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P20612 (GNAT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine nucleotide-binding protein G(t) subunit alpha-1
Alternative name(s):
Transducin alpha-1 chain
Gene names
Name:Gnat1
Synonyms:Gnat-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.

Subunit structure

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons By similarity.

Tissue specificity

Rod.

Sequence similarities

Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Ontologies

Keywords
   Biological processSensory transduction
Vision
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionTransducer
   PTMLipoprotein
Myristate
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 14712229. Source: MGI

G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger

Traceable author statement Ref.1. Source: MGI

adenylate cyclase-modulating G-protein coupled receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

cell proliferation

Inferred from direct assay PubMed 14712229. Source: MGI

cellular response to electrical stimulus

Inferred from mutant phenotype PubMed 21052544. Source: MGI

detection of chemical stimulus involved in sensory perception of bitter taste

Inferred from Biological aspect of Ancestor. Source: RefGenome

detection of light stimulus involved in visual perception

Inferred from mutant phenotype PubMed 18171936. Source: MGI

eye photoreceptor cell development

Inferred from mutant phenotype PubMed 16249514. Source: MGI

phototransduction

Inferred from mutant phenotype PubMed 11095744PubMed 11853756PubMed 15073279PubMed 16249514PubMed 17881533PubMed 18367617. Source: MGI

positive regulation of cyclic-nucleotide phosphodiesterase activity

Inferred from electronic annotation. Source: Ensembl

response to light intensity

Inferred from electronic annotation. Source: Ensembl

retina development in camera-type eye

Inferred from mutant phenotype PubMed 21052544. Source: MGI

rhodopsin mediated signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

sensory perception of umami taste

Inferred from mutant phenotype PubMed 15342734. Source: MGI

visual perception

Inferred from mutant phenotype PubMed 16603466. Source: MGI

   Cellular_componentapical plasma membrane

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: Ensembl

extrinsic component of cytoplasmic side of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

heterotrimeric G-protein complex

Inferred from sequence alignment Ref.1. Source: MGI

membrane

Traceable author statement PubMed 3923359. Source: MGI

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

photoreceptor connecting cilium

Inferred from direct assay PubMed 17881533. Source: MGI

photoreceptor inner segment

Inferred from Biological aspect of Ancestor. Source: RefGenome

photoreceptor outer segment

Inferred from direct assay PubMed 18367617PubMed 20592197. Source: MGI

   Molecular_functionG-protein beta/gamma-subunit complex binding

Inferred from direct assay PubMed 17881533. Source: MGI

G-protein coupled receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

GTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from direct assay PubMed 17881533. Source: MGI

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 17881533. Source: MGI

signal transducer activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 350349Guanine nucleotide-binding protein G(t) subunit alpha-1
PRO_0000203738

Regions

Nucleotide binding36 – 438GTP By similarity
Nucleotide binding171 – 1777GTP By similarity
Nucleotide binding196 – 2005GTP By similarity
Nucleotide binding265 – 2684GTP By similarity

Sites

Metal binding431Magnesium By similarity
Metal binding1771Magnesium By similarity
Binding site3221GTP; via amide nitrogen By similarity

Amino acid modifications

Modified residue1421Phosphotyrosine By similarity
Lipidation21N-myristoyl glycine By similarity

Sequences

Sequence LengthMass (Da)Tools
P20612 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 3810BA8DEEFBD7D5

FASTA35039,967
        10         20         30         40         50         60 
MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK IIHQDGYSLE 

        70         80         90        100        110        120 
ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA RKLMHMADTI EEGTMPKEMS 

       130        140        150        160        170        180 
DIIQRLWKDS GIQACFDRAS EYQLNDSAGY YLSDLERLVT PGYVPTEQDV LRSRVKTTGI 

       190        200        210        220        230        240 
IETQFSFKDL NFRMFDVGGQ RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH 

       250        260        270        280        290        300 
ESLHLFNSIC NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK 

       310        320        330        340        350 
VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the mouse rod transducin alpha subunit gene."
Raport C.J., Dere B., Hurley J.B.
J. Biol. Chem. 264:7122-7128(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[3]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 32-42; 194-201; 267-273 AND 330-341, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[4]"G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development."
Williams C.J., Schultz R.M., Kopf G.S.
Mol. Reprod. Dev. 44:315-323(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 124-317.
Strain: CF-1 / Harlan.
Tissue: Retina.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M25513 expand/collapse EMBL AC list , M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA. Translation: AAA40473.1.
BC022793 mRNA. Translation: AAH22793.1.
BC051412 mRNA. Translation: AAH51412.2.
BC058810 mRNA. Translation: AAH58810.1.
U38504 mRNA. Translation: AAB01735.1.
CCDSCCDS23504.1.
PIRRGMST1. A33352.
RefSeqNP_032166.1. NM_008140.2.
UniGeneMm.284853.

3D structure databases

ProteinModelPortalP20612.
SMRP20612. Positions 27-344.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000010205.

PTM databases

PhosphoSiteP20612.

Proteomic databases

MaxQBP20612.
PaxDbP20612.
PRIDEP20612.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837.
GeneID14685.
KEGGmmu:14685.
UCSCuc009rmr.1. mouse.

Organism-specific databases

CTD2779.
MGIMGI:95778. Gnat1.

Phylogenomic databases

eggNOGNOG322962.
GeneTreeENSGT00690000102007.
HOGENOMHOG000038730.
HOVERGENHBG063184.
InParanoidP20612.
KOK04631.
OMAMHLADTI.
OrthoDBEOG72C50B.
PhylomeDBP20612.
TreeFamTF300673.

Gene expression databases

BgeeP20612.
GenevestigatorP20612.

Family and domain databases

Gene3D1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10218. PTHR10218. 1 hit.
PfamPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

ChiTaRSGNAT1. mouse.
NextBio286619.
PROP20612.
SOURCESearch...

Entry information

Entry nameGNAT1_MOUSE
AccessionPrimary (citable) accession number: P20612
Secondary accession number(s): Q80X34
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot