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Reviewed, UniProtKB/Swiss-Prot P20718 (GRAH_HUMAN)

Last modified January 19, 2010. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Granzyme H
    EC=3.4.21.-
Alternative name(s):
    Cytotoxic T-lymphocyte proteinase
    Cathepsin G-like 2
      Short name=CTSGL2
    CCP-X
    Cytotoxic serine protease C
      Short name=CSP-C
Gene names
Name: GZMH
Synonyms: CGL2, CTSGL2
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

This enzyme is probably necessary for target cell lysis in cell-mediated immune responses.

Subcellular location

Cytoplasmic granule. Note: Cytoplasmic granules of cytolytic T-lymphocytes.

Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Contains 1 peptidase S1 domain.

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Ontologies

Keywords
   Biological processCytolysis
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processapoptosis Ref.1 Ref.3

Non-traceable author statement. Source: UniProtKB

cytolysis

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Non-traceable author statement. Source: UniProtKB

   Cellular componentcytoplasm

Non-traceable author statement. Source: UniProtKB

   Molecular functionserine-type endopeptidase activity Ref.1 Ref.3

Non-traceable author statement. Source: UniProtKB

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818
Propeptide19 – 202Activation peptide
PRO_0000027413
Chain21 – 246226Granzyme H
PRO_0000027414

Regions

Domain21 – 244224Peptidase S1

Sites

Active site641Charge relay system By similarity
Active site1081Charge relay system By similarity
Active site2021Charge relay system By similarity

Amino acid modifications

Glycosylation711N-linked (GlcNAc...) Potential
Glycosylation1041N-linked (GlcNAc...) Potential
Glycosylation1791N-linked (GlcNAc...) Potential
Disulfide bond49 ↔ 65 By similarity
Disulfide bond142 ↔ 208 By similarity
Disulfide bond172 ↔ 187 By similarity

Natural variations

Natural variant841R → Q: dbSNP rs20545.
VAR_014556

Secondary structure

1 ......................................... 246
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P20718-1 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: CA6A87F3DA5F1E71

FASTA24627,315
        10         20         30         40         50         60 
MQPFLLLLAF LLTPGAGTEE IIGGHEAKPH SRPYMAFVQF LQEKSRKRCG GILVRKDFVL 

        70         80         90        100        110        120 
TAAHCQGSSI NVTLGAHNIK EQERTQQFIP VKRPIPHPAY NPKNFSNDIM LLQLERKAKW 

       130        140        150        160        170        180 
TTAVRPLRLP SSKAQVKPGQ LCSVAGWGYV SMSTLATTLQ EVLLTVQKDC QCERLFHGNY 

       190        200        210        220        230        240 
SRATEICVGD PKKTQTGFKG DSGGPLVCKD VAQGILSYGN KKGTPPGVYI KVSHFLPWIK 


RTMKRL

« Hide

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References

« Hide 'large scale' references
[1]"Cloning of a gene that encodes a new member of the human cytotoxic cell protease family."
Meier M., Kwong P.C., Fregeau C.J., Atkinson E.A., Burrington M., Ehrman N., Sorensen O., Lin C.C., Wilkins J., Bleackley R.C.
Biochemistry 29:4042-4049(1990) [PubMed: 2193684] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Structure and evolutionary origin of the human granzyme H gene."
Haddad P., Jenne D.E., Tschopp J., Clement M.-V., Mathieu-Mahul D., Sasportes M.
Int. Immunol. 3:57-66(1991) [PubMed: 2049336] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Characterization of a novel, human cytotoxic lymphocyte-specific serine protease cDNA clone (CSP-C)."
Klein J.L., Selvakumar A., Trapani J.A., Dupont B.
Tissue Antigens 35:220-228(1990) [PubMed: 2402757] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J02907 Genomic DNA. Translation: AAA76859.1.
M57888 Genomic DNA. Translation: AAA03514.1.
M36118 mRNA. Translation: AAA03248.1.
M72150 Genomic DNA. Translation: AAA74885.1.
BC027974 mRNA. Translation: AAH27974.1.
IPIIPI00025400.
PIRA32692.
RefSeqNP_219491.1.
UniGeneHs.348264

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1LXVmodel-A21-246[»]
SMRP20718. Positions 21-245.
ModBaseSearch...

Protein-protein interaction databases

STRINGP20718.

Protein family/group databases

MEROPSS01.147.

Proteomic databases

PRIDEP20718.

Genome annotation databases

EnsemblENST00000216338; ENSP00000216338; ENSG00000100450; Homo sapiens. [Genome view]
GeneID2999.
KEGGhsa:2999.
UCSCuc001wpr.1. human.

Organism-specific databases

CTD2999.
GeneCardsGC14M024145.
H-InvDBHIX0011577.
HGNCHGNC:4710. GZMH.
MIM116831. gene.
PharmGKBPA29088.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG14107.
HOGENOMHBG755338.
HOVERGENP20718.
InParanoidP20718.
OMANYSRATE.
OrthoDBEOG9CZF11.
PhylomeDBP20718.

Gene expression databases

ArrayExpressP20718.
BgeeP20718.
CleanExHS_GZMH.
GenevestigatorP20718.
GermOnlineENSG00000100450. Homo sapiens.

Family and domain databases

InterProIPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
IPR009003. Ser/Cys_Pept_Trypsin-like.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio11892.
SOURCESearch...

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Entry information

Entry nameGRAH_HUMAN
AccessionPrimary (citable) accession number: P20718
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: January 19, 2010
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents