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P20590

- MTH1_HAEIF

UniProt

P20590 - MTH1_HAEIF

Protein

Modification methylase HinfI

Gene

hinfIM

Organism
Haemophilus influenzae
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HinfI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3429. M.HinfI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HinfI (EC:2.1.1.72)
    Short name:
    M.HinfI
    Alternative name(s):
    Adenine-specific methyltransferase HinfI
    Gene namesi
    Name:hinfIM
    OrganismiHaemophilus influenzae
    Taxonomic identifieri727 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 359359Modification methylase HinfIPRO_0000087963Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP20590.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00508. S21N4MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20590-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMKENINDFL NTILKGDCIE KLKTIPNESI DLIFADPPYF MQTEGKLLRT    50
    NGDEFSGVDD EWDKFNDFVE YDSFCELWLK ECKRILKSTG SIWVIGSFQN 100
    IYRIGYIMQN LDFWILNDVI WNKTNPVPNF GGTRFCNAHE TMLWCSKCKK 150
    NKFTFNYKTM KHLNQEKQER SVWSLSLCTG KERIKDEEGK KAHSTQKPES 200
    LLYKVILSSS KPNDVVLDPF FGTGTTGAVA KALGRNYIGI EREQKYIDVA 250
    EKRLREIKPN PNDIELLSLE IKPPKVPMKT LIEADFLRVG QTLFDKNENA 300
    ICIVTQDGNV KDNEETLSIH KMSAKYLNKT NNNGWDYFYL FRNNNFITLD 350
    SLRYEYTNQ 359
    Length:359
    Mass (Da):41,806
    Last modified:February 1, 1991 - v1
    Checksum:i128C6BB0D5480621
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22862 Genomic DNA. Translation: AAA24986.1.
    PIRiJT0391.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22862 Genomic DNA. Translation: AAA24986.1 .
    PIRi JT0391.

    3D structure databases

    ProteinModelPortali P20590.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3429. M.HinfI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00508. S21N4MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing the HinfI restriction and modification genes."
      Chandrasegaran S., Lunnen K.D., Smith H.O., Wilson G.G.
      Gene 70:387-392(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: RF.

    Entry informationi

    Entry nameiMTH1_HAEIF
    AccessioniPrimary (citable) accession number: P20590
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3