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Protein

Guanylate cyclase soluble subunit beta-1

Gene

Gucy1b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP.By similarity

Miscellaneous

There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.By similarity

Cofactori

hemeBy similarityNote: Binds 1 or 2 heme groups per heterodimer. Heme is required for responding to nitric oxide, but not for catalytic activity.By similarity

Enzyme regulationi

Activated by nitric oxide in the presence of magnesium or manganese ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi105Iron (heme proximal ligand)By similarity1

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: RGD
  • heme binding Source: RGD
  • Hsp90 protein binding Source: RGD
  • ion binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • protein heterodimerization activity Source: RGD

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processcGMP biosynthesis
LigandGTP-binding, Heme, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.2 5301

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate cyclase soluble subunit beta-1 (EC:4.6.1.2By similarity)
Short name:
GCS-beta-1
Alternative name(s):
Guanylate cyclase soluble subunit beta-3
Short name:
GCS-beta-3
Soluble guanylate cyclase small subunit
Gene namesi
Name:Gucy1b1
Synonyms:Guc1b3, Gucy1b3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2769 Gucy1b3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi1290

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741181 – 619Guanylate cyclase soluble subunit beta-1Add BLAST619

Proteomic databases

PaxDbiP20595
PRIDEiP20595

PTM databases

iPTMnetiP20595

Expressioni

Tissue specificityi

Lung and brain.

Gene expression databases

BgeeiENSRNOG00000012060
GenevisibleiP20595 RN

Interactioni

Subunit structurei

The active enzyme is formed by a heterodimer of an alpha and a beta subunit. Homotetramer; dimer of dimers (in vitro) (PubMed:20105301). Heterodimer with GUCY1A1. Can also form inactive homodimers in vitro (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg4P310162EBI-7980539,EBI-375655

GO - Molecular functioni

  • Hsp90 protein binding Source: RGD
  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

IntActiP20595, 3 interactors
MINTiP20595
STRINGi10116.ENSRNOP00000062785

Structurei

Secondary structure

1619
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi349 – 396Combined sources48
Helixi400 – 407Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HLSX-ray2.15A/B/C/D/E/F/G/H348-409[»]
ProteinModelPortaliP20595
SMRiP20595
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20595

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini421 – 554Guanylate cyclasePROSITE-ProRule annotationAdd BLAST134

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4171 Eukaryota
COG2114 LUCA
GeneTreeiENSGT00760000118959
HOGENOMiHOG000220903
HOVERGENiHBG051715
InParanoidiP20595
KOiK12319
OMAiEFLCEAP
OrthoDBiEOG091G07GF
PhylomeDBiP20595
TreeFamiTF351403

Family and domain databases

Gene3Di3.30.70.1230, 1 hit
3.90.1520.10, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR018297 A/G_cyclase_CS
IPR038158 H-NOX_domain_sf
IPR011644 Heme_NO-bd
IPR011645 HNOB_dom_associated
IPR024096 NO_sig/Golgi_transp_ligand-bd
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF00211 Guanylate_cyc, 1 hit
PF07700 HNOB, 1 hit
PF07701 HNOBA, 1 hit
SMARTiView protein in SMART
SM00044 CYCc, 1 hit
SUPFAMiSSF111126 SSF111126, 1 hit
SSF55073 SSF55073, 1 hit
PROSITEiView protein in PROSITE
PS00452 GUANYLATE_CYCLASE_1, 1 hit
PS50125 GUANYLATE_CYCLASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P20595-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYGFVNHALE LLVIRNYGPE VWEDIKKEAQ LDEEGQFLVR IIYDDSKTYD
60 70 80 90 100
LVAAASKVLN LNAGEILQMF GKMFFVFCQE SGYDTILRVL GSNVREFLQN
110 120 130 140 150
LDALHDHLAT IYPGMRAPSF RCTDAEKGKG LILHYYSERE GLQDIVIGII
160 170 180 190 200
KTVAQQIHGT EIDMKVIQQR SEECDHTQFL IEEKESKEED FYEDLDRFEE
210 220 230 240 250
NGTQDSRISP YTFCKAFPFH IIFDRDLVVT QCGNAIYRVL PQLQPGKCSL
260 270 280 290 300
LSVFSLVRPH IDISFHGILS HINTVFVLRS KEGLLDVEKL ECEDELTGAE
310 320 330 340 350
ISCLRLKGQM IYLPEADSIL FLCSPSVMNL DDLTRRGLYL SDIPLHDATR
360 370 380 390 400
DLVLLGEQFR EEYKLTQELE ILTDRLQLTL RALEDEKKKT DTLLYSVLPP
410 420 430 440 450
SVANELRHKR PVPAKRYDNV TILFSGIVGF NAFCSKHASG EGAMKIVNLL
460 470 480 490 500
NDLYTRFDTL TDSRKNPFVY KVETVGDKYM TVSGLPEPCI HHARSICHLA
510 520 530 540 550
LDMMEIAGQV QVDGESVQIT IGIHTGEVVT GVIGQRMPRY CLFGNTVNLT
560 570 580 590 600
SRTETTGEKG KINVSEYTYR CLMSPENSDP QFHLEHRGPV SMKGKKEPMQ
610
VWFLSRKNTG TEETNQDEN
Length:619
Mass (Da):70,456
Last modified:January 24, 2006 - v2
Checksum:i9F4B49E341975184
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33E → Q in AAA41204 (PubMed:2905128).Curated1
Sequence conflicti45D → H in AAA41204 (PubMed:2905128).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22562 mRNA Translation: AAA41204.1
AB098025 mRNA Translation: BAC44989.1
BC081840 mRNA Translation: AAH81840.1
PIRiA31871 OYRTB1
RefSeqiNP_036901.2, NM_012769.2
UniGeneiRn.87228

Genome annotation databases

EnsembliENSRNOT00000064930; ENSRNOP00000062785; ENSRNOG00000012060
GeneIDi25202
KEGGirno:25202
UCSCiRGD:2769 rat

Similar proteinsi

Entry informationi

Entry nameiGCYB1_RAT
AccessioniPrimary (citable) accession number: P20595
Secondary accession number(s): Q8CH88
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 24, 2006
Last modified: April 25, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health