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Protein

ATP-dependent DNA helicase uvsW

Gene

uvsW

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays important roles in recombination-dependent DNA repair and the reorganization of stalled replication forks during viral DNA synthesis.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi135 – 1428ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase uvsW (EC:3.6.4.12)
Alternative name(s):
Dar protein
Gene namesi
Name:uvsW
Synonyms:dar
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi141 – 1411K → R: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 587587ATP-dependent DNA helicase uvsWPRO_0000164988Add
BLAST

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with gp32.1 Publication

Structurei

Secondary structure

1
587
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Turni9 – 113Combined sources
Beta strandi12 – 165Combined sources
Helixi19 – 2810Combined sources
Beta strandi30 – 323Combined sources
Turni34 – 374Combined sources
Helixi40 – 434Combined sources
Beta strandi49 – 546Combined sources
Beta strandi59 – 613Combined sources
Helixi62 – 676Combined sources
Helixi68 – 747Combined sources
Beta strandi79 – 813Combined sources
Helixi83 – 864Combined sources
Helixi93 – 1019Combined sources
Beta strandi105 – 1073Combined sources
Beta strandi110 – 1123Combined sources
Helixi116 – 12813Combined sources
Beta strandi129 – 1335Combined sources
Turni138 – 1403Combined sources
Helixi141 – 15515Combined sources
Beta strandi157 – 1637Combined sources
Helixi167 – 17913Combined sources
Helixi185 – 1873Combined sources
Beta strandi188 – 1903Combined sources
Helixi192 – 1943Combined sources
Helixi199 – 2013Combined sources
Beta strandi206 – 2105Combined sources
Helixi212 – 2154Combined sources
Helixi220 – 2256Combined sources
Beta strandi226 – 2327Combined sources
Helixi234 – 2363Combined sources
Helixi239 – 2457Combined sources
Turni246 – 2483Combined sources
Beta strandi254 – 2585Combined sources
Helixi262 – 2643Combined sources
Helixi269 – 2768Combined sources
Beta strandi278 – 2803Combined sources
Beta strandi298 – 3058Combined sources
Helixi308 – 3147Combined sources
Helixi319 – 3279Combined sources
Helixi330 – 34415Combined sources
Turni345 – 3473Combined sources
Beta strandi349 – 3568Combined sources
Helixi357 – 36812Combined sources
Beta strandi372 – 3809Combined sources
Helixi383 – 39513Combined sources
Beta strandi400 – 4045Combined sources
Helixi405 – 4106Combined sources
Beta strandi416 – 4249Combined sources
Helixi431 – 44010Combined sources
Beta strandi450 – 4578Combined sources
Beta strandi463 – 4664Combined sources
Helixi476 – 49015Combined sources
Beta strandi495 – 5017Combined sources
Beta strandi514 – 5174Combined sources
Helixi518 – 5236Combined sources
Helixi528 – 5336Combined sources
Helixi538 – 55114Combined sources
Turni552 – 5543Combined sources
Helixi560 – 57819Combined sources
Turni583 – 5853Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RIFX-ray2.00A/B1-282[»]
2JPNNMR-A512-587[»]
2OCAX-ray2.70A1-502[»]
ProteinModelPortaliP20703.
SMRiP20703. Positions 1-503, 512-587.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20703.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini122 – 280159Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini335 – 501167Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK18959.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR020975. DNA-helicase_ATP-dep_UvsW.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF11637. UvsW. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20703-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIKVHFHDF SHVRIDCEES TFHELRDFFS FEADGYRFNP RFRYGNWDGR
60 70 80 90 100
IRLLDYNRLL PFGLVGQIKK FCDNFGYKAW IDPQINEKEE LSRKDFDEWL
110 120 130 140 150
SKLEIYSGNK RIEPHWYQKD AVFEGLVNRR RILNLPTSAG KSLIQALLAR
160 170 180 190 200
YYLENYEGKI LIIVPTTALT TQMADDFVDY RLFSHAMIKK IGGGASKDDK
210 220 230 240 250
YKNDAPVVVG TWQTVVKQPK EWFSQFGMMM NDECHLATGK SISSIISGLN
260 270 280 290 300
NCMFKFGLSG SLRDGKANIM QYVGMFGEIF KPVTTSKLME DGQVTELKIN
310 320 330 340 350
SIFLRYPDEF TTKLKGKTYQ EEIKIITGLS KRNKWIAKLA IKLAQKDENA
360 370 380 390 400
FVMFKHVSHG KAIFDLIKNE YDKVYYVSGE VDTETRNIMK TLAENGKGII
410 420 430 440 450
IVASYGVFST GISVKNLHHV VLAHGVKSKI IVLQTIGRVL RKHGSKTIAT
460 470 480 490 500
VWDLIDSAGV KPKSANTKKK YVHLNYLLKH GIDRIQRYAD EKFNYVMKTV
510 520 530 540 550
NLISFGPLEK KMLLEFKQFL YEASIDEFMG KIASCQTLEG LEELEAYYKK
560 570 580
RVKETELKDT DDISVRDALA GKRAELEDSD DEVEESF
Length:587
Mass (Da):67,525
Last modified:February 1, 1995 - v2
Checksum:iD7FBD8CE1E38C7CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF158101 Genomic DNA. Translation: AAD42668.1.
X56097 Genomic DNA. Translation: CAA39576.1.
X14869 Genomic DNA. Translation: CAA33014.1.
PIRiPQ0097. S10498.
RefSeqiNP_049796.1. NC_000866.4.

