P20645 (MPRD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cation-dependent mannose-6-phosphate receptor
Short name=CD Man-6-P receptor
Short name=CD-MPR

Alternative name(s):
46 kDa mannose 6-phosphate receptor
Short name=MPR 46

Gene names

Name:	M6PR
Synonyms:	MPR46, MPRD

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
Subunit structure	Homodimer. Binds GGA1, GGA2 and GGA3.
Subcellular location	Lysosome membrane; Single-pass type I membrane protein.
Domain	The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the cation-independent mannose 6-phosphate receptor.
Miscellaneous	This receptor has optimal binding in the presence of divalent cations.

Ontologies

Keywords
Biological process	Transport
Cellular component	Lysosome Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endosome to lysosome transport Traceable author statement. Source: ProtInc receptor-mediated endocytosis Traceable author statement. Source: ProtInc
Cellular component	cell surface Inferred from direct assay. Source: UniProtKB integral to plasma membrane Traceable author statement. Source: ProtInc lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannose binding Inferred from electronic annotation. Source: InterPro mannose transmembrane transporter activity Inferred from electronic annotation. Source: InterPro transmembrane signaling receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26
	Chain	27 – 277	251	Cation-dependent mannose-6-phosphate receptor		PRO_0000019226
Regions
	Topological domain	27 – 185	159	Lumenal Potential
	Transmembrane	186 – 210	25	Helical; Potential
	Topological domain	211 – 277	67	Cytoplasmic Potential
Amino acid modifications
	Modified residue	267	1	Phosphoserine Ref.8
	Glycosylation	57	1	N-linked (GlcNAc...) Potential
	Glycosylation	83	1	N-linked (GlcNAc...) Ref.9
	Glycosylation	94	1	N-linked (GlcNAc...) Potential
	Glycosylation	107	1	N-linked (GlcNAc...) Ref.9
	Glycosylation	113	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P20645 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 1093FE25DEB6C8E8	FASTA	277	30,993
	10 20 30 40 50 60 MFPFYSCWRT GLLLLLLAVA VRESWQTEEK TCDLVGEKGK ESEKELALVK RLKPLFNKSF 70 80 90 100 110 120 ESTVGQGSDT YIYIFRVCRE AGNHTSGAGL VQINKSNGKE TVVGRLNETH IFNGSNWIML 130 140 150 160 170 180 IYKGGDEYDN HCGKEQRRAV VMISCNRHTL ADNFNPVSEE RGKVQDCFYL FEMDSSLACS 190 200 210 220 230 240 PEISHLSVGS ILLVTFASLV AVYVVGGFLY QRLVVGAKGM EQFPHLAFWQ DLGNLVADGC 250 260 270 DFVCRSKPRN VPAAYRGVGD DQLGEESEER DDHLLPM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a cDNA encoding the human cation-dependent mannose 6-phosphate-specific receptor." Pohlmann R., Nagel G., Schmidt B., Stein M., Lorkowski G., Krentler C., Cully J., Meyer H.E., Grzeschik K.H., Mersmann G., Hasilik A., von Figura K. Proc. Natl. Acad. Sci. U.S.A. 84:5575-5579(1987) [PubMed: 2441386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation and analysis of the human 46-kDa mannose 6-phosphate receptor gene." Klier H.J., von Figura K., Pohlmann R. Eur. J. Biochem. 197:23-28(1991) [PubMed: 1849818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Leukocyte.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[6]	"Sorting of mannose 6-phosphate receptors mediated by the GGAs." Puertollano R., Aguilar R.C., Gorshkova I., Crouch R.J., Bonifacino J.S. Science 292:1712-1716(2001) [PubMed: 11387475] [Abstract] Cited for: INTERACTION WITH GGA1 AND GGA3.
[7]	"Interaction of the cation-dependent mannose 6-phosphate receptor with GGA proteins." Doray B., Bruns K., Ghosh P., Kornfeld S. J. Biol. Chem. 277:18477-18482(2002) [PubMed: 11886874] [Abstract] Cited for: INTERACTION WITH GGA2.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-267, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-83 AND ASN-107, MASS SPECTROMETRY. Tissue: Liver.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M16985 mRNA. Translation: AAA59542.1. X56254 , X56255, X56256, X56257 Genomic DNA. Translation: CAB94715.1. AK291143 mRNA. Translation: BAF83832.1. CH471116 Genomic DNA. Translation: EAW88596.1. CH471116 Genomic DNA. Translation: EAW88597.1. CH471116 Genomic DNA. Translation: EAW88598.1. CH471116 Genomic DNA. Translation: EAW88599.1. BC024206 mRNA. Translation: AAH24206.1.
IPI	IPI00025049.
PIR	A32700. S15368.
RefSeq	NP_002346.1. NM_002355.3.
UniGene	Hs.134084. Hs.305985.
3D structure databases
ProteinModelPortal	P20645.
SMR	P20645. Positions 27-180.
ModBase	Search...
Protein-protein interaction databases
IntAct	P20645. 1 interaction.
MINT	MINT-4999970.
STRING	P20645.
PTM databases
PhosphoSite	P20645.
Polymorphism databases
DMDM	127293.
Proteomic databases
PeptideAtlas	P20645.
PRIDE	P20645.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000000412; ENSP00000000412; ENSG00000003056.
GeneID	4074.
KEGG	hsa:4074.
UCSC	uc001qvf.1. human.
Organism-specific databases
CTD	4074.
GeneCards	GC12M008993.
H-InvDB	HIX0017826.
HGNC	HGNC:6752. M6PR.
HPA	CAB034464.
MIM	154540. gene.
neXtProt	NX_P20645.
PharmGKB	PA30513.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19685.
HOGENOM	HBG268550.
HOVERGEN	HBG006395.
InParanoid	P20645.
OMA	NETHVFN.
OrthoDB	EOG43FGXF.
PhylomeDB	P20645.
Enzyme and pathway databases
Reactome	REACT_11123. Membrane Trafficking.
Gene expression databases
ArrayExpress	P20645.
Bgee	P20645.
CleanEx	HS_M6PR.
Genevestigator	P20645.
GermOnline	ENSG00000003056. Homo sapiens.
Family and domain databases
InterPro	IPR009011. Man6P_isomerase_rcpt-bd. IPR000296. Man_6_P_rcpt. [Graphical view]
KO	K10089.
PRINTS	PR00715. MAN6PRECEPTR.
SUPFAM	SSF50911. Man6php_recept. 1 hit.
ProtoNet	Search...
Other
NextBio	15968.
SOURCE	Search...

Entry information

Entry name

MPRD_HUMAN

Accession

Primary (citable) accession number: P20645
Secondary accession number(s): A8K528, D3DUV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	January 25, 2012
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.