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Protein

Guanine nucleotide-binding protein G(t) subunit alpha-1

Gene

Gnat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina (By similarity). Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43MagnesiumBy similarity1
Binding sitei146GTPBy similarity1
Metal bindingi177MagnesiumBy similarity1
Binding sitei199GTP; via amide nitrogenBy similarity1
Binding sitei322GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43GTPBy similarity8
Nucleotide bindingi171 – 177GTPBy similarity7
Nucleotide bindingi265 – 268GTPBy similarity4

GO - Molecular functioni

  • G-protein beta/gamma-subunit complex binding Source: MGI
  • G-protein coupled receptor binding Source: GO_Central
  • GTPase activity Source: MGI
  • GTP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • signal transducer activity Source: GO_Central

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: GO_Central
  • cell proliferation Source: MGI
  • cellular response to electrical stimulus Source: MGI
  • detection of chemical stimulus involved in sensory perception of bitter taste Source: Ensembl
  • detection of light stimulus involved in visual perception Source: MGI
  • eye photoreceptor cell development Source: MGI
  • G-protein coupled receptor signaling pathway Source: MGI
  • G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Source: MGI
  • phototransduction Source: MGI
  • phototransduction, visible light Source: MGI
  • positive regulation of cyclic-nucleotide phosphodiesterase activity Source: Ensembl
  • response to light intensity Source: Ensembl
  • retina development in camera-type eye Source: MGI
  • rhodopsin mediated signaling pathway Source: Ensembl
  • sensory perception of umami taste Source: MGI
  • visual perception Source: MGI

Keywordsi

Molecular functionTransducer
Biological processSensory transduction, Vision
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-112043 PLC beta mediated events
R-MMU-202040 G-protein activation
R-MMU-2485179 Activation of the phototransduction cascade
R-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-MMU-418594 G alpha (i) signalling events
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(t) subunit alpha-1
Alternative name(s):
Transducin alpha-1 chain
Gene namesi
Name:Gnat1
Synonyms:Gnat-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:95778 Gnat1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002037382 – 350Guanine nucleotide-binding protein G(t) subunit alpha-1Add BLAST349

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei142PhosphotyrosineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQBiP20612
PaxDbiP20612
PRIDEiP20612

PTM databases

iPTMnetiP20612
PhosphoSitePlusiP20612

Expressioni

Tissue specificityi

Rod.

Gene expression databases

BgeeiENSMUSG00000034837
ExpressionAtlasiP20612 baseline and differential
GenevisibleiP20612 MM

Interactioni

Subunit structurei

Heterotrimeric G proteins are composed of 3 subunits alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RHO. Interacts with RGS9 and PDE6G (By similarity). Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010205

Structurei

3D structure databases

ProteinModelPortaliP20612
SMRiP20612
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni340 – 350Interaction with RHOBy similarityAdd BLAST11

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082 Eukaryota
ENOG410XNVQ LUCA
GeneTreeiENSGT00760000118851
HOGENOMiHOG000038730
HOVERGENiHBG063184
InParanoidiP20612
KOiK04631
OMAiMHLADTI
OrthoDBiEOG091G0VUT
PhylomeDBiP20612
TreeFamiTF300673

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR001408 Gprotein_alpha_I
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00441 GPROTEINAI
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK
60 70 80 90 100
IIHQDGYSLE ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA
110 120 130 140 150
RKLMHMADTI EEGTMPKEMS DIIQRLWKDS GIQACFDRAS EYQLNDSAGY
160 170 180 190 200
YLSDLERLVT PGYVPTEQDV LRSRVKTTGI IETQFSFKDL NFRMFDVGGQ
210 220 230 240 250
RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH ESLHLFNSIC
260 270 280 290 300
NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK
310 320 330 340 350
VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF
Length:350
Mass (Da):39,967
Last modified:January 23, 2007 - v3
Checksum:i3810BA8DEEFBD7D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25513
, M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA Translation: AAA40473.1
BC022793 mRNA Translation: AAH22793.1
BC051412 mRNA Translation: AAH51412.2
BC058810 mRNA Translation: AAH58810.1
U38504 mRNA Translation: AAB01735.1
CCDSiCCDS23504.1
PIRiA33352 RGMST1
RefSeqiNP_032166.1, NM_008140.2
UniGeneiMm.284853

Genome annotation databases

EnsembliENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837
GeneIDi14685
KEGGimmu:14685
UCSCiuc009rmr.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGNAT1_MOUSE
AccessioniPrimary (citable) accession number: P20612
Secondary accession number(s): Q80X34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: February 28, 2018
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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