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P20650

- PPM1A_RAT

UniProt

P20650 - PPM1A_RAT

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Protein
Protein phosphatase 1A
Gene
Ppm1a, Pp2c1, Pppm1a
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling By similarity. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB By similarity.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Manganese 1 By similarity
Metal bindingi60 – 601Manganese 2 By similarity
Metal bindingi61 – 611Manganese 1; via carbonyl oxygen By similarity
Metal bindingi239 – 2391Manganese 2 By similarity
Metal bindingi282 – 2821Manganese 2 By similarity

GO - Molecular functioni

  1. R-SMAD binding Source: UniProtKB
  2. calmodulin-dependent protein phosphatase activity Source: Ensembl
  3. magnesium ion binding Source: InterPro
  4. manganese ion binding Source: InterPro
  5. protein C-terminus binding Source: UniProtKB
  6. protein binding Source: IntAct
  7. signal transducer activity Source: Ensembl
Complete GO annotation...

GO - Biological processi

  1. N-terminal protein myristoylation Source: UniProtKB
  2. Wnt signaling pathway Source: Ensembl
  3. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  4. negative regulation of NF-kappaB import into nucleus Source: UniProtKB
  5. negative regulation of SMAD protein complex assembly Source: Ensembl
  6. negative regulation of transforming growth factor beta receptor signaling pathway Source: Ensembl
  7. peptidyl-threonine dephosphorylation Source: Ensembl
  8. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
  9. positive regulation of Wnt signaling pathway Source: Ensembl
  10. positive regulation of transcription, DNA-templated Source: Ensembl
  11. protein dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_194639. Downregulation of SMAD2/3:SMAD4 transcriptional activity.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1A (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2C isoform alpha
Short name:
PP2C-alpha
Protein phosphatase IA
Gene namesi
Name:Ppm1a
Synonyms:Pp2c1, Pppm1a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 6

Organism-specific databases

RGDi3373. Ppm1a.

Subcellular locationi

Nucleus By similarity. Cytoplasmcytosol By similarity. Membrane By similarity
Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization By similarity.

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. membrane Source: UniProtKB
  3. neuron projection Source: UniProtKB
  4. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 382381Protein phosphatase 1A
PRO_0000057744Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine By similarity
Modified residuei375 – 3751Phosphoserine By similarity

Post-translational modificationi

N-myristoylation is essential for the recognition of its substrates for dephosphorylation By similarity.

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiP20650.
PRIDEiP20650.

PTM databases

PhosphoSiteiP20650.

Expressioni

Gene expression databases

ArrayExpressiP20650.
GenevestigatoriP20650.

Interactioni

Subunit structurei

Monomer By similarity. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling By similarity. Interacts with the phosphorylated form of IKBKB/IKKB By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
CACNA1BQ009753EBI-7491743,EBI-1055161From a different organism.

Protein-protein interaction databases

BioGridi246798. 1 interaction.
IntActiP20650. 4 interactions.
MINTiMINT-4588994.
STRINGi10116.ENSRNOP00000008238.

Structurei

3D structure databases

ProteinModelPortaliP20650.
SMRiP20650. Positions 2-368.

Family & Domainsi

Sequence similaritiesi

Belongs to the PP2C family.

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000115384.
HOGENOMiHOG000233895.
HOVERGENiHBG053647.
InParanoidiP20650.
KOiK04457.
OMAiEVYAIER.
OrthoDBiEOG7WMCJH.
PhylomeDBiP20650.
TreeFamiTF313590.

Family and domain databases

Gene3Di1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20650-1 [UniParc]FASTAAdd to Basket

« Hide

MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL    50
ETWSFFAVYD GHAGSQVAKY CCEHLLDHIT NNQDFKGSAG APSVENVKNG 100
IRTGFLEIDE HMRVMSEKKH GADRSGSTAV GVLISPQHTY FINCGDSRGL 150
LCRNRKVHFF TQDHKPSNPL EKERIQNAGG SVMIQRVNGS LAVSRALGDF 200
DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG IWDVMGNEEL 250
CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPNAPKVSAE 300
AVKKEAELDK YLENRVEEII KKQGEGVPDL VHVMRTLASE NIPSLPPGGE 350
LASKRNVIEA VYNRLNPYKN DDTDSASTDD MW 382
Length:382
Mass (Da):42,417
Last modified:February 1, 1991 - v1
Checksum:iC1C386E935374F89
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04503 mRNA. Translation: AAA41917.1.
PIRiA32399.
RefSeqiNP_058734.1. NM_017038.1.
XP_006240257.1. XM_006240195.1.
UniGeneiRn.37403.

Genome annotation databases

EnsembliENSRNOT00000008238; ENSRNOP00000008238; ENSRNOG00000005916.
GeneIDi24666.
KEGGirno:24666.
UCSCiRGD:3373. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04503 mRNA. Translation: AAA41917.1 .
PIRi A32399.
RefSeqi NP_058734.1. NM_017038.1.
XP_006240257.1. XM_006240195.1.
UniGenei Rn.37403.

3D structure databases

ProteinModelPortali P20650.
SMRi P20650. Positions 2-368.
ModBasei Search...

Protein-protein interaction databases

BioGridi 246798. 1 interaction.
IntActi P20650. 4 interactions.
MINTi MINT-4588994.
STRINGi 10116.ENSRNOP00000008238.

PTM databases

PhosphoSitei P20650.

Proteomic databases

PaxDbi P20650.
PRIDEi P20650.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000008238 ; ENSRNOP00000008238 ; ENSRNOG00000005916 .
GeneIDi 24666.
KEGGi rno:24666.
UCSCi RGD:3373. rat.

Organism-specific databases

CTDi 5494.
RGDi 3373. Ppm1a.

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00740000115384.
HOGENOMi HOG000233895.
HOVERGENi HBG053647.
InParanoidi P20650.
KOi K04457.
OMAi EVYAIER.
OrthoDBi EOG7WMCJH.
PhylomeDBi P20650.
TreeFami TF313590.

Enzyme and pathway databases

Reactomei REACT_194639. Downregulation of SMAD2/3:SMAD4 transcriptional activity.

Miscellaneous databases

NextBioi 604026.
PROi P20650.

Gene expression databases

ArrayExpressi P20650.
Genevestigatori P20650.

Family and domain databases

Gene3Di 1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Kidney.

Entry informationi

Entry nameiPPM1A_RAT
AccessioniPrimary (citable) accession number: P20650
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 3, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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