P20626 (PLD_CORPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholipase D
Short name=PLD
EC=3.1.4.4

Alternative name(s):
Choline phosphatase

Gene names

Name:

pld

Organism

Corynebacterium pseudotuberculosis

Taxonomic identifier

1719 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Virulence factor affecting bacterial dissemination and survival within the host. Has magnesium-dependent sphingomyelinase and hemolytic activity.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.

Ontologies

Keywords
Biological process	Lipid degradation Virulence
Domain	Signal
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from mutant phenotype Ref.4. Source: UniProtKB sphingomyelin metabolic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	periplasmic space Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phospholipase D activity Inferred from electronic annotation. Source: EC sphingomyelin phosphodiesterase D activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.1
	Chain	25 – 307	283	Phospholipase D		PRO_0000022066
Sites
	Active site	44	1	Potential
Natural variations
	Natural variant	5 – 6	2	VV → FA in strain: Biovar equi / isolate 155.
	Natural variant	8	1	F → L in strain: Biovar equi / isolate 155.
	Natural variant	189	1	E → G in strain: Biovar equi / isolate 155.
	Natural variant	205	1	N → D in strain: Biovar equi / isolate 155.
	Natural variant	270	1	I → M in strain: Biovar equi / isolate 155.
	Natural variant	277	1	A → P in strain: Biovar equi / isolate 155.

Sequences

	Sequence		Length	Mass (Da)
	P20626 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: D3B1334E6FC99875	FASTA	307	33,884
	10 20 30 40 50 60 MREKVVLFLS IIMAIMLPVG NAAAAPVVHN PASTANRPVY AIAHRVLTTQ GVDDAVAIGA 70 80 90 100 110 120 NALEIDFTAW GRGWWADHDG IPTSAGATAE EIFKHIADKR KQGANITFTW LDIKNPDYCR 130 140 150 160 170 180 DARSVCSINA LRDLARKYLE PAGVRVLYGF YKTVGGPAWK TITADLRDGE AVALSGPAQD 190 200 210 220 230 240 VLNDFARSEN KILTKQKIAD YGYYNINQGF GNCYGTWNRT CDQLRKSSEA RDQGKLGKTF 250 260 270 280 290 300 GWTIATGQDA RVNDLLGKAN VDGLIFGFKI THFYRHADTE NSFKAIKRWV DKHSATHHLA TVADNPW

References

[1]	"Cloning, nucleotide sequence, and expression in Escherichia coli of the phospholipase D gene from Corynebacterium pseudotuberculosis." Hodgson A.L.M., Bird P., Nisbet I.T. J. Bacteriol. 172:1256-1261(1990) [PubMed: 2407718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-47.
[2]	"Cloning and expression of the phospholipase D gene from Corynebacterium pseudotuberculosis in Escherichia coli." Songer J.G., Libby S.J., Iandolo J.J., Cuevas W.A. Infect. Immun. 58:131-136(1990) [PubMed: 2403529] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Biovar ovis / Isolate Whetten 1.
[3]	"Toxic phospholipases D of Corynebacterium pseudotuberculosis, C. ulcerans and Arcanobacterium haemolyticum: cloning and sequence homology." McNamara P.J., Cuevas W.A., Songer J.G. Gene 156:113-118(1995) [PubMed: 7737503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Biovar equi / Isolate 155.
[4]	"Targeted mutagenesis of the phospholipase D gene results in decreased virulence of Corynebacterium pseudotuberculosis." McNamara P.J., Bradley G.A., Songer J.G. Mol. Microbiol. 12:921-930(1994) [PubMed: 7934899] [Abstract] Cited for: MUTAGENESIS. Strain: Biovar ovis / Isolate Whetten 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L16587 Genomic DNA. Translation: AAA64910.1. L16586 Genomic DNA. Translation: AAA99867.1.
PIR	A35125.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR016674. PLipase_D_bac/fun. [Graphical view]
Gene3D	G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit.
PIRSF	PIRSF016632. Phospholipase_actinobac/fun. 1 hit.
SUPFAM	SSF51695. PLC-like_Pdiesterase_TIM-brl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLD_CORPS

Accession

Primary (citable) accession number: P20626
Secondary accession number(s): Q59314

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 31, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program