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P20604

- PP11_YEAST

UniProt

P20604 - PP11_YEAST

Protein

Serine/threonine-protein phosphatase PP1-1

Gene

SIT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Involved in the dephosphorylation of the large subunit of RNA polymerase II. Is required in late G1 for normal G1 cyclin expression, bud initiation and expression of certain genes that are periodically expressed during late G1. Associates with the SAP proteins in a cell cycle-dependent manner.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi53 – 531Manganese 1By similarity
    Metal bindingi55 – 551Manganese 1By similarity
    Metal bindingi82 – 821Manganese 1By similarity
    Metal bindingi82 – 821Manganese 2By similarity
    Metal bindingi114 – 1141Manganese 2By similarity
    Active sitei115 – 1151Proton donorBy similarity
    Metal bindingi164 – 1641Manganese 2By similarity
    Metal bindingi238 – 2381Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    1. actin cytoskeleton organization Source: SGD
    2. cellular response to oxidative stress Source: SGD
    3. dephosphorylation Source: SGD
    4. DNA repair Source: SGD
    5. fungal-type cell wall organization Source: SGD
    6. G1/S transition of mitotic cell cycle Source: SGD
    7. intracellular signal transduction Source: SGD
    8. mitotic nuclear division Source: UniProtKB-KW
    9. replicative cell aging Source: SGD
    10. TOR signaling Source: SGD
    11. tRNA wobble uridine modification Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29466-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP1-1 (EC:3.1.3.16)
    Gene namesi
    Name:SIT4
    Synonyms:PPH1
    Ordered Locus Names:YDL047W
    ORF Names:D2693
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL047w.
    SGDiS000002205. SIT4.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi35 – 351L → A: Reduced interaction with TAP42. 1 Publication
    Mutagenesisi37 – 382EE → AA: Nearly no interaction with TAP42.
    Mutagenesisi38 – 381E → A: Normal interaction with TAP42. 1 Publication
    Mutagenesisi40 – 401N → A: Normal interaction with TAP42. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 311311Serine/threonine-protein phosphatase PP1-1PRO_0000058881Add
    BLAST

    Proteomic databases

    MaxQBiP20604.
    PaxDbiP20604.
    PeptideAtlasiP20604.
    PRIDEiP20604.

    Expressioni

    Developmental stagei

    Functions in the late cell cycle G1 phase for progression into the S phase, possibly associated in two separate complexes with the phosphorylated forms of p155 and p190, two high MW proteins.

    Gene expression databases

    GenevestigatoriP20604.

    Interactioni

    Subunit structurei

    Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Forms a ternary complex with RRD1-TAP42.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RRD1P404544EBI-13707,EBI-25278
    SAP155P436128EBI-13707,EBI-16370
    SAP185P408568EBI-13707,EBI-16384
    SAP190P361234EBI-13707,EBI-16392
    TAP42Q043726EBI-13707,EBI-18926

    Protein-protein interaction databases

    BioGridi32011. 169 interactions.
    DIPiDIP-5850N.
    IntActiP20604. 40 interactions.
    MINTiMINT-615434.
    STRINGi4932.YDL047W.

    Structurei

    3D structure databases

    ProteinModelPortaliP20604.
    SMRiP20604. Positions 7-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00550000074961.
    HOGENOMiHOG000172696.
    KOiK15427.
    OMAiQKDSAYT.
    OrthoDBiEOG7FFN29.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20604-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSRGPDEWL ETIKKCQALT ENEMKQLCEM VKELLMEESN IQPVQTPVTV    50
    CGDIHGQFHD LLELFRTAGG FPDDINYIFL GDYVDRGYYS LETFTLLMCL 100
    KVKYPAKITL VRGNHESRQI TQVYGFYEEC LNKYGSTTVW KYCCQVFDFL 150
    TLAAIIDGKI LCVHGGLSPE IRMLDQIRVL SRAQEVPHEG GFSDLLWSDP 200
    DNVEAWQVSP RGAGWLFGSK VAREFNHVNG LNLIARAHQL VMEGFKYHFP 250
    EKDVVTVWSA PNYCYRCGNV ASVMKVDEDL EPTFKIFSAV PDDYIRESTA 300
    NHNNQRAGYF L 311
    Length:311
    Mass (Da):35,538
    Last modified:February 1, 1991 - v1
    Checksum:iAF52BC65E4E715EC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24395 Genomic DNA. Translation: AAA56864.1.
    Z71781 Genomic DNA. Translation: CAA96442.1.
    Z74095 Genomic DNA. Translation: CAA98609.1.
    BK006938 Genomic DNA. Translation: DAA11808.1.
    PIRiA31874. PABY1.
    RefSeqiNP_010236.1. NM_001180106.1.

    Genome annotation databases

    EnsemblFungiiYDL047W; YDL047W; YDL047W.
    GeneIDi851513.
    KEGGisce:YDL047W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24395 Genomic DNA. Translation: AAA56864.1 .
    Z71781 Genomic DNA. Translation: CAA96442.1 .
    Z74095 Genomic DNA. Translation: CAA98609.1 .
    BK006938 Genomic DNA. Translation: DAA11808.1 .
    PIRi A31874. PABY1.
    RefSeqi NP_010236.1. NM_001180106.1.

    3D structure databases

    ProteinModelPortali P20604.
    SMRi P20604. Positions 7-291.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32011. 169 interactions.
    DIPi DIP-5850N.
    IntActi P20604. 40 interactions.
    MINTi MINT-615434.
    STRINGi 4932.YDL047W.

    Proteomic databases

    MaxQBi P20604.
    PaxDbi P20604.
    PeptideAtlasi P20604.
    PRIDEi P20604.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL047W ; YDL047W ; YDL047W .
    GeneIDi 851513.
    KEGGi sce:YDL047W.

    Organism-specific databases

    CYGDi YDL047w.
    SGDi S000002205. SIT4.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00550000074961.
    HOGENOMi HOG000172696.
    KOi K15427.
    OMAi QKDSAYT.
    OrthoDBi EOG7FFN29.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29466-MONOMER.

    Miscellaneous databases

    NextBioi 968878.
    PROi P20604.

    Gene expression databases

    Genevestigatori P20604.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatases."
      Arndt K.T., Styles C.A., Fink G.R.
      Cell 56:527-537(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The SIT4 protein phosphatase functions in late G1 for progression into S phase."
      Sutton A., Immanuel D., Arndt K.T.
      Mol. Cell. Biol. 11:2133-2148(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases."
      Wang H., Wang X., Jiang Y.
      Mol. Biol. Cell 14:4342-4351(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TAP42, MUTAGENESIS OF LEU-35; 37-GLU-GLU-38; GLU-38 AND ASN-40.
    6. "Multiple roles of Tap42 in mediating rapamycin-induced transcriptional changes in yeast."
      Duevel K., Santhanam A., Garrett S., Schneper L., Broach J.R.
      Mol. Cell 11:1467-1478(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TAP42.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2."
      Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J.
      Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PPE1 AND RRD1.
    9. "The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes."
      Zheng Y., Jiang Y.
      Mol. Biol. Cell 16:2119-2127(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RRD1 AND TAP42.

    Entry informationi

    Entry nameiPP11_YEAST
    AccessioniPrimary (citable) accession number: P20604
    Secondary accession number(s): D6VRU8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 142 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3970 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3