Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATPase 1, plasma membrane-type

Gene

AHA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses. Forms a functional cation-translocating unit with CNGC17 that is activated by PSKR1/BAK1 and possibly other BAK1/RLK complexes (PubMed:26071421).1 Publication

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3294-aspartylphosphate intermediateBy similarity1
Metal bindingi588MagnesiumBy similarity1
Metal bindingi592MagnesiumBy similarity1

GO - Molecular functioni

  • ATPase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • hydrogen-exporting ATPase activity, phosphorylative mechanism Source: UniProtKB
  • magnesium ion binding Source: UniProtKB

GO - Biological processi

  • ATP biosynthetic process Source: InterPro
  • ATP hydrolysis coupled proton transport Source: UniProtKB
  • proton transport Source: TAIR
  • regulation of intracellular pH Source: GO_Central
  • regulation of stomatal movement Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to water deprivation Source: TAIR
  • stomatal opening Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G18960-MONOMER.

Protein family/group databases

TCDBi3.A.3.3.7. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase 1, plasma membrane-type1 Publication (EC:3.6.3.6Curated)
Alternative name(s):
Proton pump 1
Gene namesi
Name:AHA11 Publication
Ordered Locus Names:At2g18960Imported
ORF Names:F19F24.16Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G18960.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 61CytoplasmicSequence analysisAdd BLAST60
Transmembranei62 – 81Helical; Name=1Sequence analysisAdd BLAST20
Topological domaini82 – 93ExtracellularSequence analysisAdd BLAST12
Transmembranei94 – 114Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini115 – 243CytoplasmicSequence analysisAdd BLAST129
Transmembranei244 – 264Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini265 – 273ExtracellularSequence analysis9
Transmembranei274 – 291Helical; Name=4Sequence analysisAdd BLAST18
Topological domaini292 – 643CytoplasmicSequence analysisAdd BLAST352
Transmembranei644 – 665Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini666 – 670ExtracellularSequence analysis5
Transmembranei671 – 693Helical; Name=6Sequence analysisAdd BLAST23
Topological domaini694 – 709CytoplasmicSequence analysisAdd BLAST16
Transmembranei710 – 730Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini731 – 751ExtracellularSequence analysisAdd BLAST21
Transmembranei752 – 772Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini773 – 784CytoplasmicSequence analysisAdd BLAST12
Transmembranei785 – 805Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini806 – 813ExtracellularSequence analysis8
Transmembranei814 – 834Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini835 – 949CytoplasmicSequence analysisAdd BLAST115

GO - Cellular componenti

  • Golgi apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: GO_Central
  • membrane Source: TAIR
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, due to the redudancy with AHA2. Aha1 and aha2 double mutants are embryo lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000462742 – 949ATPase 1, plasma membrane-typeAdd BLAST948

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei881PhosphothreonineCombined sources1
Modified residuei899PhosphoserineBy similarity1
Modified residuei931PhosphoserineBy similarity1
Modified residuei948PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP20649.
PRIDEiP20649.

PTM databases

iPTMnetiP20649.
SwissPalmiP20649.

Expressioni

Developmental stagei

Expressed on the surface of developing seeds and from 8- to 16-cell stages to the heart stage of embryo development.1 Publication

Gene expression databases

GenevisibleiP20649. AT.

Interactioni

Subunit structurei

Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-948. Binding to 14-3-3 proteins activates the H+-ATPase (By similarity). Interacts with PPI1; this interaction promotes ATPase activity. Interacts with PSY1R (PubMed:25267325). Part of a functional complex containing PSKR1, BAK1, CNGC17, and AHA (PubMed:26071421). Interacts with CNGC17 and PSKR1 (PubMed:26071421).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PPI1O231447EBI-2354448,EBI-2354477

Protein-protein interaction databases

BioGridi1770. 10 interactors.
IntActiP20649. 4 interactors.
MINTiMINT-6822995.
STRINGi3702.AT2G18960.1.

