P20607 (IL6_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-6
Short name=IL-6

Gene names

Name:	Il6
Synonyms:	Il-6

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the IL-6 superfamily.

Ontologies

Keywords
Biological process	Acute phase
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine Growth factor
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell activation Inferred from electronic annotation. Source: Compara acute-phase response Inferred from electronic annotation. Source: UniProtKB-KW aging Inferred from expression pattern PubMed 19262026. Source: RGD bone remodeling Inferred from expression pattern PubMed 16193234. Source: RGD branching involved in salivary gland morphogenesis Inferred from electronic annotation. Source: Compara cell growth Inferred from direct assay PubMed 20227492. Source: RGD cell redox homeostasis Inferred from mutant phenotype PubMed 7538937. Source: RGD cellular response to hydrogen peroxide Inferred from electronic annotation. Source: Compara defense response to Gram-negative bacterium Inferred from electronic annotation. Source: Compara defense response to Gram-positive bacterium Inferred from electronic annotation. Source: Compara defense response to protozoan Inferred from electronic annotation. Source: Compara defense response to virus Inferred from electronic annotation. Source: Compara endocrine pancreas development Inferred from electronic annotation. Source: Compara epithelial cell proliferation involved in salivary gland morphogenesis Inferred from electronic annotation. Source: Compara glucagon secretion Inferred from electronic annotation. Source: Compara immune response Inferred from electronic annotation. Source: InterPro interleukin-6-mediated signaling pathway Inferred from electronic annotation. Source: Compara muscle cell homeostasis Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Inferred from mutant phenotype PubMed 9645688. Source: RGD negative regulation of chemokine biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of collagen biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from direct assay PubMed 19061939. Source: RGD negative regulation of cytokine secretion Inferred from direct assay PubMed 19057004. Source: RGD negative regulation of gluconeogenesis Inferred from direct assay PubMed 16308834. Source: RGD negative regulation of hormone secretion Inferred from electronic annotation. Source: Compara negative regulation of muscle organ development Inferred from direct assay PubMed 19057004. Source: RGD negative regulation of neuron death Inferred from direct assay PubMed 19061939. Source: RGD negative regulation of protein kinase activity Inferred from direct assay PubMed 15845578. Source: RGD neuron projection development Inferred from electronic annotation. Source: Compara neutrophil apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of B cell activation Inferred from electronic annotation. Source: Compara positive regulation of DNA replication Inferred from mutant phenotype PubMed 15828022. Source: RGD positive regulation of ERK1 and ERK2 cascade Inferred from direct assay PubMed 19061939PubMed 20227492. Source: RGD positive regulation of T cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of T-helper 2 cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of acute inflammatory response Inferred from electronic annotation. Source: Compara positive regulation of chemokine production Inferred from electronic annotation. Source: Compara positive regulation of epithelial cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of gene expression Inferred from direct assay PubMed 19057004. Source: RGD positive regulation of immunoglobulin secretion Inferred from electronic annotation. Source: Compara positive regulation of interleukin-6 production Inferred from electronic annotation. Source: Compara positive regulation of neuron differentiation Inferred from mutant phenotype PubMed 7538937. Source: RGD positive regulation of nitric oxide biosynthetic process Inferred from direct assay PubMed 15845578. Source: RGD positive regulation of peptidyl-serine phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of protein import into nucleus, translocation Inferred from direct assay PubMed 12015387. Source: RGD positive regulation of protein kinase B signaling cascade Inferred from direct assay PubMed 20227492. Source: RGD positive regulation of sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of translation Inferred from electronic annotation. Source: Compara positive regulation of transmission of nerve impulse Inferred from direct assay PubMed 19258490. Source: RGD positive regulation of tyrosine phosphorylation of Stat3 protein Inferred from direct assay PubMed 20227492. Source: RGD regulation of cell shape Inferred from direct assay PubMed 15845578. Source: RGD regulation of circadian sleep/wake cycle, non-REM sleep Inferred from mutant phenotype PubMed 12667648. Source: RGD regulation of vascular endothelial growth factor production Inferred from electronic annotation. Source: Compara response to amino acid stimulus Inferred from expression pattern PubMed 19344884. Source: RGD response to antibiotic Inferred from expression pattern PubMed 19401694. Source: RGD response to caffeine Inferred from expression pattern PubMed 15845578. Source: RGD response to calcium ion Inferred from expression pattern PubMed 15845578. Source: RGD response to cold Inferred from expression pattern PubMed 19406216. Source: RGD response to cytokine stimulus Inferred from expression pattern PubMed 19395591. Source: RGD response to drug Inferred from expression pattern PubMed 19413671. Source: RGD response to electrical stimulus Inferred from expression pattern PubMed 19410369. Source: RGD response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara response to heat Inferred from expression pattern PubMed 19365172. Source: RGD response to insulin stimulus Inferred from expression pattern PubMed 19373130. Source: RGD response to lipopolysaccharide Inferred from expression pattern PubMed 19410080. Source: RGD response to mechanical stimulus Inferred from expression pattern PubMed 11834488. Source: RGD response to nutrient levels Inferred from expression pattern PubMed 19374947. Source: RGD response to peptidoglycan Inferred from electronic annotation. Source: Compara response to wounding Inferred from expression pattern PubMed 19394966. Source: RGD
Cellular_component	external side of plasma membrane Inferred from electronic annotation. Source: Compara extracellular space Inferred from direct assay PubMed 19406831. Source: RGD interleukin-6 receptor complex Inferred from electronic annotation. Source: Compara
Molecular_function	cytokine activity Inferred from direct assay PubMed 16413512. Source: RGD interleukin-6 receptor binding Inferred from direct assay PubMed 12604335PubMed 16413512. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 211	187	Interleukin-6		PRO_0000015593
Amino acid modifications
	Disulfide bond	70 ↔ 76		By similarity
	Disulfide bond	99 ↔ 109		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20607 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 17D248A14F96B5C1	FASTA	211	24,357
	10 20 30 40 50 60 MKFLSARDFQ PVAFLGLMLL TATAFPTSQV RRGDFTEDTT HNRPVYTTSQ VGGLITYVLR 70 80 90 100 110 120 EILEMRKELC NGNSDCMNSD DALSENNLKL PEIQRNDGCF QTGYNQEICL LKICSGLLEF 130 140 150 160 170 180 RFYLEFVKNN LQDNKKDKAR VIQSNTETLV HIFKQEIKDS YKIVLPTPTS NALLMEKLES 190 200 210 QKEWLRTKTI QLILKALEEF LKVTMRSTRQ T

References

[1]	"Structure of the rat interleukin 6 gene and its expression in macrophage-derived cells." Northemann W., Braciak T.A., Hattori M., Lee F., Fey G.H. J. Biol. Chem. 264:16072-16082(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M26744 mRNA. Translation: AAA77659.1. M26745 Genomic DNA. Translation: AAA41430.1.
IPI	IPI00201260.
PIR	A34247.
RefSeq	NP_036721.1. NM_012589.2.
UniGene	Rn.9873.
3D structure databases
ProteinModelPortal	P20607.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-189606.
STRING	10116.ENSRNOP00000013732.
Proteomic databases
PRIDE	P20607.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000013732; ENSRNOP00000013732; ENSRNOG00000010278.
GeneID	24498.
KEGG	rno:24498.
UCSC	RGD:2901. rat.
Organism-specific databases
CTD	3569.
RGD	2901. Il6.
Phylogenomic databases
eggNOG	NOG48083.
GeneTree	ENSGT00390000000878.
HOGENOM	HOG000236330.
HOVERGEN	HBG000471.
InParanoid	P20607.
KO	K05405.
OrthoDB	EOG44J2KH.
Gene expression databases
ArrayExpress	P20607.
Genevestigator	P20607.
GermOnline	ENSRNOG00000010278. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR003573. IL6_MGF_GCSF. IPR003574. Interleukin_6. [Graphical view]
PANTHER	PTHR11457. PTHR11457. 1 hit.
Pfam	PF00489. IL6. 1 hit. [Graphical view]
PIRSF	PIRSF001935. IL6_MGF_GCSF. 1 hit.
PRINTS	PR00433. IL6GCSFMGF. PR00434. INTERLEUKIN6.
SMART	SM00126. IL6. 1 hit. [Graphical view]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00254. INTERLEUKIN_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603495.

Entry information

Entry name

IL6_RAT

Accession

Primary (citable) accession number: P20607

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 29, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program