P20702 (ITAX_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Integrin alpha-X

Alternative name(s):
CD11 antigen-like family member C
Leu M5
Leukocyte adhesion glycoprotein p150,95 alpha chain
Leukocyte adhesion receptor p150,95
CD_antigen=CD11c

Gene names

Name:	ITGAX
Synonyms:	CD11C

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1163 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Subunit structure	Heterodimer of an alpha and a beta subunit. Alpha-X associates with beta-2.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Predominantly expressed in monocytes and granulocytes.
Domain	The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Sequence similarities	Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium Magnesium
Molecular function	Integrin Receptor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	blood coagulation Traceable author statement. Source: Reactome cell adhesion Traceable author statement. Source: ProtInc integrin-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW leukocyte migration Traceable author statement. Source: Reactome organ morphogenesis Traceable author statement. Source: ProtInc
Cellular component	integrin complex Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: BHF-UCL receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Ref.5

Chain

20 – 1163

1144

Integrin alpha-X

PRO_0000016294

Regions

Topological domain

20 – 1107

1088

Extracellular Potential

Transmembrane

1108 – 1128

21

Helical; Potential

Topological domain

1129 – 1163

35

Cytoplasmic Potential

Repeat

23 – 78

56

FG-GAP 1

Repeat

79 – 138

60

FG-GAP 2

Domain

165 – 339

175

VWFA

Repeat

340 – 391

52

FG-GAP 3

Repeat

392 – 445

54

FG-GAP 4

Repeat

446 – 504

59

FG-GAP 5

Repeat

507 – 565

59

FG-GAP 6

Repeat

570 – 630

61

FG-GAP 7

Calcium binding

466 – 474

9

Potential

Calcium binding

530 – 538

9

Potential

Calcium binding

593 – 601

9

Potential

Motif

1131 – 1135

5

GFFKR motif

Amino acid modifications

Glycosylation

61

1

N-linked (GlcNAc...) Potential

Glycosylation

89

1

N-linked (GlcNAc...) Potential

Glycosylation

392

1

N-linked (GlcNAc...) Potential

Glycosylation

697

1

N-linked (GlcNAc...) Potential

Glycosylation

735

1

N-linked (GlcNAc...) Potential

Glycosylation

899

1

N-linked (GlcNAc...) Ref.6

Glycosylation

939

1

N-linked (GlcNAc...) Potential

Glycosylation

1050

1

N-linked (GlcNAc...) Potential

Disulfide bond

69 ↔ 76

By similarity

Disulfide bond

108 ↔ 126

By similarity

Disulfide bond

655 ↔ 712

By similarity

Disulfide bond

771 ↔ 777

By similarity

Disulfide bond

848 ↔ 863

By similarity

Disulfide bond

998 ↔ 1022

By similarity

Disulfide bond

1027 ↔ 1032

By similarity

Natural variations

Natural variant

48

1

W → R.
Corresponds to variant rs2230424 [ dbSNP | Ensembl ].

VAR_018672

Natural variant

201

1

F → L. Ref.2
Corresponds to variant rs1574566 [ dbSNP | Ensembl ].

VAR_049632

Natural variant

251

1

A → T. Ref.1 Ref.2
Corresponds to variant rs2230428 [ dbSNP | Ensembl ].

VAR_031925

Natural variant

517

1

P → R.
Corresponds to variant rs2230429 [ dbSNP | Ensembl ].

VAR_031926

Natural variant

547

1

E → K. Ref.4
Corresponds to variant rs17853815 [ dbSNP | Ensembl ].

VAR_031927

Natural variant

564

1

I → V.
Corresponds to variant rs2230430 [ dbSNP | Ensembl ].

VAR_059363

Natural variant

971

1

F → L.
Corresponds to variant rs2230427 [ dbSNP | Ensembl ].

VAR_031928

Natural variant

1012

1

A → V. Ref.7

VAR_066662

Experimental info

Sequence conflict

209

1

S → T in AAA59180. Ref.1

Sequence conflict

209

1

S → T in AAA51620. Ref.2

Sequence conflict

266

1

S → T in AAA51620. Ref.2

Sequence conflict

327

1

N → T in AAA51620. Ref.2

Sequence conflict

330

1

K → R in AAA51620. Ref.2

Sequence conflict

335

1

A → P in AAA51620. Ref.2

Sequence conflict

460

1

A → P in AAA51620. Ref.2

Sequence conflict

469

1

S → T in AAA59180. Ref.1

Sequence conflict

480

1

A → P in AAA51620. Ref.2

Sequence conflict

490

1

G → A in AAA51620. Ref.2

Sequence conflict

756

1

D → L in AAA59180. Ref.1

Sequence conflict

819

1

I → V in AAH38237. Ref.4

Sequence conflict

990

1

H → L in AAA51620. Ref.2

Sequence conflict

1005

1

P → G in AAA51620. Ref.2

Sequence conflict

1161 – 1163

3

SEK → TPHYPQDNV in AAH38237. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1163

Helix Strand Turn

Details...

