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P20742 (PZP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pregnancy zone protein
Alternative name(s):
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6
Gene names
Name:PZP
Synonyms:CPAMD6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1482 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Subunit structure

Homotetramer, which consists of two pairs of disulfide-linked chains.

Subcellular location

Secreted.

Tissue specificity

Plasma. Prominent constituent of late-pregnancy sera.

Sequence similarities

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P20742-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P20742-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-131: Missing.
     132-142: TDKPMYKPGQT → MSESYRRTTFP
     830-912: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.4
Chain26 – 14821457Pregnancy zone protein
PRO_0000000063

Regions

Region685 – 73551Bait region

Amino acid modifications

Glycosylation241N-linked (GlcNAc...) Potential
Glycosylation541N-linked (GlcNAc...) Potential
Glycosylation691N-linked (GlcNAc...) Potential
Glycosylation2461N-linked (GlcNAc...) Potential
Glycosylation3921N-linked (GlcNAc...) Potential
Glycosylation4061N-linked (GlcNAc...) Ref.10
Glycosylation7531N-linked (GlcNAc...) Potential
Glycosylation8751N-linked (GlcNAc...) Potential
Glycosylation9321N-linked (GlcNAc...) Ref.10
Glycosylation9971N-linked (GlcNAc...) Ref.10 Ref.11
Glycosylation14301N-linked (GlcNAc...) Ref.10
Cross-link978 ↔ 981Isoglutamyl cysteine thioester (Cys-Gln)

Natural variations

Alternative sequence1 – 131131Missing in isoform 2.
VSP_030862
Alternative sequence132 – 14211TDKPMYKPGQT → MSESYRRTTFP in isoform 2.
VSP_030863
Alternative sequence830 – 91283Missing in isoform 2.
VSP_030864
Natural variant3791L → V.
Corresponds to variant rs12230214 [ dbSNP | Ensembl ].
VAR_034429
Natural variant6911V → M. Ref.5
Corresponds to variant rs3213832 [ dbSNP | Ensembl ].
VAR_021845
Natural variant8131V → A.
Corresponds to variant rs2277413 [ dbSNP | Ensembl ].
VAR_020005
Natural variant8571N → S. Ref.1
Corresponds to variant rs3213831 [ dbSNP | Ensembl ].
VAR_060733
Natural variant10031T → M.
Corresponds to variant rs57006764 [ dbSNP | Ensembl ].
VAR_060982
Natural variant11281R → H in a colorectal cancer sample; somatic mutation. Ref.13
VAR_036235
Natural variant12051T → P. Ref.3
Corresponds to variant rs2377741 [ dbSNP | Ensembl ].
VAR_060734
Natural variant14431I → N.
Corresponds to variant rs10842971 [ dbSNP | Ensembl ].
VAR_024358

Experimental info

Sequence conflict7531N → Q AA sequence Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 15, 2009. Version 4.
Checksum: FA89A47458C4A18B

FASTA1,482163,863
        10         20         30         40         50         60 
MRKDRLLHLC LVLLLILLSA SDSNSTEPQY MVLVPSLLHT EAPKKGCVLL SHLNETVTVS 

        70         80         90        100        110        120 
ASLESGRENR SLFTDLVAEK DLFHCVSFTL PRISASSEVA FLSIQIKGPT QDFRKRNTVL 

       130        140        150        160        170        180 
VLNTQSLVFV QTDKPMYKPG QTVRFRVVSV DENFRPRNEL IPLIYLENPR RNRIAQWQSL 

       190        200        210        220        230        240 
KLEAGINQLS FPLSSEPIQG SYRVVVQTES GGRIQHPFTV EEFVLPKFEV KVQVPKIISI 

       250        260        270        280        290        300 
MDEKVNITVC GEYTYGKPVP GLATVSLCRK LSRVLNCDKQ EVCEEFSQQL NSNGCITQQV 

       310        320        330        340        350        360 
HTKMLQITNT GFEMKLRVEA RIREEGTDLE VTANRISEIT NIVSKLKFVK VDSHFRQGIP 

