P20650 (PPM1A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1A
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 2C isoform alpha
Short name=PP2C-alpha
Protein phosphatase IA

Gene names

Name:	Ppm1a
Synonyms:	Pp2c1, Pppm1a

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit.
Subunit structure	Monomer By similarity. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of SMAD protein complex assembly Inferred from electronic annotation. Source: Compara negative regulation of transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara peptidyl-threonine dephosphorylation Inferred from electronic annotation. Source: Compara positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from electronic annotation. Source: Compara positive regulation of Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara
Cellular_component	neuron projection Inferred from direct assay PubMed 15728831. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	R-SMAD binding Inferred from sequence or structural similarity. Source: UniProtKB calmodulin-dependent protein phosphatase activity Inferred from electronic annotation. Source: Compara magnesium ion binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: InterPro signal transducer activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Protein phosphatase 1A		PRO_0000057744
Sites
	Metal binding	60	1	Manganese 1 By similarity
	Metal binding	60	1	Manganese 2 By similarity
	Metal binding	61	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	239	1	Manganese 2 By similarity
	Metal binding	282	1	Manganese 2 By similarity
Amino acid modifications
	Modified residue	375	1	Phosphoserine By similarity
	Modified residue	377	1	Phosphoserine By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20650 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: C1C386E935374F89	FASTA	382	42,417
	10 20 30 40 50 60 MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL ETWSFFAVYD 70 80 90 100 110 120 GHAGSQVAKY CCEHLLDHIT NNQDFKGSAG APSVENVKNG IRTGFLEIDE HMRVMSEKKH 130 140 150 160 170 180 GADRSGSTAV GVLISPQHTY FINCGDSRGL LCRNRKVHFF TQDHKPSNPL EKERIQNAGG 190 200 210 220 230 240 SVMIQRVNGS LAVSRALGDF DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG 250 260 270 280 290 300 IWDVMGNEEL CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPNAPKVSAE 310 320 330 340 350 360 AVKKEAELDK YLENRVEEII KKQGEGVPDL VHVMRTLASE NIPSLPPGGE LASKRNVIEA 370 380 VYNRLNPYKN DDTDSASTDD MW

References

[1]	"Molecular cloning of rat type 2C (IA) protein phosphatase mRNA." Tamura S., Lynch K.R., Larner J., Fox J., Yasui A., Kikuchi K., Suzuki Y., Tsuiki S. Proc. Natl. Acad. Sci. U.S.A. 86:1796-1800(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04503 mRNA. Translation: AAA41917.1.
IPI	IPI00201403.
PIR	A32399.
RefSeq	NP_058734.1. NM_017038.1.
UniGene	Rn.37403.
3D structure databases
ProteinModelPortal	P20650.
SMR	P20650. Positions 2-368.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4588994.
STRING	10116.ENSRNOP00000008238.
PTM databases
PhosphoSite	P20650.
Proteomic databases
PaxDb	P20650.
PRIDE	P20650.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000008238; ENSRNOP00000008238; ENSRNOG00000005916.
GeneID	24666.
KEGG	rno:24666.
UCSC	RGD:3373. rat.
Organism-specific databases
CTD	5494.
RGD	3373. Ppm1a.
Phylogenomic databases
eggNOG	COG0631.
GeneTree	ENSGT00650000093052.
HOGENOM	HOG000233895.
HOVERGEN	HBG053647.
InParanoid	P20650.
KO	K04457.
OMA	EVYAIER.
Gene expression databases
ArrayExpress	P20650.
Genevestigator	P20650.
GermOnline	ENSRNOG00000005916. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.10.430. 1 hit. 3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR012911. PP2C_C. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. PF07830. PP2C_C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit. SSF81601. PP2C_C. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	604026.

Entry information

Entry name

PPM1A_RAT

Accession

Primary (citable) accession number: P20650

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program