Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P20589

- MTH3_HAEAE

UniProt

P20589 - MTH3_HAEAE

Protein

Modification methylase HaeIII

Gene

haeIIIM

Organism
Haemophilus aegyptius
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the HaeIII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei71 – 7111 PublicationPROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3410. M.HaeIII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HaeIII (EC:2.1.1.37)
    Short name:
    M.HaeIII
    Alternative name(s):
    Cytosine-specific methyltransferase HaeIII
    Gene namesi
    Name:haeIIIM
    OrganismiHaemophilus aegyptius
    Taxonomic identifieri197575 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 330330Modification methylase HaeIIIPRO_0000087878Add
    BLAST

    Structurei

    Secondary structure

    1
    330
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 76
    Helixi12 – 198
    Beta strandi23 – 297
    Turni32 – 343
    Helixi35 – 417
    Beta strandi44 – 496
    Helixi51 – 533
    Helixi56 – 583
    Beta strandi63 – 664
    Helixi71 – 733
    Beta strandi75 – 784
    Beta strandi83 – 853
    Helixi86 – 883
    Helixi89 – 10113
    Beta strandi104 – 1107
    Helixi113 – 1164
    Helixi117 – 1193
    Helixi121 – 13313
    Beta strandi135 – 1439
    Helixi144 – 1463
    Beta strandi154 – 1629
    Helixi163 – 1653
    Helixi181 – 1833
    Helixi186 – 1883
    Beta strandi189 – 1913
    Helixi197 – 1993
    Helixi203 – 2053
    Beta strandi206 – 2083
    Helixi222 – 2243
    Helixi242 – 2443
    Beta strandi255 – 2584
    Beta strandi261 – 2633
    Turni266 – 2683
    Helixi269 – 2713
    Helixi277 – 2848
    Helixi297 – 3059
    Helixi310 – 32617

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DCTX-ray2.80A/B1-324[»]
    3UBTX-ray2.50A/B/Y1-330[»]
    ProteinModelPortaliP20589.
    SMRiP20589. Positions 1-324.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP20589.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 327327SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20589-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLISLFSGA GGLDLGFQKA GFRIICANEY DKSIWKTYES NHSAKLIKGD    50
    ISKISSDEFP KCDGIIGGPP CQSWSEGGSL RGIDDPRGKL FYEYIRILKQ 100
    KKPIFFLAEN VKGMMAQRHN KAVQEFIQEF DNAGYDVHII LLNANDYGVA 150
    QDRKRVFYIG FRKELNINYL PPIPHLIKPT FKDVIWDLKD NPIPALDKNK 200
    TNGNKCIYPN HEYFIGSYST IFMSRNRVRQ WNEPAFTVQA SGRQCQLHPQ 250
    APVMLKVSKN LNKFVEGKEH LYRRLTVREC ARVQGFPDDF IFHYESLNDG 300
    YKMIGNAVPV NLAYEIAKTI KSALEICKGN 330
    Length:330
    Mass (Da):37,686
    Last modified:February 1, 1991 - v1
    Checksum:i928C6D5390DBED25
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24625 Genomic DNA. Translation: AAA24970.1.
    AF051375 Genomic DNA. Translation: AAC05696.1.
    PIRiJS0102.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24625 Genomic DNA. Translation: AAA24970.1 .
    AF051375 Genomic DNA. Translation: AAC05696.1 .
    PIRi JS0102.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DCT X-ray 2.80 A/B 1-324 [» ]
    3UBT X-ray 2.50 A/B/Y 1-330 [» ]
    ProteinModelPortali P20589.
    SMRi P20589. Positions 1-324.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3410. M.HaeIII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P20589.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and analysis of the HaeIII and HaeII methyltransferase genes."
      Slatko B.E., Croft R., Moran L.S., Wilson G.G.
      Gene 74:45-50(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 11116 / CCUG 25716 / NCTC 8502.
    2. Zhang B.-H., Wilson G.G.
      Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 11116 / CCUG 25716 / NCTC 8502.
    3. "Direct identification of the active-site nucleophile in a DNA (cytosine-5)-methyltransferase."
      Chen L., McMillan A.M., Chang W., Ezak-Nipkay K., Lane W.S., Verdine G.L.
      Biochemistry 30:11018-11025(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVE SITE, PROTEIN SEQUENCE OF 62-81.
    4. "The crystal structure of HaeIII methyltransferase covalently complexed to DNA: an extrahelical cytosine and rearranged base pairing."
      Reinisch K.M., Chen L., Verdine G.L., Lipscomb W.N.
      Cell 82:143-153(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

    Entry informationi

    Entry nameiMTH3_HAEAE
    AccessioniPrimary (citable) accession number: P20589
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3