P20612 (GNAT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein G(t) subunit alpha-1

Alternative name(s):
Transducin alpha-1 chain

Gene names

Name:	Gnat1
Synonyms:	Gnat-1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
Subunit structure	G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons By similarity.
Tissue specificity	Rod.
Sequence similarities	Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transducer
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Traceable author statement Ref.1. Source: MGI adenylate cyclase-modulating G-protein coupled receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome cell proliferation Inferred from direct assay PubMed 14712229. Source: MGI cellular response to electrical stimulus Inferred from mutant phenotype PubMed 21052544. Source: MGI detection of chemical stimulus involved in sensory perception of bitter taste Inferred from Biological aspect of Ancestor. Source: RefGenome detection of light stimulus involved in visual perception Inferred from mutant phenotype PubMed 18171936. Source: MGI eye photoreceptor cell development Inferred from mutant phenotype PubMed 16249514. Source: MGI positive regulation of cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: Compara response to light intensity Inferred from electronic annotation. Source: Compara retina development in camera-type eye Inferred from mutant phenotype PubMed 21052544. Source: MGI rhodopsin mediated signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome sensory perception of umami taste Inferred from mutant phenotype PubMed 15342734. Source: MGI
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: Compara heterotrimeric G-protein complex Inferred from sequence alignment Ref.1. Source: MGI neuronal cell body Inferred from electronic annotation. Source: Compara photoreceptor connecting cilium Inferred from direct assay PubMed 17881533. Source: MGI photoreceptor inner segment Inferred from Biological aspect of Ancestor. Source: RefGenome photoreceptor outer segment Inferred from direct assay PubMed 18367617. Source: MGI
Molecular_function	G-protein beta/gamma-subunit complex binding Inferred from direct assay PubMed 17881533. Source: MGI G-protein coupled receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay PubMed 17881533. Source: MGI metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 350	349	Guanine nucleotide-binding protein G(t) subunit alpha-1		PRO_0000203738
Regions
	Nucleotide binding	36 – 43	8	GTP By similarity
	Nucleotide binding	171 – 177	7	GTP By similarity
	Nucleotide binding	196 – 200	5	GTP By similarity
	Nucleotide binding	265 – 268	4	GTP By similarity
Sites
	Metal binding	43	1	Magnesium By similarity
	Metal binding	177	1	Magnesium By similarity
	Binding site	322	1	GTP; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	142	1	Phosphotyrosine By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20612 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3810BA8DEEFBD7D5	FASTA	350	39,967
	10 20 30 40 50 60 MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK IIHQDGYSLE 70 80 90 100 110 120 ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA RKLMHMADTI EEGTMPKEMS 130 140 150 160 170 180 DIIQRLWKDS GIQACFDRAS EYQLNDSAGY YLSDLERLVT PGYVPTEQDV LRSRVKTTGI 190 200 210 220 230 240 IETQFSFKDL NFRMFDVGGQ RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH 250 260 270 280 290 300 ESLHLFNSIC NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK 310 320 330 340 350 VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the mouse rod transducin alpha subunit gene." Raport C.J., Dere B., Hurley J.B. J. Biol. Chem. 264:7122-7128(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 32-42; 194-201; 267-273 AND 330-341, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[4]	"G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development." Williams C.J., Schultz R.M., Kopf G.S. Mol. Reprod. Dev. 44:315-323(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 124-317. Strain: CF-1 / Harlan. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M25513 , M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA. Translation: AAA40473.1. BC022793 mRNA. Translation: AAH22793.1. BC051412 mRNA. Translation: AAH51412.2. BC058810 mRNA. Translation: AAH58810.1. U38504 mRNA. Translation: AAB01735.1.
IPI	IPI00330646.
PIR	RGMST1. A33352.
RefSeq	NP_032166.1. NM_008140.2.
UniGene	Mm.284853.
3D structure databases
ProteinModelPortal	P20612.
SMR	P20612. Positions 27-344.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000010205.
PTM databases
PhosphoSite	P20612.
Proteomic databases
PaxDb	P20612.
PRIDE	P20612.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837.
GeneID	14685.
KEGG	mmu:14685.
UCSC	uc009rmr.1. mouse.
Organism-specific databases
CTD	2779.
MGI	MGI:95778. Gnat1.
Phylogenomic databases
eggNOG	NOG322962.
GeneTree	ENSGT00690000102007.
HOGENOM	HOG000038730.
HOVERGEN	HBG063184.
InParanoid	P20612.
KO	K04631.
OMA	MHLADTI.
OrthoDB	EOG47D9GD.
Gene expression databases
Bgee	P20612.
Genevestigator	P20612.
GermOnline	ENSMUSG00000034837. Mus musculus.
Family and domain databases
Gene3D	1.10.400.10. 1 hit.
InterPro	IPR001408. Gprotein_alpha_I. IPR001019. Gprotein_alpha_su. IPR011025. GproteinA_insert. [Graphical view]
PANTHER	PTHR10218. PTHR10218. 1 hit.
Pfam	PF00503. G-alpha. 1 hit. [Graphical view]
PRINTS	PR00318. GPROTEINA. PR00441. GPROTEINAI.
SMART	SM00275. G_alpha. 1 hit. [Graphical view]
SUPFAM	SSF47895. Transducn_insert. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	GNAT1. mouse.
NextBio	286619.
SOURCE	Search...

Entry information

Entry name

GNAT1_MOUSE

Accession

Primary (citable) accession number: P20612
Secondary accession number(s): Q80X34

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 128 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program