Beta strand

150 – 157

8

Helix

164 – 178

15

Turn

183 – 185

3

Beta strand

186 – 201

16

Helix

203 – 208

6

Helix

212 – 216

5

Helix

228 – 236

9

Turn

237 – 240

4

Helix

242 – 244

3

Beta strand

250 – 260

11

Helix

269 – 278

10

Beta strand

282 – 289

8

Helix

290 – 293

4

Helix

298 – 304

7

Beta strand

307 – 309

3

Helix

310 – 312

3

Beta strand

313 – 318

6

Helix

319 – 325

7

Helix

326 – 334

9

Sequences

	Sequence		Length	Mass (Da)
	P20702 [UniParc]. Last modified May 1, 2007. Version 3. Checksum: 8947288C43E76BE2	FASTA	1,163	127,829
	10 20 30 40 50 60 MTRTRAALLL FTALATSLGF NLDTEELTAF RVDSAGFGDS VVQYANSWVV VGAPQKITAA 70 80 90 100 110 120 NQTGGLYQCG YSTGACEPIG LQVPPEAVNM SLGLSLASTT SPSQLLACGP TVHHECGRNM 130 140 150 160 170 180 YLTGLCFLLG PTQLTQRLPV SRQECPRQEQ DIVFLIDGSG SISSRNFATM MNFVRAVISQ 190 200 210 220 230 240 FQRPSTQFSL MQFSNKFQTH FTFEEFRRSS NPLSLLASVH QLQGFTYTAT AIQNVVHRLF 250 260 270 280 290 300 HASYGARRDA AKILIVITDG KKEGDSLDYK DVIPMADAAG IIRYAIGVGL AFQNRNSWKE 310 320 330 340 350 360 LNDIASKPSQ EHIFKVEDFD ALKDIQNQLK EKIFAIEGTE TTSSSSFELE MAQEGFSAVF 370 380 390 400 410 420 TPDGPVLGAV GSFTWSGGAF LYPPNMSPTF INMSQENVDM RDSYLGYSTE LALWKGVQSL 430 440 450 460 470 480 VLGAPRYQHT GKAVIFTQVS RQWRMKAEVT GTQIGSYFGA SLCSVDVDSD GSTDLVLIGA 490 500 510 520 530 540 PHYYEQTRGG QVSVCPLPRG WRRWWCDAVL YGEQGHPWGR FGAALTVLGD VNGDKLTDVV 550 560 570 580 590 600 IGAPGEEENR GAVYLFHGVL GPSISPSHSQ RIAGSQLSSR LQYFGQALSG GQDLTQDGLV 610 620 630 640 650 660 DLAVGARGQV LLLRTRPVLW VGVSMQFIPA EIPRSAFECR EQVVSEQTLV QSNICLYIDK 670 680 690 700 710 720 RSKNLLGSRD LQSSVTLDLA LDPGRLSPRA TFQETKNRSL SRVRVLGLKA HCENFNLLLP 730 740 750 760 770 780 SCVEDSVTPI TLRLNFTLVG KPLLAFRNLR PMLAADAQRY FTASLPFEKN CGADHICQDN 790 800 810 820 830 840 LGISFSFPGL KSLLVGSNLE LNAEVMVWND GEDSYGTTIT FSHPAGLSYR YVAEGQKQGQ 850 860 870 880 890 900 LRSLHLTCDS APVGSQGTWS TSCRINHLIF RGGAQITFLA TFDVSPKAVL GDRLLLTANV 910 920 930 940 950 960 SSENNTPRTS KTTFQLELPV KYAVYTVVSS HEQFTKYLNF SESEEKESHV AMHRYQVNNL 970 980 990 1000 1010 1020 GQRDLPVSIN FWVPVELNQE AVWMDVEVSH PQNPSLRCSS EKIAPPASDF LAHIQKNPVL 1030 1040 1050 1060 1070 1080 DCSIAGCLRF RCDVPSFSVQ EELDFTLKGN LSFGWVRQIL QKKVSVVSVA EITFDTSVYS 1090 1100 1110 1120 1130 1140 QLPGQEAFMR AQTTTVLEKY KVHNPTPLIV GSSIGGLLLL ALITAVLYKV GFFKRQYKEM 1150 1160 MEEANGQIAP ENGTQTPSPP SEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and complete primary structure of the alpha subunit of a leukocyte adhesion glycoprotein, p150,95." Corbi A.L., Miller L.J., O'Connor K., Larson R.S., Springer T.A. EMBO J. 6:4023-4028(1987) [PubMed: 3327687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-251.
[2]	"Genomic structure of an integrin alpha subunit, the leukocyte p150,95 molecule." Corbi A.L., Garcia-Aguilar J., Springer T.A. J. Biol. Chem. 265:2782-2788(1990) [PubMed: 2303426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-201 AND THR-251.
[3]	Erratum Corbi A.L., Garcia-Aguilar J., Springer T.A. J. Biol. Chem. 265:12750-12751(1990)
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LYS-547. Tissue: Blood.
[5]	"Purification and alpha subunit N-terminal sequences of human Mac-1 and p150,95 leukocyte adhesion proteins." Miller L.J., Wiebe M., Springer T.A. J. Immunol. 138:2381-2383(1987) [PubMed: 3549901] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-43.
[6]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-899, MASS SPECTROMETRY. Tissue: Liver.
[7]	"VPS35 mutations in Parkinson disease." Vilarino-Guell C., Wider C., Ross O.A., Dachsel J.C., Kachergus J.M., Lincoln S.J., Soto-Ortolaza A.I., Cobb S.A., Wilhoite G.J., Bacon J.A., Behrouz B., Melrose H.L., Hentati E., Puschmann A., Evans D.M., Conibear E., Wasserman W.W., Aasly J.O. , Burkhard P.R., Djaldetti R., Ghika J., Hentati F., Krygowska-Wajs A., Lynch T., Melamed E., Rajput A., Rajput A.H., Solida A., Wu R.M., Uitti R.J., Wszolek Z.K., Vingerhoets F., Farrer M.J. Am. J. Hum. Genet. 89:162-167(2011) [PubMed: 21763482] [Abstract] Cited for: VARIANT VAL-1012.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M81695 mRNA. Translation: AAA59180.1.
M29165 mRNA. No translation available.
M29487 , M29482, M29483, M29484, M29485, M29486 Genomic DNA. Translation: AAA51620.1. Sequence problems.
BC038237 mRNA. Translation: AAH38237.1.

