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Protein

Nucleoporin NUP1

Gene

NUP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). As one of the FG repeat nucleoporins NUP1 is involved in interactions with and guidance of nuclear transport receptors such as SRP1-KAP95 (importin alpha and beta) through the NPC. Like the closely related NUP2 it also plays an important role in disassembling and recycling SRP1-KAP95 to the cytoplasm after nuclear import. Upon entry of the heterotrimeric SRP1-KAP95-cargo complex in the nucleus, NUP1 binds through its C-terminus to KAP95, thus accelerating the release of KAP95 and, indirectly, of the nuclear localization signal (NLS)-containing cargo from the SRP1-KAP95-cargo complex.9 Publications

GO - Molecular functioni

  • nuclear localization sequence binding Source: GO_Central
  • nucleocytoplasmic transporter activity Source: SGD
  • structural constituent of nuclear pore Source: SGD

GO - Biological processi

  • NLS-bearing protein import into nucleus Source: SGD
  • poly(A)+ mRNA export from nucleus Source: SGD
  • protein import into nucleus Source: SGD
  • ribosomal large subunit export from nucleus Source: SGD
  • transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33631-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP1
Alternative name(s):
Nuclear pore protein NUP1
Gene namesi
Name:NUP1
Ordered Locus Names:YOR098C
ORF Names:YOR3182C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR098C.
SGDiS000005624. NUP1.

Subcellular locationi

GO - Cellular componenti

  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: SGD
  • nuclear pore central transport channel Source: SGD
  • nuclear pore nuclear basket Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002049022 – 1076Nucleoporin NUP1Add BLAST1075

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei54PhosphoserineCombined sources1
Modified residuei161PhosphoserineCombined sources1
Modified residuei381PhosphothreonineCombined sources1
Modified residuei383PhosphoserineCombined sources1
Modified residuei637PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by CDC28.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP20676.
PRIDEiP20676.

PTM databases

iPTMnetiP20676.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Interacts through its FG repeats with nuclear transport receptors. Binds to the nuclear basket of the NPC through NUP60. Interacts with KAP122.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KAP95Q061426EBI-12392,EBI-9145

Protein-protein interaction databases

BioGridi34496. 77 interactors.
DIPiDIP-81N.
IntActiP20676. 47 interactors.
MINTiMINT-1759946.

Structurei

Secondary structure

11076
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1004 – 1006Combined sources3
Turni1007 – 1009Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BPTX-ray1.99B974-1012[»]
4C31X-ray3.00C/F/X/Y322-355[»]
4MBEX-ray2.61G/H/X/Y316-340[»]
ProteinModelPortaliP20676.
SMRiP20676.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20676.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati336 – 338FXF 13
Repeati384 – 386FXF 23
Repeati406 – 409FXFG 14
Repeati422 – 425FXFG 24
Repeati448 – 451FXFG 34
Repeati484 – 487FXFG 44
Repeati510 – 513FXFG 54
Repeati525 – 528FXFG 64
Repeati543 – 546FXFG 74
Repeati571 – 574FXFG 84
Repeati591 – 593FXF 33
Repeati614 – 616FXF 43
Repeati636 – 638FXF 53
Repeati657 – 659FXF 63
Repeati671 – 674FXFG 94
Repeati689 – 691FXF 73
Repeati708 – 711FXFG 104
Repeati727 – 730FXFG 114
Repeati753 – 755FXF 83
Repeati800 – 803FXFG 124
Repeati819 – 821FXF 93
Repeati866 – 868FXF 103
Repeati885 – 888FXFG 134
Repeati929 – 931FXF 113
Repeati1008 – 1009FG 12
Repeati1027 – 1028FG 22
Repeati1038 – 1039FG 32

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1040 – 1076Interaction with KAP95Add BLAST37

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi81 – 84Poly-Asn4
Compositional biasi718 – 723Poly-Thr6
Compositional biasi830 – 833Poly-Thr4
Compositional biasi881 – 1022Asn-richAdd BLAST142

Domaini

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side: FXFG repeats are especially abundant in NUPs on the nucleoplasmic side (in a highly charged environment and enriched in Ser and Thr).

