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P20588

- T2F1_HAEIF

UniProt

P20588 - T2F1_HAEIF

Protein

Type-2 restriction enzyme HinfI

Gene

hinfIR

Organism
Haemophilus influenzae
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 59 (16 Apr 2014)
      Sequence version 3 (19 Oct 2011)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GANTC and cleaves after G-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1154. HinfI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme HinfI (EC:3.1.21.4)
    Short name:
    R.HinfI
    Alternative name(s):
    Endonuclease HinfI
    Type II restriction enzyme HinfI
    Gene namesi
    Name:hinfIR
    OrganismiHaemophilus influenzae
    Taxonomic identifieri727 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 262262Type-2 restriction enzyme HinfIPRO_0000077323Add
    BLAST

    Sequencei

    Sequence statusi: Complete.

    P20588-1 [UniParc]FASTAAdd to Basket

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    MNDEISDLIN QAVSNILINS SSENKLKKLI KTHDVKIHFV PRNYRIFGGI    50
    LQSMNIQFGN FLEEFMTLLI KSDGRYDILE EYSGKKSNKF QLSTSNDNRI 100
    DQFISFCQRS DSINLDEEFP KLLNEVKNDN DTNLSSISHD IDILFRNKET 150
    GVIYYLEVKY NDDHDTGKFV DINRKFIKTY AYLVREFPNT EIKPILFFFN 200
    NKKMKGNIYV PENTNIRRGK SFFDEFLKIK YEDVDSYIRN LSESPDNIKA 250
    FDDLYRKIMA MK 262
    Length:262
    Mass (Da):30,912
    Last modified:October 19, 2011 - v3
    Checksum:iA65452B593FE8BD1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22862 Genomic DNA. Translation: AAA24988.1.
    PIRiJT0390.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22862 Genomic DNA. Translation: AAA24988.1 .
    PIRi JT0390.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1154. HinfI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing the HinfI restriction and modification genes."
      Chandrasegaran S., Lunnen K.D., Smith H.O., Wilson G.G.
      Gene 70:387-392(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: RF.

    Entry informationi

    Entry nameiT2F1_HAEIF
    AccessioniPrimary (citable) accession number: P20588
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: October 19, 2011
    Last modified: April 16, 2014
    This is version 59 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3