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P20588 (T2F1_HAEIF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HinfI

Short name=R.HinfI
EC=3.1.21.4
Alternative name(s):
Endonuclease HinfI
Type II restriction enzyme HinfI
Gene names
Name:hinfIR
OrganismHaemophilus influenzae
Taxonomic identifier727 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GANTC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 262262Type-2 restriction enzyme HinfI
PRO_0000077323

Sequences

Sequence LengthMass (Da)Tools
P20588 [UniParc].

Last modified October 19, 2011. Version 3.
Checksum: A65452B593FE8BD1

FASTA26230,912
        10         20         30         40         50         60 
MNDEISDLIN QAVSNILINS SSENKLKKLI KTHDVKIHFV PRNYRIFGGI LQSMNIQFGN 

        70         80         90        100        110        120 
FLEEFMTLLI KSDGRYDILE EYSGKKSNKF QLSTSNDNRI DQFISFCQRS DSINLDEEFP 

       130        140        150        160        170        180 
KLLNEVKNDN DTNLSSISHD IDILFRNKET GVIYYLEVKY NDDHDTGKFV DINRKFIKTY 

       190        200        210        220        230        240 
AYLVREFPNT EIKPILFFFN NKKMKGNIYV PENTNIRRGK SFFDEFLKIK YEDVDSYIRN 

       250        260 
LSESPDNIKA FDDLYRKIMA MK 

« Hide

References

[1]"Cloning and sequencing the HinfI restriction and modification genes."
Chandrasegaran S., Lunnen K.D., Smith H.O., Wilson G.G.
Gene 70:387-392(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: RF.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M22862 Genomic DNA. Translation: AAA24988.1.
PIRJT0390.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1154. HinfI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2F1_HAEIF
AccessionPrimary (citable) accession number: P20588
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 19, 2011
Last modified: April 16, 2014
This is version 59 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries