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P20592

- MX2_HUMAN

UniProt

P20592 - MX2_HUMAN

Protein

Interferon-induced GTP-binding protein Mx2

Gene

MX2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Interferon-induced dynamin-like GTPase with potent antiviral activity against human immunodeficiency virus type 1 (HIV-1). Acts by targeting the viral capsid and affects the nuclear uptake and/or stability of the HIV-1 replication complex and the subsequent chromosomal integration of the proviral DNA. Exhibits antiviral activity also against simian immunodeficiency virus (SIV-mnd). May play a role in regulating nucleocytoplasmic transport and cell-cycle progression.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi125 – 1328GTPSequence Analysis
    Nucleotide bindingi225 – 2295GTPSequence Analysis
    Nucleotide bindingi294 – 2974GTPSequence Analysis

    GO - Molecular functioni

    1. GTPase activity Source: ProtInc
    2. GTP binding Source: UniProtKB

    GO - Biological processi

    1. cytokine-mediated signaling pathway Source: Reactome
    2. defense response Source: ProtInc
    3. defense response to virus Source: UniProtKB
    4. GTP catabolic process Source: GOC
    5. mRNA transport Source: UniProtKB-KW
    6. protein transport Source: UniProtKB-KW
    7. regulation of cell cycle Source: UniProtKB
    8. regulation of nucleocytoplasmic transport Source: UniProtKB
    9. response to interferon-alpha Source: UniProtKB
    10. response to virus Source: UniProtKB
    11. type I interferon signaling pathway Source: Reactome

    Keywords - Biological processi

    Antiviral defense, Immunity, Innate immunity, mRNA transport, Protein transport, Translocation, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_115831. ISG15 antiviral mechanism.
    REACT_25162. Interferon alpha/beta signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interferon-induced GTP-binding protein Mx2
    Alternative name(s):
    Interferon-regulated resistance GTP-binding protein MxB
    Myxovirus resistance protein 2
    p78-related protein
    Gene namesi
    Name:MX2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 21

    Organism-specific databases

    HGNCiHGNC:7533. MX2.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication. Nucleusnuclear pore complex 1 Publication
    Note: Localization to nuclear pores requires GTP-binding.

    GO - Cellular componenti

    1. cytoplasm Source: ProtInc
    2. cytosol Source: Reactome
    3. nuclear pore Source: UniProtKB
    4. nucleus Source: ProtInc

    Keywords - Cellular componenti

    Cytoplasm, Nuclear pore complex, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi131 – 1311K → A: Loss of GTP-binding and localization to nuclear pore. Disruption of nuclear import. 1 Publication
    Mutagenesisi151 – 1511T → A: Defective GTP-hydrolysis. Disruption of nuclear import and cell-cycle progression. 1 Publication

    Organism-specific databases

    PharmGKBiPA31334.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 715715Interferon-induced GTP-binding protein Mx2PRO_0000206598Add
    BLAST

    Proteomic databases

    MaxQBiP20592.
    PaxDbiP20592.
    PRIDEiP20592.

    PTM databases

    PhosphoSiteiP20592.

    Expressioni

    Inductioni

    By type I and type III interferons.1 Publication

    Gene expression databases

    ArrayExpressiP20592.
    BgeeiP20592.
    CleanExiHS_MX2.
    GenevestigatoriP20592.

    Organism-specific databases

    HPAiHPA030235.

    Interactioni

    Protein-protein interaction databases

    BioGridi110685. 2 interactions.
    DIPiDIP-59212N.
    STRINGi9606.ENSP00000333657.