Genome annotation databases

GeneIDi1258733.
KEGGivg:1258733.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF158101 Genomic DNA. Translation: AAD42668.1.
X56097 Genomic DNA. Translation: CAA39576.1.
X14869 Genomic DNA. Translation: CAA33014.1.
PIRiPQ0097. S10498.
RefSeqiNP_049796.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RIFX-ray2.00A/B1-282[»]
2JPNNMR-A512-587[»]
2OCAX-ray2.70A1-502[»]
ProteinModelPortaliP20703.
SMRiP20703. Positions 1-503, 512-587.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258733.
KEGGivg:1258733.

Phylogenomic databases

KOiK18959.

Miscellaneous databases

EvolutionaryTraceiP20703.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR020975. DNA-helicase_ATP-dep_UvsW.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF11637. UvsW. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Bacteriophage T4 UvsW protein is a helicase involved in recombination, repair and the regulation of DNA replication origins."
    Carles-Kinch K., George J.W., Kreuzer K.N.
    EMBO J. 16:4142-4151(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS, CHARACTERIZATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The nucleotide sequence of the region of bacteriophage T4 inh(lip)-hoc genes."
    Kaliman A.V., Khasanova M.A., Kryukov V.M., Tanyashin V.I., Bayev A.A.
    Nucleic Acids Res. 18:4277-4277(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-139.
  4. "Expression and function of the uvsW gene of bacteriophage T4."
    Derr L.K., Kreuzer K.N.
    J. Mol. Biol. 214:643-656(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15.
  5. "RecG and UvsW catalyse robust DNA rewinding critical for stalled DNA replication fork rescue."
    Manosas M., Perumal S.K., Bianco P., Ritort F., Benkovic S.J., Croquette V.
    Nat. Commun. 4:2368-2368(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Interaction of T4 UvsW helicase and single-stranded DNA binding protein gp32 through its carboxy-terminal acidic tail."
    Perumal S.K., Nelson S.W., Benkovic S.J.
    J. Mol. Biol. 425:2823-2839(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GP32.
  7. "The crystal structure of the UvsW helicase from bacteriophage T4."
    Sickmier E.A., Kreuzer K.N., White S.W.
    Structure 12:583-592(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-282.

Entry informationi

Entry nameiUVSW_BPT4
AccessioniPrimary (citable) accession number: P20703
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1995
Last modified: October 14, 2015
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.