IPI

IPI00479724.

PIR

RWHU1C. A36584.

RefSeq

NP_000878.2. NM_000887.3.

UniGene

Hs.248472.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1N3Y	X-ray	1.65	A	147-338	[»]
3K6S	X-ray	3.50	A/C/E/G	20-1103	[»]
3K71	X-ray	3.95	A/C/E/G	20-1103	[»]
3K72	X-ray	3.70	A/C	20-1103	[»]

ProteinModelPortal

P20702.

SMR

P20702. Positions 20-1138.

ModBase

Search...

Protein-protein interaction databases

IntAct

P20702. 1 interaction.

STRING

P20702.

Polymorphism databases

DMDM

146345441.

Proteomic databases

PRIDE

P20702.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000268296; ENSP00000268296; ENSG00000140678.

GeneID

3687.

KEGG

hsa:3687.

UCSC

uc002ebu.1. human.

Organism-specific databases

CTD

3687.

GeneCards

GC16P031366.

H-InvDB

HIX0012988.

HGNC

HGNC:6152. ITGAX.

HPA

CAB004458.
HPA004723.

MIM

151510. gene.

neXtProt

NX_P20702.

PharmGKB

PA29952.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00600000084082.

HOGENOM

HBG443936.

HOVERGEN

HBG100530.

InParanoid

P20702.

OMA

VMSQFQR.

OrthoDB

EOG4TQM86.

PhylomeDB

P20702.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.
REACT_604. Hemostasis.

Gene expression databases

ArrayExpress

P20702.

Bgee

P20702.

CleanEx

HS_ITGAX.

Genevestigator

P20702.

GermOnline

ENSG00000140678. Homo sapiens.

Family and domain databases

InterPro

IPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR002035. VWF_A.
[Graphical view]

KO

K06462.

Pfam

PF01839. FG-GAP. 1 hit.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]

PRINTS

PR01185. INTEGRINA.

SMART

SM00191. Int_alpha. 5 hits.
SM00327. VWA. 1 hit.
[Graphical view]

PROSITE

PS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

14429.

SOURCE

Search...

Entry information

Entry name

ITAX_HUMAN

Accession

Primary (citable) accession number: P20702
Secondary accession number(s): Q8IVA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	May 1, 2007
Last modified:	January 25, 2012
This is version 126 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.