    Structurei

    3D structure databases

    ProteinModelPortaliP20592.
    SMRiP20592. Positions 93-709.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini115 – 387273Dynamin-type GAdd
    BLAST
    Domaini623 – 71492GEDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 GED domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0699.
    HOGENOMiHOG000063238.
    HOVERGENiHBG008788.
    InParanoidiP20592.
    OMAiNTQKVKN.
    OrthoDBiEOG7034GF.
    PhylomeDBiP20592.
    TreeFamiTF331484.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR000375. Dynamin_central.
    IPR001401. Dynamin_GTPase.
    IPR019762. Dynamin_GTPase_CS.
    IPR022812. Dynamin_SF.
    IPR003130. GED.
    IPR020850. GTPase_effector_domain_GED.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11566. PTHR11566. 1 hit.
    PfamiPF01031. Dynamin_M. 1 hit.
    PF00350. Dynamin_N. 1 hit.
    PF02212. GED. 1 hit.
    [Graphical view]
    PRINTSiPR00195. DYNAMIN.
    SMARTiSM00053. DYNc. 1 hit.
    SM00302. GED. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    PS51388. GED. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P20592-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSKAHKPWPY RRRSQFSSRK YLKKEMNSFQ QQPPPFGTVP PQMMFPPNWQ    50
    GAEKDAAFLA KDFNFLTLNN QPPPGNRSQP RAMGPENNLY SQYEQKVRPC 100
    IDLIDSLRAL GVEQDLALPA IAVIGDQSSG KSSVLEALSG VALPRGSGIV 150
    TRCPLVLKLK KQPCEAWAGR ISYRNTELEL QDPGQVEKEI HKAQNVMAGN 200
    GRGISHELIS LEITSPEVPD LTIIDLPGIT RVAVDNQPRD IGLQIKALIK 250
    KYIQRQQTIN LVVVPCNVDI ATTEALSMAH EVDPEGDRTI GILTKPDLMD 300
    RGTEKSVMNV VRNLTYPLKK GYMIVKCRGQ QEITNRLSLA EATKKEITFF 350
    QTHPYFRVLL EEGSATVPRL AERLTTELIM HIQKSLPLLE GQIRESHQKA 400
    TEELRRCGAD IPSQEADKMF FLIEKIKMFN QDIEKLVEGE EVVRENETRL 450
    YNKIREDFKN WVGILATNTQ KVKNIIHEEV EKYEKQYRGK ELLGFVNYKT 500
    FEIIVHQYIQ QLVEPALSML QKAMEIIQQA FINVAKKHFG EFFNLNQTVQ 550
    STIEDIKVKH TAKAENMIQL QFRMEQMVFC QDQIYSVVLK KVREEIFNPL 600
    GTPSQNMKLN SHFPSNESSV SSFTEIGIHL NAYFLETSKR LANQIPFIIQ 650
    YFMLRENGDS LQKAMMQILQ EKNRYSWLLQ EQSETATKRR ILKERIYRLT 700
    QARHALCQFS SKEIH 715
    Length:715
    Mass (Da):82,089
    Last modified:February 1, 1991 - v1
    Checksum:i1AE4B80157545344
    GO
    Isoform 2 (identifier: P20592-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         148-192: Missing.
         245-246: IK → VS
         247-715: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:201
    Mass (Da):22,105
    Checksum:i393996B23F611D6E
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei148 – 19245Missing in isoform 2. 1 PublicationVSP_056443Add
    BLAST
    Alternative sequencei245 – 2462IK → VS in isoform 2. 1 PublicationVSP_056444
    Alternative sequencei247 – 715469Missing in isoform 2. 1 PublicationVSP_056445Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30818 mRNA. Translation: AAA36338.1.
    M33883 mRNA. Translation: AAA36459.1.
    AK298780 mRNA. Translation: BAH12869.1.
    AL163285 Genomic DNA. Translation: CAB90555.1.
    AL773578 Genomic DNA. No translation available.
    CH471079 Genomic DNA. Translation: EAX09605.1.
    CH471079 Genomic DNA. Translation: EAX09606.1.
    BC035293 mRNA. Translation: AAH35293.1.
    CCDSiCCDS13672.1.
    PIRiB33481.
    RefSeqiNP_002454.1. NM_002463.1.
    XP_005261040.1. XM_005260983.2.
    XP_005261041.1. XM_005260984.1.
    UniGeneiHs.926.

    Genome annotation databases

    EnsembliENST00000330714; ENSP00000333657; ENSG00000183486.
    ENST00000543692; ENSP00000446017; ENSG00000183486.
    GeneIDi4600.
    KEGGihsa:4600.
    UCSCiuc002yzf.1. human.

    Polymorphism databases

    DMDMi127571.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30818 mRNA. Translation: AAA36338.1 .
    M33883 mRNA. Translation: AAA36459.1 .
    AK298780 mRNA. Translation: BAH12869.1 .
    AL163285 Genomic DNA. Translation: CAB90555.1 .
    AL773578 Genomic DNA. No translation available.
    CH471079 Genomic DNA. Translation: EAX09605.1 .
    CH471079 Genomic DNA. Translation: EAX09606.1 .
    BC035293 mRNA. Translation: AAH35293.1 .
    CCDSi CCDS13672.1.
    PIRi B33481.
    RefSeqi NP_002454.1. NM_002463.1.
    XP_005261040.1. XM_005260983.2.
    XP_005261041.1. XM_005260984.1.
    UniGenei Hs.926.

