Reviewed, UniProtKB/Swiss-Prot P20611 (PPAL_RAT)

Names and origin

Protein names

Recommended name:
    Lysosomal acid phosphatase
      Short name=LAP
    EC=3.1.3.2

Gene names

Name:

Acp2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Subcellular location	Lysosome membrane; Single-pass membrane protein; Lumenal side By similarity. Lysosome lumen By similarity. Note: The soluble form arises by proteolytic processing of the membrane-bound form By similarity.
Post-translational modification	The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen By similarity.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Lysosome Membrane
Domain	Signal Transmembrane
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	response to organic substance Inferred from direct assay. Source: RGD synaptic transmission Non-traceable author statement. Source: RGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal lumen Inferred from electronic annotation. Source: UniProtKB-SubCell synaptosome Inferred from direct assay. Source: RGD
Molecular function	acid phosphatase activity Inferred from direct assay. Source: RGD phosphoprotein phosphatase activity Inferred from direct assay. Source: RGD phosphotyrosine binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30
	Chain	31 – 423	393	Lysosomal acid phosphatase		PRO_0000023962
Regions
	Topological domain	31 – 380	350	Lumenal Potential
	Transmembrane	381 – 401	21	Potential
	Topological domain	402 – 423	22	Cytoplasmic Potential
Sites
	Active site	42	1	Nucleophile By similarity
	Active site	287	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	177	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
	Disulfide bond	159 ↔ 370		By similarity
	Disulfide bond	212 ↔ 310		By similarity
	Disulfide bond	345 ↔ 349		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20611-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 6F4C4819CADD4496	FASTA	423	48,320
	10 20 30 40 50 60 MAGRQSGWSQ AALLQFLLGM CLMVMPPIQA RSLRFVTLLY RHGDRSPVKA YPKDPYQEEK 70 80 90 100 110 120 WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNASYHRQEV YVRSTDFDRT LMSAEANLAG 130 140 150 160 170 180 LFPPTEVQHF NPNISWQPIP VHTVPITEDR LLKFPLGPCP RYEQLQNETR QTPEYQNMSI 190 200 210 220 230 240 QNAQFLDMVA NETGLMNLTL ETIWNVYDTL FCEQTHGLLL PPWASPQTVQ ALSQLKDFSF 250 260 270 280 290 300 LFLFGIHDQV QKARLQGGVL LAQILKNLTL MATTSQFPKL LVYSAHDTTL VALQMALNVY 310 320 330 340 350 360 NGKQAPYASC HIFELYQEDN GNFSVEMYFR NDSKKAPWPL TLPGCPHRCP LQDFLRLTEP 370 380 390 400 410 420 VIPKDWQKEC QLASDTADTE VIVALAVCGS ILFLLIVLLL TVLFRMQAQP PGYHHVADRE DHA

References

[1]	"Isolation and sequencing of a cDNA clone encoding acid phosphatase in rat liver lysosomes." Himeno M., Fujita H., Noguchi Y., Kono A., Kato K. Biochem. Biophys. Res. Commun. 162:1044-1053(1989) [PubMed: 2764916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 35-41; 154-161; 255-266 AND 349-364, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27893 mRNA. Translation: AAA40744.1.
IPI	IPI00201276.
PIR	A33395.
RefSeq	NP_058684.1.
UniGene	Rn.9816
3D structure databases
SMR	P20611. Positions 31-371.
ModBase	Search...
Protein-protein interaction databases
STRING	P20611.
Proteomic databases
PRIDE	P20611.
Genome annotation databases
Ensembl	ENSRNOT00000018620; ENSRNOP00000018620; ENSRNOG00000013594; Rattus norvegicus. [Genome view]
GeneID	24162.
KEGG	rno:24162.
UCSC	NM_016988. rat.
Organism-specific databases
CTD	24162.
RGD	2021. Acp2.
Phylogenomic databases
eggNOG	roNOG12648.
HOVERGEN	P20611.
InParanoid	P20611.
Enzyme and pathway databases
BRENDA	3.1.3.2. 248.
Gene expression databases
ArrayExpress	P20611.
Genevestigator	P20611.
GermOnline	ENSRNOG00000013594. Rattus norvegicus.
Family and domain databases
InterPro	IPR000560. Histidine_acid_Pase. [Graphical view]
Pfam	PF00328. Acid_phosphat_A. 1 hit. [Graphical view]
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	602461.

Entry information

Entry name

PPAL_RAT

Accession

Primary (citable) accession number: P20611

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	February 9, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)