P20576 (TRPG_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component II
EC=4.1.3.27

Alternative name(s):
Glutamine amido-transferase

Gene names

Name:	trpG
Ordered Locus Names:	PA0649

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Contains 2 chains with different activities: component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Domain	Glutamine amidotransferase
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Anthranilate synthase component II		PRO_0000056895
Regions
	Domain	1 – 199	199	Glutamine amidotransferase type-1
Sites
	Active site	79	1	By similarity
	Active site	173	1	By similarity
	Active site	175	1	By similarity
Experimental info
	Sequence conflict	177	1	I → V in AAA25823. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P20576 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 730A4AA52F4348C1	FASTA	201	22,046
	10 20 30 40 50 60 MLLMIDNYDS FTYNLVQYFG ELKAEVKVVR NDELSVEQIE ALAPERIVLS PGPCTPNEAG 70 80 90 100 110 120 VSLAVIERFA GKLPLLGVCL GHQSIGQAFG GEVVRARQVM HGKTSPIHHK DLGVFAGLAN 130 140 150 160 170 180 PLTVTRYHSL VVKRESLPEC LEVTAWTQHA DGSLDEIMGV RHKTLNVEGV QFHPESILTE 190 200 QGHELLANFL RQQGGVRGEG N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequences and characterization of four early genes of the tryptophan pathway in Pseudomonas aeruginosa." Essar D.W., Eberly L., Han C.Y., Crawford I.P. J. Bacteriol. 172:853-866(1990) [PubMed: 2105306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33814 Genomic DNA. Translation: AAA25823.1. AE004091 Genomic DNA. Translation: AAG04038.1.
PIR	A35114. G83563.
RefSeq	NP_249340.1. NC_002516.2.
3D structure databases
ProteinModelPortal	P20576.
ModBase	Search...
Protein family/group databases
MEROPS	C26.A19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878400.
GenomeReviews	Gene locus PA0649 in contig AE004091_GR.
KEGG	pae:PA0649.
NMPDR	fig|208964.1.peg.650.
PATRIC	19835560. VBIPseAer58763_0680.
Organism-specific databases
PseudoCAP	PA0649.
Phylogenomic databases
HOGENOM	HBG292341.
OMA	SVDEIMG.
ProtClustDB	PRK05670.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16006. PAER208964:PA0649-MONOMER.
Family and domain databases
InterPro	IPR017926. GATASE_1. IPR006221. TrpG_papA. [Graphical view]
KO	K01658.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00566. TrpG_papA. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPG_PSEAE

Accession

Primary (citable) accession number: P20576

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	December 8, 2000
Last modified:	January 25, 2012
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program