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P20648 (ATP4A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 152. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium-transporting ATPase alpha chain 1

EC=3.6.3.10
Alternative name(s):
Gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene names
Name:ATP4A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1035 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for acid production in the stomach.

Catalytic activity

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Subunit structure

Composed of two subunits: alpha (catalytic) and beta.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Found in gastric mucosa.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. [View classification]

Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Potassium transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP biosynthetic process

Inferred from electronic annotation. Source: InterPro

ATP hydrolysis coupled proton transport

Inferred from electronic annotation. Source: InterPro

ion transmembrane transport

Traceable author statement. Source: Reactome

pH reduction

Inferred from electronic annotation. Source: Ensembl

regulation of proton transport

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

transmembrane transport

Traceable author statement. Source: Reactome

transport

Traceable author statement Ref.1. Source: ProtInc

   Cellular_componentextracellular space

Inferred from direct assay PubMed 22664934. Source: UniProt

integral component of plasma membrane

Traceable author statement Ref.1. Source: ProtInc

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

hydrogen:potassium-exchanging ATPase activity

Traceable author statement Ref.1. Source: ProtInc

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 10351034Potassium-transporting ATPase alpha chain 1
PRO_0000046253

Regions

Topological domain2 – 9897Cytoplasmic Potential
Transmembrane99 – 11921Helical; Potential
Topological domain120 – 14223Lumenal Potential
Transmembrane143 – 16321Helical; Potential
Topological domain164 – 299136Cytoplasmic Potential
Transmembrane300 – 31920Helical; Potential
Topological domain320 – 33112Lumenal Potential
Transmembrane332 – 34918Helical; Potential
Topological domain350 – 783434Cytoplasmic Potential
Transmembrane784 – 80320Helical; Potential
Topological domain804 – 81310Lumenal Potential
Transmembrane814 – 83421Helical; Potential
Topological domain835 – 85420Cytoplasmic Potential
Transmembrane855 – 87723Helical; Potential
Topological domain878 – 92952Lumenal Potential
Transmembrane930 – 94920Helical; Potential
Topological domain950 – 96314Cytoplasmic Potential
Transmembrane964 – 98219Helical; Potential
Topological domain983 – 99715Lumenal Potential
Transmembrane998 – 101821Helical; Potential
Topological domain1019 – 103517Cytoplasmic Potential

Sites

Active site38714-aspartylphosphate intermediate By similarity
Metal binding7281Magnesium By similarity
Metal binding7321Magnesium By similarity

Amino acid modifications

Modified residue71Phosphotyrosine By similarity
Modified residue101Phosphotyrosine By similarity
Modified residue271Phosphoserine By similarity
Modified residue9541Phosphoserine; by PKA By similarity

Natural variations

Natural variant2651V → A. Ref.1
Corresponds to variant rs2733743 [ dbSNP | Ensembl ].
VAR_019428

Sequences

Sequence LengthMass (Da)Tools
P20648 [UniParc].

Last modified May 29, 2007. Version 5.
Checksum: E320595E7D9E0E28

FASTA1,035114,119
        10         20         30         40         50         60 
MGKAENYELY SVELGPGPGG DMAAKMSKKK KAGGGGGKRK EKLENMKKEM EINDHQLSVA 

        70         80         90        100        110        120 
ELEQKYQTSA TKGLSASLAA ELLLRDGPNA LRPPRGTPEY VKFARQLAGG LQCLMWVAAA 

       130        140        150        160        170        180 
ICLIAFAIQA SEGDLTTDDN LYLAIALIAV VVVTGCFGYY QEFKSTNIIA SFKNLVPQQA 

       190        200        210        220        230        240 
TVIRDGDKFQ INADQLVVGD LVEMKGGDRV PADIRILAAQ GCKVDNSSLT GESEPQTRSP 

       250        260        270        280        290        300 
ECTHESPLET RNIAFFSTMC LEGTVQGLVV NTGDRTIIGR IASLASGVEN EKTPIAIEIE 

       310        320        330        340        350        360 
HFVDIIAGLA ILFGATFFIV AMCIGYTFLR AMVFFMAIVV AYVPEGLLAT VTVCLSLTAK 

       370        380        390        400        410        420 
RLASKNCVVK NLEAVETLGS TSVICSDKTG TLTQNRMTVS HLWFDNHIHT ADTTEDQSGQ 

       430        440        450        460        470        480 
TFDQSSETWR ALCRVLTLCN RAAFKSGQDA VPVPKRIVIG DASETALLKF SELTLGNAMG 

       490        500        510        520        530        540 
YRDRFPKVCE IPFNSTNKFQ LSIHTLEDPR DPRHLLVMKG APERVLERCS SILIKGQELP 

       550        560        570        580        590        600 
LDEQWREAFQ TAYLSLGGLG ERVLGFCQLY LNEKDYPPGY AFDVEAMNFP SSGLCFAGLV 

