Reviewed, UniProtKB/Swiss-Prot P20648 (ATP4A_HUMAN)

Names and origin

Protein names

Recommended name:
    Potassium-transporting ATPase alpha chain 1
    EC=3.6.3.10
Alternative name(s):
    Proton pump
    Gastric H(+)/K(+) ATPase subunit alpha

Gene names

Name:

ATP4A

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1035 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for acid production in the stomach.
Catalytic activity	ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).
Subunit structure	Composed of two subunits: alpha (catalytic) and beta.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Found in gastric mucosa.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro potassium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrogen:potassium-exchanging ATPase activity Ref.1 Traceable author statement. Source: ProtInc magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 1035	1034	Potassium-transporting ATPase alpha chain 1		PRO_0000046253
Regions
	Topological domain	2 – 98	97	Cytoplasmic Potential
	Transmembrane	99 – 119	21	Potential
	Topological domain	120 – 142	23	Lumenal Potential
	Transmembrane	143 – 163	21	Potential
	Topological domain	164 – 299	136	Cytoplasmic Potential
	Transmembrane	300 – 319	20	Potential
	Topological domain	320 – 331	12	Lumenal Potential
	Transmembrane	332 – 349	18	Potential
	Topological domain	350 – 783	434	Cytoplasmic Potential
	Transmembrane	784 – 803	20	Potential
	Topological domain	804 – 813	10	Lumenal Potential
	Transmembrane	814 – 834	21	Potential
	Topological domain	835 – 854	20	Cytoplasmic Potential
	Transmembrane	855 – 877	23	Potential
	Topological domain	878 – 929	52	Lumenal Potential
	Transmembrane	930 – 949	20	Potential
	Topological domain	950 – 963	14	Cytoplasmic Potential
	Transmembrane	964 – 982	19	Potential
	Topological domain	983 – 997	15	Lumenal Potential
	Transmembrane	998 – 1018	21	Potential
	Topological domain	1019 – 1035	17	Cytoplasmic Potential
Sites
	Active site	387	1	4-aspartylphosphate intermediate By similarity
	Metal binding	728	1	Magnesium By similarity
	Metal binding	732	1	Magnesium By similarity
Amino acid modifications
	Modified residue	954	1	Phosphoserine; by PKA By similarity
Natural variations
	Natural variant	265	1	V → A: dbSNP rs2733743. Ref.1		VAR_019428

