P20605 (FIC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable adenosine monophosphate-protein transferase fic
EC=2.7.7.n1

Alternative name(s):
Cell filamentation protein fic

Gene names

Name:	fic
Ordered Locus Names:	b3361, JW3324

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins By similarity. Involved in cell filamentation induced by cyclic AMP. May have some role in cell division.
Catalytic activity	ATP + [protein] = diphosphate + [protein]-AMP.
Sequence similarities	Belongs to the fic family. Contains 1 fido domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotidyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
modB	P0AF01	1	EBI-1132602,EBI-1115113

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Probable adenosine monophosphate-protein transferase fic		PRO_0000087240
Regions
	Domain	54 – 195	142	Fido
	Nucleotide binding	84 – 85	2	ATP By similarity
	Nucleotide binding	140 – 142	3	ATP By similarity
Sites
	Binding site	146	1	ATP By similarity
	Binding site	172	1	ATP By similarity
Experimental info
	Mutagenesis	55	1	G → R: Inhibitory effect on cell division induced by cAMP with temperature sensitivity. Ref.1
	Sequence conflict	53 – 54	2	VR → GA in AAA23773. Ref.1
	Sequence conflict	149	1	Missing in AAA24263. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P20605 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 3E7E0B034BEBC70C	FASTA	200	22,960
	10 20 30 40 50 60 MSDKFGEGRD PYLYPGLDIM RNRLNIRQQQ RLEQAAYEMT ALRAATIELG PLVRGLPHLR 70 80 90 100 110 120 TIHRQLYQDI FDWAGQLREV DIYQGDTPFC HFAYIEKEGN ALMQDLEEEG YLVGLEKAKF 130 140 150 160 170 180 VERLAHYYCE INVLHPFRVG SGLAQRIFFE QLAIHAGYQL SWQGIEKEAW NQANQSGAMG 190 200 DLTALQMIFS KVVSEAGESE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequences of fic and fic-1 genes involved in cell filamentation induced by cyclic AMP in Escherichia coli." Kawamukai M., Matsuda H., Fujii W., Utsumi R., Komano T. J. Bacteriol. 171:4525-4529(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-55.
[2]	"Chromosomal organization and expression of Escherichia coli pabA." Tran P.V., Bannor T.A., Doktor S.Z., Nichols B.P. J. Bacteriol. 172:397-410(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Mutational analysis of the fic promoter recognized by RpoS (sigma 38) in Escherichia coli." Utsumi R., Nakayama T., Iwamoto N., Kohda K., Kawamukai M., Tanabe H., Tanaka K., Takahashi H., Noda M. Biosci. Biotechnol. Biochem. 59:1573-1575(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18.
[6]	"Two distinct forms of peptidylprolyl-cis-trans-isomerase are expressed separately in periplasmic and cytoplasmic compartments of Escherichia coli cells." Hayano T., Takahashi N., Kato S., Maki N., Suzuki M. Biochemistry 30:3041-3048(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M28363 Genomic DNA. Translation: AAA23773.1. M32354 Genomic DNA. Translation: AAA24263.1. U18997 Genomic DNA. Translation: AAA58158.1. U00096 Genomic DNA. Translation: AAC76386.1. AP009048 Genomic DNA. Translation: BAE77929.1. S79599 Genomic DNA. Translation: AAB35351.1. M55429 Genomic DNA. Translation: AAA23452.1.
PIR	D65130.
RefSeq	NP_417820.1. NC_000913.2. YP_492070.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P20605.
SMR	P20605. Positions 4-195.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9608N.
IntAct	P20605. 1 interaction.
STRING	511145.b3361.
Proteomic databases
PRIDE	P20605.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76386; AAC76386; b3361. BAE77929; BAE77929; BAE77929.
GeneID	12932089. 947872.
KEGG	ecj:Y75_p3814. eco:b3361.
PATRIC	32122156. VBIEscCol129921_3455.
Organism-specific databases
EchoBASE	EB0303.
EcoGene	EG10307. fic.
Phylogenomic databases
eggNOG	COG2184.
HOGENOM	HOG000125441.
KO	K04095.
OMA	LHPFRIG.
ProtClustDB	PRK10347.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10307-MONOMER. ECOL316407:JW3324-MONOMER.
Gene expression databases
Genevestigator	P20605.
Family and domain databases
InterPro	IPR003812. Fido. [Graphical view]
Pfam	PF02661. Fic. 1 hit. [Graphical view]
SUPFAM	SSF140931. SSF140931. 1 hit.
PROSITE	PS51459. FIDO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FIC_ECOLI

Accession

Primary (citable) accession number: P20605
Secondary accession number(s): Q2M727

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program