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P20715 (HMDH_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-hydroxy-3-methylglutaryl-coenzyme A reductase
Short name=HMG-CoA reductase
EC=1.1.1.34
Gene names
Name:hmgcr
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length883 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis.

Catalytic activity

(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.

Pathway

Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Induction

By estrogen; in male liver.

Sequence similarities

Belongs to the HMG-CoA reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8838833-hydroxy-3-methylglutaryl-coenzyme A reductase
PRO_0000114426

Regions

Transmembrane10 – 3930Helical; Potential
Transmembrane57 – 7822Helical; Potential
Transmembrane90 – 11425Helical; Potential
Transmembrane124 – 14926Helical; Potential
Transmembrane160 – 18728Helical; Potential
Transmembrane192 – 22029Helical; Potential
Transmembrane315 – 33925Helical; Potential
Region340 – 444105Linker
Region445 – 883439Catalytic

Sites

Active site5541Charge relay system By similarity
Active site6861Charge relay system By similarity
Active site7621Charge relay system By similarity
Active site8611Proton donor By similarity

Amino acid modifications

Glycosylation2821N-linked (GlcNAc...) Potential
Glycosylation5131N-linked (GlcNAc...) Potential
Glycosylation8651N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P20715 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 53D6D1D9294A9A1F

FASTA88396,720
        10         20         30         40         50         60 
MLSRLFRMHG QFVASHPWEV IVGTVTLTIC MMSMNMFTGN DKICGWNYAC PKFEEDVLSS 

        70         80         90        100        110        120 
DIIILTITRC IAILYIYFQF QNLRQLGSKY ILGIAGLFTI FSSFVFSTVV IHFLDKELTG 

       130        140        150        160        170        180 
LNEALPFFLL LIDLSKASAL AKFALSSNSQ DEVRDNIARG MAILGPTFTL EALVECLVIG 

       190        200        210        220        230        240 
VGTMSGVRQL EIMCCFGCMS VLANYFAFMT FFPACVSLVL ELSRESREGR PIWQLSQFAS 

       250        260        270        280        290        300 
VLEEEEDNKP NPVTQRVKMI MSLGLVLVHA HSRWISEPSS QNSTSISDHE VTTMLDDMMP 

       310        320        330        340        350        360 
KRVEPSMPLW QFYLSRMVTM DVEQIITLGL ALLLAVKYIF FEQTETESTF SMKNPIISPV 

       370        380        390        400        410        420 
AVQKKQIESC CRREPEQEKT VHVSTTEEAS SKEETEAVIK PLPLETSPKA KFIVGDSSPL 

       430        440        450        460        470        480 
ELSPEDKNTM FDLPEEPRPL DECVRILKNP DKGAQYLTDA EVISLVNAKH IPAYKLETMM 

       490        500        510        520        530        540 
ESPREGVAIR RQMLSDKLPQ RSALQSLPYK NYNYSLVMGA CCENVIGYMP IPVGVAGPLL 

       550        560        570        580        590        600 
LNNKEYQVPM ATTEGCLVAS TNRGCRAIML GGGAKSRVLA DGMTRGPVVR LPTACDAAEV 

       610        620        630        640        650        660 
KAWLDSAEGF KVIKDAFDST SRFARLGRLQ NCVAGRNLYI RFQSKTGDAM GMNMISKVTE 

       670        680        690        700        710        720 
QALARLQEEF PDLHVLAVSG NYCTDKKPAA INWIEGRGKS VVCEAIIPAK VVREVLKSST 

       730        740        750        760        770        780 
EALVEVNINK NFIGSAMAGS IGGYNAHAAN IVTAIYIACG QDAAQNVGSS NCITIMEATG 

       790        800        810        820        830        840 
PTYEDLYISC TMPSIEIGTV GGGTNLAPQQ ACLQMLGVQG ASTETPGKNA CQLAQIVCST 

       850        860        870        880 
VMAGELSLMA ALAAGHLVKS HMVHNRSKIN LQDLPGTCTK KAA

« Hide

References

[1]"Nucleotide sequence and estrogen induction of Xenopus laevis 3-hydroxy-3-methylglutaryl-coenzyme A reductase."
Chen H., Shapiro D.J.
J. Biol. Chem. 265:4622-4629(1990) [PubMed: 2307680] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M29258 mRNA. Translation: AAA49740.1.
PIRA35728.
RefSeqNP_001081280.1. NM_001087811.1.
UniGeneXl.800.

3D structure databases

ProteinModelPortalP20715.
SMRP20715. Positions 436-866.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID397750.
KEGGxla:397750.

Organism-specific databases

CTD3156.
XenbaseXB-GENE-5840728. hmgcr.

Phylogenomic databases

HOVERGENHBG000453.

Family and domain databases

InterProIPR002202. HMG_CoA_Rdtase.
IPR023074. HMG_CoA_Rdtase_cat.
IPR023076. HMG_CoA_Rdtase_CS.
IPR004554. HMG_CoA_Rdtase_eu_arc.
IPR004816. HMG_CoA_Rdtase_metazoan.
IPR023282. HMG_CoA_Rdtase_N.
IPR009023. HMG_CoA_Rdtase_NAD(P)-bd.
IPR009029. HMG_CoA_Rdtase_sub-bd.
IPR000731. SSD.
[Graphical view]
Gene3DG3DSA:3.30.70.420. G3DSA:3.30.70.420. 1 hit.
G3DSA:3.90.770.10. HMG-CoA_red. 2 hits.
G3DSA:1.10.3270.10. HMG_CoA_Rdtase_N. 1 hit.
KOK00021.
PANTHERPTHR10572. HMG-CoA_red. 1 hit.
PfamPF00368. HMG-CoA_red. 1 hit.
[Graphical view]
PRINTSPR00071. HMGCOARDTASE.
SUPFAMSSF55035. HMG_CoA_NAD_bind. 1 hit.
SSF56542. HMG_CoA_sub_bind. 1 hit.
TIGRFAMsTIGR00920. 2A060605. 1 hit.
TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITEPS00066. HMG_COA_REDUCTASE_1. 1 hit.
PS00318. HMG_COA_REDUCTASE_2. 1 hit.
PS01192. HMG_COA_REDUCTASE_3. 1 hit.
PS50065. HMG_COA_REDUCTASE_4. 1 hit.
PS50156. SSD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHMDH_XENLA
AccessionPrimary (citable) accession number: P20715
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 16, 2011
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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