P20588 (T2F1_HAEIF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme HinfI
Short name=R.HinfI
EC=3.1.21.4

Alternative name(s):
Endonuclease HinfI
Type II restriction enzyme HinfI

Gene names

Name:

hinfIR

Organism

Haemophilus influenzae

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence GANTC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Type-2 restriction enzyme HinfI		PRO_0000077323

Sequences

	Sequence		Length	Mass (Da)
	P20588 [UniParc]. Last modified October 19, 2011. Version 3. Checksum: A65452B593FE8BD1	FASTA	262	30,912
	10 20 30 40 50 60 MNDEISDLIN QAVSNILINS SSENKLKKLI KTHDVKIHFV PRNYRIFGGI LQSMNIQFGN 70 80 90 100 110 120 FLEEFMTLLI KSDGRYDILE EYSGKKSNKF QLSTSNDNRI DQFISFCQRS DSINLDEEFP 130 140 150 160 170 180 KLLNEVKNDN DTNLSSISHD IDILFRNKET GVIYYLEVKY NDDHDTGKFV DINRKFIKTY 190 200 210 220 230 240 AYLVREFPNT EIKPILFFFN NKKMKGNIYV PENTNIRRGK SFFDEFLKIK YEDVDSYIRN 250 260 LSESPDNIKA FDDLYRKIMA MK

References

[1]	"Cloning and sequencing the HinfI restriction and modification genes." Chandrasegaran S., Lunnen K.D., Smith H.O., Wilson G.G. Gene 70:387-392(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RF.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M22862 Genomic DNA. Translation: AAA24988.1.
PIR	JT0390.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1154. HinfI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2F1_HAEIF

Accession

Primary (citable) accession number: P20588

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	October 19, 2011
Last modified:	April 3, 2013
This is version 56 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program