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Protein

Serine/threonine-protein phosphatase PP1-1

Gene

SIT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the dephosphorylation of the large subunit of RNA polymerase II. Is required in late G1 for normal G1 cyclin expression, bud initiation and expression of certain genes that are periodically expressed during late G1. Associates with the SAP proteins in a cell cycle-dependent manner.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Manganese 1By similarity
Metal bindingi55 – 551Manganese 1By similarity
Metal bindingi82 – 821Manganese 1By similarity
Metal bindingi82 – 821Manganese 2By similarity
Metal bindingi114 – 1141Manganese 2By similarity
Active sitei115 – 1151Proton donorBy similarity
Metal bindingi164 – 1641Manganese 2By similarity
Metal bindingi238 – 2381Manganese 2By similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  • actin cytoskeleton organization Source: SGD
  • cell division Source: UniProtKB-KW
  • cellular response to oxidative stress Source: SGD
  • dephosphorylation Source: SGD
  • DNA repair Source: SGD
  • fungal-type cell wall organization Source: SGD
  • G1/S transition of mitotic cell cycle Source: SGD
  • intracellular signal transduction Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • replicative cell aging Source: SGD
  • TOR signaling Source: SGD
  • tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29466-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1-1 (EC:3.1.3.16)
Gene namesi
Name:SIT4
Synonyms:PPH1
Ordered Locus Names:YDL047W
ORF Names:D2693
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL047W.
SGDiS000002205. SIT4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • protein phosphatase type 2A complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi35 – 351L → A: Reduced interaction with TAP42. 1 Publication
Mutagenesisi37 – 382EE → AA: Nearly no interaction with TAP42. 1 Publication
Mutagenesisi38 – 381E → A: Normal interaction with TAP42. 1 Publication
Mutagenesisi40 – 401N → A: Normal interaction with TAP42. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Serine/threonine-protein phosphatase PP1-1PRO_0000058881Add
BLAST

Proteomic databases

MaxQBiP20604.
PRIDEiP20604.

PTM databases

iPTMnetiP20604.

Expressioni

Developmental stagei

Functions in the late cell cycle G1 phase for progression into the S phase, possibly associated in two separate complexes with the phosphorylated forms of p155 and p190, two high MW proteins.

Interactioni

Subunit structurei

Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Forms a ternary complex with RRD1-TAP42.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RRD1P404544EBI-13707,EBI-25278
SAP155P436128EBI-13707,EBI-16370
SAP185P408568EBI-13707,EBI-16384
SAP190P361234EBI-13707,EBI-16392
TAP42Q043726EBI-13707,EBI-18926

Protein-protein interaction databases

BioGridi32011. 179 interactions.
DIPiDIP-5850N.
IntActiP20604. 41 interactions.
MINTiMINT-615434.

Structurei

3D structure databases

ProteinModelPortaliP20604.
SMRiP20604. Positions 7-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074961.
HOGENOMiHOG000172696.
InParanoidiP20604.
KOiK15427.
OMAiKYHFDEN.
OrthoDBiEOG092C2R40.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSRGPDEWL ETIKKCQALT ENEMKQLCEM VKELLMEESN IQPVQTPVTV
60 70 80 90 100
CGDIHGQFHD LLELFRTAGG FPDDINYIFL GDYVDRGYYS LETFTLLMCL
110 120 130 140 150
KVKYPAKITL VRGNHESRQI TQVYGFYEEC LNKYGSTTVW KYCCQVFDFL
160 170 180 190 200
TLAAIIDGKI LCVHGGLSPE IRMLDQIRVL SRAQEVPHEG GFSDLLWSDP
210 220 230 240 250
DNVEAWQVSP RGAGWLFGSK VAREFNHVNG LNLIARAHQL VMEGFKYHFP
260 270 280 290 300
EKDVVTVWSA PNYCYRCGNV ASVMKVDEDL EPTFKIFSAV PDDYIRESTA
310
NHNNQRAGYF L
Length:311
Mass (Da):35,538
Last modified:February 1, 1991 - v1
Checksum:iAF52BC65E4E715EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24395 Genomic DNA. Translation: AAA56864.1.
Z71781 Genomic DNA. Translation: CAA96442.1.
Z74095 Genomic DNA. Translation: CAA98609.1.
BK006938 Genomic DNA. Translation: DAA11808.1.
PIRiA31874. PABY1.
RefSeqiNP_010236.1. NM_001180106.1.

Genome annotation databases

EnsemblFungiiYDL047W; YDL047W; YDL047W.
GeneIDi851513.
KEGGisce:YDL047W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24395 Genomic DNA. Translation: AAA56864.1.
Z71781 Genomic DNA. Translation: CAA96442.1.
Z74095 Genomic DNA. Translation: CAA98609.1.
BK006938 Genomic DNA. Translation: DAA11808.1.
PIRiA31874. PABY1.
RefSeqiNP_010236.1. NM_001180106.1.

3D structure databases

ProteinModelPortaliP20604.
SMRiP20604. Positions 7-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32011. 179 interactions.
DIPiDIP-5850N.
IntActiP20604. 41 interactions.
MINTiMINT-615434.

PTM databases

iPTMnetiP20604.

Proteomic databases

MaxQBiP20604.
PRIDEiP20604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL047W; YDL047W; YDL047W.
GeneIDi851513.
KEGGisce:YDL047W.

Organism-specific databases

EuPathDBiFungiDB:YDL047W.
SGDiS000002205. SIT4.

Phylogenomic databases

GeneTreeiENSGT00550000074961.
HOGENOMiHOG000172696.
InParanoidiP20604.
KOiK15427.
OMAiKYHFDEN.
OrthoDBiEOG092C2R40.

Enzyme and pathway databases

BioCyciYEAST:G3O-29466-MONOMER.

Miscellaneous databases

PROiP20604.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP11_YEAST
AccessioniPrimary (citable) accession number: P20604
Secondary accession number(s): D6VRU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 7, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3970 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.