       370        380        390        400        410        420 
FFAQVLLVDG KGVPIPNKLF FISVNDANYY SNATTNEQGL AQFSINTTSI SVNKLFVRVF 

       430        440        450        460        470        480 
TVHPNLCFHY SWVAEDHQGA QHTANRVFSL SGSYIHLEPV AGTLPCGHTE TITAHYTLNR 

       490        500        510        520        530        540 
QAMGELSELS FHYLIMAKGV IVRSGTHTLP VESGDMKGSF ALSFPVESDV APIARMFIFA 

       550        560        570        580        590        600 
ILPDGEVVGD SEKFEIENCL ANKVDLSFSP AQSPPASHAH LQVAAAPQSL CALRAVDQSV 

       610        620        630        640        650        660 
LLMKPEAELS VSSVYNLLTV KDLTNFPDNV DQQEEEQGHC PRPFFIHNGA IYVPLSSNEA 

       670        680        690        700        710        720 
DIYSFLKGMG LKVFTNSKIR KPKSCSVIPS VSAGAVGQGY YGAGLGVVER PYVPQLGTYN 

       730        740        750        760        770        780 
VIPLNNEQSS GPVPETVRSY FPETWIWELV AVNSSGVAEV GVTVPDTITE WKAGAFCLSE 

       790        800        810        820        830        840 
DAGLGISSTA SLRAFQPFFV ELTMPYSVIR GEVFTLKATV LNYLPKCIRV SVQLKASPAF 

       850        860        870        880        890        900 
LASQNTKGEE SYCICGNERQ TLSWTVTPKT LGNVNFSVSA EAMQSLELCG NEVVEVPEIK 

       910        920        930        940        950        960 
RKDTVIKTLL VEAEGIEQEK TFSSMTCASG ANVSEQLSLK LPSNVVKESA RASFSVLGDI 

       970        980        990       1000       1010       1020 
LGSAMQNIQN LLQMPYGCGE QNMVLFAPNI YVLNYLNETQ QLTQEIKAKA VGYLITGYQR 

      1030       1040       1050       1060       1070       1080 
QLNYKHQDGS YSTFGERYGR NQGNTWLTAF VLKTFAQARS YIFIDEAHIT QSLTWLSQMQ 

      1090       1100       1110       1120       1130       1140 
KDNGCFRSSG SLLNNAIKGG VEDEATLSAY VTIALLEIPL PVTNPIVRNA LFCLESAWNV 

      1150       1160       1170       1180       1190       1200 
AKEGTHGSHV YTKALLAYAF SLLGKQNQNR EILNSLDKEA VKEDNLVHWE RPQRPKAPVG 

      1210       1220       1230       1240       1250       1260 
HLYQTQAPSA EVEMTSYVLL AYLTAQPAPT SGDLTSATNI VKWIMKQQNA QGGFSSTQDT 

      1270       1280       1290       1300       1310       1320 
VVALHALSRY GAATFTRTEK TAQVTVQDSQ TFSTNFQVDN NNLLLLQQIS LPELPGEYVI 

      1330       1340       1350       1360       1370       1380 
TVTGERCVYL QTSMKYNILP EKEDSPFALK VQTVPQTCDG HKAHTSFQIS LTISYTGNRP 

      1390       1400       1410       1420       1430       1440 
ASNMVIVDVK MVSGFIPLKP TVKMLERSSS VSRTEVSNNH VLIYVEQVTN QTLSFSFMVL 

      1450       1460       1470       1480 
QDIPVGDLKP AIVKVYDYYE TDESVVAEYI APCSTDTEHG NV 

« Hide

Isoform 2 [UniParc].

