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P20645

- MPRD_HUMAN

UniProt

P20645 - MPRD_HUMAN

Protein

Cation-dependent mannose-6-phosphate receptor

Gene

M6PR

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.

    GO - Molecular functioni

    1. mannose binding Source: InterPro
    2. mannose transmembrane transporter activity Source: InterPro
    3. transmembrane signaling receptor activity Source: ProtInc

    GO - Biological processi

    1. endosome to lysosome transport Source: ProtInc
    2. receptor-mediated endocytosis Source: ProtInc
    3. signal transduction Source: GOC

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_19287. Lysosome Vesicle Biogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cation-dependent mannose-6-phosphate receptor
    Short name:
    CD Man-6-P receptor
    Short name:
    CD-MPR
    Alternative name(s):
    46 kDa mannose 6-phosphate receptor
    Short name:
    MPR 46
    Gene namesi
    Name:M6PR
    Synonyms:MPR46, MPRD
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:6752. M6PR.

    Subcellular locationi

    GO - Cellular componenti

    1. endosome Source: MGI
    2. integral component of plasma membrane Source: ProtInc
    3. late endosome Source: Ensembl
    4. lysosomal membrane Source: UniProtKB-SubCell
    5. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Lysosome, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30513.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Add
    BLAST
    Chaini27 – 277251Cation-dependent mannose-6-phosphate receptorPRO_0000019226Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi83 – 831N-linked (GlcNAc...)1 Publication
    Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi107 – 1071N-linked (GlcNAc...)1 Publication
    Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
    Modified residuei267 – 2671PhosphoserineBy similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP20645.
    PaxDbiP20645.
    PeptideAtlasiP20645.
    PRIDEiP20645.

    PTM databases

    PhosphoSiteiP20645.

    Expressioni

    Gene expression databases

    ArrayExpressiP20645.
    BgeeiP20645.
    CleanExiHS_M6PR.
    GenevestigatoriP20645.

    Organism-specific databases

    HPAiCAB034464.

    Interactioni

    Subunit structurei

    Homodimer. Binds GGA1, GGA2 and GGA3.

    Protein-protein interaction databases

    BioGridi110251. 19 interactions.
    IntActiP20645. 5 interactions.
    MINTiMINT-4999970.
    STRINGi9606.ENSP00000000412.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JUQX-ray2.20E/F/G/H265-277[»]
    ProteinModelPortaliP20645.
    SMRiP20645. Positions 27-180.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini27 – 185159LumenalSequence AnalysisAdd
    BLAST
    Topological domaini211 – 27767CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei186 – 21025HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the cation-independent mannose 6-phosphate receptor.

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG45234.
    HOGENOMiHOG000013085.
    HOVERGENiHBG006395.
    InParanoidiP20645.
    KOiK10089.
    OMAiSDWIMLI.
    PhylomeDBiP20645.
    TreeFamiTF328910.

    Family and domain databases

    Gene3Di2.70.130.10. 1 hit.
    InterProiIPR028927. Man-6-P_rcpt.
    IPR009011. Man6P_isomerase_rcpt-bd_dom.
    IPR000296. Man_6_P_rcpt.
    [Graphical view]
    PfamiPF02157. Man-6-P_recep. 1 hit.
    [Graphical view]
    PRINTSiPR00715. MAN6PRECEPTR.
    SUPFAMiSSF50911. SSF50911. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P20645-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFPFYSCWRT GLLLLLLAVA VRESWQTEEK TCDLVGEKGK ESEKELALVK    50
    RLKPLFNKSF ESTVGQGSDT YIYIFRVCRE AGNHTSGAGL VQINKSNGKE 100
    TVVGRLNETH IFNGSNWIML IYKGGDEYDN HCGKEQRRAV VMISCNRHTL 150
    ADNFNPVSEE RGKVQDCFYL FEMDSSLACS PEISHLSVGS ILLVTFASLV 200
    AVYVVGGFLY QRLVVGAKGM EQFPHLAFWQ DLGNLVADGC DFVCRSKPRN 250
    VPAAYRGVGD DQLGEESEER DDHLLPM 277
    Length:277
    Mass (Da):30,993
    Last modified:February 1, 1991 - v1
    Checksum:i1093FE25DEB6C8E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16985 mRNA. Translation: AAA59542.1.
    X56254
    , X56255, X56256, X56257 Genomic DNA. Translation: CAB94715.1.
    AK291143 mRNA. Translation: BAF83832.1.
    CH471116 Genomic DNA. Translation: EAW88596.1.
    CH471116 Genomic DNA. Translation: EAW88597.1.
    CH471116 Genomic DNA. Translation: EAW88598.1.
    CH471116 Genomic DNA. Translation: EAW88599.1.
    BC024206 mRNA. Translation: AAH24206.1.
    CCDSiCCDS8598.1.
    PIRiS15368. A32700.
    RefSeqiNP_002346.1. NM_002355.3.
    XP_005253433.1. XM_005253376.1.
    UniGeneiHs.134084.
    Hs.744902.