Structurei

3D structure databases

ProteinModelPortaliP20649.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni947 – 949Interaction with 14-3-3 proteinsBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiP20649.
KOiK01535.
OMAiEDVNIFP.
OrthoDBiEOG093602AD.
PhylomeDBiP20649.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLEDIKNE TVDLEKIPIE EVFQQLKCTR EGLTTQEGED RIVIFGPNKL
60 70 80 90 100
EEKKESKILK FLGFMWNPLS WVMEAAALMA IALANGDNRP PDWQDFVGII
110 120 130 140 150
CLLVINSTIS FIEENNAGNA AAALMAGLAP KTKVLRDGKW SEQEAAILVP
160 170 180 190 200
GDIVSIKLGD IIPADARLLE GDPLKVDQSA LTGESLPVTK HPGQEVFSGS
210 220 230 240 250
TCKQGEIEAV VIATGVHTFF GKAAHLVDST NQVGHFQKVL TSIGNFCICS
260 270 280 290 300
IAIGIAIEIV VMYPIQHRKY RDGIDNLLVL LIGGIPIAMP TVLSVTMAIG
310 320 330 340 350
SHRLSQQGAI TKRMTAIEEM AGMDVLCSDK TGTLTLNKLS VDKNLVEVFC
360 370 380 390 400
KGVEKDQVLL FAAMASRVEN QDAIDAAMVG MLADPKEARA GIREVHFLPF
410 420 430 440 450
NPVDKRTALT YIDSDGNWHR VSKGAPEQIL DLANARPDLR KKVLSCIDKY
460 470 480 490 500
AERGLRSLAV ARQVVPEKTK ESPGGPWEFV GLLPLFDPPR HDSAETIRRA
510 520 530 540 550
LNLGVNVKMI TGDQLAIGKE TGRRLGMGTN MYPSAALLGT DKDSNIASIP
560 570 580 590 600
VEELIEKADG FAGVFPEHKY EIVKKLQERK HIVGMTGDGV NDAPALKKAD
610 620 630 640 650
IGIAVADATD AARGASDIVL TEPGLSVIIS AVLTSRAIFQ RMKNYTIYAV
660 670 680 690 700
SITIRIVFGF MLIALIWEFD FSAFMVLIIA ILNDGTIMTI SKDRVKPSPT
710 720 730 740 750
PDSWKLKEIF ATGIVLGGYQ AIMSVIFFWA AHKTDFFSDK FGVRSIRDNN
760 770 780 790 800
DELMGAVYLQ VSIISQALIF VTRSRSWSFV ERPGALLMIA FVIAQLVATL
810 820 830 840 850
IAVYADWTFA KVKGIGWGWA GVIWIYSIVT YFPQDILKFA IRYILSGKAW
860 870 880 890 900
ASLFDNRTAF TTKKDYGIGE REAQWAQAQR TLHGLQPKED VNIFPEKGSY
910 920 930 940
RELSEIAEQA KRRAEIARLR ELHTLKGHVE SVAKLKGLDI DTAGHHYTV
Length:949
Mass (Da):104,224
Last modified:January 23, 2007 - v3
Checksum:iA613FF6070ECC9F3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti771V → L in AAA32813 (PubMed:2521951).Curated1
Sequence conflicti778S → Y in AAA32813 (PubMed:2521951).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24107 mRNA. Translation: AAA32813.1.
AC003673 Genomic DNA. Translation: AAC09030.1.
CP002685 Genomic DNA. Translation: AEC06832.1.
BT008692 mRNA. Translation: AAP40498.1.
PIRiT01624. PXMUP1.
RefSeqiNP_179486.1. NM_127453.4.
UniGeneiAt.24695.

Genome annotation databases

EnsemblPlantsiAT2G18960.1; AT2G18960.1; AT2G18960.
GeneIDi816413.
GrameneiAT2G18960.1; AT2G18960.1; AT2G18960.
KEGGiath:AT2G18960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24107 mRNA. Translation: AAA32813.1.
AC003673 Genomic DNA. Translation: AAC09030.1.
CP002685 Genomic DNA. Translation: AEC06832.1.
BT008692 mRNA. Translation: AAP40498.1.
PIRiT01624. PXMUP1.
RefSeqiNP_179486.1. NM_127453.4.
UniGeneiAt.24695.

3D structure databases

ProteinModelPortaliP20649.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1770. 10 interactors.
IntActiP20649. 4 interactors.
MINTiMINT-6822995.
STRINGi3702.AT2G18960.1.

Protein family/group databases

TCDBi3.A.3.3.7. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiP20649.
SwissPalmiP20649.

Proteomic databases

PaxDbiP20649.
PRIDEiP20649.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G18960.1; AT2G18960.1; AT2G18960.
GeneIDi816413.
GrameneiAT2G18960.1; AT2G18960.1; AT2G18960.
KEGGiath:AT2G18960.

Organism-specific databases

TAIRiAT2G18960.

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiP20649.
KOiK01535.
OMAiEDVNIFP.
OrthoDBiEOG093602AD.
PhylomeDBiP20649.

Enzyme and pathway databases

BioCyciARA:AT2G18960-MONOMER.

Miscellaneous databases

PROiP20649.

Gene expression databases

GenevisibleiP20649. AT.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMA1_ARATH
AccessioniPrimary (citable) accession number: P20649
Secondary accession number(s): O64626
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 163 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.