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Protein

Probable adenosine monophosphate-protein transferase fic

Gene

fic

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (By similarity). Involved in cell filamentation induced by cyclic AMP. May have some role in cell division.By similarity

Catalytic activityi

ATP + [protein] = diphosphate + [protein]-AMP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei146ATPBy similarity1
Binding sitei172ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi84 – 85ATPBy similarity2
Nucleotide bindingi140 – 142ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • regulation of cell division Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10307-MONOMER.
ECOL316407:JW3324-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable adenosine monophosphate-protein transferase fic (EC:2.7.7.n1)
Alternative name(s):
Cell filamentation protein fic
Gene namesi
Name:fic
Ordered Locus Names:b3361, JW3324
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10307. fic.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi55G → R: Inhibitory effect on cell division induced by cAMP with temperature sensitivity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000872401 – 200Probable adenosine monophosphate-protein transferase ficAdd BLAST200

Proteomic databases

PaxDbiP20605.
PRIDEiP20605.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaXP067102EBI-1132602,EBI-549140

Protein-protein interaction databases

BioGridi4261083. 52 interactors.
DIPiDIP-9608N.
IntActiP20605. 5 interactors.
STRINGi511145.b3361.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JFFX-ray2.00A/C1-200[»]
5JFZX-ray2.40A/C/E3-200[»]
ProteinModelPortaliP20605.
SMRiP20605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 195FidoPROSITE-ProRule annotationAdd BLAST142

Sequence similaritiesi

Belongs to the fic family.Curated
Contains 1 fido domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105MZU. Bacteria.
COG2184. LUCA.
HOGENOMiHOG000125441.
InParanoidiP20605.
KOiK04095.
OMAiVLHPFRI.
PhylomeDBiP20605.

Family and domain databases

Gene3Di1.10.3290.10. 1 hit.
InterProiIPR003812. Fido.
[Graphical view]
PfamiPF02661. Fic. 1 hit.
[Graphical view]
SUPFAMiSSF140931. SSF140931. 1 hit.
PROSITEiPS51459. FIDO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKFGEGRD PYLYPGLDIM RNRLNIRQQQ RLEQAAYEMT ALRAATIELG
60 70 80 90 100
PLVRGLPHLR TIHRQLYQDI FDWAGQLREV DIYQGDTPFC HFAYIEKEGN
110 120 130 140 150
ALMQDLEEEG YLVGLEKAKF VERLAHYYCE INVLHPFRVG SGLAQRIFFE
160 170 180 190 200
QLAIHAGYQL SWQGIEKEAW NQANQSGAMG DLTALQMIFS KVVSEAGESE
Length:200
Mass (Da):22,960
Last modified:February 1, 1991 - v1
Checksum:i3E7E0B034BEBC70C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53 – 54VR → GA in AAA23773 (PubMed:2546924).Curated2
Sequence conflicti149Missing in AAA24263 (PubMed:2403545).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28363 Genomic DNA. Translation: AAA23773.1.
M32354 Genomic DNA. Translation: AAA24263.1.
U18997 Genomic DNA. Translation: AAA58158.1.
U00096 Genomic DNA. Translation: AAC76386.1.
AP009048 Genomic DNA. Translation: BAE77929.1.
S79599 Genomic DNA. Translation: AAB35351.1.
M55429 Genomic DNA. Translation: AAA23452.1.
PIRiD65130.
RefSeqiNP_417820.1. NC_000913.3.
WP_001280641.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76386; AAC76386; b3361.
BAE77929; BAE77929; BAE77929.
GeneIDi947872.
KEGGiecj:JW3324.
eco:b3361.
PATRICi32122156. VBIEscCol129921_3455.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28363 Genomic DNA. Translation: AAA23773.1.
M32354 Genomic DNA. Translation: AAA24263.1.
U18997 Genomic DNA. Translation: AAA58158.1.
U00096 Genomic DNA. Translation: AAC76386.1.
AP009048 Genomic DNA. Translation: BAE77929.1.
S79599 Genomic DNA. Translation: AAB35351.1.
M55429 Genomic DNA. Translation: AAA23452.1.
PIRiD65130.
RefSeqiNP_417820.1. NC_000913.3.
WP_001280641.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JFFX-ray2.00A/C1-200[»]
5JFZX-ray2.40A/C/E3-200[»]
ProteinModelPortaliP20605.
SMRiP20605.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261083. 52 interactors.
DIPiDIP-9608N.
IntActiP20605. 5 interactors.
STRINGi511145.b3361.

Proteomic databases

PaxDbiP20605.
PRIDEiP20605.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76386; AAC76386; b3361.
BAE77929; BAE77929; BAE77929.
GeneIDi947872.
KEGGiecj:JW3324.
eco:b3361.
PATRICi32122156. VBIEscCol129921_3455.

Organism-specific databases

EchoBASEiEB0303.
EcoGeneiEG10307. fic.

Phylogenomic databases

eggNOGiENOG4105MZU. Bacteria.
COG2184. LUCA.
HOGENOMiHOG000125441.
InParanoidiP20605.
KOiK04095.
OMAiVLHPFRI.
PhylomeDBiP20605.

Enzyme and pathway databases

BioCyciEcoCyc:EG10307-MONOMER.
ECOL316407:JW3324-MONOMER.

Miscellaneous databases

PROiP20605.

Family and domain databases

Gene3Di1.10.3290.10. 1 hit.
InterProiIPR003812. Fido.
[Graphical view]
PfamiPF02661. Fic. 1 hit.
[Graphical view]
SUPFAMiSSF140931. SSF140931. 1 hit.
PROSITEiPS51459. FIDO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFIC_ECOLI
AccessioniPrimary (citable) accession number: P20605
Secondary accession number(s): Q2M727
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.