeati275 – 30430ANK 5Add
BLAST
Repeati308 – 33730ANK 6Add
BLAST
Repeati338 – 36730ANK 7Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi9 – 120112Pro-richAdd
BLAST
Compositional biasi365 – 45490Pro/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 7 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00550000074527.
HOGENOMiHOG000095189.
HOVERGENiHBG108320.
InParanoidiP20749.
KOiK09258.
OMAiPLYPMMC.
OrthoDBiEOG7W154S.
PhylomeDBiP20749.
TreeFamiTF320166.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 4 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20749-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRCPAGAMD EGPVDLRTRP KAAGLPGAAL PLRKRPLRAP SPEPAAPRGA
60 70 80 90 100
AGLVVPLDPL RGGCDLPAVP GPPHGLARPE ALYYPGALLP LYPTRAMGSP
110 120 130 140 150
FPLVNLPTPL YPMMCPMEHP LSADIAMATR ADEDGDTPLH IAVVQGNLPA
160 170 180 190 200
VHRLVNLFQQ GGRELDIYNN LRQTPLHLAV ITTLPSVVRL LVTAGASPMA
210 220 230 240 250
LDRHGQTAAH LACEHRSPTC LRALLDSAAP GTLDLEARNY DGLTALHVAV
260 270 280 290 300
NTECQETVQL LLERGADIDA VDIKSGRSPL IHAVENNSLS MVQLLLQHGA
310 320 330 340 350
NVNAQMYSGS SALHSASGRG LLPLVRTLVR SGADSSLKNC HNDTPLMVAR
360 370 380 390 400
SRRVIDILRG KATRPASTSQ PDPSPDRSAN TSPESSSRLS SNGLLSASPS
410 420 430 440 450
SSPSQSPPRD PPGFPMAPPN FFLPSPSPPA FLPFAGVLRG PGRPVPPSPA

PGGS
Length:454
Mass (Da):47,584
Last modified:April 19, 2010 - v2
Checksum:i385F5320DB72C0E0
GO

Sequence cautioni

The sequence AAA51815.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAA51816.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH64993.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31731 Genomic DNA. Translation: AAA51816.1. Different initiation.
M31732 mRNA. Translation: AAA51815.1. Different initiation.
AC092066 Genomic DNA. No translation available.
BC064993 mRNA. Translation: AAH64993.1. Different initiation.
AH006679 Genomic DNA. Translation: AAC51348.1.
CCDSiCCDS12642.2.
PIRiA34794.
RefSeqiNP_005169.2. NM_005178.4.
UniGeneiHs.31210.

Genome annotation databases

EnsembliENST00000164227; ENSP00000164227; ENSG00000069399.
GeneIDi602.
KEGGihsa:602.
UCSCiuc010xxe.2. human.

Polymorphism databases

DMDMi294862410.

Keywords - Coding sequence diversityi

Chromosomal rearrangement

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31731 Genomic DNA. Translation: AAA51816.1. Different initiation.
M31732 mRNA. Translation: AAA51815.1. Different initiation.
AC092066 Genomic DNA. No translation available.
BC064993 mRNA. Translation: AAH64993.1. Different initiation.
AH006679 Genomic DNA. Translation: AAC51348.1.
CCDSiCCDS12642.2.
PIRiA34794.
RefSeqiNP_005169.2. NM_005178.4.
UniGeneiHs.31210.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K1AX-ray1.86A127-367[»]
1K1BX-ray1.90A127-367[»]
ProteinModelPortaliP20749.
SMRiP20749. Positions 133-432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107074. 43 interactions.
IntActiP20749. 10 interactions.
MINTiMINT-105740.
STRINGi9606.ENSP00000164227.

PTM databases

PhosphoSiteiP20749.

Polymorphism databases

DMDMi294862410.

Proteomic databases

MaxQBiP20749.
PaxDbiP20749.
PRIDEiP20749.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000164227; ENSP00000164227; ENSG00000069399.
GeneIDi602.
KEGGihsa:602.
UCSCiuc010xxe.2. human.

Organism-specific databases

CTDi602.
GeneCardsiGC19P045250.
HGNCiHGNC:998. BCL3.
HPAiCAB002051.
HPA047514.
MIMi109560. gene.
neXtProtiNX_P20749.
PharmGKBiPA25310.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00550000074527.
HOGENOMiHOG000095189.
HOVERGENiHBG108320.
InParanoidiP20749.
KOiK09258.
OMAiPLYPMMC.
OrthoDBiEOG7W154S.
PhylomeDBiP20749.
TreeFamiTF320166.

Miscellaneous databases

EvolutionaryTraceiP20749.
GeneWikiiBCL3.
GenomeRNAii602.
NextBioi2447.
PROiP20749.
SOURCEiSearch...

Gene expression databases

BgeeiP20749.
CleanExiHS_BCL3.
GenevestigatoriP20749.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 4 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The candidate proto-oncogene bcl-3 is related to genes implicated in cell lineage determination and cell cycle control."
    Ohno H., Takimoto G., McKeithan T.W.
    Cell 60:991-997(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  3. "Genomic structure of the candidate proto-oncogene BCL3."
    McKeithan T.W., Ohno H., Dickstein J., Hume E.
    Genomics 24:120-126(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 87-454.
    Tissue: Leukemia.
  4. "The oncoprotein Bcl-3 directly transactivates through kappa B motifs via association with DNA-binding p50B homodimers."
    Bours V., Franzoso G., Azarenko V., Park S., Kanno T., Brown K., Siebenlist U.
    Cell 72:729-739(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH NFKB2/P52.
  5. "NF-kappaB p105 is a target of IkappaB kinases and controls signal induction of Bcl-3-p50 complexes."
    Heissmeyer V., Krappmann D., Wulczyn F.G., Scheidereit C.
    EMBO J. 18:4766-4778(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH NFKB1/P50.
  6. "The Bcl-3 oncoprotein acts as a bridging factor between NF-kappaB/Rel and nuclear co-regulators."
    Dechend R., Hirano F., Lehmann K., Heissmeyer V., Ansieau S., Wulczyn F.G., Scheidereit C., Leutz A.
    Oncogene 18:3316-3323(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH COPS5 AND PIR.
  7. "Identification and characterization of BCL-3-binding protein: implications for transcription and DNA repair or recombination."
    Watanabe N., Wachi S., Fujita T.
    J. Biol. Chem. 278:26102-26110(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH N4BP2.
  8. "Crystal structure of the ankyrin repeat domain of Bcl-3: a unique member of the IkappaB protein family."
    Michel F., Soler-Lopez M., Petosa C., Cramer P., Siebenlist U., Muller C.W.
    EMBO J. 20:6180-6190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) OF 127-367.
  9. Cited for: PHOSPHORYLATION AT SER-402 AND SER-406.

Entry informationi

Entry nameiBCL3_HUMAN
AccessioniPrimary (citable) accession number: P20749
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1991
Last sequence update: April 19, 2010
Last modified: March 3, 2015
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.