Reviewed, UniProtKB/Swiss-Prot P20645 (MPRD_HUMAN)

Names and origin

Protein names

Recommended name:
    Cation-dependent mannose-6-phosphate receptor
      Short name=CD Man-6-P receptor
      Short name=CD-MPR
Alternative name(s):
    46 kDa mannose 6-phosphate receptor
    MPR 46

Gene names

Name:	M6PR
Synonyms:	MPR46, MPRD

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
Subunit structure	Homodimer. Binds GGA1, GGA2 and GGA3.
Subcellular location	Lysosome membrane; Single-pass type I membrane protein.
Domain	The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the cation-independent mannose 6-phosphate receptor.
Miscellaneous	This receptor has optimal binding in the presence of divalent cations.

Ontologies

Keywords
Biological process	Transport
Cellular component	Lysosome Membrane
Domain	Signal Transmembrane
Molecular function	Receptor
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	endosome to lysosome transport Traceable author statement. Source: ProtInc mannose transport Inferred from electronic annotation. Source: InterPro receptor-mediated endocytosis Traceable author statement. Source: ProtInc
Cellular component	cell surface Inferred from direct assay. Source: UniProtKB integral to plasma membrane Traceable author statement. Source: ProtInc lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannose binding Inferred from electronic annotation. Source: InterPro mannose transmembrane transporter activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: InterPro transmembrane receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26
	Chain	27 – 277	251	Cation-dependent mannose-6-phosphate receptor		PRO_0000019226
Regions
	Topological domain	27 – 185	159	Lumenal Potential
	Transmembrane	186 – 210	25	Potential
	Topological domain	211 – 277	67	Cytoplasmic Potential
Amino acid modifications
	Modified residue	267	1	Phosphoserine Ref.6
	Glycosylation	57	1	N-linked (GlcNAc...) Potential
	Glycosylation	83	1	N-linked (GlcNAc...)
	Glycosylation	94	1	N-linked (GlcNAc...) Potential
	Glycosylation	107	1	N-linked (GlcNAc...)
	Glycosylation	113	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P20645-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 1093FE25DEB6C8E8	FASTA	277	30,993
	10 20 30 40 50 60 MFPFYSCWRT GLLLLLLAVA VRESWQTEEK TCDLVGEKGK ESEKELALVK RLKPLFNKSF 70 80 90 100 110 120 ESTVGQGSDT YIYIFRVCRE AGNHTSGAGL VQINKSNGKE TVVGRLNETH IFNGSNWIML 130 140 150 160 170 180 IYKGGDEYDN HCGKEQRRAV VMISCNRHTL ADNFNPVSEE RGKVQDCFYL FEMDSSLACS 190 200 210 220 230 240 PEISHLSVGS ILLVTFASLV AVYVVGGFLY QRLVVGAKGM EQFPHLAFWQ DLGNLVADGC 250 260 270 DFVCRSKPRN VPAAYRGVGD DQLGEESEER DDHLLPM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a cDNA encoding the human cation-dependent mannose 6-phosphate-specific receptor." Pohlmann R., Nagel G., Schmidt B., Stein M., Lorkowski G., Krentler C., Cully J., Meyer H.E., Grzeschik K.H., Mersmann G., Hasilik A., von Figura K. Proc. Natl. Acad. Sci. U.S.A. 84:5575-5579(1987) [PubMed: 2441386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation and analysis of the human 46-kDa mannose 6-phosphate receptor gene." Klier H.J., von Figura K., Pohlmann R. Eur. J. Biochem. 197:23-28(1991) [PubMed: 1849818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Leukocyte.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[4]	"Sorting of mannose 6-phosphate receptors mediated by the GGAs." Puertollano R., Aguilar R.C., Gorshkova I., Crouch R.J., Bonifacino J.S. Science 292:1712-1716(2001) [PubMed: 11387475] [Abstract] Cited for: INTERACTION WITH GGA1 AND GGA3.
[5]	"Interaction of the cation-dependent mannose 6-phosphate receptor with GGA proteins." Doray B., Bruns K., Ghosh P., Kornfeld S. J. Biol. Chem. 277:18477-18482(2002) [PubMed: 11886874] [Abstract] Cited for: INTERACTION WITH GGA2.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-267, MASS SPECTROMETRY.
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[8]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-83 AND ASN-107, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M16985 mRNA. Translation: AAA59542.1. X56254 , X56255, X56256, X56257 Genomic DNA. Translation: CAB94715.1. BC024206 mRNA. Translation: AAH24206.1.
IPI	IPI00025049.
PIR	A32700. S15368.
RefSeq	NP_002346.1.
UniGene	Hs.134084
3D structure databases
HSSP	HSSP built from PDB template 1M6P based on UniProtKB P11456.
SMR	P20645. Positions 29-180.
ModBase	Search...
PTM databases
PhosphoSite	P20645.
Proteomic databases
PeptideAtlas	P20645.
PRIDE	P20645.
Genome annotation databases
Ensembl	ENSG00000003056. Homo sapiens. [Contig view]
GeneID	4074.
KEGG	hsa:4074.
Organism-specific databases
GeneCards	GC12M008984.
H-InvDB	HIX0017826.
HGNC	HGNC:6752. M6PR.
MIM	154540. gene.
PharmGKB	PA30513.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P20645.
HOVERGEN	P20645.
OMA	P20645. NETHVFN.
Gene expression databases
Bgee	P20645.
CleanEx	HS_M6PR.
GermOnline	ENSG00000003056. Homo sapiens.
Family and domain databases
InterPro	IPR000296. Man_6_P_rcpt. [Graphical view]
Pfam	PF02157. Man-6-P_recep. 1 hit. [Graphical view]
PRINTS	PR00715. MAN6PRECEPTR.
ProtoNet	Search...
Other Resources
NextBio	15968.
SOURCE	Search...

Entry information

Entry name

MPRD_HUMAN

Accession

Primary (citable) accession number: P20645

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)