ID   T2F1_HAEIF              Reviewed;         262 AA.
AC   P20588;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 3.
DT   03-MAY-2023, entry version 72.
DE   RecName: Full=Type II restriction enzyme HinfI {ECO:0000303|PubMed:12654995};
DE            Short=R.HinfI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease HinfI;
DE   AltName: Full=Type-2 restriction enzyme HinfI;
GN   Name=hinfIR;
OS   Haemophilus influenzae.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=727;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=RF;
RX   PubMed=3063606; DOI=10.1016/0378-1119(88)90210-7;
RA   Chandrasegaran S., Lunnen K.D., Smith H.O., Wilson G.G.;
RT   "Cloning and sequencing the HinfI restriction and modification genes.";
RL   Gene 70:387-392(1988).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GANTC-3' and cleaves after G-1.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:3063606}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; M22862; AAA24988.1; -; Genomic_DNA.
DR   PIR; JT0390; JT0390.
DR   RefSeq; WP_021035598.1; NZ_VOFM01000006.1.
DR   AlphaFoldDB; P20588; -.
DR   REBASE; 1154; HinfI.
DR   BRENDA; 3.1.21.4; 2529.
DR   PRO; PR:P20588; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Restriction system.
FT   CHAIN           1..262
FT                   /note="Type II restriction enzyme HinfI"
FT                   /id="PRO_0000077323"
SQ   SEQUENCE   262 AA;  30912 MW;  A65452B593FE8BD1 CRC64;
     MNDEISDLIN QAVSNILINS SSENKLKKLI KTHDVKIHFV PRNYRIFGGI LQSMNIQFGN
     FLEEFMTLLI KSDGRYDILE EYSGKKSNKF QLSTSNDNRI DQFISFCQRS DSINLDEEFP
     KLLNEVKNDN DTNLSSISHD IDILFRNKET GVIYYLEVKY NDDHDTGKFV DINRKFIKTY
     AYLVREFPNT EIKPILFFFN NKKMKGNIYV PENTNIRRGK SFFDEFLKIK YEDVDSYIRN
     LSESPDNIKA FDDLYRKIMA MK
//