Reviewed, UniProtKB/Swiss-Prot P20590 (MTH1_HAEIN)

Names and origin

Protein names

Recommended name:
    Modification methylase HinfI
      Short name=M.HinfI
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase HinfI

Gene names

Name:

hinfIM

Organism

Haemophilus influenzae

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HinfI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Modification methylase HinfI		PRO_0000087963

Sequences

	Sequence		Length	Mass (Da)
	P20590-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 128C6BB0D5480621	FASTA	359	41,806
	10 20 30 40 50 60 MMKENINDFL NTILKGDCIE KLKTIPNESI DLIFADPPYF MQTEGKLLRT NGDEFSGVDD 70 80 90 100 110 120 EWDKFNDFVE YDSFCELWLK ECKRILKSTG SIWVIGSFQN IYRIGYIMQN LDFWILNDVI 130 140 150 160 170 180 WNKTNPVPNF GGTRFCNAHE TMLWCSKCKK NKFTFNYKTM KHLNQEKQER SVWSLSLCTG 190 200 210 220 230 240 KERIKDEEGK KAHSTQKPES LLYKVILSSS KPNDVVLDPF FGTGTTGAVA KALGRNYIGI 250 260 270 280 290 300 EREQKYIDVA EKRLREIKPN PNDIELLSLE IKPPKVPMKT LIEADFLRVG QTLFDKNENA 310 320 330 340 350 ICIVTQDGNV KDNEETLSIH KMSAKYLNKT NNNGWDYFYL FRNNNFITLD SLRYEYTNQ

References

[1]	"Cloning and sequencing the HinfI restriction and modification genes." Chandrasegaran S., Lunnen K.D., Smith H.O., Wilson G.G. Gene 70:387-392(1988) [PubMed: 3063606] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RF.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M22862 Genomic DNA. Translation: AAA24986.1.
PIR	JT0391.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Protein family/group databases
REBASE	3429. M.HinfI.
Enzyme and pathway databases
BRENDA	2.1.1.72. 109.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. MeTrfase_CN4. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTH1_HAEIN

Accession

Primary (citable) accession number: P20590

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	September 22, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)