    Genome annotation databases

    EnsembliENST00000000412; ENSP00000000412; ENSG00000003056.
    GeneIDi4074.
    KEGGihsa:4074.
    UCSCiuc001qvf.3. human.

    Polymorphism databases

    DMDMi127293.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16985 mRNA. Translation: AAA59542.1 .
    X56254
    , X56255 , X56256 , X56257 Genomic DNA. Translation: CAB94715.1 .
    AK291143 mRNA. Translation: BAF83832.1 .
    CH471116 Genomic DNA. Translation: EAW88596.1 .
    CH471116 Genomic DNA. Translation: EAW88597.1 .
    CH471116 Genomic DNA. Translation: EAW88598.1 .
    CH471116 Genomic DNA. Translation: EAW88599.1 .
    BC024206 mRNA. Translation: AAH24206.1 .
    CCDSi CCDS8598.1.
    PIRi S15368. A32700.
    RefSeqi NP_002346.1. NM_002355.3.
    XP_005253433.1. XM_005253376.1.
    UniGenei Hs.134084.
    Hs.744902.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JUQ X-ray 2.20 E/F/G/H 265-277 [» ]
    ProteinModelPortali P20645.
    SMRi P20645. Positions 27-180.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110251. 19 interactions.
    IntActi P20645. 5 interactions.
    MINTi MINT-4999970.
    STRINGi 9606.ENSP00000000412.

    Chemistry

    BindingDBi P20645.
    ChEMBLi CHEMBL5788.

    PTM databases

    PhosphoSitei P20645.

    Polymorphism databases

    DMDMi 127293.

    Proteomic databases

    MaxQBi P20645.
    PaxDbi P20645.
    PeptideAtlasi P20645.
    PRIDEi P20645.

    Protocols and materials databases

    DNASUi 4074.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000000412 ; ENSP00000000412 ; ENSG00000003056 .
    GeneIDi 4074.
    KEGGi hsa:4074.
    UCSCi uc001qvf.3. human.

    Organism-specific databases

    CTDi 4074.
    GeneCardsi GC12M008993.
    HGNCi HGNC:6752. M6PR.
    HPAi CAB034464.
    MIMi 154540. gene.
    neXtProti NX_P20645.
    PharmGKBi PA30513.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG45234.
    HOGENOMi HOG000013085.
    HOVERGENi HBG006395.
    InParanoidi P20645.
    KOi K10089.
    OMAi SDWIMLI.
    PhylomeDBi P20645.
    TreeFami TF328910.

    Enzyme and pathway databases

    Reactomei REACT_19287. Lysosome Vesicle Biogenesis.

    Miscellaneous databases

    GeneWikii Cation-dependent_mannose-6-phosphate_receptor.
    GenomeRNAii 4074.
    NextBioi 15968.
    PROi P20645.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P20645.
    Bgeei P20645.
    CleanExi HS_M6PR.
    Genevestigatori P20645.

    Family and domain databases

    Gene3Di 2.70.130.10. 1 hit.
    InterProi IPR028927. Man-6-P_rcpt.
    IPR009011. Man6P_isomerase_rcpt-bd_dom.
    IPR000296. Man_6_P_rcpt.
    [Graphical view ]
    Pfami PF02157. Man-6-P_recep. 1 hit.
    [Graphical view ]
    PRINTSi PR00715. MAN6PRECEPTR.
    SUPFAMi SSF50911. SSF50911. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation and analysis of the human 46-kDa mannose 6-phosphate receptor gene."
      Klier H.J., von Figura K., Pohlmann R.
      Eur. J. Biochem. 197:23-28(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Leukocyte.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Muscle.
    6. "Sorting of mannose 6-phosphate receptors mediated by the GGAs."
      Puertollano R., Aguilar R.C., Gorshkova I., Crouch R.J., Bonifacino J.S.
      Science 292:1712-1716(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GGA1 AND GGA3.
    7. "Interaction of the cation-dependent mannose 6-phosphate receptor with GGA proteins."
      Doray B., Bruns K., Ghosh P., Kornfeld S.
      J. Biol. Chem. 277:18477-18482(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GGA2.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
      J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-83 AND ASN-107.
      Tissue: Liver.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMPRD_HUMAN
    AccessioniPrimary (citable) accession number: P20645
    Secondary accession number(s): A8K528, D3DUV5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 142 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    This receptor has optimal binding in the presence of divalent cations.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3