SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P20604

- PP11_YEAST

UniProt

P20604 - PP11_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Serine/threonine-protein phosphatase PP1-1

Gene
SIT4, PPH1, YDL047W, D2693
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the dephosphorylation of the large subunit of RNA polymerase II. Is required in late G1 for normal G1 cyclin expression, bud initiation and expression of certain genes that are periodically expressed during late G1. Associates with the SAP proteins in a cell cycle-dependent manner.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Manganese 1 By similarity
Metal bindingi55 – 551Manganese 1 By similarity
Metal bindingi82 – 821Manganese 1 By similarity
Metal bindingi82 – 821Manganese 2 By similarity
Metal bindingi114 – 1141Manganese 2 By similarity
Active sitei115 – 1151Proton donor By similarity
Metal bindingi164 – 1641Manganese 2 By similarity
Metal bindingi238 – 2381Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein binding Source: IntAct
  3. protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  1. actin cytoskeleton organization Source: SGD
  2. cellular response to oxidative stress Source: SGD
  3. dephosphorylation Source: SGD
  4. DNA repair Source: SGD
  5. fungal-type cell wall organization Source: SGD
  6. G1/S transition of mitotic cell cycle Source: SGD
  7. intracellular signal transduction Source: SGD
  8. mitotic nuclear division Source: UniProtKB-KW
  9. replicative cell aging Source: SGD
  10. TOR signaling Source: SGD
  11. tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29466-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1-1 (EC:3.1.3.16)
Gene namesi
Name:SIT4
Synonyms:PPH1
Ordered Locus Names:YDL047W
ORF Names:D2693
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL047w.
SGDiS000002205. SIT4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi35 – 351L → A: Reduced interaction with TAP42. 1 Publication
Mutagenesisi37 – 382EE → AA: Nearly no interaction with TAP42. 1 Publication
Mutagenesisi38 – 381E → A: Normal interaction with TAP42. 1 Publication
Mutagenesisi40 – 401N → A: Normal interaction with TAP42. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Serine/threonine-protein phosphatase PP1-1PRO_0000058881Add
BLAST

Proteomic databases

MaxQBiP20604.
PaxDbiP20604.
PeptideAtlasiP20604.
PRIDEiP20604.

Expressioni

Developmental stagei

Functions in the late cell cycle G1 phase for progression into the S phase, possibly associated in two separate complexes with the phosphorylated forms of p155 and p190, two high MW proteins.

Gene expression databases

GenevestigatoriP20604.

Interactioni

Subunit structurei

Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Forms a ternary complex with RRD1-TAP42.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RRD1P404544EBI-13707,EBI-25278
SAP155P436128EBI-13707,EBI-16370
SAP185P408568EBI-13707,EBI-16384
SAP190P361234EBI-13707,EBI-16392
TAP42Q043726EBI-13707,EBI-18926

Protein-protein interaction databases

BioGridi32011. 169 interactions.
DIPiDIP-5850N.
IntActiP20604. 40 interactions.
MINTiMINT-615434.
STRINGi4932.YDL047W.

Structurei

3D structure databases

ProteinModelPortaliP20604.
SMRiP20604. Positions 7-291.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00550000074961.
HOGENOMiHOG000172696.
KOiK15427.
OMAiQKDSAYT.
OrthoDBiEOG7FFN29.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20604-1 [UniParc]FASTAAdd to Basket

« Hide

MVSRGPDEWL ETIKKCQALT ENEMKQLCEM VKELLMEESN IQPVQTPVTV    50
CGDIHGQFHD LLELFRTAGG FPDDINYIFL GDYVDRGYYS LETFTLLMCL 100
KVKYPAKITL VRGNHESRQI TQVYGFYEEC LNKYGSTTVW KYCCQVFDFL 150
TLAAIIDGKI LCVHGGLSPE IRMLDQIRVL SRAQEVPHEG GFSDLLWSDP 200
DNVEAWQVSP RGAGWLFGSK VAREFNHVNG LNLIARAHQL VMEGFKYHFP 250
EKDVVTVWSA PNYCYRCGNV ASVMKVDEDL EPTFKIFSAV PDDYIRESTA 300
NHNNQRAGYF L 311
Length:311
Mass (Da):35,538
Last modified:February 1, 1991 - v1
Checksum:iAF52BC65E4E715EC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M24395 Genomic DNA. Translation: AAA56864.1.
Z71781 Genomic DNA. Translation: CAA96442.1.
Z74095 Genomic DNA. Translation: CAA98609.1.
BK006938 Genomic DNA. Translation: DAA11808.1.
PIRiA31874. PABY1.
RefSeqiNP_010236.1. NM_001180106.1.

Genome annotation databases

EnsemblFungiiYDL047W; YDL047W; YDL047W.
GeneIDi851513.
KEGGisce:YDL047W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M24395 Genomic DNA. Translation: AAA56864.1 .
Z71781 Genomic DNA. Translation: CAA96442.1 .
Z74095 Genomic DNA. Translation: CAA98609.1 .
BK006938 Genomic DNA. Translation: DAA11808.1 .
PIRi A31874. PABY1.
RefSeqi NP_010236.1. NM_001180106.1.

3D structure databases

ProteinModelPortali P20604.
SMRi P20604. Positions 7-291.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32011. 169 interactions.
DIPi DIP-5850N.
IntActi P20604. 40 interactions.
MINTi MINT-615434.
STRINGi 4932.YDL047W.

Proteomic databases

MaxQBi P20604.
PaxDbi P20604.
PeptideAtlasi P20604.
PRIDEi P20604.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL047W ; YDL047W ; YDL047W .
GeneIDi 851513.
KEGGi sce:YDL047W.

Organism-specific databases

CYGDi YDL047w.
SGDi S000002205. SIT4.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00550000074961.
HOGENOMi HOG000172696.
KOi K15427.
OMAi QKDSAYT.
OrthoDBi EOG7FFN29.

Enzyme and pathway databases

BioCyci YEAST:G3O-29466-MONOMER.

Miscellaneous databases

NextBioi 968878.
PROi P20604.

Gene expression databases

Genevestigatori P20604.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatases."
    Arndt K.T., Styles C.A., Fink G.R.
    Cell 56:527-537(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The SIT4 protein phosphatase functions in late G1 for progression into S phase."
    Sutton A., Immanuel D., Arndt K.T.
    Mol. Cell. Biol. 11:2133-2148(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases."
    Wang H., Wang X., Jiang Y.
    Mol. Biol. Cell 14:4342-4351(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TAP42, MUTAGENESIS OF LEU-35; 37-GLU-GLU-38; GLU-38 AND ASN-40.
  6. "Multiple roles of Tap42 in mediating rapamycin-induced transcriptional changes in yeast."
    Duevel K., Santhanam A., Garrett S., Schneper L., Broach J.R.
    Mol. Cell 11:1467-1478(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TAP42.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2."
    Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J.
    Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPE1 AND RRD1.
  9. "The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes."
    Zheng Y., Jiang Y.
    Mol. Biol. Cell 16:2119-2127(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RRD1 AND TAP42.

Entry informationi

Entry nameiPP11_YEAST
AccessioniPrimary (citable) accession number: P20604
Secondary accession number(s): D6VRU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 3, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3970 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi