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P20611

- PPAL_RAT

UniProt

P20611 - PPAL_RAT

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Protein

Lysosomal acid phosphatase

Gene

Acp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei42 – 421NucleophileBy similarity
Active sitei287 – 2871Proton donorBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: RGD
  2. phosphoprotein phosphatase activity Source: RGD
  3. phosphotyrosine binding Source: RGD

GO - Biological processi

  1. autophagic cell death Source: RGD
  2. dephosphorylation Source: GOC
  3. response to organic substance Source: RGD
  4. synaptic transmission Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal acid phosphatase (EC:3.1.3.2)
Short name:
LAP
Gene namesi
Name:Acp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2021. Acp2.

Subcellular locationi

Lysosome membrane By similarity; Single-pass membrane protein By similarity; Lumenal side By similarity. Lysosome lumen By similarity
Note: The soluble form arises by proteolytic processing of the membrane-bound form.By similarity

GO - Cellular componenti

  1. cytoplasmic vesicle Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
  3. lysosome Source: RGD
  4. neuron projection Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Add
BLAST
Chaini31 – 423393Lysosomal acid phosphatasePRO_0000023962Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi159 ↔ 370By similarity
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi212 ↔ 310By similarity
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi345 ↔ 349By similarity

Post-translational modificationi

The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP20611.
PRIDEiP20611.

Expressioni

Gene expression databases

GenevestigatoriP20611.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018620.

Structurei

3D structure databases

ProteinModelPortaliP20611.
SMRiP20611. Positions 31-371.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini31 – 380350LumenalSequence AnalysisAdd
BLAST
Topological domaini402 – 42322CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei381 – 40121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG85977.
HOGENOMiHOG000231439.
HOVERGENiHBG002203.
InParanoidiP20611.
PhylomeDBiP20611.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20611-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGRQSGWSQ AALLQFLLGM CLMVMPPIQA RSLRFVTLLY RHGDRSPVKA
60 70 80 90 100
YPKDPYQEEK WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNASYHRQEV
110 120 130 140 150
YVRSTDFDRT LMSAEANLAG LFPPTEVQHF NPNISWQPIP VHTVPITEDR
160 170 180 190 200
LLKFPLGPCP RYEQLQNETR QTPEYQNMSI QNAQFLDMVA NETGLMNLTL
210 220 230 240 250
ETIWNVYDTL FCEQTHGLLL PPWASPQTVQ ALSQLKDFSF LFLFGIHDQV
260 270 280 290 300
QKARLQGGVL LAQILKNLTL MATTSQFPKL LVYSAHDTTL VALQMALNVY
310 320 330 340 350
NGKQAPYASC HIFELYQEDN GNFSVEMYFR NDSKKAPWPL TLPGCPHRCP
360 370 380 390 400
LQDFLRLTEP VIPKDWQKEC QLASDTADTE VIVALAVCGS ILFLLIVLLL
410 420
TVLFRMQAQP PGYHHVADRE DHA
Length:423
Mass (Da):48,320
Last modified:February 1, 1991 - v1
Checksum:i6F4C4819CADD4496
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27893 mRNA. Translation: AAA40744.1.
PIRiA33395.
UniGeneiRn.9816.

Genome annotation databases

UCSCiRGD:2021. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27893 mRNA. Translation: AAA40744.1 .
PIRi A33395.
UniGenei Rn.9816.

3D structure databases

ProteinModelPortali P20611.
SMRi P20611. Positions 31-371.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000018620.

Proteomic databases

PaxDbi P20611.
PRIDEi P20611.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2021. rat.

Organism-specific databases

RGDi 2021. Acp2.

Phylogenomic databases

eggNOGi NOG85977.
HOGENOMi HOG000231439.
HOVERGENi HBG002203.
InParanoidi P20611.
PhylomeDBi P20611.

Miscellaneous databases

NextBioi 602461.
PROi P20611.

Gene expression databases

Genevestigatori P20611.

Family and domain databases

Gene3Di 3.40.50.1240. 1 hit.
InterProi IPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view ]
Pfami PF00328. His_Phos_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53254. SSF53254. 1 hit.
PROSITEi PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and sequencing of a cDNA clone encoding acid phosphatase in rat liver lysosomes."
    Himeno M., Fujita H., Noguchi Y., Kono A., Kato K.
    Biochem. Biophys. Res. Commun. 162:1044-1053(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 35-41; 154-161; 255-266 AND 349-364, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiPPAL_RAT
AccessioniPrimary (citable) accession number: P20611
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3