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P20592 (MX2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon-induced GTP-binding protein Mx2
Alternative name(s):
Interferon-regulated resistance GTP-binding protein MxB
Myxovirus resistance protein 2
p78-related protein
Gene names
Name:MX2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length715 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interferon-induced dynamin-like GTPase with potent antiviral activity against human immunodeficiency virus type 1 (HIV-1). Acts by targeting the viral capsid and affects the nuclear uptake and/or stability of the HIV-1 replication complex and the subsequent chromosomal integration of the proviral DNA. Exhibits antiviral activity also against simian immunodeficiency virus (SIV-mnd). May play a role in regulating nucleocytoplasmic transport and cell-cycle progression. Ref.6 Ref.10 Ref.11 Ref.12

Subcellular location

Cytoplasm. Nucleus. Nucleusnuclear pore complex. Note: Localization to nuclear pores requires GTP-binding. Ref.6

Induction

By type I and type III interferons. Ref.7

Sequence similarities

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.

Contains 1 dynamin-type G (guanine nucleotide-binding) domain.

Contains 1 GED domain.

Ontologies

Keywords
   Biological processAntiviral defense
Immunity
Innate immunity
mRNA transport
Protein transport
Translocation
Transport
   Cellular componentCytoplasm
Nuclear pore complex
Nucleus
   LigandGTP-binding
Nucleotide-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Traceable author statement PubMed 8798556. Source: GOC

cytokine-mediated signaling pathway

Traceable author statement. Source: Reactome

defense response

Traceable author statement PubMed 8798556. Source: ProtInc

defense response to virus

Inferred from mutant phenotype Ref.12. Source: UniProtKB

mRNA transport

Inferred from electronic annotation. Source: UniProtKB-KW

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of cell cycle

Inferred from mutant phenotype Ref.6. Source: UniProtKB

regulation of nucleocytoplasmic transport

Inferred from mutant phenotype Ref.6. Source: UniProtKB

response to interferon-alpha

Inferred from direct assay Ref.12. Source: UniProtKB

response to virus

Inferred from direct assay Ref.11. Source: UniProtKB

type I interferon signaling pathway

Traceable author statement. Source: Reactome

   Cellular_componentcytoplasm

Traceable author statement PubMed 8798556. Source: ProtInc

cytosol

Traceable author statement. Source: Reactome

nuclear pore

Inferred from direct assay Ref.6. Source: UniProtKB

nucleus

Traceable author statement PubMed 8798556. Source: ProtInc

   Molecular_functionGTP binding

Inferred from mutant phenotype Ref.6. Source: UniProtKB

GTPase activity

Traceable author statement PubMed 8798556. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 715715Interferon-induced GTP-binding protein Mx2
PRO_0000206598

Regions

Domain115 – 387273Dynamin-type G
Domain623 – 71492GED
Nucleotide binding125 – 1328GTP Potential
Nucleotide binding225 – 2295GTP Potential
Nucleotide binding294 – 2974GTP Potential

Experimental info

Mutagenesis1311K → A: Loss of GTP-binding and localization to nuclear pore. Disruption of nuclear import. Ref.6
Mutagenesis1511T → A: Defective GTP-hydrolysis. Disruption of nuclear import and cell-cycle progression. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P20592 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 1AE4B80157545344

FASTA71582,089
        10         20         30         40         50         60 
MSKAHKPWPY RRRSQFSSRK YLKKEMNSFQ QQPPPFGTVP PQMMFPPNWQ GAEKDAAFLA 

        70         80         90        100        110        120 
KDFNFLTLNN QPPPGNRSQP RAMGPENNLY SQYEQKVRPC IDLIDSLRAL GVEQDLALPA 

       130        140        150        160        170        180 
IAVIGDQSSG KSSVLEALSG VALPRGSGIV TRCPLVLKLK KQPCEAWAGR ISYRNTELEL 

       190        200        210        220        230        240 
QDPGQVEKEI HKAQNVMAGN GRGISHELIS LEITSPEVPD LTIIDLPGIT RVAVDNQPRD 

       250        260        270        280        290        300 
IGLQIKALIK KYIQRQQTIN LVVVPCNVDI ATTEALSMAH EVDPEGDRTI GILTKPDLMD 

       310        320        330        340        350        360 
RGTEKSVMNV VRNLTYPLKK GYMIVKCRGQ QEITNRLSLA EATKKEITFF QTHPYFRVLL 

       370        380        390        400        410        420 
EEGSATVPRL AERLTTELIM HIQKSLPLLE GQIRESHQKA TEELRRCGAD IPSQEADKMF 

       430        440        450        460        470        480 
FLIEKIKMFN QDIEKLVEGE EVVRENETRL YNKIREDFKN WVGILATNTQ KVKNIIHEEV 

       490        500        510        520        530        540 
EKYEKQYRGK ELLGFVNYKT FEIIVHQYIQ QLVEPALSML QKAMEIIQQA FINVAKKHFG 

