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Protein

Guanine nucleotide-binding protein G(t) subunit alpha-1

Gene

Gnat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431MagnesiumBy similarity
Metal bindingi177 – 1771MagnesiumBy similarity
Binding sitei322 – 3221GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 438GTPBy similarity
Nucleotide bindingi171 – 1777GTPBy similarity
Nucleotide bindingi196 – 2005GTPBy similarity
Nucleotide bindingi265 – 2684GTPBy similarity

GO - Molecular functioni

  • G-protein beta/gamma-subunit complex binding Source: MGI
  • G-protein coupled receptor binding Source: GO_Central
  • GTPase activity Source: MGI
  • GTP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • signal transducer activity Source: GO_Central

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: GO_Central
  • cell proliferation Source: MGI
  • cellular response to electrical stimulus Source: MGI
  • detection of chemical stimulus involved in sensory perception of bitter taste Source: Ensembl
  • detection of light stimulus involved in visual perception Source: MGI
  • eye photoreceptor cell development Source: MGI
  • G-protein coupled receptor signaling pathway Source: MGI
  • G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Source: MGI
  • metabolic process Source: GOC
  • phototransduction Source: MGI
  • phototransduction, visible light Source: MGI
  • positive regulation of cyclic-nucleotide phosphodiesterase activity Source: Ensembl
  • response to light intensity Source: Ensembl
  • retina development in camera-type eye Source: MGI
  • rhodopsin mediated signaling pathway Source: Ensembl
  • sensory perception of umami taste Source: MGI
  • visual perception Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_303206. G-protein activation.
REACT_311545. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_319215. PLC beta mediated events.
REACT_326042. Activation of the phototransduction cascade.
REACT_331048. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(t) subunit alpha-1
Alternative name(s):
Transducin alpha-1 chain
Gene namesi
Name:Gnat1
Synonyms:Gnat-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:95778. Gnat1.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: Ensembl
  • cytoplasm Source: Ensembl
  • heterotrimeric G-protein complex Source: MGI
  • membrane Source: MGI
  • neuronal cell body Source: Ensembl
  • photoreceptor connecting cilium Source: MGI
  • photoreceptor inner segment Source: MGI
  • photoreceptor outer segment Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 350349Guanine nucleotide-binding protein G(t) subunit alpha-1PRO_0000203738Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei142 – 1421PhosphotyrosineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQBiP20612.
PaxDbiP20612.
PRIDEiP20612.

PTM databases

PhosphoSiteiP20612.

Expressioni

Tissue specificityi

Rod.

Gene expression databases

BgeeiP20612.
GenevisibleiP20612. MM.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010205.

Structurei

3D structure databases

ProteinModelPortaliP20612.
SMRiP20612. Positions 27-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiNOG322962.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP20612.
KOiK04631.
OMAiMHLADTI.
OrthoDBiEOG72C50B.
PhylomeDBiP20612.
TreeFamiTF300673.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK
60 70 80 90 100
IIHQDGYSLE ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA
110 120 130 140 150
RKLMHMADTI EEGTMPKEMS DIIQRLWKDS GIQACFDRAS EYQLNDSAGY
160 170 180 190 200
YLSDLERLVT PGYVPTEQDV LRSRVKTTGI IETQFSFKDL NFRMFDVGGQ
210 220 230 240 250
RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH ESLHLFNSIC
260 270 280 290 300
NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK
310 320 330 340 350
VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF
Length:350
Mass (Da):39,967
Last modified:January 23, 2007 - v3
Checksum:i3810BA8DEEFBD7D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25513
, M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA. Translation: AAA40473.1.
BC022793 mRNA. Translation: AAH22793.1.
BC051412 mRNA. Translation: AAH51412.2.
BC058810 mRNA. Translation: AAH58810.1.
U38504 mRNA. Translation: AAB01735.1.
CCDSiCCDS23504.1.
PIRiA33352. RGMST1.
RefSeqiNP_032166.1. NM_008140.2.
UniGeneiMm.284853.

Genome annotation databases

EnsembliENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837.
GeneIDi14685.
KEGGimmu:14685.
UCSCiuc009rmr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25513
, M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA. Translation: AAA40473.1.
BC022793 mRNA. Translation: AAH22793.1.
BC051412 mRNA. Translation: AAH51412.2.
BC058810 mRNA. Translation: AAH58810.1.
U38504 mRNA. Translation: AAB01735.1.
CCDSiCCDS23504.1.
PIRiA33352. RGMST1.
RefSeqiNP_032166.1. NM_008140.2.
UniGeneiMm.284853.

3D structure databases

ProteinModelPortaliP20612.
SMRiP20612. Positions 27-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010205.

PTM databases

PhosphoSiteiP20612.

Proteomic databases

MaxQBiP20612.
PaxDbiP20612.
PRIDEiP20612.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837.
GeneIDi14685.
KEGGimmu:14685.
UCSCiuc009rmr.1. mouse.

Organism-specific databases

CTDi2779.
MGIiMGI:95778. Gnat1.

Phylogenomic databases

eggNOGiNOG322962.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP20612.
KOiK04631.
OMAiMHLADTI.
OrthoDBiEOG72C50B.
PhylomeDBiP20612.
TreeFamiTF300673.

Enzyme and pathway databases

ReactomeiREACT_303206. G-protein activation.
REACT_311545. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_319215. PLC beta mediated events.
REACT_326042. Activation of the phototransduction cascade.
REACT_331048. G alpha (i) signalling events.

Miscellaneous databases

ChiTaRSiGnat1. mouse.
NextBioi286619.
PROiP20612.
SOURCEiSearch...

Gene expression databases

BgeeiP20612.
GenevisibleiP20612. MM.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the mouse rod transducin alpha subunit gene."
    Raport C.J., Dere B., Hurley J.B.
    J. Biol. Chem. 264:7122-7128(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 32-42; 194-201; 267-273 AND 330-341, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  4. "G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development."
    Williams C.J., Schultz R.M., Kopf G.S.
    Mol. Reprod. Dev. 44:315-323(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 124-317.
    Strain: CF-1 / Harlan.
    Tissue: Retina.

Entry informationi

Entry nameiGNAT1_MOUSE
AccessioniPrimary (citable) accession number: P20612
Secondary accession number(s): Q80X34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.