Reviewed, UniProtKB/Swiss-Prot P20655 (RIPS_MOMCO)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein momorcochin-S     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Momordica cochinchinensis (Spiny bitter cucumber)
Taxonomic identifier	3674 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Momordica

Protein attributes

Sequence length	30 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inactivates eukaryotic 60S ribosomal subunits.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Post-translational modification	Glycosylated. Ref.1
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›30	›30	Ribosome-inactivating protein momorcochin-S		PRO_0000221410
Experimental info
	Non-terminal residue	30	1

Sequences

	Sequence		Length	Mass (Da)
	P20655-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 5B93CE73B9E3E221	FASTA	30	3,323
	10 20 30 DVTFSLLGAN TKSYAAFITN FRKDVASEKK

References

[1]

"Purification and properties of a new ribosome-inactivating protein with RNA N-glycosidase activity suitable for immunotoxin preparation from the seeds of Momordica cochinchinensis." Bolognesi A., Barbieri L., Carnicelli D., Abbondanza A., Cenini P., Falasca A.I., Dinota A., Stirpe F. Biochim. Biophys. Acta 993:287-292(1989) [PubMed: 2597699] [Abstract] Cited for: PROTEIN SEQUENCE, GLYCOSYLATION. Tissue: Seed.

Cross-references

Sequence databases
PIR	S07065.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 273564.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. [Graphical view]
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIPS_MOMCO

Accession

Primary (citable) accession number: P20655

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)