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P20651

- PP2BB_RAT

UniProt

P20651 - PP2BB_RAT

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Protein
Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
Gene
Ppp3cb
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 1 Fe3+ ion per subunit By similarity.
Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Iron By similarity
Metal bindingi101 – 1011Iron By similarity
Metal bindingi127 – 1271Iron By similarity
Metal bindingi127 – 1271Zinc By similarity
Metal bindingi159 – 1591Zinc By similarity
Active sitei160 – 1601Proton donor By similarity
Metal bindingi208 – 2081Zinc By similarity
Metal bindingi290 – 2901Zinc By similarity

GO - Molecular functioni

  1. calcium ion binding Source: UniProtKB
  2. calcium-dependent protein serine/threonine phosphatase activity Source: RGD
  3. calmodulin binding Source: RGD
  4. calmodulin-dependent protein phosphatase activity Source: UniProtKB
  5. drug binding Source: UniProtKB
  6. enzyme binding Source: UniProtKB
  7. protein binding Source: IntAct
  8. protein heterodimerization activity Source: RGD
  9. protein phosphatase 2B binding Source: UniProtKB
  10. protein serine/threonine phosphatase activity Source: Reactome

GO - Biological processi

  1. calcium ion-dependent exocytosis Source: UniProtKB
  2. cellular response to drug Source: UniProtKB
  3. muscle cell cellular homeostasis Source: RGD
  4. positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  5. protein dephosphorylation Source: RGD
  6. protein phosphorylation Source: UniProtKB
  7. regulation of insulin secretion Source: UniProtKB
  8. response to amphetamine Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calmodulin-binding, Iron, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (EC:3.1.3.16)
Alternative name(s):
CAM-PRP catalytic subunit
Calmodulin-dependent calcineurin A subunit beta isoform
Gene namesi
Name:Ppp3cb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3383. Ppp3cb.

Subcellular locationi

GO - Cellular componenti

  1. calcineurin complex Source: UniProtKB
  2. cytosol Source: Reactome
  3. plasma membrane Source: UniProtKB
  4. protein complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 525524Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
PRO_0000058827Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP20651.
PRIDEiP20651.

PTM databases

PhosphoSiteiP20651.

Expressioni

Gene expression databases

GenevestigatoriP20651.

Interactioni

Subunit structurei

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Akap6Q9WVC72EBI-7400670,EBI-7559840

Protein-protein interaction databases

BioGridi246807. 1 interaction.
DIPiDIP-66N.
IntActiP20651. 4 interactions.
MINTiMINT-4589009.
STRINGi10116.ENSRNOP00000010476.

Structurei

3D structure databases

ProteinModelPortaliP20651.
SMRiP20651. Positions 24-381.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 310309Catalytic
Add
BLAST
Regioni256 – 2627Calcineurin B binding-site 1 Reviewed prediction
Regioni305 – 3106Calcineurin B binding-site 2 Reviewed prediction
Regioni402 – 42423Calmodulin-binding Reviewed prediction
Add
BLAST
Regioni475 – 49723Inhibitory domain
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 2010Poly-Pro

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172699.
HOVERGENiHBG002819.
InParanoidiP20651.
KOiK04348.
PhylomeDBiP20651.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20651-1 [UniParc]FASTAAdd to Basket

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MAAPEPARAA PPPPPPPPPP LGADRVVKAV PFPPTHRLTS EEVFDMDGIP    50
RVDVLKNHLV KEGRVDEEIA LRIINEGAAI LRREKTMIEV EAPITVCGDI 100
HGQFFDLMKL FEVGGSPANT RYLFLGDYVD RGYFSIECVL YLWVLKILYP 150
STLFLLRGNH ECRHLTEYFT FKQECKIKYS ERVYEACMEA FDSLPLAALL 200
NQQFLCVHGG LSPEIHTLDD IRRLDRFKEP PAFGPMCDLL WSDPSEDFGN 250
EKSQEHFSHN TVRGCSYFYN YPAVCEFLQN NNLLSIIRAH EAQDAGYRMY 300
RKSQTTGFPS LITIFSAPNY LDVYNNKAAV LKYENNVMNI RQFNCSPHPY 350
WLPNFMDVFT WSLPFVGEKV TEMLVNVLSI CSDDELMTEG EDQFDVGSAA 400
ARKEIIRNKI RAIGKMARVF SVLREESESV LTLKGLTPTG MLPSGVLAGG 450
RQTLQSATVE AIEAEKAIRG SSPPHRICSF EEAKGLDRIN ERMPPRKDAV 500
QQDGFNSLNT AHTTENHGTG NHSAQ 525
Length:525
Mass (Da):59,113
Last modified:February 1, 1991 - v1
Checksum:i5E66AF3100BE3987
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31809 mRNA. Translation: AAA40848.1.
D90036 mRNA. Translation: BAA14084.1.
M58441 mRNA. Translation: AAA41915.1.
PIRiA33794.
RefSeqiNP_058738.1. NM_017042.2.
UniGeneiRn.11063.

Genome annotation databases

GeneIDi24675.
KEGGirno:24675.
UCSCiRGD:3383. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31809 mRNA. Translation: AAA40848.1 .
D90036 mRNA. Translation: BAA14084.1 .
M58441 mRNA. Translation: AAA41915.1 .
PIRi A33794.
RefSeqi NP_058738.1. NM_017042.2.
UniGenei Rn.11063.

3D structure databases

ProteinModelPortali P20651.
SMRi P20651. Positions 24-381.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246807. 1 interaction.
DIPi DIP-66N.
IntActi P20651. 4 interactions.
MINTi MINT-4589009.
STRINGi 10116.ENSRNOP00000010476.

PTM databases

PhosphoSitei P20651.

Proteomic databases

PaxDbi P20651.
PRIDEi P20651.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24675.
KEGGi rno:24675.
UCSCi RGD:3383. rat.

Organism-specific databases

CTDi 5532.
RGDi 3383. Ppp3cb.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000172699.
HOVERGENi HBG002819.
InParanoidi P20651.
KOi K04348.
PhylomeDBi P20651.

Miscellaneous databases

NextBioi 604059.

Gene expression databases

Genevestigatori P20651.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evidence for a second isoform of the catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin A)."
    Kuno T., Takeda T., Hirai M., Ito A., Mukai H., Tanaka C.
    Biochem. Biophys. Res. Commun. 165:1352-1358(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Multiplicity of protein serine-threonine phosphatases in PC12 pheochromocytoma and FTO-2B hepatoma cells."
    Wadzinski B.E., Heasley L.E., Johnson G.L.
    J. Biol. Chem. 265:21504-21508(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 245-315.

Entry informationi

Entry nameiPP2BB_RAT
AccessioniPrimary (citable) accession number: P20651
Secondary accession number(s): Q6LDJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 3, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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