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P20611

- PPAL_RAT

UniProt

P20611 - PPAL_RAT

Protein

Lysosomal acid phosphatase

Gene

Acp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei42 – 421NucleophileBy similarity
    Active sitei287 – 2871Proton donorBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: RGD
    2. phosphoprotein phosphatase activity Source: RGD
    3. phosphotyrosine binding Source: RGD

    GO - Biological processi

    1. autophagic cell death Source: RGD
    2. dephosphorylation Source: GOC
    3. response to organic substance Source: RGD
    4. synaptic transmission Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysosomal acid phosphatase (EC:3.1.3.2)
    Short name:
    LAP
    Gene namesi
    Name:Acp2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2021. Acp2.

    Subcellular locationi

    Lysosome membrane By similarity; Single-pass membrane protein By similarity; Lumenal side By similarity. Lysosome lumen By similarity
    Note: The soluble form arises by proteolytic processing of the membrane-bound form.By similarity

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: RGD
    2. integral component of membrane Source: UniProtKB-KW
    3. lysosomal lumen Source: UniProtKB-SubCell
    4. lysosomal membrane Source: UniProtKB-SubCell
    5. lysosome Source: RGD
    6. neuron projection Source: RGD

    Keywords - Cellular componenti

    Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Add
    BLAST
    Chaini31 – 423393Lysosomal acid phosphatasePRO_0000023962Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi159 ↔ 370By similarity
    Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi212 ↔ 310By similarity
    Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi345 ↔ 349By similarity

    Post-translational modificationi

    The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP20611.
    PRIDEiP20611.

    Expressioni

    Gene expression databases

    GenevestigatoriP20611.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000018620.

    Structurei

    3D structure databases

    ProteinModelPortaliP20611.
    SMRiP20611. Positions 31-371.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini31 – 380350LumenalSequence AnalysisAdd
    BLAST
    Topological domaini402 – 42322CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei381 – 40121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the histidine acid phosphatase family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG85977.
    HOGENOMiHOG000231439.
    HOVERGENiHBG002203.
    InParanoidiP20611.
    PhylomeDBiP20611.

    Family and domain databases

    Gene3Di3.40.50.1240. 1 hit.
    InterProiIPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    [Graphical view]
    PfamiPF00328. His_Phos_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53254. SSF53254. 1 hit.
    PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P20611-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGRQSGWSQ AALLQFLLGM CLMVMPPIQA RSLRFVTLLY RHGDRSPVKA    50
    YPKDPYQEEK WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNASYHRQEV 100
    YVRSTDFDRT LMSAEANLAG LFPPTEVQHF NPNISWQPIP VHTVPITEDR 150
    LLKFPLGPCP RYEQLQNETR QTPEYQNMSI QNAQFLDMVA NETGLMNLTL 200
    ETIWNVYDTL FCEQTHGLLL PPWASPQTVQ ALSQLKDFSF LFLFGIHDQV 250
    QKARLQGGVL LAQILKNLTL MATTSQFPKL LVYSAHDTTL VALQMALNVY 300
    NGKQAPYASC HIFELYQEDN GNFSVEMYFR NDSKKAPWPL TLPGCPHRCP 350
    LQDFLRLTEP VIPKDWQKEC QLASDTADTE VIVALAVCGS ILFLLIVLLL 400
    TVLFRMQAQP PGYHHVADRE DHA 423
    Length:423
    Mass (Da):48,320
    Last modified:February 1, 1991 - v1
    Checksum:i6F4C4819CADD4496
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27893 mRNA. Translation: AAA40744.1.
    PIRiA33395.
    UniGeneiRn.9816.

    Genome annotation databases

    UCSCiRGD:2021. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27893 mRNA. Translation: AAA40744.1 .
    PIRi A33395.
    UniGenei Rn.9816.

    3D structure databases

    ProteinModelPortali P20611.
    SMRi P20611. Positions 31-371.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000018620.

    Proteomic databases

    PaxDbi P20611.
    PRIDEi P20611.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:2021. rat.

    Organism-specific databases

    RGDi 2021. Acp2.

    Phylogenomic databases

    eggNOGi NOG85977.
    HOGENOMi HOG000231439.
    HOVERGENi HBG002203.
    InParanoidi P20611.
    PhylomeDBi P20611.

    Miscellaneous databases

    NextBioi 602461.
    PROi P20611.

    Gene expression databases

    Genevestigatori P20611.

    Family and domain databases

    Gene3Di 3.40.50.1240. 1 hit.
    InterProi IPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    [Graphical view ]
    Pfami PF00328. His_Phos_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53254. SSF53254. 1 hit.
    PROSITEi PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and sequencing of a cDNA clone encoding acid phosphatase in rat liver lysosomes."
      Himeno M., Fujita H., Noguchi Y., Kono A., Kato K.
      Biochem. Biophys. Res. Commun. 162:1044-1053(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Lubec G., Kang S.U., Lubec S.
      Submitted (SEP-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 35-41; 154-161; 255-266 AND 349-364, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.

    Entry informationi

    Entry nameiPPAL_RAT
    AccessioniPrimary (citable) accession number: P20611
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3