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P20612

- GNAT1_MOUSE

UniProt

P20612 - GNAT1_MOUSE

Protein

Guanine nucleotide-binding protein G(t) subunit alpha-1

Gene

Gnat1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi43 – 431MagnesiumBy similarity
    Metal bindingi177 – 1771MagnesiumBy similarity
    Binding sitei322 – 3221GTP; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi36 – 438GTPBy similarity
    Nucleotide bindingi171 – 1777GTPBy similarity
    Nucleotide bindingi196 – 2005GTPBy similarity
    Nucleotide bindingi265 – 2684GTPBy similarity

    GO - Molecular functioni

    1. G-protein beta/gamma-subunit complex binding Source: MGI
    2. G-protein coupled receptor binding Source: RefGenome
    3. GTPase activity Source: MGI
    4. GTP binding Source: UniProtKB-KW
    5. metal ion binding Source: UniProtKB-KW
    6. protein binding Source: MGI
    7. signal transducer activity Source: RefGenome

    GO - Biological processi

    1. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RefGenome
    2. cell proliferation Source: MGI
    3. cellular response to electrical stimulus Source: MGI
    4. detection of chemical stimulus involved in sensory perception of bitter taste Source: Ensembl
    5. detection of light stimulus involved in visual perception Source: MGI
    6. eye photoreceptor cell development Source: MGI
    7. G-protein coupled receptor signaling pathway Source: MGI
    8. G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Source: MGI
    9. phototransduction Source: MGI
    10. positive regulation of cyclic-nucleotide phosphodiesterase activity Source: Ensembl
    11. response to light intensity Source: Ensembl
    12. retina development in camera-type eye Source: MGI
    13. rhodopsin mediated signaling pathway Source: Ensembl
    14. sensory perception of umami taste Source: MGI
    15. visual perception Source: MGI

    Keywords - Molecular functioni

    Transducer

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_188252. Activation of the phototransduction cascade.
    REACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.
    REACT_205726. G-protein activation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein G(t) subunit alpha-1
    Alternative name(s):
    Transducin alpha-1 chain
    Gene namesi
    Name:Gnat1
    Synonyms:Gnat-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:95778. Gnat1.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: Ensembl
    2. cytoplasm Source: Ensembl
    3. heterotrimeric G-protein complex Source: MGI
    4. membrane Source: MGI
    5. neuronal cell body Source: Ensembl
    6. photoreceptor connecting cilium Source: MGI
    7. photoreceptor inner segment Source: Ensembl
    8. photoreceptor outer segment Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 350349Guanine nucleotide-binding protein G(t) subunit alpha-1PRO_0000203738Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Modified residuei142 – 1421PhosphotyrosineBy similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Phosphoprotein

    Proteomic databases

    MaxQBiP20612.
    PaxDbiP20612.
    PRIDEiP20612.

    PTM databases

    PhosphoSiteiP20612.

    Expressioni

    Tissue specificityi

    Rod.

    Gene expression databases

    BgeeiP20612.
    GenevestigatoriP20612.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000010205.

    Structurei

    3D structure databases

    ProteinModelPortaliP20612.
    SMRiP20612. Positions 27-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG322962.
    GeneTreeiENSGT00690000102007.
    HOGENOMiHOG000038730.
    HOVERGENiHBG063184.
    InParanoidiP20612.
    KOiK04631.
    OMAiMHLADTI.
    OrthoDBiEOG72C50B.
    PhylomeDBiP20612.
    TreeFamiTF300673.