    3D structure databases

    ProteinModelPortali P20592.
    SMRi P20592. Positions 93-709.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110685. 2 interactions.
    DIPi DIP-59212N.
    STRINGi 9606.ENSP00000333657.

    PTM databases

    PhosphoSitei P20592.

    Polymorphism databases

    DMDMi 127571.

    Proteomic databases

    MaxQBi P20592.
    PaxDbi P20592.
    PRIDEi P20592.

    Protocols and materials databases

    DNASUi 4600.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000330714 ; ENSP00000333657 ; ENSG00000183486 .
    ENST00000543692 ; ENSP00000446017 ; ENSG00000183486 .
    GeneIDi 4600.
    KEGGi hsa:4600.
    UCSCi uc002yzf.1. human.

    Organism-specific databases

    CTDi 4600.
    GeneCardsi GC21P042734.
    HGNCi HGNC:7533. MX2.
    HPAi HPA030235.
    MIMi 147890. gene.
    neXtProti NX_P20592.
    PharmGKBi PA31334.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0699.
    HOGENOMi HOG000063238.
    HOVERGENi HBG008788.
    InParanoidi P20592.
    OMAi NTQKVKN.
    OrthoDBi EOG7034GF.
    PhylomeDBi P20592.
    TreeFami TF331484.

    Enzyme and pathway databases

    Reactomei REACT_115831. ISG15 antiviral mechanism.
    REACT_25162. Interferon alpha/beta signaling.

    Miscellaneous databases

    ChiTaRSi MX2. human.
    GeneWikii MX2.
    GenomeRNAii 4600.
    NextBioi 17688.
    PROi P20592.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P20592.
    Bgeei P20592.
    CleanExi HS_MX2.
    Genevestigatori P20592.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR000375. Dynamin_central.
    IPR001401. Dynamin_GTPase.
    IPR019762. Dynamin_GTPase_CS.
    IPR022812. Dynamin_SF.
    IPR003130. GED.
    IPR020850. GTPase_effector_domain_GED.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11566. PTHR11566. 1 hit.
    Pfami PF01031. Dynamin_M. 1 hit.
    PF00350. Dynamin_N. 1 hit.
    PF02212. GED. 1 hit.
    [Graphical view ]
    PRINTSi PR00195. DYNAMIN.
    SMARTi SM00053. DYNc. 1 hit.
    SM00302. GED. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00410. G_DYNAMIN_1. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    PS51388. GED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA structures and regulation of two interferon-induced human Mx proteins."
      Aebi M., Faeh J., Hurt N., Samuel C.E., Thomis D., Bazzigher L., Pavlovic J., Haller O., Staeheli P.
      Mol. Cell. Biol. 9:5062-5072(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    3. "The DNA sequence of human chromosome 21."
      Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A.
      , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
      Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    6. "Cloning and sequence analyses of cDNAs for interferon- and virus-induced human Mx proteins reveal that they contain putative guanine nucleotide-binding sites: functional study of the corresponding gene promoter."
      Horisberger M.A., McMaster G.K., Zeller H., Wathelet M.G., Dellis J., Content J.
      J. Virol. 64:1171-1181(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-715 (ISOFORM 1).
    7. "Inhibition of nuclear import and cell-cycle progression by mutated forms of the dynamin-like GTPase MxB."
      King M.C., Raposo G., Lemmon M.A.
      Proc. Natl. Acad. Sci. U.S.A. 101:8957-8962(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-131 AND THR-151.
    8. "The Mx GTPase family of interferon-induced antiviral proteins."
      Haller O., Stertz S., Kochs G.
      Microbes Infect. 9:1636-1643(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW, INDUCTION.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Dynamins are forever: MxB inhibits HIV-1."
      Haller O.
      Cell Host Microbe 14:371-373(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    11. "The interferon-inducible MxB protein inhibits HIV-1 infection."
      Liu Z., Pan Q., Ding S., Qian J., Xu F., Zhou J., Cen S., Guo F., Liang C.
      Cell Host Microbe 14:398-410(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN HIV-1 RESTRICTION.
    12. "Human MX2 is an interferon-induced post-entry inhibitor of HIV-1 infection."
      Goujon C., Moncorge O., Bauby H., Doyle T., Ward C.C., Schaller T., Hue S., Barclay W.S., Schulz R., Malim M.H.
      Nature 502:559-562(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN HIV-1 RESTRICTION.
    13. Cited for: FUNCTION IN HIV-1 RESTRICTION.

    Entry informationi

    Entry nameiMX2_HUMAN
    AccessioniPrimary (citable) accession number: P20592
    Secondary accession number(s): B7Z5D3, D3DSI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 21
      Human chromosome 21: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3