Checksum: 8D3255FD5698667E
Show »

FASTA1,268140,368

References

« Hide 'large scale' references
[1]"Primary structure of pregnancy zone protein. Molecular cloning of a full-length PZP cDNA clone by the polymerase chain reaction."
Devriendt K., van den Berghe H., Cassiman J.-J., Marynen P.
Biochim. Biophys. Acta 1088:95-103(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT SER-857.
[2]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-1205.
[4]"Alpha-macroglobulin domain structure studied by specific limited proteolysis."
Thomsen N.K., Sottrup-Jensen L.
Arch. Biochem. Biophys. 300:327-334(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-37; 222-230; 720-733; 896-902; 1003-1008; 1056-1061; 1184-1193; 1197-1224; 1277-1286 AND 1336-1344 (ISOFORM 1).
[5]"A genetic polymorphism in a functional domain of human pregnancy zone protein: the bait region. Genomic structure of the bait domains of human pregnancy zone protein and alpha 2 macroglobulin."
Marynen P., Devriendt K., van den Berghe H., Cassiman J.-J.
FEBS Lett. 262:349-352(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 668-753, VARIANT MET-691.
Tissue: Placenta.
[6]"The alpha-macroglobulin bait region. Sequence diversity and localization of cleavage sites for proteinases in five mammalian alpha-macroglobulins."
Sottrup-Jensen L., Sand O., Kristensen L., Fey G.H.
J. Biol. Chem. 264:15781-15789(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 670-759.
[7]"Characterization of human pregnancy zone protein. Comparison with human alpha 2-macroglobulin."
Sand O., Folkersen J., Westergaard J.G., Sottrup-Jensen L.
J. Biol. Chem. 260:15723-15735(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 974-983.
[8]"A cluster of alpha 2-macroglobulin-related genes (alpha 2 M) on human chromosome 12p: cloning of the pregnancy-zone protein gene and an alpha 2M pseudogene."
Devriendt K., Zhang J., van Leuven F., van den Berghe H., Cassiman J.-J., Marynen P.
Gene 81:325-334(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1259-1461.
[9]"Pregnancy zone protein, a proteinase-binding macroglobulin. Interactions with proteinases and methylamine."
Christensen U., Simonsen M., Harrit N., Sottrup-Jensen L.
Biochemistry 28:9324-9331(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PROTEINASES AND METHYLAMINE.
[10]"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-406; ASN-932; ASN-997 AND ASN-1430.
Tissue: Plasma.
[11]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-997.
Tissue: Liver.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-1128.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54380 mRNA. Translation: CAA38255.1.
AC010175 Genomic DNA. No translation available.
BC111756 mRNA. Translation: AAI11757.1.
X51541 Genomic DNA. Translation: CAA35919.1.
M24416 Genomic DNA. Translation: AAA60234.1.
PIRS13495.
S29738.
UniGeneHs.707491.

3D structure databases

ProteinModelPortalP20742.
SMRP20742. Positions 126-226, 1346-1474.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000261336.

Protein family/group databases

MEROPSI39.003.

PTM databases

PhosphoSiteP20742.

Polymorphism databases

DMDM281185515.

Proteomic databases

PaxDbP20742.
PRIDEP20742.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000261336; ENSP00000261336; ENSG00000126838. [P20742-1]
ENST00000381997; ENSP00000371427; ENSG00000126838. [P20742-2]
UCSCuc001qvl.3. human. [P20742-1]
uc009zgl.3. human. [P20742-2]

Organism-specific databases

GeneCardsGC12M009301.
H-InvDBHIX0036869.
HGNCHGNC:9750. PZP.
HPAHPA041403.
HPA041471.
MIM176420. gene.
neXtProtNX_P20742.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2373.
HOGENOMHOG000220939.
HOVERGENHBG000039.
InParanoidP20742.
OMAWIWELVA.
OrthoDBEOG7DJSKB.
PhylomeDBP20742.
TreeFamTF313285.

Gene expression databases

ArrayExpressP20742.
BgeeP20742.
GenevestigatorP20742.

Family and domain databases

Gene3D1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR010916. TonB_box_CS.
[Graphical view]
PfamPF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP20742.
SOURCESearch...

Entry information

Entry namePZP_HUMAN
AccessionPrimary (citable) accession number: P20742
Secondary accession number(s): A6ND27 expand/collapse secondary AC list , Q15273, Q2NKL2, Q7M4N7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 15, 2009
Last modified: April 16, 2014
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM