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P20590 (MTH1_HAEIF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HinfI

Short name=M.HinfI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase HinfI
Gene names
Name:hinfIM
OrganismHaemophilus influenzae
Taxonomic identifier727 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HinfI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359Modification methylase HinfI
PRO_0000087963

Sequences

Sequence LengthMass (Da)Tools
P20590 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 128C6BB0D5480621

FASTA35941,806
        10         20         30         40         50         60 
MMKENINDFL NTILKGDCIE KLKTIPNESI DLIFADPPYF MQTEGKLLRT NGDEFSGVDD 

        70         80         90        100        110        120 
EWDKFNDFVE YDSFCELWLK ECKRILKSTG SIWVIGSFQN IYRIGYIMQN LDFWILNDVI 

       130        140        150        160        170        180 
WNKTNPVPNF GGTRFCNAHE TMLWCSKCKK NKFTFNYKTM KHLNQEKQER SVWSLSLCTG 

       190        200        210        220        230        240 
KERIKDEEGK KAHSTQKPES LLYKVILSSS KPNDVVLDPF FGTGTTGAVA KALGRNYIGI 

       250        260        270        280        290        300 
EREQKYIDVA EKRLREIKPN PNDIELLSLE IKPPKVPMKT LIEADFLRVG QTLFDKNENA 

       310        320        330        340        350 
ICIVTQDGNV KDNEETLSIH KMSAKYLNKT NNNGWDYFYL FRNNNFITLD SLRYEYTNQ 

« Hide

References

[1]"Cloning and sequencing the HinfI restriction and modification genes."
Chandrasegaran S., Lunnen K.D., Smith H.O., Wilson G.G.
Gene 70:387-392(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: RF.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M22862 Genomic DNA. Translation: AAA24986.1.
PIRJT0391.

3D structure databases

ProteinModelPortalP20590.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3429. M.HinfI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSPR00508. S21N4MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTH1_HAEIF
AccessionPrimary (citable) accession number: P20590
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries