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P20645 (MPRD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cation-dependent mannose-6-phosphate receptor

Short name=CD Man-6-P receptor
Short name=CD-MPR
Alternative name(s):
46 kDa mannose 6-phosphate receptor
Short name=MPR 46
Gene names
Name:M6PR
Synonyms:MPR46, MPRD
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length277 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.

Subunit structure

Homodimer. Binds GGA1, GGA2 and GGA3.

Subcellular location

Lysosome membrane; Single-pass type I membrane protein.

Domain

The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the cation-independent mannose 6-phosphate receptor.

Miscellaneous

This receptor has optimal binding in the presence of divalent cations.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626
Chain27 – 277251Cation-dependent mannose-6-phosphate receptor
PRO_0000019226

Regions

Topological domain27 – 185159Lumenal Potential
Transmembrane186 – 21025Helical; Potential
Topological domain211 – 27767Cytoplasmic Potential

Amino acid modifications

Modified residue2671Phosphoserine By similarity
Glycosylation571N-linked (GlcNAc...) Potential
Glycosylation831N-linked (GlcNAc...) Ref.9
Glycosylation941N-linked (GlcNAc...) Potential
Glycosylation1071N-linked (GlcNAc...) Ref.9
Glycosylation1131N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P20645 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 1093FE25DEB6C8E8

FASTA27730,993
        10         20         30         40         50         60 
MFPFYSCWRT GLLLLLLAVA VRESWQTEEK TCDLVGEKGK ESEKELALVK RLKPLFNKSF 

        70         80         90        100        110        120 
ESTVGQGSDT YIYIFRVCRE AGNHTSGAGL VQINKSNGKE TVVGRLNETH IFNGSNWIML 

       130        140        150        160        170        180 
IYKGGDEYDN HCGKEQRRAV VMISCNRHTL ADNFNPVSEE RGKVQDCFYL FEMDSSLACS 

       190        200        210        220        230        240 
PEISHLSVGS ILLVTFASLV AVYVVGGFLY QRLVVGAKGM EQFPHLAFWQ DLGNLVADGC 

       250        260        270 
DFVCRSKPRN VPAAYRGVGD DQLGEESEER DDHLLPM 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a cDNA encoding the human cation-dependent mannose 6-phosphate-specific receptor."
Pohlmann R., Nagel G., Schmidt B., Stein M., Lorkowski G., Krentler C., Cully J., Meyer H.E., Grzeschik K.H., Mersmann G., Hasilik A., von Figura K.
Proc. Natl. Acad. Sci. U.S.A. 84:5575-5579(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Isolation and analysis of the human 46-kDa mannose 6-phosphate receptor gene."
Klier H.J., von Figura K., Pohlmann R.
Eur. J. Biochem. 197:23-28(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Leukocyte.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Muscle.
[6]"Sorting of mannose 6-phosphate receptors mediated by the GGAs."
Puertollano R., Aguilar R.C., Gorshkova I., Crouch R.J., Bonifacino J.S.
Science 292:1712-1716(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GGA1 AND GGA3.
[7]"Interaction of the cation-dependent mannose 6-phosphate receptor with GGA proteins."
Doray B., Bruns K., Ghosh P., Kornfeld S.
J. Biol. Chem. 277:18477-18482(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GGA2.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-83 AND ASN-107.
Tissue: Liver.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16985 mRNA. Translation: AAA59542.1.
X56254 expand/collapse EMBL AC list , X56255, X56256, X56257 Genomic DNA. Translation: CAB94715.1.
AK291143 mRNA. Translation: BAF83832.1.
CH471116 Genomic DNA. Translation: EAW88596.1.
CH471116 Genomic DNA. Translation: EAW88597.1.
CH471116 Genomic DNA. Translation: EAW88598.1.
CH471116 Genomic DNA. Translation: EAW88599.1.
BC024206 mRNA. Translation: AAH24206.1.
CCDSCCDS8598.1.
PIRA32700. S15368.
RefSeqNP_002346.1. NM_002355.3.
XP_005253433.1. XM_005253376.1.
UniGeneHs.134084.
Hs.744902.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JUQX-ray2.20E/F/G/H265-277[»]
ProteinModelPortalP20645.
SMRP20645. Positions 27-180.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110251. 19 interactions.
IntActP20645. 3 interactions.
MINTMINT-4999970.
STRING9606.ENSP00000000412.

Chemistry

BindingDBP20645.
ChEMBLCHEMBL5788.

PTM databases

PhosphoSiteP20645.

Polymorphism databases

DMDM127293.

Proteomic databases

MaxQBP20645.
PaxDbP20645.
PeptideAtlasP20645.
PRIDEP20645.

Protocols and materials databases

DNASU4074.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000000412; ENSP00000000412; ENSG00000003056.
GeneID4074.
KEGGhsa:4074.
UCSCuc001qvf.3. human.

Organism-specific databases

CTD4074.
GeneCardsGC12M008993.
HGNCHGNC:6752. M6PR.
HPACAB034464.
MIM154540. gene.
neXtProtNX_P20645.
PharmGKBPA30513.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG45234.
HOGENOMHOG000013085.
HOVERGENHBG006395.
InParanoidP20645.
KOK10089.
OMASDWIMLI.
PhylomeDBP20645.
TreeFamTF328910.

Enzyme and pathway databases

ReactomeREACT_11123. Membrane Trafficking.

Gene expression databases

ArrayExpressP20645.
BgeeP20645.
CleanExHS_M6PR.
GenevestigatorP20645.

Family and domain databases

Gene3D2.70.130.10. 1 hit.
InterProIPR028927. Man-6-P_rcpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR000296. Man_6_P_rcpt.
[Graphical view]
PfamPF02157. Man-6-P_recep. 1 hit.
[Graphical view]
PRINTSPR00715. MAN6PRECEPTR.
SUPFAMSSF50911. SSF50911. 1 hit.
ProtoNetSearch...

Other

GeneWikiCation-dependent_mannose-6-phosphate_receptor.
GenomeRNAi4074.
NextBio15968.
PROP20645.
SOURCESearch...

Entry information

Entry nameMPRD_HUMAN
AccessionPrimary (citable) accession number: P20645
Secondary accession number(s): A8K528, D3DUV5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM