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Protein

Small COPII coat GTPase SAR1

Gene

SAR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Small GTPase component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules. SAR1 controls the coat assembly in a stepwise manner. Activated SAR1-GTP by SEC12 binds to membranes first and recruits the SEC23/24 complex. These SEC23/24-SAR1 prebudding intermediates are then collected by the SEC13/31 complex as subunits polymerize to form coated transport vesicles. Conversion to SAR1-GDP triggers coat release and recycles COPII subunits.14 Publications

Catalytic activityi

GTP + H2O = GDP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 37GTP8
Nucleotide bindingi73 – 76GTP4
Nucleotide bindingi132 – 135GTP4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • intracellular protein transport Source: InterPro
  • mitochondrial fission Source: SGD
  • mitochondrial membrane organization Source: SGD
  • regulation of COPII vesicle coating Source: SGD
  • single-organism membrane organization Source: SGD
  • vesicle-mediated transport Source: UniProtKB-KW
  • vesicle organization Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processER-Golgi transport, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34107-MONOMER.
ReactomeiR-SCE-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-SCE-5694530. Cargo concentration in the ER.
R-SCE-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Names & Taxonomyi

Protein namesi
Recommended name:
Small COPII coat GTPase SAR1 (EC:3.6.5.-)
Alternative name(s):
GTP-binding protein SAR1
Secretion-associated RAS-related protein 1
Gene namesi
Name:SAR1
Ordered Locus Names:YPL218W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL218W.
SGDiS000006139. SAR1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi32D → G: No cell growth at 37 degrees Celsius. Decreases GTP binding and ER-to-Golgi vesicle transport. 3 Publications1
Mutagenesisi36K → M: No cell growth at 23 or 37 degrees Celsius. 1 Publication1
Mutagenesisi54T → A: No cell growth at 23 or 37 degrees Celsius. Decreases GTP binding and impairs ER-to-Golgi vesicle transport. 2 Publications1
Mutagenesisi73D → V: No cell growth at 23 or 37 degrees Celsius. 1 Publication1
Mutagenesisi77H → L: No cell growth at 23 or 37 degrees Celsius. Impairs ER-to-Golgi vesicle transport. 2 Publications1
Mutagenesisi112E → K: Decreases guanine nucleotide binding. 2 Publications1
Mutagenesisi132N → I: No cell growth at 37 degrees Celsius. 2 Publications1
Mutagenesisi171C → S: Normal cell growth. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002062751 – 190Small COPII coat GTPase SAR1Add BLAST190

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei155PhosphothreonineCombined sources1
Modified residuei157PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP20606.
PRIDEiP20606.

PTM databases

iPTMnetiP20606.

Interactioni

Subunit structurei

COPII is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. Interacts with EMP24.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CRN1Q064402EBI-16472,EBI-4950

Protein-protein interaction databases

BioGridi35967. 58 interactors.
DIPiDIP-2231N.
IntActiP20606. 13 interactors.
MINTiMINT-479143.
STRINGi4932.YPL218W.

Structurei

Secondary structure

1190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi25 – 31Combined sources7
Helixi36 – 45Combined sources10
Beta strandi58 – 64Combined sources7
Beta strandi67 – 73Combined sources7
Helixi78 – 80Combined sources3
Helixi84 – 87Combined sources4
Beta strandi93 – 99Combined sources7
Helixi103 – 105Combined sources3
Helixi106 – 117Combined sources12
Helixi120 – 122Combined sources3
Beta strandi127 – 132Combined sources6
Beta strandi136 – 138Combined sources3
Helixi142 – 148Combined sources7
Beta strandi166 – 170Combined sources5
Turni173 – 176Combined sources4
Helixi179 – 187Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M2OX-ray2.50B/D1-190[»]
2QTVX-ray2.50B23-189[»]
4BZIelectron microscopy23.00B/J/K1-190[»]
ProteinModelPortaliP20606.
SMRiP20606.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20606.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. SAR1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074696.
HOGENOMiHOG000163690.
InParanoidiP20606.
KOiK07953.
OMAiKDDRLAQ.
OrthoDBiEOG092C508L.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR006689. Small_GTPase_ARF/SAR.
IPR006687. Small_GTPase_SAR1.
PfamiView protein in Pfam
PF00025. Arf. 1 hit.
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51422. SAR1. 1 hit.

Sequencei

Sequence statusi: Complete.

P20606-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGWDIFGWF RDVLASLGLW NKHGKLLFLG LDNAGKTTLL HMLKNDRLAT
60 70 80 90 100
LQPTWHPTSE ELAIGNIKFT TFDLGGHIQA RRLWKDYFPE VNGIVFLVDA
110 120 130 140 150
ADPERFDEAR VELDALFNIA ELKDVPFVIL GNKIDAPNAV SEAELRSALG
160 170 180 190
LLNTTGSQRI EGQRPVEVFM CSVVMRNGYL EAFQWLSQYI
Length:190
Mass (Da):21,451
Last modified:February 1, 1991 - v1
Checksum:iE2348B57896B5A13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51667 Genomic DNA. Translation: CAA35978.1.
Z73574 Genomic DNA. Translation: CAA97933.1.
BK006949 Genomic DNA. Translation: DAA11218.1.
PIRiA33619.
RefSeqiNP_015106.1. NM_001184032.1.

Genome annotation databases

EnsemblFungiiYPL218W; YPL218W; YPL218W.
GeneIDi855883.
KEGGisce:YPL218W.

Similar proteinsi

Entry informationi

Entry nameiSAR1_YEAST
AccessioniPrimary (citable) accession number: P20606
Secondary accession number(s): D6W3F2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 27, 2017
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names