       550        560        570        580        590        600 
EFFNLNQTVQ STIEDIKVKH TAKAENMIQL QFRMEQMVFC QDQIYSVVLK KVREEIFNPL 

       610        620        630        640        650        660 
GTPSQNMKLN SHFPSNESSV SSFTEIGIHL NAYFLETSKR LANQIPFIIQ YFMLRENGDS 

       670        680        690        700        710 
LQKAMMQILQ EKNRYSWLLQ EQSETATKRR ILKERIYRLT QARHALCQFS SKEIH 

« Hide

References

« Hide 'large scale' references
[1]"cDNA structures and regulation of two interferon-induced human Mx proteins."
Aebi M., Faeh J., Hurt N., Samuel C.E., Thomis D., Bazzigher L., Pavlovic J., Haller O., Staeheli P.
Mol. Cell. Biol. 9:5062-5072(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The DNA sequence of human chromosome 21."
Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. expand/collapse author list , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"Cloning and sequence analyses of cDNAs for interferon- and virus-induced human Mx proteins reveal that they contain putative guanine nucleotide-binding sites: functional study of the corresponding gene promoter."
Horisberger M.A., McMaster G.K., Zeller H., Wathelet M.G., Dellis J., Content J.
J. Virol. 64:1171-1181(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-715.
[6]"Inhibition of nuclear import and cell-cycle progression by mutated forms of the dynamin-like GTPase MxB."
King M.C., Raposo G., Lemmon M.A.
Proc. Natl. Acad. Sci. U.S.A. 101:8957-8962(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-131 AND THR-151.
[7]"The Mx GTPase family of interferon-induced antiviral proteins."
Haller O., Stertz S., Kochs G.
Microbes Infect. 9:1636-1643(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW, INDUCTION.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Dynamins are forever: MxB inhibits HIV-1."
Haller O.
Cell Host Microbe 14:371-373(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[10]"The interferon-inducible MxB protein inhibits HIV-1 infection."
Liu Z., Pan Q., Ding S., Qian J., Xu F., Zhou J., Cen S., Guo F., Liang C.
Cell Host Microbe 14:398-410(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN HIV-1 RESTRICTION.
[11]"Human MX2 is an interferon-induced post-entry inhibitor of HIV-1 infection."
Goujon C., Moncorge O., Bauby H., Doyle T., Ward C.C., Schaller T., Hue S., Barclay W.S., Schulz R., Malim M.H.
Nature 502:559-562(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN HIV-1 RESTRICTION.
[12]"MX2 is an interferon-induced inhibitor of HIV-1 infection."
Kane M., Yadav S.S., Bitzegeio J., Kutluay S.B., Zang T., Wilson S.J., Schoggins J.W., Rice C.M., Yamashita M., Hatziioannou T., Bieniasz P.D.
Nature 502:563-566(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN HIV-1 RESTRICTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M30818 mRNA. Translation: AAA36338.1.
M33883 mRNA. Translation: AAA36459.1.
AL163285 Genomic DNA. Translation: CAB90555.1.
CH471079 Genomic DNA. Translation: EAX09605.1.
CH471079 Genomic DNA. Translation: EAX09606.1.
BC035293 mRNA. Translation: AAH35293.1.
CCDSCCDS13672.1.
PIRB33481.
RefSeqNP_002454.1. NM_002463.1.
XP_005261040.1. XM_005260983.2.
XP_005261041.1. XM_005260984.1.
UniGeneHs.926.

3D structure databases

ProteinModelPortalP20592.
SMRP20592. Positions 93-709.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110685. 2 interactions.
DIPDIP-59212N.
STRING9606.ENSP00000333657.

PTM databases

PhosphoSiteP20592.

Polymorphism databases

DMDM127571.

Proteomic databases

MaxQBP20592.
PaxDbP20592.
PRIDEP20592.

Protocols and materials databases

DNASU4600.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000330714; ENSP00000333657; ENSG00000183486.
GeneID4600.
KEGGhsa:4600.
UCSCuc002yzf.1. human.

Organism-specific databases

CTD4600.
GeneCardsGC21P042734.
HGNCHGNC:7533. MX2.
HPAHPA030235.
MIM147890. gene.
neXtProtNX_P20592.
PharmGKBPA31334.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0699.
HOGENOMHOG000063238.
HOVERGENHBG008788.
InParanoidP20592.
OMANTQKVKN.
OrthoDBEOG7034GF.
PhylomeDBP20592.
TreeFamTF331484.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressP20592.
BgeeP20592.
CleanExHS_MX2.
GenevestigatorP20592.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR003130. GED.
IPR020850. GTPase_effector_domain_GED.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR11566. PTHR11566. 1 hit.
PfamPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSPR00195. DYNAMIN.
SMARTSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMX2. human.
GeneWikiMX2.
GenomeRNAi4600.
NextBio17688.
PROP20592.
SOURCESearch...

Entry information

Entry nameMX2_HUMAN
AccessionPrimary (citable) accession number: P20592
Secondary accession number(s): D3DSI7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 21

Human chromosome 21: entries, gene names and cross-references to MIM