Reviewed,
UniProtKB/Swiss-Prot P20618 (PSB1_HUMAN)
Last modified
July 7, 2009.
Version 105.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Proteasome subunit beta type-1 EC=3.4.25.1 Alternative name(s): Proteasome component C5 Macropain subunit C5 Multicatalytic endopeptidase complex subunit C5 Proteasome gamma chain | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 241 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. |
| Catalytic activity | Cleavage of peptide bonds with very broad specificity. |
| Subunit structure | The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. |
| Subcellular location | |
| Sequence similarities | Belongs to the peptidase T1B family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| CASP4 | P49662 | 1 | EBI-372273,EBI-1057327 | |
| DCP2 | Q8IU60 | 1 | EBI-372273,EBI-521577 | |
| DSTYK | Q6XUX3 | 1 | EBI-372273,EBI-1049520 | |
| PASK | Q96RG2 | 1 | EBI-372273,EBI-1042651 | |
| RIOK3 | O14730 | 1 | EBI-372273,EBI-1047061 | |
| VHL | P40337 | 1 | EBI-372273,EBI-301246 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Propeptide | 1 – 28 | 28 | Ref.6 | PRO_0000259623 | |||||
| Chain | 29 – 241 | 213 | Proteasome subunit beta type-1 | PRO_0000148030 | |||||
Amino acid modifications | |||||||||
| Modified residue | 150 | 1 | Phosphotyrosine By similarity | ||||||
Natural variations | |||||||||
| Natural variant | 11 | 1 | P → A: dbSNP rs12717. Ref.5 | VAR_051547 | |||||
| Natural variant | 208 | 1 | I → N: dbSNP rs10541. | VAR_051548 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning and sequence analysis of cDNAs for five major subunits of human proteasomes (multi-catalytic proteinase complexes)." Tamura T., Lee D.H., Osaka F., Fujiwara T., Shin S., Chung C.H., Tanaka K., Ichihara A. Biochim. Biophys. Acta 1089:95-102(1991) [PubMed: 2025653] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [3] | "Human protein factory for converting the transcriptome into an in vitro-expressed proteome." Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. Nomura N.Nat. Methods 5:1011-1017(2008) [PubMed: 19054851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | "The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. Beck S.Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-11. Tissue: Brain and Lung. |
| [6] | "Relationships among the subunits of the high molecular weight proteinase, macropain (proteasome)." Lee L.W., Moomaw C.R., Orth K., McGuire M.J., DeMartino G.N., Slaughter C.A. Biochim. Biophys. Acta 1037:178-185(1990) [PubMed: 2306472] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-52. |
| [7] | "Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: PROTEOLYTIC PROCESSING [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [8] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| D00761 mRNA. Translation: BAA00658.1. BT019720 mRNA. Translation: AAV38525.1. AB451312 mRNA. Translation: BAG70126.1. AB451442 mRNA. Translation: BAG70256.1. AL031259 Genomic DNA. Translation: CAA20287.1. BC000508 mRNA. Translation: AAH00508.1. BC020807 mRNA. Translation: AAH20807.1. | |
| IPI | IPI00025019. |
| PIR | SNHUC5. S15973. |
| RefSeq | NP_002784.1. |
| UniGene | Hs.352768 Hs.590872 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1RYP based on UniProtKB P23724. |
| SMR | P20618. Positions 29-241. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P20618. 24 interactions. |
Protein family/group databases | |
| MEROPS | T01.986. |
PTM databases | |
| PhosphoSite | P20618. |
2-D gel databases | |
| REPRODUCTION-2DPAGE | IPI00025019. |
Proteomic databases | |
| PeptideAtlas | P20618. |
| PRIDE | P20618. |
Genome annotation databases | |
| Ensembl | ENSG00000008018. Homo sapiens. [Contig view] |
| GeneID | 5689. |
| KEGG | hsa:5689. |
| UCSC | uc003qxr.1. human. |
Organism-specific databases | |
| GeneCards | GC06M170761. |
| H-InvDB | HIX0006400. |
| HGNC | HGNC:9537. PSMB1. |
| MIM | 602017. gene. |
| PharmGKB | PA33882. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | P20618. |
| HOVERGEN | P20618. |
| OMA | P20618. EREQCRA. |
Enzyme and pathway databases | |
| BRENDA | 3.4.25.1. 247. |
| Reactome | REACT_11045. Signaling by Wnt. REACT_13. Metabolism of amino acids. REACT_13635. Regulation of activated PAK-2p34 by proteasome mediated degradation. REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. REACT_578. Apoptosis. REACT_6185. HIV Infection. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_9035. APC/C:Cdh1-mediated degradation of Skp2. |
Gene expression databases | |
| ArrayExpress | P20618. |
| Bgee | P20618. |
| CleanEx | HS_PSMB1. |
| GermOnline | ENSG00000008018. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001353. Proteasome_alpha_beta_su. IPR016050. Proteasome_bsu_CS. [Graphical view] |
| Pfam | PF00227. Proteasome. 1 hit. [Graphical view] |
| PROSITE | PS00854. PROTEASOME_B. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00188. Bortezomib. |
| NextBio | 22098. |
| SOURCE | Search... |
Entry information
| Entry name | PSB1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P20618 Secondary accession number(s): B5BU76, Q9BWA8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 6 Human chromosome 6: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


