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Protein

Lysosomal acid phosphatase

Gene

Acp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei42NucleophileBy similarity1
Active sitei287Proton donorBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: RGD
  • phosphoprotein phosphatase activity Source: RGD
  • phosphotyrosine residue binding Source: RGD

GO - Biological processi

  • autophagic cell death Source: RGD
  • chemical synaptic transmission Source: RGD
  • lysosome organization Source: RGD
  • response to organic substance Source: RGD
  • skeletal system development Source: RGD

Keywordsi

Molecular functionHydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal acid phosphatase (EC:3.1.3.2)
Short name:
LAP
Gene namesi
Name:Acp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2021. Acp2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini31 – 380LumenalSequence analysisAdd BLAST350
Transmembranei381 – 401HelicalSequence analysisAdd BLAST21
Topological domaini402 – 423CytoplasmicSequence analysisAdd BLAST22

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Add BLAST30
ChainiPRO_000002396231 – 423Lysosomal acid phosphataseAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi92N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi133N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi159 ↔ 370By similarity
Glycosylationi167N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi177N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi191N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi197N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi212 ↔ 310By similarity
Glycosylationi267N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi322N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi331N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi345 ↔ 349By similarity

Post-translational modificationi

The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP20611.
PRIDEiP20611.

Interactioni

GO - Molecular functioni

  • phosphotyrosine residue binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018620.

Structurei

3D structure databases

ProteinModelPortaliP20611.
SMRiP20611.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3720. Eukaryota.
ENOG410ZVBQ. LUCA.
HOGENOMiHOG000231439.
HOVERGENiHBG002203.
InParanoidiP20611.
PhylomeDBiP20611.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiView protein in InterPro
IPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
PfamiView protein in Pfam
PF00328. His_Phos_2. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiView protein in PROSITE
PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRQSGWSQ AALLQFLLGM CLMVMPPIQA RSLRFVTLLY RHGDRSPVKA
60 70 80 90 100
YPKDPYQEEK WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNASYHRQEV
110 120 130 140 150
YVRSTDFDRT LMSAEANLAG LFPPTEVQHF NPNISWQPIP VHTVPITEDR
160 170 180 190 200
LLKFPLGPCP RYEQLQNETR QTPEYQNMSI QNAQFLDMVA NETGLMNLTL
210 220 230 240 250
ETIWNVYDTL FCEQTHGLLL PPWASPQTVQ ALSQLKDFSF LFLFGIHDQV
260 270 280 290 300
QKARLQGGVL LAQILKNLTL MATTSQFPKL LVYSAHDTTL VALQMALNVY
310 320 330 340 350
NGKQAPYASC HIFELYQEDN GNFSVEMYFR NDSKKAPWPL TLPGCPHRCP
360 370 380 390 400
LQDFLRLTEP VIPKDWQKEC QLASDTADTE VIVALAVCGS ILFLLIVLLL
410 420
TVLFRMQAQP PGYHHVADRE DHA
Length:423
Mass (Da):48,320
Last modified:February 1, 1991 - v1
Checksum:i6F4C4819CADD4496
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27893 mRNA. Translation: AAA40744.1.
PIRiA33395.
UniGeneiRn.9816.

Genome annotation databases

UCSCiRGD:2021. rat.

Similar proteinsi

Entry informationi

Entry nameiPPAL_RAT
AccessioniPrimary (citable) accession number: P20611
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 22, 2017
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families