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Protein

Guanine nucleotide-binding protein G(t) subunit alpha-1

Gene

Gnat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43MagnesiumBy similarity1
Metal bindingi177MagnesiumBy similarity1
Binding sitei322GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43GTPBy similarity8
Nucleotide bindingi171 – 177GTPBy similarity7
Nucleotide bindingi196 – 200GTPBy similarity5
Nucleotide bindingi265 – 268GTPBy similarity4

GO - Molecular functioni

  • G-protein beta/gamma-subunit complex binding Source: MGI
  • G-protein coupled receptor binding Source: GO_Central
  • GTPase activity Source: MGI
  • GTP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • signal transducer activity Source: GO_Central

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: GO_Central
  • cell proliferation Source: MGI
  • cellular response to electrical stimulus Source: MGI
  • detection of chemical stimulus involved in sensory perception of bitter taste Source: Ensembl
  • detection of light stimulus involved in visual perception Source: MGI
  • eye photoreceptor cell development Source: MGI
  • G-protein coupled receptor signaling pathway Source: MGI
  • G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Source: MGI
  • phototransduction Source: MGI
  • phototransduction, visible light Source: MGI
  • positive regulation of cyclic-nucleotide phosphodiesterase activity Source: Ensembl
  • response to light intensity Source: Ensembl
  • retina development in camera-type eye Source: MGI
  • rhodopsin mediated signaling pathway Source: Ensembl
  • sensory perception of umami taste Source: MGI
  • visual perception Source: MGI

Keywordsi

Molecular functionTransducer
Biological processSensory transduction, Vision
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-112043. PLC beta mediated events.
R-MMU-202040. G-protein activation.
R-MMU-2485179. Activation of the phototransduction cascade.
R-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-MMU-418594. G alpha (i) signalling events.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(t) subunit alpha-1
Alternative name(s):
Transducin alpha-1 chain
Gene namesi
Name:Gnat1
Synonyms:Gnat-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:95778. Gnat1.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: Ensembl
  • cytoplasm Source: Ensembl
  • heterotrimeric G-protein complex Source: MGI
  • membrane Source: MGI
  • neuronal cell body Source: Ensembl
  • photoreceptor connecting cilium Source: MGI
  • photoreceptor inner segment Source: MGI
  • photoreceptor outer segment Source: MGI

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002037382 – 350Guanine nucleotide-binding protein G(t) subunit alpha-1Add BLAST349

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei142PhosphotyrosineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQBiP20612.
PaxDbiP20612.
PRIDEiP20612.

PTM databases

iPTMnetiP20612.
PhosphoSitePlusiP20612.

Expressioni

Tissue specificityi

Rod.

Gene expression databases

BgeeiENSMUSG00000034837.
ExpressionAtlasiP20612. baseline and differential.
GenevisibleiP20612. MM.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010205.

Structurei

3D structure databases

ProteinModelPortaliP20612.
SMRiP20612.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP20612.
KOiK04631.
OMAiECLEFIV.
OrthoDBiEOG091G0VUT.
PhylomeDBiP20612.
TreeFamiTF300673.

Family and domain databases

CDDicd00066. G-alpha. 1 hit.
Gene3Di1.10.400.10. 1 hit.
InterProiView protein in InterPro
IPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiView protein in Pfam
PF00503. G-alpha. 1 hit.
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiView protein in SMART
SM00275. G_alpha. 1 hit.
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK
60 70 80 90 100
IIHQDGYSLE ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA
110 120 130 140 150
RKLMHMADTI EEGTMPKEMS DIIQRLWKDS GIQACFDRAS EYQLNDSAGY
160 170 180 190 200
YLSDLERLVT PGYVPTEQDV LRSRVKTTGI IETQFSFKDL NFRMFDVGGQ
210 220 230 240 250
RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH ESLHLFNSIC
260 270 280 290 300
NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK
310 320 330 340 350
VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF
Length:350
Mass (Da):39,967
Last modified:January 23, 2007 - v3
Checksum:i3810BA8DEEFBD7D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25513
, M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA. Translation: AAA40473.1.
BC022793 mRNA. Translation: AAH22793.1.
BC051412 mRNA. Translation: AAH51412.2.
BC058810 mRNA. Translation: AAH58810.1.
U38504 mRNA. Translation: AAB01735.1.
CCDSiCCDS23504.1.
PIRiA33352. RGMST1.
RefSeqiNP_032166.1. NM_008140.2.
UniGeneiMm.284853.

Genome annotation databases

EnsembliENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837.
GeneIDi14685.
KEGGimmu:14685.
UCSCiuc009rmr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25513
, M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA. Translation: AAA40473.1.
BC022793 mRNA. Translation: AAH22793.1.
BC051412 mRNA. Translation: AAH51412.2.
BC058810 mRNA. Translation: AAH58810.1.
U38504 mRNA. Translation: AAB01735.1.
CCDSiCCDS23504.1.
PIRiA33352. RGMST1.
RefSeqiNP_032166.1. NM_008140.2.
UniGeneiMm.284853.

3D structure databases

ProteinModelPortaliP20612.
SMRiP20612.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010205.

PTM databases

iPTMnetiP20612.
PhosphoSitePlusiP20612.

Proteomic databases

MaxQBiP20612.
PaxDbiP20612.
PRIDEiP20612.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837.
GeneIDi14685.
KEGGimmu:14685.
UCSCiuc009rmr.1. mouse.

Organism-specific databases

CTDi2779.
MGIiMGI:95778. Gnat1.

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP20612.
KOiK04631.
OMAiECLEFIV.
OrthoDBiEOG091G0VUT.
PhylomeDBiP20612.
TreeFamiTF300673.

Enzyme and pathway databases

ReactomeiR-MMU-112043. PLC beta mediated events.
R-MMU-202040. G-protein activation.
R-MMU-2485179. Activation of the phototransduction cascade.
R-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-MMU-418594. G alpha (i) signalling events.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Miscellaneous databases

ChiTaRSiGnat1. mouse.
PROiPR:P20612.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034837.
ExpressionAtlasiP20612. baseline and differential.
GenevisibleiP20612. MM.

Family and domain databases

CDDicd00066. G-alpha. 1 hit.
Gene3Di1.10.400.10. 1 hit.
InterProiView protein in InterPro
IPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiView protein in Pfam
PF00503. G-alpha. 1 hit.
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiView protein in SMART
SM00275. G_alpha. 1 hit.
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGNAT1_MOUSE
AccessioniPrimary (citable) accession number: P20612
Secondary accession number(s): Q80X34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: May 10, 2017
This is version 163 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.