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Protein

Type-2 restriction enzyme HinfI

Gene

hinfIR

Organism
Haemophilus influenzae
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence GANTC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Enzyme and pathway databases

BRENDAi3.1.21.4. 2529.

Protein family/group databases

REBASEi1154. HinfI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HinfI (EC:3.1.21.4)
Short name:
R.HinfI
Alternative name(s):
Endonuclease HinfI
Type II restriction enzyme HinfI
Gene namesi
Name:hinfIR
OrganismiHaemophilus influenzae
Taxonomic identifieri727 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Type-2 restriction enzyme HinfIPRO_0000077323Add
BLAST

Sequencei

Sequence statusi: Complete.

P20588-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDEISDLIN QAVSNILINS SSENKLKKLI KTHDVKIHFV PRNYRIFGGI
60 70 80 90 100
LQSMNIQFGN FLEEFMTLLI KSDGRYDILE EYSGKKSNKF QLSTSNDNRI
110 120 130 140 150
DQFISFCQRS DSINLDEEFP KLLNEVKNDN DTNLSSISHD IDILFRNKET
160 170 180 190 200
GVIYYLEVKY NDDHDTGKFV DINRKFIKTY AYLVREFPNT EIKPILFFFN
210 220 230 240 250
NKKMKGNIYV PENTNIRRGK SFFDEFLKIK YEDVDSYIRN LSESPDNIKA
260
FDDLYRKIMA MK
Length:262
Mass (Da):30,912
Last modified:October 19, 2011 - v3
Checksum:iA65452B593FE8BD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22862 Genomic DNA. Translation: AAA24988.1.
PIRiJT0390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22862 Genomic DNA. Translation: AAA24988.1.
PIRiJT0390.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1154. HinfI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.21.4. 2529.

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing the HinfI restriction and modification genes."
    Chandrasegaran S., Lunnen K.D., Smith H.O., Wilson G.G.
    Gene 70:387-392(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RF.

Entry informationi

Entry nameiT2F1_HAEIF
AccessioniPrimary (citable) accession number: P20588
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 19, 2011
Last modified: April 1, 2015
This is version 60 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.