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P20590

- MTH1_HAEIF

UniProt

P20590 - MTH1_HAEIF

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Protein

Modification methylase HinfI

Gene

hinfIM

Organism
Haemophilus influenzae
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HinfI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3429. M.HinfI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HinfI (EC:2.1.1.72)
Short name:
M.HinfI
Alternative name(s):
Adenine-specific methyltransferase HinfI
Gene namesi
Name:hinfIM
OrganismiHaemophilus influenzae
Taxonomic identifieri727 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Modification methylase HinfIPRO_0000087963Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP20590.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20590-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMKENINDFL NTILKGDCIE KLKTIPNESI DLIFADPPYF MQTEGKLLRT
60 70 80 90 100
NGDEFSGVDD EWDKFNDFVE YDSFCELWLK ECKRILKSTG SIWVIGSFQN
110 120 130 140 150
IYRIGYIMQN LDFWILNDVI WNKTNPVPNF GGTRFCNAHE TMLWCSKCKK
160 170 180 190 200
NKFTFNYKTM KHLNQEKQER SVWSLSLCTG KERIKDEEGK KAHSTQKPES
210 220 230 240 250
LLYKVILSSS KPNDVVLDPF FGTGTTGAVA KALGRNYIGI EREQKYIDVA
260 270 280 290 300
EKRLREIKPN PNDIELLSLE IKPPKVPMKT LIEADFLRVG QTLFDKNENA
310 320 330 340 350
ICIVTQDGNV KDNEETLSIH KMSAKYLNKT NNNGWDYFYL FRNNNFITLD

SLRYEYTNQ
Length:359
Mass (Da):41,806
Last modified:February 1, 1991 - v1
Checksum:i128C6BB0D5480621
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M22862 Genomic DNA. Translation: AAA24986.1.
PIRiJT0391.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M22862 Genomic DNA. Translation: AAA24986.1 .
PIRi JT0391.

3D structure databases

ProteinModelPortali P20590.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3429. M.HinfI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00508. S21N4MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing the HinfI restriction and modification genes."
    Chandrasegaran S., Lunnen K.D., Smith H.O., Wilson G.G.
    Gene 70:387-392(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RF.

Entry informationi

Entry nameiMTH1_HAEIF
AccessioniPrimary (citable) accession number: P20590
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: October 1, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3