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P20611 (PPAL_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Lysosomal acid phosphatase
Short name=LAP
EC=3.1.3.2
Gene names
Name:Acp2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Subcellular location

Lysosome membrane; Single-pass membrane protein; Lumenal side By similarity. Lysosome lumen By similarity. Note: The soluble form arises by proteolytic processing of the membrane-bound form By similarity.

Post-translational modification

The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen By similarity.

Sequence similarities

Belongs to the histidine acid phosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030
Chain31 – 423393Lysosomal acid phosphatase
PRO_0000023962

Regions

Topological domain31 – 380350Lumenal Potential
Transmembrane381 – 40121Helical; Potential
Topological domain402 – 42322Cytoplasmic Potential

Sites

Active site421Nucleophile By similarity
Active site2871Proton donor By similarity

Amino acid modifications

Glycosylation921N-linked (GlcNAc...) Potential
Glycosylation1331N-linked (GlcNAc...) Potential
Glycosylation1671N-linked (GlcNAc...) Potential
Glycosylation1771N-linked (GlcNAc...) Potential
Glycosylation1911N-linked (GlcNAc...) Potential
Glycosylation1971N-linked (GlcNAc...) Potential
Glycosylation2671N-linked (GlcNAc...) Potential
Glycosylation3221N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Disulfide bond159 ↔ 370 By similarity
Disulfide bond212 ↔ 310 By similarity
Disulfide bond345 ↔ 349 By similarity

Sequences

Sequence LengthMass (Da)Tools
P20611 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 6F4C4819CADD4496

FASTA42348,320
        10         20         30         40         50         60 
MAGRQSGWSQ AALLQFLLGM CLMVMPPIQA RSLRFVTLLY RHGDRSPVKA YPKDPYQEEK 

        70         80         90        100        110        120 
WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNASYHRQEV YVRSTDFDRT LMSAEANLAG 

       130        140        150        160        170        180 
LFPPTEVQHF NPNISWQPIP VHTVPITEDR LLKFPLGPCP RYEQLQNETR QTPEYQNMSI 

       190        200        210        220        230        240 
QNAQFLDMVA NETGLMNLTL ETIWNVYDTL FCEQTHGLLL PPWASPQTVQ ALSQLKDFSF 

       250        260        270        280        290        300 
LFLFGIHDQV QKARLQGGVL LAQILKNLTL MATTSQFPKL LVYSAHDTTL VALQMALNVY 

       310        320        330        340        350        360 
NGKQAPYASC HIFELYQEDN GNFSVEMYFR NDSKKAPWPL TLPGCPHRCP LQDFLRLTEP 

       370        380        390        400        410        420 
VIPKDWQKEC QLASDTADTE VIVALAVCGS ILFLLIVLLL TVLFRMQAQP PGYHHVADRE 


DHA

« Hide

References

[1]"Isolation and sequencing of a cDNA clone encoding acid phosphatase in rat liver lysosomes."
Himeno M., Fujita H., Noguchi Y., Kono A., Kato K.
Biochem. Biophys. Res. Commun. 162:1044-1053(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Lubec G., Kang S.U., Lubec S.
Submitted (SEP-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 35-41; 154-161; 255-266 AND 349-364, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M27893 mRNA. Translation: AAA40744.1.
IPIIPI00201276.
PIRA33395.
UniGeneRn.9816.

3D structure databases

ProteinModelPortalP20611.
SMRP20611. Positions 31-371.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000018620.

Proteomic databases

PaxDbP20611.
PRIDEP20611.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:2021. rat.

Organism-specific databases

RGD2021. Acp2.

Phylogenomic databases

eggNOGNOG85977.
HOGENOMHOG000231439.
HOVERGENHBG002203.
InParanoidP20611.
OrthoDBEOG4JQ3XM.

Gene expression databases

ArrayExpressP20611.
GenevestigatorP20611.
GermOnlineENSRNOG00000013594. Rattus norvegicus.

Family and domain databases

InterProIPR000560. His_Pase_superF_clade-2.
[Graphical view]
PfamPF00328. His_Phos_2. 1 hit.
[Graphical view]
PROSITEPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio602461.

Entry information

Entry namePPAL_RAT
AccessionPrimary (citable) accession number: P20611
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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