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00730000111010.
InParanoidiP20676.
KOiK18721.
OMAiFEYDLSE.
OrthoDBiEOG092C4QBH.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNTSSVMS SPRVEKRSFS STLKSFFTNP NKKRPSSKKV FSSNLSYANH
60 70 80 90 100
LEESDVEDTL HVNKRKRVSG TSQHSDSLTQ NNNNAPIIIY GTENTERPPL
110 120 130 140 150
LPILPIQRLR LLREKQRVRN MRELGLIQST EFPSITSSVI LGSQSKSDEG
160 170 180 190 200
GSYLCTSSTP SPIKNGSCTR QLAGKSGEDT NVGLPILKSL KNRSNRKRFH
210 220 230 240 250
SQSKGTVWSA NFEYDLSEYD AIQKKDNKDK EGNAGGDQKT SENRNNIKSS
260 270 280 290 300
ISNGNLATGP NLTSEIEDLR ADINSNRLSN PQKNLLLKGP ASTVAKTAPI
310 320 330 340 350
QESFVPNSER SGTPTLKKNI EPKKDKESIV LPTVGFDFIK DNETPSKKTS
360 370 380 390 400
PKATSSAGAV FKSSVEMGKT DKSTKTAEAP TLSFNFSQKA NKTKAVDNTV
410 420 430 440 450
PSTTLFNFGG KSDTVTSASQ PFKFGKTSEK SENHTESDAP PKSTAPIFSF
460 470 480 490 500
GKQEENGDEG DDENEPKRKR RLPVSEDTNT KPLFDFGKTG DQKETKKGES
510 520 530 540 550
EKDASGKPSF VFGASDKQAE GTPLFTFGKK ADVTSNIDSS AQFTFGKAAT
560 570 580 590 600
AKETHTKPSE TPATIVKKPT FTFGQSTSEN KISEGSAKPT FSFSKSEEER
610 620 630 640 650
KSSPISNEAA KPSFSFPGKP VDVQAPTDDK TLKPTFSFTE PAQKDSSVVS
660 670 680 690 700
EPKKPSFTFA SSKTSQPKPL FSFGKSDAAK EPPGSNTSFS FTKPPANETD
710 720 730 740 750
KRPTPPSFTF GGSTTNNTTT TSTKPSFSFG APESMKSTAS TAAANTEKLS
760 770 780 790 800
NGFSFTKFNH NKEKSNSPTS FFDGSASSTP IPVLGKPTDA TGNTTSKSAF
810 820 830 840 850
SFGTANTNGT NASANSTSFS FNAPATGNGT TTTSNTSGTN IAGTFNVGKP
860 870 880 890 900
DQSIASGNTN GAGSAFGFSS SGTAATGAAS NQSSFNFGNN GAGGLNPFTS
910 920 930 940 950
ATSSTNANAG LFNKPPSTNA QNVNVPSAFN FTGNNSTPGG GSVFNMNGNT
960 970 980 990 1000
NANTVFAGSN NQPHQSQTPS FNTNSSFTPS TVPNINFSGL NGGITNTATN
1010 1020 1030 1040 1050
ALRPSDIFGA NAASGSNSNV TNPSSIFGGA GGVPTTSFGQ PQSAPNQMGM
1060 1070
GTNNGMSMGG GVMANRKIAR MRHSKR
Length:1,076
Mass (Da):113,581
Last modified:February 1, 1991 - v1
Checksum:i4AC23567D2FB53CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33632 Genomic DNA. Translation: AAA34822.1.
X94335 Genomic DNA. Translation: CAA64020.1.
Z75006 Genomic DNA. Translation: CAA99295.1.
BK006948 Genomic DNA. Translation: DAA10875.1.
PIRiA35622.
RefSeqiNP_014741.1. NM_001183517.1.

Genome annotation databases

EnsemblFungiiYOR098C; YOR098C; YOR098C.
GeneIDi854265.
KEGGisce:YOR098C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33632 Genomic DNA. Translation: AAA34822.1.
X94335 Genomic DNA. Translation: CAA64020.1.
Z75006 Genomic DNA. Translation: CAA99295.1.
BK006948 Genomic DNA. Translation: DAA10875.1.
PIRiA35622.
RefSeqiNP_014741.1. NM_001183517.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BPTX-ray1.99B974-1012[»]
4C31X-ray3.00C/F/X/Y322-355[»]
4MBEX-ray2.61G/H/X/Y316-340[»]
ProteinModelPortaliP20676.
SMRiP20676.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34496. 77 interactors.
DIPiDIP-81N.
IntActiP20676. 47 interactors.
MINTiMINT-1759946.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiP20676.

Proteomic databases

MaxQBiP20676.
PRIDEiP20676.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR098C; YOR098C; YOR098C.
GeneIDi854265.
KEGGisce:YOR098C.

Organism-specific databases

EuPathDBiFungiDB:YOR098C.
SGDiS000005624. NUP1.

Phylogenomic databases

GeneTreeiENSGT00730000111010.
InParanoidiP20676.
KOiK18721.
OMAiFEYDLSE.
OrthoDBiEOG092C4QBH.

Enzyme and pathway databases

BioCyciYEAST:G3O-33631-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP20676.
PROiP20676.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiNUP1_YEAST
AccessioniPrimary (citable) accession number: P20676
Secondary accession number(s): D6W2F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 468 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.