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P20612

- GNAT1_MOUSE

UniProt

P20612 - GNAT1_MOUSE

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Protein

Guanine nucleotide-binding protein G(t) subunit alpha-1

Gene

Gnat1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431MagnesiumBy similarity
Metal bindingi177 – 1771MagnesiumBy similarity
Binding sitei322 – 3221GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 438GTPBy similarity
Nucleotide bindingi171 – 1777GTPBy similarity
Nucleotide bindingi196 – 2005GTPBy similarity
Nucleotide bindingi265 – 2684GTPBy similarity

GO - Molecular functioni

  1. G-protein beta/gamma-subunit complex binding Source: MGI
  2. G-protein coupled receptor binding Source: RefGenome
  3. GTPase activity Source: MGI
  4. GTP binding Source: UniProtKB-KW
  5. metal ion binding Source: UniProtKB-KW
  6. signal transducer activity Source: RefGenome

GO - Biological processi

  1. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RefGenome
  2. cell proliferation Source: MGI
  3. cellular response to electrical stimulus Source: MGI
  4. detection of chemical stimulus involved in sensory perception of bitter taste Source: Ensembl
  5. detection of light stimulus involved in visual perception Source: MGI
  6. eye photoreceptor cell development Source: MGI
  7. G-protein coupled receptor signaling pathway Source: MGI
  8. G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Source: MGI
  9. phototransduction Source: MGI
  10. positive regulation of cyclic-nucleotide phosphodiesterase activity Source: Ensembl
  11. response to light intensity Source: Ensembl
  12. retina development in camera-type eye Source: MGI
  13. rhodopsin mediated signaling pathway Source: Ensembl
  14. sensory perception of umami taste Source: MGI
  15. visual perception Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_188252. Activation of the phototransduction cascade.
REACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_205726. G-protein activation.
REACT_250376. G alpha (i) signalling events.
REACT_253342. PLC beta mediated events.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(t) subunit alpha-1
Alternative name(s):
Transducin alpha-1 chain
Gene namesi
Name:Gnat1
Synonyms:Gnat-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:95778. Gnat1.

Subcellular locationi

GO - Cellular componenti

  1. apical plasma membrane Source: Ensembl
  2. cytoplasm Source: Ensembl
  3. heterotrimeric G-protein complex Source: MGI
  4. membrane Source: MGI
  5. neuronal cell body Source: Ensembl
  6. photoreceptor connecting cilium Source: MGI
  7. photoreceptor inner segment Source: Ensembl
  8. photoreceptor outer segment Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 350349Guanine nucleotide-binding protein G(t) subunit alpha-1PRO_0000203738Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei142 – 1421PhosphotyrosineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQBiP20612.
PaxDbiP20612.
PRIDEiP20612.

PTM databases

PhosphoSiteiP20612.

Expressioni

Tissue specificityi

Rod.

Gene expression databases

BgeeiP20612.
GenevestigatoriP20612.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010205.

Structurei

3D structure databases

ProteinModelPortaliP20612.
SMRiP20612. Positions 27-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiNOG322962.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP20612.
KOiK04631.
OMAiMHLADTI.
OrthoDBiEOG72C50B.
PhylomeDBiP20612.
TreeFamiTF300673.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20612-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK
60 70 80 90 100
IIHQDGYSLE ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA
110 120 130 140 150
RKLMHMADTI EEGTMPKEMS DIIQRLWKDS GIQACFDRAS EYQLNDSAGY
160 170 180 190 200
YLSDLERLVT PGYVPTEQDV LRSRVKTTGI IETQFSFKDL NFRMFDVGGQ
210 220 230 240 250
RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH ESLHLFNSIC
260 270 280 290 300
NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK
310 320 330 340 350
VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF
Length:350
Mass (Da):39,967
Last modified:January 23, 2007 - v3
Checksum:i3810BA8DEEFBD7D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25513
, M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA. Translation: AAA40473.1.
BC022793 mRNA. Translation: AAH22793.1.
BC051412 mRNA. Translation: AAH51412.2.
BC058810 mRNA. Translation: AAH58810.1.
U38504 mRNA. Translation: AAB01735.1.
CCDSiCCDS23504.1.
PIRiA33352. RGMST1.
RefSeqiNP_032166.1. NM_008140.2.
UniGeneiMm.284853.

Genome annotation databases

EnsembliENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837.
GeneIDi14685.
KEGGimmu:14685.
UCSCiuc009rmr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25513
, M25506 , M25507 , M25508 , M25509 , M25510 , M25511 , M25512 Genomic DNA. Translation: AAA40473.1 .
BC022793 mRNA. Translation: AAH22793.1 .
BC051412 mRNA. Translation: AAH51412.2 .
BC058810 mRNA. Translation: AAH58810.1 .
U38504 mRNA. Translation: AAB01735.1 .
CCDSi CCDS23504.1.
PIRi A33352. RGMST1.
RefSeqi NP_032166.1. NM_008140.2.
UniGenei Mm.284853.

3D structure databases

ProteinModelPortali P20612.
SMRi P20612. Positions 27-344.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000010205.

PTM databases

PhosphoSitei P20612.

Proteomic databases

MaxQBi P20612.
PaxDbi P20612.
PRIDEi P20612.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000010205 ; ENSMUSP00000010205 ; ENSMUSG00000034837 .
GeneIDi 14685.
KEGGi mmu:14685.
UCSCi uc009rmr.1. mouse.

Organism-specific databases

CTDi 2779.
MGIi MGI:95778. Gnat1.

Phylogenomic databases

eggNOGi NOG322962.
GeneTreei ENSGT00760000118851.
HOGENOMi HOG000038730.
HOVERGENi HBG063184.
InParanoidi P20612.
KOi K04631.
OMAi MHLADTI.
OrthoDBi EOG72C50B.
PhylomeDBi P20612.
TreeFami TF300673.

Enzyme and pathway databases

Reactomei REACT_188252. Activation of the phototransduction cascade.
REACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_205726. G-protein activation.
REACT_250376. G alpha (i) signalling events.
REACT_253342. PLC beta mediated events.

Miscellaneous databases

ChiTaRSi Gnat1. mouse.
NextBioi 286619.
PROi P20612.
SOURCEi Search...

Gene expression databases

Bgeei P20612.
Genevestigatori P20612.

Family and domain databases

Gene3Di 1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10218. PTHR10218. 1 hit.
Pfami PF00503. G-alpha. 1 hit.
[Graphical view ]
PRINTSi PR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTi SM00275. G_alpha. 1 hit.
[Graphical view ]
SUPFAMi SSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the mouse rod transducin alpha subunit gene."
    Raport C.J., Dere B., Hurley J.B.
    J. Biol. Chem. 264:7122-7128(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 32-42; 194-201; 267-273 AND 330-341, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  4. "G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development."
    Williams C.J., Schultz R.M., Kopf G.S.
    Mol. Reprod. Dev. 44:315-323(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 124-317.
    Strain: CF-1 / Harlan.
    Tissue: Retina.

Entry informationi

Entry nameiGNAT1_MOUSE
AccessioniPrimary (citable) accession number: P20612
Secondary accession number(s): Q80X34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3