P20580 (TRPE_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component 1
EC=4.1.3.27

Alternative name(s):
Anthranilate synthase component I

Gene names

Name:	trpE
Ordered Locus Names:	PA0609

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II By similarity.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Anthranilate synthase component 1		PRO_0000154106

Sequences

	Sequence		Length	Mass (Da)
	P20580 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 60FEAABF37C2CD13	FASTA	492	54,555
	10 20 30 40 50 60 MNREEFLRLA ADGYNRIPLS FETLADFDTP LSIYLKLADA PNSYLLESVQ GGEKWGRYSI 70 80 90 100 110 120 IGLPCRTVLR VYDHQVRISI DGVETERFDC ADPLAFVEEF KARYQVPTVP GLPRFDGGLV 130 140 150 160 170 180 GYFGYDCVRY VEKRLATCPN PDPLGNPDIL LMVSDAVVVF DNLAGKIHAI VLADPSEENA 190 200 210 220 230 240 YERGQARLEE LLERLRQPIT PRRGLDLEAA QGREPAFRAS FTREDYENAV GRIKDYILAG 250 260 270 280 290 300 DCMQVVPSQR MSIEFKAAPI DLYRALRCFN PTPYMYFFNF GDFHVVGSSP EVLVRVEDGL 310 320 330 340 350 360 VTVRPIAGTR PRGINEEADL ALEQDLLSDA KEIAEHLMLI DLGRNDVGRV SDIGAVKVTE 370 380 390 400 410 420 KMVIERYSNV MHIVSNVTGQ LREGLSAMDA LRAILPAGTL SGAPKIRAME IIDELEPVKR 430 440 450 460 470 480 GVYGGAVGYL AWNGNMDTAI AIRTAVIKNG ELHVQAGGGI VADSVPALEW EETINKRRAM 490 FRAVALAEQS VE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequences and characterization of four early genes of the tryptophan pathway in Pseudomonas aeruginosa." Essar D.W., Eberly L., Han C.Y., Crawford I.P. J. Bacteriol. 172:853-866(1990) [PubMed: 2105306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genetic relationship between bacteriocins and bacteriophages." Nakayama K., Takashima K., Ishihara H., Shinomiya T., Kageyama M., Kanaya S., Ohnishi M., Murata T., Terawaki Y., Mori H., Hayashi T. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[4]	"Regulation of pyocin genes in Pseudomonas aeruginosa by positive (prtN) and negative (prtR) regulatory genes." Matsui H., Sano Y., Ishihara H., Shinomiya T. J. Bacteriol. 175:1257-1263(1993) [PubMed: 8444788] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 207-492.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33814 Genomic DNA. No translation available. AB030825 Genomic DNA. Translation: BAA83144.1. AE004091 Genomic DNA. Translation: AAG03998.1. D12706 Genomic DNA. Translation: BAA02200.1.
PIR	D35114.
RefSeq	NP_249300.1. NC_002516.2.
3D structure databases
ProteinModelPortal	P20580.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	879191.
GenomeReviews	Gene locus PA0609 in contig AE004091_GR.
KEGG	pae:PA0609.
NMPDR	fig|208964.1.peg.610.
PATRIC	19835490. VBIPseAer58763_0645.
Organism-specific databases
PseudoCAP	PA0609.
Phylogenomic databases
HOGENOM	HBG507440.
OMA	RTIYGRA.
ProtClustDB	PRK13565.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16005. PAER208964:PA0609-MONOMER.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR019999. Anth_synth_I. IPR006805. Anth_synth_I_N. IPR005256. Anth_synth_I_PabB. IPR015890. Chorismate-bd_C. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
KO	K01657.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
SUPFAM	SSF56322. TRPE_1_chor_bd. 1 hit.
TIGRFAMs	TIGR00564. TrpE_most. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPE_PSEAE

Accession

Primary (citable) accession number: P20580

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program