Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P20651

- PP2BB_RAT

UniProt

P20651 - PP2BB_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform

Gene

Ppp3cb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 1 Fe3+ ion per subunit.By similarity
Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991IronBy similarity
Metal bindingi101 – 1011IronBy similarity
Metal bindingi127 – 1271IronBy similarity
Metal bindingi127 – 1271ZincBy similarity
Metal bindingi159 – 1591ZincBy similarity
Active sitei160 – 1601Proton donorBy similarity
Metal bindingi208 – 2081ZincBy similarity
Metal bindingi290 – 2901ZincBy similarity

GO - Molecular functioni

  1. calcium-dependent protein serine/threonine phosphatase activity Source: RGD
  2. calcium ion binding Source: UniProtKB
  3. calmodulin binding Source: RGD
  4. calmodulin-dependent protein phosphatase activity Source: UniProtKB
  5. drug binding Source: UniProtKB
  6. enzyme binding Source: UniProtKB
  7. protein heterodimerization activity Source: RGD
  8. protein phosphatase 2B binding Source: UniProtKB
  9. protein serine/threonine phosphatase activity Source: Reactome

GO - Biological processi

  1. calcium ion-dependent exocytosis Source: UniProtKB
  2. cellular response to drug Source: UniProtKB
  3. muscle cell cellular homeostasis Source: RGD
  4. positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  5. protein dephosphorylation Source: RGD
  6. protein phosphorylation Source: UniProtKB
  7. regulation of insulin secretion Source: UniProtKB
  8. response to amphetamine Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calmodulin-binding, Iron, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (EC:3.1.3.16)
Alternative name(s):
CAM-PRP catalytic subunit
Calmodulin-dependent calcineurin A subunit beta isoform
Gene namesi
Name:Ppp3cb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3383. Ppp3cb.

Subcellular locationi

GO - Cellular componenti

  1. calcineurin complex Source: UniProtKB
  2. cytosol Source: Reactome
  3. plasma membrane Source: UniProtKB
  4. protein complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 525524Serine/threonine-protein phosphatase 2B catalytic subunit beta isoformPRO_0000058827Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP20651.
PRIDEiP20651.

PTM databases

PhosphoSiteiP20651.

Expressioni

Gene expression databases

GenevestigatoriP20651.

Interactioni

Subunit structurei

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Akap6Q9WVC72EBI-7400670,EBI-7559840

Protein-protein interaction databases

BioGridi246807. 1 interaction.
DIPiDIP-66N.
IntActiP20651. 4 interactions.
MINTiMINT-4589009.
STRINGi10116.ENSRNOP00000010476.

Structurei

3D structure databases

ProteinModelPortaliP20651.
SMRiP20651. Positions 24-381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 310309CatalyticAdd
BLAST
Regioni256 – 2627Calcineurin B binding-site 1Sequence Analysis
Regioni305 – 3106Calcineurin B binding-site 2Sequence Analysis
Regioni402 – 42423Calmodulin-bindingSequence AnalysisAdd
BLAST
Regioni475 – 49723Inhibitory domainAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 2010Poly-Pro

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172699.
HOVERGENiHBG002819.
InParanoidiP20651.
KOiK04348.
PhylomeDBiP20651.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20651-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAPEPARAA PPPPPPPPPP LGADRVVKAV PFPPTHRLTS EEVFDMDGIP
60 70 80 90 100
RVDVLKNHLV KEGRVDEEIA LRIINEGAAI LRREKTMIEV EAPITVCGDI
110 120 130 140 150
HGQFFDLMKL FEVGGSPANT RYLFLGDYVD RGYFSIECVL YLWVLKILYP
160 170 180 190 200
STLFLLRGNH ECRHLTEYFT FKQECKIKYS ERVYEACMEA FDSLPLAALL
210 220 230 240 250
NQQFLCVHGG LSPEIHTLDD IRRLDRFKEP PAFGPMCDLL WSDPSEDFGN
260 270 280 290 300
EKSQEHFSHN TVRGCSYFYN YPAVCEFLQN NNLLSIIRAH EAQDAGYRMY
310 320 330 340 350
RKSQTTGFPS LITIFSAPNY LDVYNNKAAV LKYENNVMNI RQFNCSPHPY
360 370 380 390 400
WLPNFMDVFT WSLPFVGEKV TEMLVNVLSI CSDDELMTEG EDQFDVGSAA
410 420 430 440 450
ARKEIIRNKI RAIGKMARVF SVLREESESV LTLKGLTPTG MLPSGVLAGG
460 470 480 490 500
RQTLQSATVE AIEAEKAIRG SSPPHRICSF EEAKGLDRIN ERMPPRKDAV
510 520
QQDGFNSLNT AHTTENHGTG NHSAQ
Length:525
Mass (Da):59,113
Last modified:February 1, 1991 - v1
Checksum:i5E66AF3100BE3987
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31809 mRNA. Translation: AAA40848.1.
D90036 mRNA. Translation: BAA14084.1.
M58441 mRNA. Translation: AAA41915.1.
PIRiA33794.
RefSeqiNP_058738.1. NM_017042.2.
UniGeneiRn.11063.

Genome annotation databases

GeneIDi24675.
KEGGirno:24675.
UCSCiRGD:3383. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31809 mRNA. Translation: AAA40848.1 .
D90036 mRNA. Translation: BAA14084.1 .
M58441 mRNA. Translation: AAA41915.1 .
PIRi A33794.
RefSeqi NP_058738.1. NM_017042.2.
UniGenei Rn.11063.

3D structure databases

ProteinModelPortali P20651.
SMRi P20651. Positions 24-381.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246807. 1 interaction.
DIPi DIP-66N.
IntActi P20651. 4 interactions.
MINTi MINT-4589009.
STRINGi 10116.ENSRNOP00000010476.

PTM databases

PhosphoSitei P20651.

Proteomic databases

PaxDbi P20651.
PRIDEi P20651.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24675.
KEGGi rno:24675.
UCSCi RGD:3383. rat.

Organism-specific databases

CTDi 5532.
RGDi 3383. Ppp3cb.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000172699.
HOVERGENi HBG002819.
InParanoidi P20651.
KOi K04348.
PhylomeDBi P20651.

Miscellaneous databases

NextBioi 604059.

Gene expression databases

Genevestigatori P20651.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evidence for a second isoform of the catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin A)."
    Kuno T., Takeda T., Hirai M., Ito A., Mukai H., Tanaka C.
    Biochem. Biophys. Res. Commun. 165:1352-1358(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Multiplicity of protein serine-threonine phosphatases in PC12 pheochromocytoma and FTO-2B hepatoma cells."
    Wadzinski B.E., Heasley L.E., Johnson G.L.
    J. Biol. Chem. 265:21504-21508(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 245-315.

Entry informationi

Entry nameiPP2BB_RAT
AccessioniPrimary (citable) accession number: P20651
Secondary accession number(s): Q6LDJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3