Reviewed, UniProtKB/Swiss-Prot P20720 (UD12_RAT)

Names and origin

Protein names

Recommended name:
    UDP-glucuronosyltransferase 1-2
      Short name=UDPGT
    EC=2.4.1.17
Alternative name(s):
    UGT1*0
    UGT1-02
      Short name=UGT1.2
    UGT1A2
    Bilirubin specific
    B2

Gene names

Name:

Ugt1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds.
Catalytic activity	UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.
Subcellular location	Microsome. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucuronosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. The different isozymes have a different N-terminal domain and a common C-terminal domain of 245 residues.

Isoform 1 (identifier: P20720-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 533	506	UDP-glucuronosyltransferase 1-2		PRO_0000036013
Regions
	Transmembrane	491 – 507	17	Potential
Amino acid modifications
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	141	1	N-linked (GlcNAc...) Potential
	Glycosylation	295	1	N-linked (GlcNAc...) Potential
	Glycosylation	433	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	2 – 3	2	Missing Ref.1
	Sequence conflict	177 – 178	2	CG → SP in BAA07261. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 5A9E974EF7EBAF5B	FASTA	533	60,045
	10 20 30 40 50 60 MDTGLCAPLR GLSGLLLLLC ALPWAEGGKV LVFPMEGSHW LSMRDVVREL HARGHQAVVL 70 80 90 100 110 120 APEVTVHMKG EDFFTLQTYA FPYTKEEYQR EILGNAKKGF EPQHFVKTFF ETMASIKKFF 130 140 150 160 170 180 DLYANSCAAL LHNKTLIQQL NSSSFDVVLT DPVFPCGALL AKYLQIPAVF FLRSVPCGID 190 200 210 220 230 240 YEATQCPKPS SYIPNLLTML SDHMTFLQRV KNMLYPLTLK YICHLSITPY ESLASELLQR 250 260 270 280 290 300 EMSLVEVLSH ASVWLFRGDF VFDYPRPIMP NMVFIGGINC VIKKPLSQEF EAYVNASGEH 310 320 330 340 350 360 GIVVFSLGSM VSEIPEKKAM EIAEALGRIP QTLLWRYTGT RPSNLAKNTI LVKWLPQNDL 370 380 390 400 410 420 LGHPKARAFI THSGSHGIYE GICNGVPMVM MPLFGDQMDN AKRMETRGAG VTLNVLEMTA 430 440 450 460 470 480 DDLENALKTV INNKSYKENI MRLSSLHKDR PIEPLDLAVF WVEYVMRHKG APHLRPAAHD 490 500 510 520 530 LTWYQYHSLD VIGFLLAIVL TVVFIVYKSC AYGCRKCFGG KGRVKKSHKS KTH

References

[1]	"Isolation and sequencing of rat liver bilirubin UDP-glucuronosyltransferase cDNA: possible alternate splicing of a common primary transcript." Sato H., Koiwai O., Tanabe K., Kashiwamata S. Biochem. Biophys. Res. Commun. 169:260-264(1990) [PubMed: 2112380] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Drug-responsive and tissue-specific alternative expression of multiple first exons in rat UDP-glucuronosyltransferase family 1 (UGT1) gene complex." Emi Y., Ikushiro S., Iyanagi T. J. Biochem. 117:392-399(1995) [PubMed: 7608130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-288. Strain: Wistar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M34007 mRNA. Translation: AAA42312.1. D38066 Genomic DNA. Translation: BAA07261.1.
IPI	IPI00202325.
PIR	A35343.
UniGene	Rn.26489
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Genome annotation databases
Ensembl	ENSRNOG00000018740. Rattus norvegicus. [Contig view]
Phylogenomic databases
HOVERGEN	P20720.
Enzyme and pathway databases
BRENDA	2.4.1.17. 248.
Gene expression databases
ArrayExpress	P20720.
GermOnline	ENSRNOG00000018740. Rattus norvegicus.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UD12_RAT

Accession

Primary (citable) accession number: P20720
Secondary accession number(s): Q64636

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)