Sequences

	Sequence		Length	Mass (Da)
	P20648-1 [UniParc]. Last modified May 29, 2007. Version 5. Checksum: E320595E7D9E0E28	FASTA	1,035	114,119
	10 20 30 40 50 60 MGKAENYELY SVELGPGPGG DMAAKMSKKK KAGGGGGKRK EKLENMKKEM EINDHQLSVA 70 80 90 100 110 120 ELEQKYQTSA TKGLSASLAA ELLLRDGPNA LRPPRGTPEY VKFARQLAGG LQCLMWVAAA 130 140 150 160 170 180 ICLIAFAIQA SEGDLTTDDN LYLAIALIAV VVVTGCFGYY QEFKSTNIIA SFKNLVPQQA 190 200 210 220 230 240 TVIRDGDKFQ INADQLVVGD LVEMKGGDRV PADIRILAAQ GCKVDNSSLT GESEPQTRSP 250 260 270 280 290 300 ECTHESPLET RNIAFFSTMC LEGTVQGLVV NTGDRTIIGR IASLASGVEN EKTPIAIEIE 310 320 330 340 350 360 HFVDIIAGLA ILFGATFFIV AMCIGYTFLR AMVFFMAIVV AYVPEGLLAT VTVCLSLTAK 370 380 390 400 410 420 RLASKNCVVK NLEAVETLGS TSVICSDKTG TLTQNRMTVS HLWFDNHIHT ADTTEDQSGQ 430 440 450 460 470 480 TFDQSSETWR ALCRVLTLCN RAAFKSGQDA VPVPKRIVIG DASETALLKF SELTLGNAMG 490 500 510 520 530 540 YRDRFPKVCE IPFNSTNKFQ LSIHTLEDPR DPRHLLVMKG APERVLERCS SILIKGQELP 550 560 570 580 590 600 LDEQWREAFQ TAYLSLGGLG ERVLGFCQLY LNEKDYPPGY AFDVEAMNFP SSGLCFAGLV 610 620 630 640 650 660 SMIDPPRATV PDAVLKCRTA GIRVIMVTGD HPITAKAIAA SVGIISEGSE TVEDIAARLR 670 680 690 700 710 720 VPVDQVNRKD ARACVINGMQ LKDMDPSELV EALRTHPEMV FARTSPQQKL VIVESCQRLG 730 740 750 760 770 780 AIVAVTGDGV NDSPALKKAD IGVAMGIAGS DAAKNAADMI LLDDNFASIV TGVEQGRLIF 790 800 810 820 830 840 DNLKKSIAYT LTKNIPELTP YLIYITVSVP LPLGCITILF IELCTDIFPS VSLAYEKAES 850 860 870 880 890 900 DIMHLRPRNP KRDRLVNEPL AAYSYFQIGA IQSFAGFTDY FTAMAQEGWF PLLCVGLRAQ 910 920 930 940 950 960 WEDHHLQDLQ DSYGQEWTFG QRLYQQYTCY TVFFISIEVC QIADVLIRKT RRLSAFQQGF 970 980 990 1000 1010 1020 FRNKILVIAI VFQVCIGCFL CYCPGMPNIF NFMPIRFQWW LVPLPYGILI FVYDEIRKLG 1030 VRCCPGSWWD QELYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human gastric (H+ + K+)-ATPase gene. Similarity to (Na+ + K+)-ATPase genes in exon/intron organization but difference in control region." Maeda M., Oshiman K., Tamura S., Futai M. J. Biol. Chem. 265:9027-9032(1990) [PubMed: 2160952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-265.
[2]	"Structure of the human gastric H,K-ATPase gene and comparison of the 5'-flanking sequences of the human and rat genes." Newman P.R., Greeb J., Keeton T.P., Reyes A.A., Shull G.E. DNA Cell Biol. 9:749-762(1990) [PubMed: 2176086] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The family of human Na+,K+-ATPase genes. No less than five genes and/or pseudogenes related to the alpha-subunit." Sverdlov E.D., Monastyrskaya G.S., Broude N.E., Ushkaryov Y.A., Allikmets R.L., Melkov A.M., Smirnov Y.V., Malyshev I.V., Dulubova I.E., Petrukhin K.E., Gryshin A.V., Kiyatkin N.I., Kostina M.B., Sverdlov V.E., Modyanov N.N., Ovchinnikov Y.A. FEBS Lett. 217:275-278(1987) [PubMed: 3036582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 355-401. Tissue: Brain and Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J05451 Genomic DNA. Translation: AAA51010.1. M63962 Genomic DNA. Translation: AAA35988.1. AD000090 Genomic DNA. Translation: AAB50172.1. AC002389 Genomic DNA. Translation: AAB64182.1. M27575 Genomic DNA. Translation: AAA35577.1.
IPI	IPI00218919.
PIR	A35292. A36558. C27397.
RefSeq	NP_000695.2.
UniGene	Hs.36992
3D structure databases
HSSP	HSSP built from PDB template 1IWC based on UniProtKB P19156.
ModBase	Search...
Protein-protein interaction databases
STRING	P20648.
Protein family/group databases
TCDB	3.A.3.1.2. P-type ATPase (P-ATPase) superfamily.
PTM databases
PhosphoSite	P20648.
Proteomic databases
PRIDE	P20648.
Genome annotation databases
Ensembl	ENST00000262623; ENSP00000262623; ENSG00000105675; Homo sapiens. [Genome view]
GeneID	495.
KEGG	hsa:495.
UCSC	uc002oal.1. human.
Organism-specific databases
CTD	495.
GeneCards	GC19M040732.
HGNC	HGNC:819. ATP4A.
MIM	137216. gene.
PharmGKB	PA25113.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P20648.
HOVERGEN	P20648.
OMA	FQLSIHT.
Enzyme and pathway databases
BRENDA	3.6.3.10. 247.
Gene expression databases
ArrayExpress	P20648.
Bgee	P20648.
CleanEx	HS_ATP4A.
Genevestigator	P20648.
GermOnline	ENSG00000105675. Homo sapiens.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR005775. ATPase_P-typ_cation-ex_asu_euk. IPR006069. ATPase_P-typ_cation-exchng_asu. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR015127. ATPase_P-typ_H/K-transp_N. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF09040. H-K_ATPase_N. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00121. NAKATPASE.
TIGRFAMs	TIGR01106. ATPase-IIC_X-K. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00736. Esomeprazole. DB00448. Lansoprazole. DB00338. Omeprazole. DB00213. Pantoprazole. DB01129. Rabeprazole. DB00831. Trifluoperazine.
NextBio	2079.
SOURCE	Search...

Entry information

Entry name

ATP4A_HUMAN

Accession

Primary (citable) accession number: P20648
Secondary accession number(s): O00738

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 109 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.