    Family and domain databases

    Gene3Di1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR001408. Gprotein_alpha_I.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10218. PTHR10218. 1 hit.
    PfamiPF00503. G-alpha. 1 hit.
    [Graphical view]
    PRINTSiPR00318. GPROTEINA.
    PR00441. GPROTEINAI.
    SMARTiSM00275. G_alpha. 1 hit.
    [Graphical view]
    SUPFAMiSSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P20612-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK    50
    IIHQDGYSLE ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA 100
    RKLMHMADTI EEGTMPKEMS DIIQRLWKDS GIQACFDRAS EYQLNDSAGY 150
    YLSDLERLVT PGYVPTEQDV LRSRVKTTGI IETQFSFKDL NFRMFDVGGQ 200
    RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH ESLHLFNSIC 250
    NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK 300
    VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF 350
    Length:350
    Mass (Da):39,967
    Last modified:January 23, 2007 - v3
    Checksum:i3810BA8DEEFBD7D5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M25513
    , M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA. Translation: AAA40473.1.
    BC022793 mRNA. Translation: AAH22793.1.
    BC051412 mRNA. Translation: AAH51412.2.
    BC058810 mRNA. Translation: AAH58810.1.
    U38504 mRNA. Translation: AAB01735.1.
    CCDSiCCDS23504.1.
    PIRiA33352. RGMST1.
    RefSeqiNP_032166.1. NM_008140.2.
    UniGeneiMm.284853.

    Genome annotation databases

    EnsembliENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837.
    GeneIDi14685.
    KEGGimmu:14685.
    UCSCiuc009rmr.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M25513
    , M25506 , M25507 , M25508 , M25509 , M25510 , M25511 , M25512 Genomic DNA. Translation: AAA40473.1 .
    BC022793 mRNA. Translation: AAH22793.1 .
    BC051412 mRNA. Translation: AAH51412.2 .
    BC058810 mRNA. Translation: AAH58810.1 .
    U38504 mRNA. Translation: AAB01735.1 .
    CCDSi CCDS23504.1.
    PIRi A33352. RGMST1.
    RefSeqi NP_032166.1. NM_008140.2.
    UniGenei Mm.284853.

    3D structure databases

    ProteinModelPortali P20612.
    SMRi P20612. Positions 27-344.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000010205.

    PTM databases

    PhosphoSitei P20612.

    Proteomic databases

    MaxQBi P20612.
    PaxDbi P20612.
    PRIDEi P20612.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000010205 ; ENSMUSP00000010205 ; ENSMUSG00000034837 .
    GeneIDi 14685.
    KEGGi mmu:14685.
    UCSCi uc009rmr.1. mouse.

    Organism-specific databases

    CTDi 2779.
    MGIi MGI:95778. Gnat1.

    Phylogenomic databases

    eggNOGi NOG322962.
    GeneTreei ENSGT00690000102007.
    HOGENOMi HOG000038730.
    HOVERGENi HBG063184.
    InParanoidi P20612.
    KOi K04631.
    OMAi MHLADTI.
    OrthoDBi EOG72C50B.
    PhylomeDBi P20612.
    TreeFami TF300673.

    Enzyme and pathway databases

    Reactomei REACT_188252. Activation of the phototransduction cascade.
    REACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.
    REACT_205726. G-protein activation.

    Miscellaneous databases

    ChiTaRSi GNAT1. mouse.
    NextBioi 286619.
    PROi P20612.
    SOURCEi Search...

    Gene expression databases

    Bgeei P20612.
    Genevestigatori P20612.

    Family and domain databases

    Gene3Di 1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR001408. Gprotein_alpha_I.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10218. PTHR10218. 1 hit.
    Pfami PF00503. G-alpha. 1 hit.
    [Graphical view ]
    PRINTSi PR00318. GPROTEINA.
    PR00441. GPROTEINAI.
    SMARTi SM00275. G_alpha. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the mouse rod transducin alpha subunit gene."
      Raport C.J., Dere B., Hurley J.B.
      J. Biol. Chem. 264:7122-7128(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    3. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 32-42; 194-201; 267-273 AND 330-341, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    4. "G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development."
      Williams C.J., Schultz R.M., Kopf G.S.
      Mol. Reprod. Dev. 44:315-323(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 124-317.
      Strain: CF-1 / Harlan.
      Tissue: Retina.

    Entry informationi

    Entry nameiGNAT1_MOUSE
    AccessioniPrimary (citable) accession number: P20612
    Secondary accession number(s): Q80X34
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 140 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3