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P20650

- PPM1A_RAT

UniProt

P20650 - PPM1A_RAT

Protein

Protein phosphatase 1A

Gene

Ppm1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling By similarity. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB By similarity.By similarity

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi60 – 601Manganese 1By similarity
    Metal bindingi60 – 601Manganese 2By similarity
    Metal bindingi61 – 611Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi239 – 2391Manganese 2By similarity
    Metal bindingi282 – 2821Manganese 2By similarity

    GO - Molecular functioni

    1. calmodulin-dependent protein phosphatase activity Source: Ensembl
    2. magnesium ion binding Source: InterPro
    3. manganese ion binding Source: InterPro
    4. protein binding Source: IntAct
    5. protein C-terminus binding Source: UniProtKB
    6. R-SMAD binding Source: UniProtKB
    7. signal transducer activity Source: Ensembl

    GO - Biological processi

    1. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
    2. negative regulation of NF-kappaB import into nucleus Source: UniProtKB
    3. negative regulation of SMAD protein complex assembly Source: Ensembl
    4. negative regulation of transforming growth factor beta receptor signaling pathway Source: Ensembl
    5. N-terminal protein myristoylation Source: UniProtKB
    6. peptidyl-threonine dephosphorylation Source: Ensembl
    7. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
    8. positive regulation of transcription, DNA-templated Source: Ensembl
    9. positive regulation of Wnt signaling pathway Source: Ensembl
    10. protein dephosphorylation Source: UniProtKB
    11. Wnt signaling pathway Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_194639. Downregulation of SMAD2/3:SMAD4 transcriptional activity.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 1A (EC:3.1.3.16)
    Alternative name(s):
    Protein phosphatase 2C isoform alpha
    Short name:
    PP2C-alpha
    Protein phosphatase IA
    Gene namesi
    Name:Ppm1a
    Synonyms:Pp2c1, Pppm1a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 6

    Organism-specific databases

    RGDi3373. Ppm1a.

    Subcellular locationi

    Nucleus By similarity. Cytoplasmcytosol By similarity. Membrane By similarity
    Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization.By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. membrane Source: UniProtKB
    3. neuron projection Source: UniProtKB
    4. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 382381Protein phosphatase 1APRO_0000057744Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Modified residuei375 – 3751PhosphoserineBy similarity

    Post-translational modificationi

    N-myristoylation is essential for the recognition of its substrates for dephosphorylation.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Phosphoprotein

    Proteomic databases

    PaxDbiP20650.
    PRIDEiP20650.

    PTM databases

    PhosphoSiteiP20650.

    Expressioni

    Gene expression databases

    ArrayExpressiP20650.
    GenevestigatoriP20650.

    Interactioni

    Subunit structurei

    Monomer By similarity. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling By similarity. Interacts with the phosphorylated form of IKBKB/IKKB By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CACNA1BQ009753EBI-7491743,EBI-1055161From a different organism.

    Protein-protein interaction databases

    BioGridi246798. 1 interaction.
    IntActiP20650. 4 interactions.
    MINTiMINT-4588994.
    STRINGi10116.ENSRNOP00000008238.

    Structurei

    3D structure databases

    ProteinModelPortaliP20650.
    SMRiP20650. Positions 2-368.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PP2C family.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    GeneTreeiENSGT00740000115384.
    HOGENOMiHOG000233895.
    HOVERGENiHBG053647.
    InParanoidiP20650.
    KOiK04457.
    OMAiEVYAIER.
    OrthoDBiEOG7WMCJH.
    PhylomeDBiP20650.
    TreeFamiTF313590.

    Family and domain databases

    Gene3Di1.10.10.430. 1 hit.
    3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR012911. PP2C_C.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    PF07830. PP2C_C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81601. SSF81601. 1 hit.
    SSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P20650-1 [UniParc]FASTAAdd to Basket

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    MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL    50
    ETWSFFAVYD GHAGSQVAKY CCEHLLDHIT NNQDFKGSAG APSVENVKNG 100
    IRTGFLEIDE HMRVMSEKKH GADRSGSTAV GVLISPQHTY FINCGDSRGL 150
    LCRNRKVHFF TQDHKPSNPL EKERIQNAGG SVMIQRVNGS LAVSRALGDF 200
    DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG IWDVMGNEEL 250
    CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPNAPKVSAE 300
    AVKKEAELDK YLENRVEEII KKQGEGVPDL VHVMRTLASE NIPSLPPGGE 350
    LASKRNVIEA VYNRLNPYKN DDTDSASTDD MW 382
    Length:382
    Mass (Da):42,417
    Last modified:February 1, 1991 - v1
    Checksum:iC1C386E935374F89
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04503 mRNA. Translation: AAA41917.1.
    PIRiA32399.
    RefSeqiNP_058734.1. NM_017038.1.
    XP_006240257.1. XM_006240195.1.
    UniGeneiRn.37403.

    Genome annotation databases

    EnsembliENSRNOT00000008238; ENSRNOP00000008238; ENSRNOG00000005916.
    GeneIDi24666.
    KEGGirno:24666.
    UCSCiRGD:3373. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04503 mRNA. Translation: AAA41917.1 .
    PIRi A32399.
    RefSeqi NP_058734.1. NM_017038.1.
    XP_006240257.1. XM_006240195.1.
    UniGenei Rn.37403.

    3D structure databases

    ProteinModelPortali P20650.
    SMRi P20650. Positions 2-368.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246798. 1 interaction.
    IntActi P20650. 4 interactions.
    MINTi MINT-4588994.
    STRINGi 10116.ENSRNOP00000008238.

    PTM databases

    PhosphoSitei P20650.

    Proteomic databases

    PaxDbi P20650.
    PRIDEi P20650.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000008238 ; ENSRNOP00000008238 ; ENSRNOG00000005916 .
    GeneIDi 24666.
    KEGGi rno:24666.
    UCSCi RGD:3373. rat.

    Organism-specific databases

    CTDi 5494.
    RGDi 3373. Ppm1a.

    Phylogenomic databases

    eggNOGi COG0631.
    GeneTreei ENSGT00740000115384.
    HOGENOMi HOG000233895.
    HOVERGENi HBG053647.
    InParanoidi P20650.
    KOi K04457.
    OMAi EVYAIER.
    OrthoDBi EOG7WMCJH.
    PhylomeDBi P20650.
    TreeFami TF313590.

    Enzyme and pathway databases

    Reactomei REACT_194639. Downregulation of SMAD2/3:SMAD4 transcriptional activity.

    Miscellaneous databases

    NextBioi 604026.
    PROi P20650.

    Gene expression databases

    ArrayExpressi P20650.
    Genevestigatori P20650.

    Family and domain databases

    Gene3Di 1.10.10.430. 1 hit.
    3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR012911. PP2C_C.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    PF07830. PP2C_C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81601. SSF81601. 1 hit.
    SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Kidney.

    Entry informationi

    Entry nameiPPM1A_RAT
    AccessioniPrimary (citable) accession number: P20650
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3