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Protein

Myosin light chain kinase 2, skeletal/cardiac muscle

Gene

Mylk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in the level of global muscle contraction and cardiac function (By similarity). Phosphorylates a specific serine in the N-terminus of a myosin light chain.By similarity

Catalytic activityi

ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei328 – 3281ATPPROSITE-ProRule annotation
Active sitei420 – 4201Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi305 – 3139ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • myosin light chain binding Source: RGD
  • myosin light chain kinase activity Source: RGD

GO - Biological processi

  • muscle contraction Source: RGD
  • peptidyl-serine phosphorylation Source: RGD
  • positive regulation of fast-twitch skeletal muscle fiber contraction Source: RGD
  • protein phosphorylation Source: RGD
  • regulation of cell cycle Source: RGD
  • regulation of MAPK cascade Source: RGD
  • regulation of muscle contraction Source: RGD
  • regulation of neuronal synaptic plasticity Source: RGD
  • synaptic vesicle transport Source: RGD
  • water transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.18. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain kinase 2, skeletal/cardiac muscle (EC:2.7.11.18)
Short name:
MLCK2
Gene namesi
Name:Mylk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620934. Mylk2.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.By similarity

GO - Cellular componenti

  • cytosol Source: GOC
  • dendrite Source: RGD
  • dendritic spine Source: RGD
  • neuronal cell body Source: RGD
  • postsynaptic density Source: RGD
  • synaptic vesicle Source: RGD
  • terminal bouton Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 610609Myosin light chain kinase 2, skeletal/cardiac musclePRO_0000086411Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei160 – 1601PhosphoserineBy similarity
Modified residuei166 – 1661PhosphoserineBy similarity
Modified residuei168 – 1681PhosphoserineBy similarity
Modified residuei459 – 4591PhosphothreonineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP20689.
PRIDEiP20689.

PTM databases

iPTMnetiP20689.
PhosphoSiteiP20689.

Interactioni

Subunit structurei

May interact with centrin.By similarity

GO - Molecular functioni

  • myosin light chain binding Source: RGD

Protein-protein interaction databases

IntActiP20689. 2 interactions.
MINTiMINT-1346460.
STRINGi10116.ENSRNOP00000011255.

Structurei

3D structure databases

ProteinModelPortaliP20689.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini299 – 554256Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni588 – 60013Calmodulin-bindingBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi95 – 1028Poly-Gly
Compositional biasi275 – 2828Poly-Pro

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233016.
HOVERGENiHBG080416.
InParanoidiP20689.
KOiK00907.
PhylomeDBiP20689.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20689-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATENGAVEL GTQSLSTDHP PTDAAGDGSP ASEKEPSLPD TEKDLGPTNT
60 70 80 90 100
KKDPGAPDPK KNPDPPSLKK TPEAPGPEKK GDSAPASASN QGPSGEGDGG
110 120 130 140 150
GGPAEGGTGP PAVLPQPTAT ADASIQKLDA TQAPSGNQES GEAKAGKKAA
160 170 180 190 200
ECREAGRRGS PAFLHSPSCP AIISCSEKTL AMKPLSETTE LIFAGVSETP
210 220 230 240 250
DPQDPGPAKD EGGTNTLADG KEEAEAGQAE QAKVQGDTSQ RIGFQAVPSE
260 270 280 290 300
RAEVGQALCL TAKEEDCFQI LDDCPPPPAP FPHRIVELRT GNVSSEFSMN
310 320 330 340 350
SKEALGGGKF GAVCTCTERS TGLKLAAKVI KKQTPKDKEM VLLEIEVMNQ
360 370 380 390 400
LNHRNLIQLY SAIETSHEII LFMEYIEGGE LFERIVDEDY QLTEVDTMVF
410 420 430 440 450
VRQICDGILF MHKMRVLHLD LKPENILCVN TTGHLVKIID FGLARRYNPN
460 470 480 490 500
EKLKVNFGTP EFLSPEVVNY DQISDKTDMW SLGVITYMLL SGLSPFLGDD
510 520 530 540 550
DTETLNNVLS ANWYFDEETF EAVSDEAKDF VSNLITKDQS ARMSAEQCLA
560 570 580 590 600
HPWLNNLAEK AKRCNRRLKS QILLKKYLMK RRWKKNFIAV SAANRFKKIS
610
SSGALMALGV
Length:610
Mass (Da):65,816
Last modified:January 23, 2007 - v2
Checksum:iE26BF76EFF88701F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03886 mRNA. Translation: AAA41625.1. Sequence problems.
PIRiA28798.
RefSeqiNP_476557.1. NM_057209.1.
UniGeneiRn.81191.

Genome annotation databases

GeneIDi117558.
KEGGirno:117558.
UCSCiRGD:620934. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03886 mRNA. Translation: AAA41625.1. Sequence problems.
PIRiA28798.
RefSeqiNP_476557.1. NM_057209.1.
UniGeneiRn.81191.

3D structure databases

ProteinModelPortaliP20689.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20689. 2 interactions.
MINTiMINT-1346460.
STRINGi10116.ENSRNOP00000011255.

PTM databases

iPTMnetiP20689.
PhosphoSiteiP20689.

Proteomic databases

PaxDbiP20689.
PRIDEiP20689.

Protocols and materials databases

DNASUi117558.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi117558.
KEGGirno:117558.
UCSCiRGD:620934. rat.

Organism-specific databases

CTDi85366.
RGDi620934. Mylk2.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233016.
HOVERGENiHBG080416.
InParanoidiP20689.
KOiK00907.
PhylomeDBiP20689.

Enzyme and pathway databases

BRENDAi2.7.11.18. 5301.

Miscellaneous databases

PROiP20689.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of the cDNA encoding rat skeletal muscle myosin light chain kinase. Sequence and tissue distribution."
    Roush C.L., Kennelly P.J., Glaccum M.B., Helfman D.M., Scott J.D., Krebs E.G.
    J. Biol. Chem. 263:10510-10516(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular characterization of rat skeletal muscle myosin light chain kinase."
    Herring B.P., Nunnally M.H., Gallagher P.J., Stull J.T.
    Am. J. Physiol. 256:C399-C404(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 296-610.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-459, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMYLK2_RAT
AccessioniPrimary (citable) accession number: P20689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.