P20698 (PRO28_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prolamin PPROL 17D

Gene names

Ordered Locus Names:

Os12g0269200, LOC_Os12g16890

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Seed storage protein; serves as a source of nitrogen, carbon and sulfur for the young developing seedling.
Subcellular location	Vacuole › aleurone grain. Note: In rice, prolamin accumulates as a type I protein body which originates directly from the endoplasmic reticulum.
Miscellaneous	Lacks significant tandem repetitive sequences.
Sequence similarities	Belongs to the prolamin family.
Sequence caution	The sequence AAA50423.1 differs from that shown. Reason: Frameshift at several positions. The sequence AAA50423.1 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
Cellular component	Vacuole
Domain	Signal
Molecular function	Seed storage protein Storage protein
PTM	Pyrrolidone carboxylic acid
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	aleurone grain Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 156	137	Prolamin PPROL 17D		PRO_0000032266
Amino acid modifications
	Modified residue	20	1	Pyrrolidone carboxylic acid Potential

Sequences

	Sequence		Length	Mass (Da)
	P20698 [UniParc]. Last modified June 13, 2006. Version 2. Checksum: 1CF73D0C15D9000B	FASTA	156	17,562
	10 20 30 40 50 60 MKIIFFFALL AIAACSASAQ FDAVTQVYRQ YQLQPHLMLQ QQMLSPCGEF VRQQCSTVAT 70 80 90 100 110 120 PFFQSPVFQL RNCQVMQQQC CQQLRMIAQQ SHCQAISSVQ AIVQQLRLQQ FASVYFDQSQ 130 140 150 AQAQAMLALN MPSICGIYPS YNTAPCSIPT VGGIWY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, expression, and heterogeneity of the rice seed prolamines." Kim W.T., Okita T.W. Plant Physiol. 88:649-655(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. M201. Tissue: Seed.
[2]	"Amino acid sequencing and cDNA cloning of major rice seed storage proteins, the 13 kDa prolamins, extrtacted from PB-I protein bodies." Mitsukawa N., Konishi R., Kidzu K., Ohtsuki K., Masumura T., Tanaka K. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Small cysteine-rich peptides resembling antimicrobial peptides have been under-predicted in plants." Silverstein K.A.T., Moskal W.A. Jr., Wu H.C., Underwood B.A., Graham M.A., Town C.D., VandenBosch K.A. Plant J. 51:262-280(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M23745 mRNA. Translation: AAA50423.1. Sequence problems. AB016505 mRNA. Translation: BAA36699.1. DP000011 Genomic DNA. Translation: ABA97054.1.
PIR	JA0167.
RefSeq	NP_001066544.1. NM_001073076.1.
UniGene	Os.13715.
3D structure databases
ProteinModelPortal	P20698.
ModBase	Search...
Protein-protein interaction databases
IntAct	P20698. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os12g16890.1; LOC_Os12g16890.1; LOC_Os12g16890.
GeneID	4351949.
KEGG	osa:4351949.
Organism-specific databases
Gramene	P20698.
Phylogenomic databases
eggNOG	NOG304409.
HOGENOM	HOG000230499.
ProtClustDB	CLSN2695517.
Family and domain databases
Gene3D	1.10.120.10. 1 hit.
InterPro	IPR016140. Bifunc_inhib/LTP/seed_store. IPR001954. Glia_glutenin. IPR013771. Trypsin/amylase_inhib. [Graphical view]
Pfam	PF00234. Tryp_alpha_amyl. 1 hit. [Graphical view]
PRINTS	PR00208. GLIADGLUTEN.
SMART	SM00499. AAI. 1 hit. [Graphical view]
SUPFAM	SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRO28_ORYSJ

Accession

Primary (citable) accession number: P20698
Secondary accession number(s): Q2QUA8, Q9ZWJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	June 13, 2006
Last modified:	March 6, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program