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Protein

ATP-dependent DNA helicase uvsW

Gene

uvsW

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays important roles in recombination-dependent DNA repair and the reorganization of stalled replication forks during viral DNA synthesis.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi135 – 142ATPPROSITE-ProRule annotation8

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase uvsW (EC:3.6.4.12)
Alternative name(s):
Dar protein
Gene namesi
Name:uvsW
Synonyms:dar
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi141K → R: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001649881 – 587ATP-dependent DNA helicase uvsWAdd BLAST587

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with gp32.1 Publication

Structurei

Secondary structure

1587
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Turni9 – 11Combined sources3
Beta strandi12 – 16Combined sources5
Helixi19 – 28Combined sources10
Beta strandi30 – 32Combined sources3
Turni34 – 37Combined sources4
Helixi40 – 43Combined sources4
Beta strandi49 – 54Combined sources6
Beta strandi59 – 61Combined sources3
Helixi62 – 67Combined sources6
Helixi68 – 74Combined sources7
Beta strandi79 – 81Combined sources3
Helixi83 – 86Combined sources4
Helixi93 – 101Combined sources9
Beta strandi105 – 107Combined sources3
Beta strandi110 – 112Combined sources3
Helixi116 – 128Combined sources13
Beta strandi129 – 133Combined sources5
Turni138 – 140Combined sources3
Helixi141 – 155Combined sources15
Beta strandi157 – 163Combined sources7
Helixi167 – 179Combined sources13
Helixi185 – 187Combined sources3
Beta strandi188 – 190Combined sources3
Helixi192 – 194Combined sources3
Helixi199 – 201Combined sources3
Beta strandi206 – 210Combined sources5
Helixi212 – 215Combined sources4
Helixi220 – 225Combined sources6
Beta strandi226 – 232Combined sources7
Helixi234 – 236Combined sources3
Helixi239 – 245Combined sources7
Turni246 – 248Combined sources3
Beta strandi254 – 258Combined sources5
Helixi262 – 264Combined sources3
Helixi269 – 276Combined sources8
Beta strandi278 – 280Combined sources3
Beta strandi298 – 305Combined sources8
Helixi308 – 314Combined sources7
Helixi319 – 327Combined sources9
Helixi330 – 344Combined sources15
Turni345 – 347Combined sources3
Beta strandi349 – 356Combined sources8
Helixi357 – 368Combined sources12
Beta strandi372 – 380Combined sources9
Helixi383 – 395Combined sources13
Beta strandi400 – 404Combined sources5
Helixi405 – 410Combined sources6
Beta strandi416 – 424Combined sources9
Helixi431 – 440Combined sources10
Beta strandi450 – 457Combined sources8
Beta strandi463 – 466Combined sources4
Helixi476 – 490Combined sources15
Beta strandi495 – 501Combined sources7
Beta strandi514 – 517Combined sources4
Helixi518 – 523Combined sources6
Helixi528 – 533Combined sources6
Helixi538 – 551Combined sources14
Turni552 – 554Combined sources3
Helixi560 – 578Combined sources19
Turni583 – 585Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RIFX-ray2.00A/B1-282[»]
2JPNNMR-A512-587[»]
2OCAX-ray2.70A1-502[»]
ProteinModelPortaliP20703.
SMRiP20703.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20703.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini122 – 280Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST159
Domaini335 – 501Helicase C-terminalPROSITE-ProRule annotationAdd BLAST167

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK18959.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR020975. DNA-helicase_ATP-dep_UvsW.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF11637. UvsW. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20703-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIKVHFHDF SHVRIDCEES TFHELRDFFS FEADGYRFNP RFRYGNWDGR
60 70 80 90 100
IRLLDYNRLL PFGLVGQIKK FCDNFGYKAW IDPQINEKEE LSRKDFDEWL
110 120 130 140 150
SKLEIYSGNK RIEPHWYQKD AVFEGLVNRR RILNLPTSAG KSLIQALLAR
160 170 180 190 200
YYLENYEGKI LIIVPTTALT TQMADDFVDY RLFSHAMIKK IGGGASKDDK
210 220 230 240 250
YKNDAPVVVG TWQTVVKQPK EWFSQFGMMM NDECHLATGK SISSIISGLN
260 270 280 290 300
NCMFKFGLSG SLRDGKANIM QYVGMFGEIF KPVTTSKLME DGQVTELKIN
310 320 330 340 350
SIFLRYPDEF TTKLKGKTYQ EEIKIITGLS KRNKWIAKLA IKLAQKDENA
360 370 380 390 400
FVMFKHVSHG KAIFDLIKNE YDKVYYVSGE VDTETRNIMK TLAENGKGII
410 420 430 440 450
IVASYGVFST GISVKNLHHV VLAHGVKSKI IVLQTIGRVL RKHGSKTIAT
460 470 480 490 500
VWDLIDSAGV KPKSANTKKK YVHLNYLLKH GIDRIQRYAD EKFNYVMKTV
510 520 530 540 550
NLISFGPLEK KMLLEFKQFL YEASIDEFMG KIASCQTLEG LEELEAYYKK
560 570 580
RVKETELKDT DDISVRDALA GKRAELEDSD DEVEESF
Length:587
Mass (Da):67,525
Last modified:February 1, 1995 - v2
Checksum:iD7FBD8CE1E38C7CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF158101 Genomic DNA. Translation: AAD42668.1.
X56097 Genomic DNA. Translation: CAA39576.1.
X14869 Genomic DNA. Translation: CAA33014.1.
PIRiPQ0097. S10498.
RefSeqiNP_049796.1. NC_000866.4.

Genome annotation databases

GeneIDi1258733.
KEGGivg:1258733.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF158101 Genomic DNA. Translation: AAD42668.1.
X56097 Genomic DNA. Translation: CAA39576.1.
X14869 Genomic DNA. Translation: CAA33014.1.
PIRiPQ0097. S10498.
RefSeqiNP_049796.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RIFX-ray2.00A/B1-282[»]
2JPNNMR-A512-587[»]
2OCAX-ray2.70A1-502[»]
ProteinModelPortaliP20703.
SMRiP20703.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258733.
KEGGivg:1258733.

Phylogenomic databases

KOiK18959.

Miscellaneous databases

EvolutionaryTraceiP20703.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR020975. DNA-helicase_ATP-dep_UvsW.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF11637. UvsW. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUVSW_BPT4
AccessioniPrimary (citable) accession number: P20703
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.