       610        620        630        640        650        660 
SMIDPPRATV PDAVLKCRTA GIRVIMVTGD HPITAKAIAA SVGIISEGSE TVEDIAARLR 

       670        680        690        700        710        720 
VPVDQVNRKD ARACVINGMQ LKDMDPSELV EALRTHPEMV FARTSPQQKL VIVESCQRLG 

       730        740        750        760        770        780 
AIVAVTGDGV NDSPALKKAD IGVAMGIAGS DAAKNAADMI LLDDNFASIV TGVEQGRLIF 

       790        800        810        820        830        840 
DNLKKSIAYT LTKNIPELTP YLIYITVSVP LPLGCITILF IELCTDIFPS VSLAYEKAES 

       850        860        870        880        890        900 
DIMHLRPRNP KRDRLVNEPL AAYSYFQIGA IQSFAGFTDY FTAMAQEGWF PLLCVGLRAQ 

       910        920        930        940        950        960 
WEDHHLQDLQ DSYGQEWTFG QRLYQQYTCY TVFFISIEVC QIADVLIRKT RRLSAFQQGF 

       970        980        990       1000       1010       1020 
FRNKILVIAI VFQVCIGCFL CYCPGMPNIF NFMPIRFQWW LVPLPYGILI FVYDEIRKLG 

      1030 
VRCCPGSWWD QELYY 

« Hide

References

« Hide 'large scale' references
[1]"Human gastric (H+ + K+)-ATPase gene. Similarity to (Na+ + K+)-ATPase genes in exon/intron organization but difference in control region."
Maeda M., Oshiman K., Tamura S., Futai M.
J. Biol. Chem. 265:9027-9032(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-265.
[2]"Structure of the human gastric H,K-ATPase gene and comparison of the 5'-flanking sequences of the human and rat genes."
Newman P.R., Greeb J., Keeton T.P., Reyes A.A., Shull G.E.
DNA Cell Biol. 9:749-762(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The family of human Na+,K+-ATPase genes. No less than five genes and/or pseudogenes related to the alpha-subunit."
Sverdlov E.D., Monastyrskaya G.S., Broude N.E., Ushkaryov Y.A., Allikmets R.L., Melkov A.M., Smirnov Y.V., Malyshev I.V., Dulubova I.E., Petrukhin K.E., Gryshin A.V., Kiyatkin N.I., Kostina M.B., Sverdlov V.E., Modyanov N.N., Ovchinnikov Y.A.
FEBS Lett. 217:275-278(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 355-401.
Tissue: Brain and Placenta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05451 Genomic DNA. Translation: AAA51010.1.
M63962 Genomic DNA. Translation: AAA35988.1.
AD000090 Genomic DNA. Translation: AAB50172.1.
AC002389 Genomic DNA. Translation: AAB64182.1.
M27575 Genomic DNA. Translation: AAA35577.1.
PIRA35292. A36558.
C27397.
RefSeqNP_000695.2. NM_000704.2.
UniGeneHs.36992.

3D structure databases

ProteinModelPortalP20648.
SMRP20648. Positions 1-34, 43-1035.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid106985. 76 interactions.
STRING9606.ENSP00000262623.

Chemistry

BindingDBP20648.
ChEMBLCHEMBL2095173.
DrugBankDB00736. Esomeprazole.
DB00448. Lansoprazole.
DB00338. Omeprazole.
DB00213. Pantoprazole.
DB01129. Rabeprazole.
DB00831. Trifluoperazine.

Protein family/group databases

TCDB3.A.3.1.2. the p-type atpase (p-atpase) superfamily.

PTM databases

PhosphoSiteP20648.

Polymorphism databases

DMDM148877240.

Proteomic databases

PaxDbP20648.
PRIDEP20648.

Protocols and materials databases

DNASU495.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000262623; ENSP00000262623; ENSG00000105675.
GeneID495.
KEGGhsa:495.
UCSCuc002oal.1. human.

Organism-specific databases

CTD495.
GeneCardsGC19M036540.
HGNCHGNC:819. ATP4A.
HPAHPA039154.
MIM137216. gene.
neXtProtNX_P20648.
PharmGKBPA25113.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0474.
HOGENOMHOG000265622.
HOVERGENHBG004298.
InParanoidP20648.
KOK01542.
OMARVIVICQ.
OrthoDBEOG7327N0.
PhylomeDBP20648.
TreeFamTF312838.

Enzyme and pathway databases

BioCycMetaCyc:HS02790-MONOMER.
BRENDA3.6.3.10. 2681.
ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

BgeeP20648.
CleanExHS_ATP4A.
GenevestigatorP20648.

Family and domain databases

Gene3D1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR015127. ATPase_P-typ_H/K-transp_N.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF09040. H-K_ATPase_N. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi495.
NextBio2079.
PROP20648.
SOURCESearch...

Entry information

Entry nameATP4A_HUMAN
AccessionPrimary (citable) accession number: P20648
Secondary accession number(s): O00738
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 29, 2007
Last modified: April 16, 2014
This is version 152 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM